Restriction map for
pFN21AE0146
(Circular) (Six-base) MAPSORT of: pFN21AE0146.seq Check: 4707 from: 1 to: 5593
pFN21AE0146 5593 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3403 278
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2936 1937
Size: 999 4594
Acc65I G'GTAC_C
Cuts at: 2635 2667 2914 2635
Size: 32 247 5314
Fragments arranged by size:
5314 247 32
AclI AA'CG_TT
Cuts at: 1563 3720 4093 1563
Size: 2157 373 3063
Fragments arranged by size:
3063 2157 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2466 2715 3656 5040 5308 1632
Size: 834 249 941 1384 268 1917
Fragments arranged by size:
1917 1384 941 834 268 249
AflII C'TTAA_G
Cuts at: 820 1017 2876 820
Size: 197 1859 3537
Fragments arranged by size:
3537 1859 197
AflIII A'CryG_T
Cuts at: 1203 4492 5469 1203
Size: 3289 977 1327
Fragments arranged by size:
3289 1327 977
AgeI A'CCGG_T
Cuts at: 4424 4424
Size: 5593
AhdI GACnn_n'nnGTC
Cuts at: 4322 4322
Size: 5593
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5593
AlwNI CAG_nnn'CTG
Cuts at: 3336 4908 3336
Size: 1572 4021
ApaI G_GGCC'C
Cuts at: 2035 2041 2035
Size: 6 5587
ApaLI G'TGCA_C
Cuts at: 3650 4806 3650
Size: 1156 4437
ApoI r'AATT_y
Cuts at: 1493 2687 2902 3128 3280 5389 1493
Size: 1194 215 226 152 2109 1697
Fragments arranged by size:
2109 1697 1194 226 215 152
AseI AT'TA_AT
Cuts at: 160 1032 4147 160
Size: 872 3115 1606
Fragments arranged by size:
3115 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2918 1870
Size: 73 91 884 4545
Fragments arranged by size:
4545 884 91 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2294 2327 2294
Size: 33 5560
BamHI G'GATC_C
Cuts at: 2677 2785 2923 3290 2677
Size: 108 138 367 4980
Fragments arranged by size:
4980 367 138 108
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2635 2667 2914 4369 618
Size: 325 217 627 848 32 247 1455 1842
Fragments arranged by size:
1842 1455 848 627 325 247 217 32
BanII G_rGCy'C
Cuts at: 1391 2035 2041 2912 1391
Size: 644 6 871 4072
Fragments arranged by size:
4072 871 644 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2125 928
Size: 971 226 4396
Fragments arranged by size:
4396 971 226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3784 3818 1903
Size: 34 1847 34 3678
Fragments arranged by size:
3678 1847 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3485 4695 3485
Size: 1210 4383
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4204 136
Size: 107 122 71 631 605 2532 1525
Fragments arranged by size:
2532 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5588 5588
Size: 5593
BlpI GC'TnA_GC
Cuts at: 3010 3010
Size: 5593
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2041 3654 4810 1790
Size: 245 6 1613 1156 2573
Fragments arranged by size:
2573 1613 1156 245 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5593
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3324 4282 442
Size: 416 53 83 399 24 1907 958 1753
Fragments arranged by size:
1907 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2799 4253 1239
Size: 1560 1454 2579
Fragments arranged by size:
2579 1560 1454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3718 4583 4881 3718
Size: 865 298 4430
Fragments arranged by size:
4430 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4256 882
Size: 617 2757 2219
Fragments arranged by size:
2757 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3384 493
Size: 711 2180 2702
Fragments arranged by size:
2702 2180 711
BsaBI GATnn'nnATC
Cuts at: 2868 5587 2868
Size: 2719 2874
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2275 3400 3782 4417
Size: 53 83 186 936 742 1125 382 635
Cuts at: 4417 275
Size: 1451
Fragments arranged by size:
1451 1125 936 742 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2110 4026 4424 4698 4845 5256 2110
Size: 1916 398 274 147 411 2447
Fragments arranged by size:
2447 1916 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2125 2155 1178
Size: 30 165 30 722 30 4616
Fragments arranged by size:
4616 722 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 1186
Size: 1258 4335
BseYI C'CCAG_C
Cuts at: 2361 4796 5292 2361
Size: 2435 496 2662
Fragments arranged by size:
2662 2435 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5020
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3043 3804 4832 1540
Size: 275 186 1042 761 1028 2301
Fragments arranged by size:
2301 1042 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3654 3739 4810 3654
Size: 85 1071 4437
Fragments arranged by size:
4437 1071 85
BsmI GAATG_Cn'
Cuts at: 2619 3104 3197 2619
Size: 485 93 5015
Fragments arranged by size:
5015 485 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2041 2912 3654 3739 4810 1391
Size: 399 245 6 871 742 85 1071 2174
Fragments arranged by size:
2174 1071 871 742 399 245 85 6
BspEI T'CCGG_A
Cuts at: 5256 5256
Size: 5593
BspHI T'CATG_A
Cuts at: 3482 5212 3482
Size: 1730 3863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2948 844
Size: 2104 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3480 1394
Size: 429 1657 3507
Fragments arranged by size:
3507 1657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4088 4262 65
Size: 1183 2840 174 1396
Fragments arranged by size:
2840 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 4237 4424 1700
Size: 110 199 2228 187 2869
Fragments arranged by size:
2869 2228 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5593
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5593
BssSI C'ACGA_G
Cuts at: 3653 4665 3653
Size: 1012 4581
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5593
BstBI TT'CG_AA
Cuts at: 4397 4397
Size: 5593
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5593
BstYI r'GATC_y
Cuts at: 1972 2677 2785 2923 3290 3675 3692 5144 5230
Size: 705 108 138 367 385 17 1452 86
Cuts at: 5230 5242 5588 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 705 385 367 346 138 108
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2662 513
Size: 552 939 658 3444
Fragments arranged by size:
3444 939 658 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4854
Fragments arranged by size:
4854 683 56
BtsI GCAGTG_nn'
Cuts at: 3109 3903 3923 3109
Size: 794 20 4779
Fragments arranged by size:
4779 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5593
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5558
DraI TTT'AAA
Cuts at: 2897 2897
Size: 5593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2127 4600 809
Size: 507 811 2473 1802
Fragments arranged by size:
2473 1802 811 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2076 2835
Size: 54 1425 50 275 18 177 69 759
Cuts at: 2835 3040 3929 5558 8
Size: 205 889 1629 43
Fragments arranged by size:
1629 1425 889 759 275 205 177 69
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3040 1537
Size: 275 1228 4090
Fragments arranged by size:
4090 1228 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2131 2765 3523 1353
Size: 778 634 758 3423
Fragments arranged by size:
3423 778 758 634
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2690 4180 4554 4700 387
Size: 1064 138 1101 1490 374 146 1280
Fragments arranged by size:
1490 1280 1101 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2466 2715 2799 3656 4253 5040 5308
Size: 393 834 249 84 857 597 787 268
Cuts at: 5308 1239
Size: 1524
Fragments arranged by size:
1524 857 834 787 597 393 268 249
84
EcoO109I rG'GnC_Cy
Cuts at: 2031 2038 2056 2031
Size: 7 18 5568
Fragments arranged by size:
5568 18 7
EcoRI G'AATT_C
Cuts at: 2902 3280 2902
Size: 378 5215
EcoRV GAT'ATC
Cuts at: 2743 2743
Size: 5593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2720 2720
Size: 5593
FspI TGC'GCA
Cuts at: 4099 4099
Size: 5593
HaeII r_GCGC'y
Cuts at: 1678 4740 1678
Size: 3062 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2694 2726 4234 4266 4308
Size: 32 135 32 1122 32 1508 32 42
Cuts at: 4308 4340 1373
Size: 32 2626
Fragments arranged by size:
2626 1508 1122 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2345 2937 3183 669
Size: 988 281 407 592 246 3079
Fragments arranged by size:
3079 988 592 407 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5593
HpaI GTT'AAC
Cuts at: 3183 3183
Size: 5593
KpnI G_GTAC'C
Cuts at: 2639 2671 2918 2639
Size: 32 247 5314
Fragments arranged by size:
5314 247 32
MfeI C'AATT_G
Cuts at: 3192 3192
Size: 5593
MluI A'CGCG_T
Cuts at: 5469 5469
Size: 5593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2111 4707 4891 1305
Size: 806 2596 184 2007
Fragments arranged by size:
2596 2007 806 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2078 10
Size: 54 1425 343 246 3525
Fragments arranged by size:
3525 1425 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3551 3910 4069 518
Size: 648 2385 359 159 2042
Fragments arranged by size:
2385 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2422 3009 3686 4834 5079 1606
Size: 816 587 677 1148 245 2120
Fragments arranged by size:
2120 1148 816 677 587 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4102
Fragments arranged by size:
4102 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5593
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5593
NotI GC'GGCC_GC
Cuts at: 3040 3040
Size: 5593
NspI r_CATG'y
Cuts at: 1132 1229 2951 4496 1132
Size: 97 1722 1545 2229
Fragments arranged by size:
2229 1722 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5593
PciI A'CATG_T
Cuts at: 4492 4492
Size: 5593
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5593
PfoI T'CCnGG_A
Cuts at: 2815 2815
Size: 5593
PmeI GTTT'AAAC
Cuts at: 2897 2897
Size: 5593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 5175 5207 3662
Size: 32 1481 32 4048
Fragments arranged by size:
4048 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5593
PsiI TTA'TAA
Cuts at: 3163 3163
Size: 5593
PspOMI G'GGCC_C
Cuts at: 2031 2037 2031
Size: 6 5587
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 5561
PstI C_TGCA'G
Cuts at: 830 2310 2945 830
Size: 1480 635 3478
Fragments arranged by size:
3478 1480 635
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5593
PvuII CAG'CTG
Cuts at: 2422 2422
Size: 5593
SalI G'TCGA_C
Cuts at: 1936 2935 1936
Size: 999 4594
SbfI CC_TGCA'GG
Cuts at: 2945 2945
Size: 5593
ScaI AGT'ACT
Cuts at: 3841 5553 3841
Size: 1712 3881
SfcI C'TryA_G
Cuts at: 826 1045 2306 2529 2859 2941 4076 4757 4948
Size: 219 1261 223 330 82 1135 681 191
Cuts at: 4948 826
Size: 1471
Fragments arranged by size:
1471 1261 1135 681 330 223 219 191
82
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5593
SmaI CCC'GGG
Cuts at: 1872 2036 2920 1872
Size: 164 884 4545
Fragments arranged by size:
4545 884 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2876 3697 4598 4860 820
Size: 197 926 933 821 901 262 1553
Fragments arranged by size:
1553 933 926 901 821 262 197
SnaBI TAC'GTA
Cuts at: 493 3384 493
Size: 2891 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5593
SphI G_CATG'C
Cuts at: 1132 2951 1132
Size: 1819 3774
SrfI GCCC'GGGC
Cuts at: 2036 2036
Size: 5593
SspI AAT'ATT
Cuts at: 5 52 2464 3517 5
Size: 47 2412 1053 2081
Fragments arranged by size:
2412 2081 1053 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5593
StyI C'CwwG_G
Cuts at: 513 1065 2004 2789 3032 513
Size: 552 939 785 243 3074
Fragments arranged by size:
3074 939 785 552 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2812 3620 3637 3790 3975 4420 325
Size: 1255 1232 808 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1232 808 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2470 2774 3839
Size: 274 80 33 51 1443 493 304 1065
Cuts at: 3839 5551 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1065 493 304 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2811 2843 5553 5585 2811
Size: 32 2710 32 2819
Fragments arranged by size:
2819 2710 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5593
XbaI T'CTAG_A
Cuts at: 2929 2929
Size: 5593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2369 2369
Size: 5593
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5593
XmaI C'CCGG_G
Cuts at: 1870 2034 2918 1870
Size: 164 884 4545
Fragments arranged by size:
4545 884 164
XmnI GAAnn'nnTTC
Cuts at: 3722 3722
Size: 5593
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3401 276
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SwaI