Restriction map for
pFN21AE0142
(Circular) (Six-base) MAPSORT of: pFN21AE0142.seq Check: 8538 from: 1 to: 5242
pFN21AE0142 5242 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2410 2410
Size: 5242
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3052 278
Size: 53 83 186 936 1516 2468
Fragments arranged by size:
2468 1516 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2585 1937
Size: 648 4594
Acc65I G'GTAC_C
Cuts at: 2563 2563
Size: 5242
AclI AA'CG_TT
Cuts at: 1563 3369 3742 1563
Size: 1806 373 3063
Fragments arranged by size:
3063 1806 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3305 4689 4957 1632
Size: 1673 1384 268 1917
Fragments arranged by size:
1917 1673 1384 268
AfeI AGC'GCT
Cuts at: 2409 2409
Size: 5242
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5045
AflIII A'CryG_T
Cuts at: 1203 4141 5118 1203
Size: 2938 977 1327
Fragments arranged by size:
2938 1327 977
AgeI A'CCGG_T
Cuts at: 4073 4073
Size: 5242
AhdI GACnn_n'nnGTC
Cuts at: 3971 3971
Size: 5242
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2370 2486 1184
Size: 1186 116 3940
Fragments arranged by size:
3940 1186 116
AlwNI CAG_nnn'CTG
Cuts at: 2528 2985 4557 2528
Size: 457 1572 3213
Fragments arranged by size:
3213 1572 457
ApaLI G'TGCA_C
Cuts at: 3299 4455 3299
Size: 1156 4086
ApoI r'AATT_y
Cuts at: 1493 2551 2777 2929 5038 1493
Size: 1058 226 152 2109 1697
Fragments arranged by size:
2109 1697 1058 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3796 160
Size: 872 2764 1606
Fragments arranged by size:
2764 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5242
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 1870
Size: 73 624 4545
Fragments arranged by size:
4545 624 73
BamHI G'GATC_C
Cuts at: 2572 2939 2572
Size: 367 4875
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2310 2431 2444 2563 4018
Size: 325 217 627 523 121 13 119 1455
Cuts at: 4018 618
Size: 1842
Fragments arranged by size:
1842 1455 627 523 325 217 121 119
13
BanII G_rGCy'C
Cuts at: 1391 2561 1391
Size: 1170 4072
BbeI G_GCGC'C
Cuts at: 2314 2435 2448 2314
Size: 121 13 5108
Fragments arranged by size:
5108 121 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3433 3467 1903
Size: 34 1496 34 3678
Fragments arranged by size:
3678 1496 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3134 4344 3134
Size: 1210 4032
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5242
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3853 136
Size: 107 122 71 631 605 2181 1525
Fragments arranged by size:
2181 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5237 5237
Size: 5242
BlpI GC'TnA_GC
Cuts at: 2659 2659
Size: 5242
Bme1580I G_kGCm'C
Cuts at: 1790 2201 3303 4459 1790
Size: 411 1102 1156 2573
Fragments arranged by size:
2573 1156 1102 411
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5242
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2164 2973 3931
Size: 416 53 83 399 24 747 809 958
Cuts at: 3931 442
Size: 1753
Fragments arranged by size:
1753 958 809 747 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 2471 3902 1239
Size: 1082 150 1431 2579
Fragments arranged by size:
2579 1431 1082 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3367 4232 4530 3367
Size: 865 298 4079
Fragments arranged by size:
4079 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3905 882
Size: 617 2406 2219
Fragments arranged by size:
2406 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3033 493
Size: 711 1829 2702
Fragments arranged by size:
2702 1829 711
BsaBI GATnn'nnATC
Cuts at: 2180 5236 2180
Size: 3056 2186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2311 2432 2445 3049
Size: 53 83 186 936 778 121 13 604
Cuts at: 3049 3431 4066 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 778 635 604 382 186 121
83 53 13
BsaWI w'CCGG_w
Cuts at: 3675 4073 4347 4494 4905 3675
Size: 398 274 147 411 4012
Fragments arranged by size:
4012 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5017
Fragments arranged by size:
5017 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2354 2357 1186
Size: 1051 117 3 4071
Fragments arranged by size:
