Restriction map for
pFN21AE0145
(Circular) (Six-base) MAPSORT of: pFN21AE0145.seq Check: 9893 from: 1 to: 5080
pFN21AE0145 5080 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2890 278
Size: 53 83 186 936 1354 2468
Fragments arranged by size:
2468 1354 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2423 1937
Size: 486 4594
Acc65I G'GTAC_C
Cuts at: 2401 2401
Size: 5080
AclI AA'CG_TT
Cuts at: 1563 3207 3580 1563
Size: 1644 373 3063
Fragments arranged by size:
3063 1644 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3143 4527 4795 1632
Size: 1511 1384 268 1917
Fragments arranged by size:
1917 1511 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4883
AflIII A'CryG_T
Cuts at: 1203 2271 3979 4956 1203
Size: 1068 1708 977 1327
Fragments arranged by size:
1708 1327 1068 977
AgeI A'CCGG_T
Cuts at: 3911 3911
Size: 5080
AhdI GACnn_n'nnGTC
Cuts at: 2182 3809 2182
Size: 1627 3453
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5080
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5080
AlwNI CAG_nnn'CTG
Cuts at: 2823 4395 2823
Size: 1572 3508
ApaLI G'TGCA_C
Cuts at: 3137 4293 3137
Size: 1156 3924
ApoI r'AATT_y
Cuts at: 1493 2389 2615 2767 4876 1493
Size: 896 226 152 2109 1697
Fragments arranged by size:
2109 1697 896 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3634 160
Size: 872 2602 1606
Fragments arranged by size:
2602 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5080
AvaI C'yCGr_G
Cuts at: 1870 1943 2405 1870
Size: 73 462 4545
Fragments arranged by size:
4545 462 73
BamHI G'GATC_C
Cuts at: 2410 2777 2410
Size: 367 4713
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2401 3856 618
Size: 325 217 627 614 1455 1842
Fragments arranged by size:
1842 1455 627 614 325 217
BanII G_rGCy'C
Cuts at: 1391 2110 2399 1391
Size: 719 289 4072
Fragments arranged by size:
4072 719 289
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2053 928
Size: 971 154 3955
Fragments arranged by size:
3955 971 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2113 2147 3271 3305 1903
Size: 34 176 34 1124 34 3678
Fragments arranged by size:
3678 1124 176 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2972 4182 2972
Size: 1210 3870
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5080
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3691 136
Size: 107 122 71 631 605 2019 1525
Fragments arranged by size:
2019 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5075 5075
Size: 5080
BlpI GC'TnA_GC
Cuts at: 2497 2497
Size: 5080
Bme1580I G_kGCm'C
Cuts at: 1790 3141 4297 1790
Size: 1351 1156 2573
Fragments arranged by size:
2573 1351 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5080
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2811 3769 442
Size: 416 53 83 399 24 1394 958 1753
Fragments arranged by size:
1753 1394 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3740 1239
Size: 2501 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3205 4070 4368 3205
Size: 865 298 3917
Fragments arranged by size:
3917 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3743 882
Size: 617 2244 2219
Fragments arranged by size:
2244 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2272 2871 493
Size: 711 1068 599 2702
Fragments arranged by size:
2702 1068 711 599
BsaBI GATnn'nnATC
Cuts at: 2081 5074 2081
Size: 2993 2087
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2887 3269 3904
Size: 53 83 186 936 525 829 382 635
Cuts at: 3904 275
Size: 1451
Fragments arranged by size:
1451 936 829 635 525 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3513 3911 4185 4332 4743 3513
Size: 398 274 147 411 3850
Fragments arranged by size:
3850 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 1178
Size: 30 165 30 630 30 4195
Fragments arranged by size:
4195 630 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5080
BseYI C'CCAG_C
Cuts at: 2304 4283 4779 2304
Size: 1979 496 2605
Fragments arranged by size:
2605 1979 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4507
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2530 3291 4319 1540
Size: 275 186 529 761 1028 2301
Fragments arranged by size:
2301 1028 761 529 275 186
BsiHKAI G_wGCw'C
Cuts at: 3141 3226 4297 3141
Size: 85 1071 3924
Fragments arranged by size:
3924 1071 85
BsmI GAATG_Cn'
Cuts at: 2591 2684 2591
Size: 93 4987
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2399 3141 3226 4297 1391
Size: 399 320 289 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 320 289 85
BspEI T'CCGG_A
Cuts at: 4743 4743
Size: 5080
BspHI T'CATG_A
Cuts at: 2969 4699 2969
Size: 1730 3350
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2435 844
Size: 1591 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2100 2967 1394
Size: 429 277 867 3507
Fragments arranged by size:
3507 867 429 277
BsrDI GCAATG_nn'
Cuts at: 65 1248 3575 3749 65
Size: 1183 2327 174 1396
Fragments arranged by size:
2327 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3724 3911 1700
Size: 110 1914 187 2869
Fragments arranged by size:
2869 1914 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5080
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5080
BssSI C'ACGA_G
Cuts at: 3140 4152 3140
Size: 1012 4068
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5080
BstBI TT'CG_AA
Cuts at: 3884 3884
Size: 5080
BstEII G'GTnAC_C
Cuts at: 1184 2356 1184
Size: 1172 3908
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5080
BstYI r'GATC_y
Cuts at: 1972 2410 2777 3162 3179 4631 4717 4729 5075
Size: 438 367 385 17 1452 86 12 346
Cuts at: 5075 1972
Size: 1977
Fragments arranged by size:
1977 1452 438 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2286 513
Size: 552 939 282 3307
Fragments arranged by size:
3307 939 552 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4341
Fragments arranged by size:
4341 683 56
BtsI GCAGTG_nn'
Cuts at: 2596 3390 3410 2596
Size: 794 20 4266
Fragments arranged by size:
4266 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5080
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5045
DraI TTT'AAA
Cuts at: 2384 2384
Size: 5080
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2354 4087 809
Size: 507 1038 1733 1802
Fragments arranged by size:
1802 1733 1038 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2527 3416 5045
Size: 54 1425 50 275 18 697 889 1629
Cuts at: 5045 8
Size: 43
Fragments arranged by size:
1629 1425 889 697 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2527 1537
Size: 275 715 4090
Fragments arranged by size:
4090 715 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3010 1353
Size: 1657 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 3667 4041 4187 387
Size: 1064 138 441 1637 374 146 1280
Fragments arranged by size:
1637 1280 1064 441 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3143 3740 4527 4795 1239
Size: 393 1511 597 787 268 1524
Fragments arranged by size:
1524 1511 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2389 2767 2389
Size: 378 4702
FspI TGC'GCA
Cuts at: 3586 3586
Size: 5080
HaeII r_GCGC'y
Cuts at: 1678 4227 1678
Size: 2549 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2168 2200 3721
Size: 32 135 32 461 32 103 32 1521
Cuts at: 3721 3753 3795 3827 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1521 461 135 103 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2424 2670 669
Size: 988 281 486 246 3079
Fragments arranged by size:
3079 988 486 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5080
HpaI GTT'AAC
Cuts at: 2670 2670
Size: 5080
KpnI G_GTAC'C
Cuts at: 2405 2405
Size: 5080
MfeI C'AATT_G
Cuts at: 2679 2679
Size: 5080
MluI A'CGCG_T
Cuts at: 4956 4956
Size: 5080
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2076 4194 4378 1305
Size: 771 2118 184 2007
Fragments arranged by size:
2118 2007 771 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3258
Fragments arranged by size:
3258 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3038 3397 3556 518
Size: 648 843 1029 359 159 2042
Fragments arranged by size:
2042 1029 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2496 3173 4321 4566 1606
Size: 890 677 1148 245 2120
Fragments arranged by size:
2120 1148 890 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2286 513
Size: 552 939 282 3307
Fragments arranged by size:
3307 939 552 282
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5080
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5080
NotI GC'GGCC_GC
Cuts at: 2527 2527
Size: 5080
NspI r_CATG'y
Cuts at: 1132 1229 2197 2438 3983 1132
Size: 97 968 241 1545 2229
Fragments arranged by size:
2229 1545 968 241 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5080
PciI A'CATG_T
Cuts at: 3979 3979
Size: 5080
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5080
PmeI GTTT'AAAC
Cuts at: 2384 2384
Size: 5080
PmlI CAC'GTG
Cuts at: 2272 2272
Size: 5080
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 4662 4694 3149
Size: 32 1481 32 3535
Fragments arranged by size:
3535 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5080
PsiI TTA'TAA
Cuts at: 2650 2650
Size: 5080
PstI C_TGCA'G
Cuts at: 830 2119 2432 830
Size: 1289 313 3478
Fragments arranged by size:
3478 1289 313
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5080
SalI G'TCGA_C
Cuts at: 1936 2422 1936
Size: 486 4594
SbfI CC_TGCA'GG
Cuts at: 2432 2432
Size: 5080
ScaI AGT'ACT
Cuts at: 3328 5040 3328
Size: 1712 3368
SfcI C'TryA_G
Cuts at: 826 1045 2115 2428 3563 4244 4435 826
Size: 219 1070 313 1135 681 191 1471
Fragments arranged by size:
1471 1135 1070 681 313 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5080
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5080
SmaI CCC'GGG
Cuts at: 1872 2407 1872
Size: 535 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3184 4085 4347 820
Size: 197 926 1241 901 262 1553
Fragments arranged by size:
1553 1241 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2871 493
Size: 2378 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5080
SphI G_CATG'C
Cuts at: 1132 2438 1132
Size: 1306 3774
SspI AAT'ATT
Cuts at: 5 52 3004 5
Size: 47 2952 2081
Fragments arranged by size:
2952 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5080
StyI C'CwwG_G
Cuts at: 513 1065 2004 2286 2519 513
Size: 552 939 282 233 3074
Fragments arranged by size:
3074 939 552 282 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3107 3124 3277 3462 3907 325
Size: 1255 1527 17 153 185 445 1498
Fragments arranged by size:
1527 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3326 5038 96
Size: 274 80 33 51 1443 1349 1712 138
Fragments arranged by size:
1712 1443 1349 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5040 5072 5040
Size: 32 5048
Tth111I GACn'n_nGTC
Cuts at: 1316 2057 1316
Size: 741 4339
XbaI T'CTAG_A
Cuts at: 2416 2416
Size: 5080
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5080
XmaI C'CCGG_G
Cuts at: 1870 2405 1870
Size: 535 4545
XmnI GAAnn'nnTTC
Cuts at: 3209 3209
Size: 5080
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2888 276
Size: 53 83 186 936 1354 2468
Fragments arranged by size:
2468 1354 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI