Restriction map for
pFN21AE0154
(Circular) (Six-base) MAPSORT of: pFN21AE0154.seq Check: 3657 from: 1 to: 5599
pFN21AE0154 5599 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2150 2150
Size: 5599
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3409 278
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2942 1937
Size: 1005 4594
Acc65I G'GTAC_C
Cuts at: 2070 2920 2070
Size: 850 4749
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3662 5046 5314 1632
Size: 2030 1384 268 1917
Fragments arranged by size:
2030 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5402
AflIII A'CryG_T
Cuts at: 1203 4498 5475 1203
Size: 3295 977 1327
Fragments arranged by size:
3295 1327 977
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 4328 4328
Size: 5599
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2232 1184
Size: 1048 4551
AlwNI CAG_nnn'CTG
Cuts at: 3342 4914 3342
Size: 1572 4027
ApaLI G'TGCA_C
Cuts at: 3656 4812 3656
Size: 1156 4443
ApoI r'AATT_y
Cuts at: 1493 2185 2672 2908 3134 3286 5395 1493
Size: 692 487 236 226 152 2109 1697
Fragments arranged by size:
2109 1697 692 487 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 1870
Size: 73 981 4545
Fragments arranged by size:
4545 981 73
BamHI G'GATC_C
Cuts at: 2929 3296 2929
Size: 367 5232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 2070 2920 4375 618
Size: 325 217 627 225 58 850 1455 1842
Fragments arranged by size:
1842 1455 850 627 325 225 217 58
BanII G_rGCy'C
Cuts at: 1391 2918 1391
Size: 1527 4072
BbeI G_GCGC'C
Cuts at: 2016 2016
Size: 5599
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2898 928
Size: 971 999 3629
Fragments arranged by size:
3629 999 971
BbvCI CC'TCA_GC
Cuts at: 2522 2522
Size: 5599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3790 3824 1903
Size: 34 1853 34 3678
Fragments arranged by size:
3678 1853 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2469 2502 3491 4701 2469
Size: 33 989 1210 3367
Fragments arranged by size:
3367 1210 989 33
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4210 136
Size: 107 122 71 631 605 2538 1525
Fragments arranged by size:
2538 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5594 5594
Size: 5599
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 2581 2832 3660 4816 1790
Size: 791 251 828 1156 2573
Fragments arranged by size:
2573 1156 828 791 251
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5599
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2392 3330 4288
Size: 416 53 83 399 24 975 938 958
Cuts at: 4288 442
Size: 1753
Fragments arranged by size:
1753 975 958 938 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2392 4259 1239
Size: 989 164 1867 2579
Fragments arranged by size:
2579 1867 989 164
Bpu10I CC'TnA_GC
Cuts at: 2522 2522
Size: 5599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3724 4589 4887 3724
Size: 865 298 4436
Fragments arranged by size:
4436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4262 882
Size: 617 2763 2219
Fragments arranged by size:
2763 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2116 2389 3390 493
Size: 711 912 273 1001 2702
Fragments arranged by size:
2702 1001 912 711 273
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 3406 3788 4423
Size: 53 83 186 936 480 1393 382 635
Cuts at: 4423 275
Size: 1451
Fragments arranged by size:
1451 1393 936 635 480 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4032 4430 4704 4851 5262 4032
Size: 398 274 147 411 4369
Fragments arranged by size:
4369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2378 2408 1178
Size: 30 165 30 975 30 4369
Fragments arranged by size:
4369 975 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5599
BseYI C'CCAG_C
Cuts at: 2157 2495 4802 5298 2157
Size: 338 2307 496 2458
Fragments arranged by size:
2458 2307 496 338
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5026
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2182 3049 3810 4838 1540
Size: 275 186 181 867 761 1028 2301
Fragments arranged by size:
2301 1028 867 761 275 186 181
BsiHKAI G_wGCw'C
Cuts at: 2242 3660 3745 4816 2242
Size: 1418 85 1071 3025
Fragments arranged by size:
3025 1418 1071 85
BsmI GAATG_Cn'
Cuts at: 3110 3203 3110
Size: 93 5506
BsmBI CGTCTCn'nnnn_
Cuts at: 2059 2059
Size: 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2242 2581 2832 2918 3660 3745 4816
Size: 399 452 339 251 86 742 85 1071
Cuts at: 4816 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 452 399 339 251 86
85
BspEI T'CCGG_A
Cuts at: 5262 5262
Size: 5599
BspHI T'CATG_A
Cuts at: 2379 3488 5218 2379
Size: 1109 1730 2760
Fragments arranged by size:
2760 1730 1109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2150 2954 844
Size: 1306 804 3489
Fragments arranged by size:
3489 1306 804
BsrBI CCG'CTC
Cuts at: 1394 1823 3486 1394
Size: 429 1663 3507
Fragments arranged by size:
3507 1663 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4243 4430 1700
Size: 110 2433 187 2869
Fragments arranged by size:
2869 2433 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5599
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5599
BssSI C'ACGA_G
Cuts at: 3659 4671 3659
Size: 1012 4587
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5599
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5599
BstYI r'GATC_y
Cuts at: 1972 2929 3296 3681 3698 5150 5236 5248 5594
Size: 957 367 385 17 1452 86 12 346
Cuts at: 5594 1972
Size: 1977
Fragments arranged by size:
1977 1452 957 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2091 513
Size: 552 1026 4021
Fragments arranged by size:
4021 1026 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4860
Fragments arranged by size:
4860 683 56
BtsI GCAGTG_nn'
Cuts at: 2152 2808 2836 3115 3909 3929 2152
Size: 656 28 279 794 20 3822
Fragments arranged by size:
3822 794 656 279 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5564
DraI TTT'AAA
Cuts at: 2359 2511 2903 2359
Size: 152 392 5055
Fragments arranged by size:
5055 392 152
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2480 4606 809
Size: 507 1164 2126 1802
Fragments arranged by size:
2126 1802 1164 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2179 3046
Size: 54 1425 50 275 18 213 136 867
Cuts at: 3046 3935 5564 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 867 275 213 136 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2179 3046 1537
Size: 275 367 867 4090
Fragments arranged by size:
4090 867 367 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2359 2473 3529 1353
Size: 1006 114 1056 3423
Fragments arranged by size:
3423 1056 1006 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4186 4560 4706 387
Size: 1064 138 2597 374 146 1280
Fragments arranged by size:
2597 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2392 3662 4259 5046 5314 1239
Size: 393 596 164 1270 597 787 268 1524
Fragments arranged by size:
1524 1270 787 597 596 393 268 164
EcoRI G'AATT_C
Cuts at: 2672 2908 3286 2672
Size: 236 378 4985
Fragments arranged by size:
4985 378 236
EcoRV GAT'ATC
Cuts at: 2845 2845
Size: 5599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 5599
FspI TGC'GCA
Cuts at: 2265 4105 2265
Size: 1840 3759
HaeII r_GCGC'y
Cuts at: 1678 2016 4746 1678
Size: 338 2730 2531
Fragments arranged by size:
2730 2531 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4240 4272 4314 4346 1373
Size: 32 135 32 2668 32 42 32 2626
Fragments arranged by size:
2668 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2943 3189 669
Size: 988 281 1005 246 3079
Fragments arranged by size:
3079 1005 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5599
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KasI G'GCGC_C
Cuts at: 2012 2012
Size: 5599
KpnI G_GTAC'C
Cuts at: 2074 2924 2074
Size: 850 4749
MfeI C'AATT_G
Cuts at: 2350 3198 2350
Size: 848 4751
MluI A'CGCG_T
Cuts at: 5475 5475
Size: 5599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4713 4897 1305
Size: 3408 184 2007
Fragments arranged by size:
3408 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 10
Size: 54 1425 343 213 3564
Fragments arranged by size:
3564 1425 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3557 3916 4075 518
Size: 648 2391 359 159 2042
Fragments arranged by size:
2391 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2526 3015 3692 4840 5085 1606
Size: 642 278 489 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 489 278 245
NarI GG'CG_CC
Cuts at: 2013 2013
Size: 5599
NcoI C'CATG_G
Cuts at: 513 1065 2091 513
Size: 552 1026 4021
Fragments arranged by size:
4021 1026 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5599
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2957 4502 1132
Size: 97 1728 1545 2229
Fragments arranged by size:
2229 1728 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5599
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5599
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PmlI CAC'GTG
Cuts at: 2389 2389
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 5181 5213 3668
Size: 32 1481 32 4054
Fragments arranged by size:
4054 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5599
PsiI TTA'TAA
Cuts at: 2562 3169 2562
Size: 607 4992
PstI C_TGCA'G
Cuts at: 830 2951 830
Size: 2121 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5599
PvuII CAG'CTG
Cuts at: 2248 2526 2248
Size: 278 5321
SalI G'TCGA_C
Cuts at: 1936 2941 1936
Size: 1005 4594
SapI GCTCTTCn'nnn_
Cuts at: 2359 2359
Size: 5599
SbfI CC_TGCA'GG
Cuts at: 2951 2951
Size: 5599
ScaI AGT'ACT
Cuts at: 3847 5559 3847
Size: 1712 3887
SfcI C'TryA_G
Cuts at: 826 1045 2947 4082 4763 4954 826
Size: 219 1902 1135 681 191 1471
Fragments arranged by size:
1902 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2014 2014
Size: 5599
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5599
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3703 4604 4866 820
Size: 197 926 1760 901 262 1553
Fragments arranged by size:
1760 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2957 1132
Size: 1825 3774
SspI AAT'ATT
Cuts at: 5 52 3523 5
Size: 47 3471 2081
Fragments arranged by size:
3471 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5599
StyI C'CwwG_G
Cuts at: 513 1065 2040 2091 2617 3038 513
Size: 552 975 51 526 421 3074
Fragments arranged by size:
3074 975 552 526 421 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2149 3626 3643 3796 3981 4426 325
Size: 1255 569 1477 17 153 185 445 1498
Fragments arranged by size:
1498 1477 1255 569 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2608 3845
Size: 274 80 33 51 1443 345 286 1237
Cuts at: 3845 5557 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1237 345 286 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2378 2410 2469 2501 5559 5591 2378
Size: 32 59 32 3058 32 2386
Fragments arranged by size:
3058 2386 59 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5599
XbaI T'CTAG_A
Cuts at: 2884 2935 2884
Size: 51 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 3728 3728
Size: 5599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3407 276
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI XcmI