4071 1051 117 3
BseYI C'CCAG_C
Cuts at: 2239 2254 2487 4445 4941 2239
Size: 15 233 1958 496 2540
Fragments arranged by size:
2540 1958 496 233 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2444 1163
Size: 573 708 3961
Fragments arranged by size:
3961 708 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2506 2692 3453 4481 1540
Size: 275 186 505 186 761 1028 2301
Fragments arranged by size:
2301 1028 761 505 275 186 186
BsiHKAI G_wGCw'C
Cuts at: 3303 3388 4459 3303
Size: 85 1071 4086
Fragments arranged by size:
4086 1071 85
BsmI GAATG_Cn'
Cuts at: 2753 2846 2753
Size: 93 5149
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2561 3303 3388 4459 1391
Size: 399 411 360 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 411 399 360 85
BspEI T'CCGG_A
Cuts at: 4905 4905
Size: 5242
BspHI T'CATG_A
Cuts at: 3131 4861 3131
Size: 1730 3512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2047 2410 2597 844
Size: 1203 363 187 3489
Fragments arranged by size:
3489 1203 363 187
BsrBI CCG'CTC
Cuts at: 1394 1823 3129 1394
Size: 429 1306 3507
Fragments arranged by size:
3507 1306 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3737 3911 65
Size: 1183 2489 174 1396
Fragments arranged by size:
2489 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2137 2505 3886 4073 1700
Size: 110 327 368 1381 187 2869
Fragments arranged by size:
2869 1381 368 327 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5242
BssHII G'CGCG_C
Cuts at: 1925 2482 1925
Size: 557 4685
BssSI C'ACGA_G
Cuts at: 3302 4314 3302
Size: 1012 4230
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2479 2528 794
Size: 1685 49 3508
Fragments arranged by size:
3508 1685 49
BstBI TT'CG_AA
Cuts at: 4046 4046
Size: 5242
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5242
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5242
BstYI r'GATC_y
Cuts at: 1972 2572 2939 3324 3341 4793 4879 4891 5237
Size: 600 367 385 17 1452 86 12 346
Cuts at: 5237 1972
Size: 1977
Fragments arranged by size:
1977 1452 600 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3751
Fragments arranged by size:
3751 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4503
Fragments arranged by size:
4503 683 56
BtsI GCAGTG_nn'
Cuts at: 2529 2758 3552 3572 2529
Size: 229 794 20 4199
Fragments arranged by size:
4199 794 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5207
DraI TTT'AAA
Cuts at: 2546 2546
Size: 5242
DraIII CAC_nnn'GTG
Cuts at: 2158 2524 2158
Size: 366 4876
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4249 809
Size: 507 2933 1802
Fragments arranged by size:
2933 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2503 2689 3578
Size: 54 1425 50 275 18 673 186 889
Cuts at: 3578 5207 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 673 275 186 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2503 2689 1537
Size: 275 691 186 4090
Fragments arranged by size:
4090 691 275 186
EarI CTCTTCn'nnn_
Cuts at: 1353 3172 1353
Size: 1819 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3829 4203 4349 387
Size: 1064 138 2240 374 146 1280
Fragments arranged by size:
2240 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2471 3305 3902 4689 4957 1239
Size: 393 689 150 834 597 787 268 1524
Fragments arranged by size:
1524 834 787 689 597 393 268 150
EcoRI G'AATT_C
Cuts at: 2551 2929 2551
Size: 378 4864
FspI TGC'GCA
Cuts at: 2180 2462 3748 2180
Size: 282 1286 3674
Fragments arranged by size:
3674 1286 282
FspAI rTGC'GCAy
Cuts at: 2180 2180
Size: 5242
HaeII r_GCGC'y
Cuts at: 1678 2314 2332 2396 2402 2411 2435 2448 4389
Size: 636 18 64 6 9 24 13 1941
Cuts at: 4389 1678
Size: 2531
Fragments arranged by size:
2531 1941 636 64 24 18 13 9
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3883 3915 3957 3989 1373
Size: 32 135 32 2311 32 42 32 2626
Fragments arranged by size:
2626 2311 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2586 2832 669
Size: 988 281 648 246 3079
Fragments arranged by size:
3079 988 648 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5242
HpaI GTT'AAC
Cuts at: 2832 2832
Size: 5242
KasI G'GCGC_C
Cuts at: 2310 2431 2444 2310
Size: 121 13 5108
Fragments arranged by size:
5108 121 13
KpnI G_GTAC'C
Cuts at: 2567 2567
Size: 5242
MfeI C'AATT_G
Cuts at: 2841 2841
Size: 5242
MluI A'CGCG_T
Cuts at: 5118 5118
Size: 5242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4356 4540 1305
Size: 3051 184 2007
Fragments arranged by size:
3051 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3420
Fragments arranged by size:
3420 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3200 3559 3718 518
Size: 648 843 1191 359 159 2042
Fragments arranged by size:
2042 1191 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2215 2658 3335 4483 4728 1606
Size: 609 443 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 609 443 245
NaeI GCC'GGC
Cuts at: 2139 2139
Size: 5242
NarI GG'CG_CC
Cuts at: 2311 2432 2445 2311
Size: 121 13 5108
Fragments arranged by size:
5108 121 13
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3751
Fragments arranged by size:
3751 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5242
NgoMIV G'CCGG_C
Cuts at: 2137 2137
Size: 5242
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5242
NotI GC'GGCC_GC
Cuts at: 2689 2689
Size: 5242
NspI r_CATG'y
Cuts at: 1132 1229 2600 4145 1132
Size: 97 1371 1545 2229
Fragments arranged by size:
2229 1545 1371 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5242
PciI A'CATG_T
Cuts at: 4141 4141
Size: 5242
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5242
PmeI GTTT'AAAC
Cuts at: 2546 2546
Size: 5242
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 4824 4856 3311
Size: 32 1481 32 3697
Fragments arranged by size:
3697 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5242
PsiI TTA'TAA
Cuts at: 2812 2812
Size: 5242
PstI C_TGCA'G
Cuts at: 830 2415 2594 830
Size: 1585 179 3478
Fragments arranged by size:
3478 1585 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5242
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 5242
SalI G'TCGA_C
Cuts at: 1936 2584 1936
Size: 648 4594
SbfI CC_TGCA'GG
Cuts at: 2594 2594
Size: 5242
ScaI AGT'ACT
Cuts at: 2306 3490 5202 2306
Size: 1184 1712 2346
Fragments arranged by size:
2346 1712 1184
SfcI C'TryA_G
Cuts at: 826 1045 2411 2590 3725 4406 4597 826
Size: 219 1366 179 1135 681 191 1471
Fragments arranged by size:
1471 1366 1135 681 219 191 179
SfoI GGC'GCC
Cuts at: 2312 2433 2446 2312
Size: 121 13 5108
Fragments arranged by size:
5108 121 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5242
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5242
SmaI CCC'GGG
Cuts at: 1872 2569 1872
Size: 697 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3346 4247 4509 820
Size: 197 926 1403 901 262 1553
Fragments arranged by size:
1553 1403 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3033 493
Size: 2540 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5242
SphI G_CATG'C
Cuts at: 1132 2600 1132
Size: 1468 3774
SspI AAT'ATT
Cuts at: 5 52 3166 5
Size: 47 3114 2081
Fragments arranged by size:
3114 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5242
StyI C'CwwG_G
Cuts at: 513 1065 2004 2681 513
Size: 552 939 677 3074
Fragments arranged by size:
3074 939 677 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2146 3269 3286 3439 3624 4069 325
Size: 1255 566 1123 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1123 566 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 3488 5200
Size: 274 80 33 51 1443 327 1184 1712
Cuts at: 5200 96
Size: 138
Fragments arranged by size:
1712 1443 1184 327 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5202 5234 5202
Size: 32 5210
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5242
XbaI T'CTAG_A
Cuts at: 2578 2578
Size: 5242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2275 2275
Size: 5242
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5242
XmaI C'CCGG_G
Cuts at: 1870 2567 1870
Size: 697 4545
XmnI GAAnn'nnTTC
Cuts at: 3371 3371
Size: 5242
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3050 276
Size: 53 83 186 936 1516 2468
Fragments arranged by size:
2468 1516 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI RsrII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI