Restriction map for
pFN21AE0138
(Circular) (Six-base) MAPSORT of: pFN21AE0138.seq Check: 5405 from: 1 to: 5515
pFN21AE0138 5515 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2196 2196
Size: 5515
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 2020 2509 2529 3258 4830 2020
Size: 489 20 729 1572 2705
Fragments arranged by size:
2705 1572 729 489 20
ApaI G_GGCC'C
Cuts at: 2790 2790
Size: 5515
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2162 2781 2840 1870
Size: 73 219 619 59 4545
Fragments arranged by size:
4545 619 219 73 59
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2167 2281 2836 4291 618
Size: 325 217 627 380 114 555 1455 1842
Fragments arranged by size:
1842 1455 627 555 380 325 217 114
BanII G_rGCy'C
Cuts at: 1391 2790 2834 1391
Size: 1399 44 4072
Fragments arranged by size:
4072 1399 44
BbeI G_GCGC'C
Cuts at: 2171 2171
Size: 5515
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2550 928
Size: 971 651 3893
Fragments arranged by size:
3893 971 651
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3706 3740 1903
Size: 34 50 34 1685 34 3678
Fragments arranged by size:
3678 1685 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2120 2217 4126
Size: 107 122 71 631 605 448 97 1909
Cuts at: 4126 136
Size: 1525
Fragments arranged by size:
1909 1525 631 605 448 122 107 97
71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2286 2790 3576 4732 1790
Size: 496 504 786 1156 2573
Fragments arranged by size:
2573 1156 786 504 496
BmgBI CAC'GTC
Cuts at: 1318 2602 1318
Size: 1284 4231
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2539 3246 4204
Size: 416 53 83 399 24 1122 707 958
Cuts at: 4204 442
Size: 1753
Fragments arranged by size:
1753 1122 958 707 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2278 2297 2506 2654 2738 4175 1239
Size: 1039 19 209 148 84 1437 2579
Fragments arranged by size:
2579 1437 1039 209 148 84 19
Bpu10I CC'TnA_GC
Cuts at: 2096 2096
Size: 5515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2430 3640 4505 4803 2430
Size: 1210 865 298 3142
Fragments arranged by size:
3142 1210 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2120 4178 882
Size: 617 621 2058 2219
Fragments arranged by size:
2219 2058 621 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2168 3322 3704 4339
Size: 53 83 186 936 635 1154 382 635
Cuts at: 4339 275
Size: 1451
Fragments arranged by size:
1451 1154 936 635 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5290
Fragments arranged by size:
5290 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2554 2603 2606 1186
Size: 1368 49 3 4095
Fragments arranged by size:
4095 1368 49 3
BseYI C'CCAG_C
Cuts at: 2050 4718 5214 2050
Size: 2668 496 2351
Fragments arranged by size:
2668 2351 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 2591 1163
Size: 573 405 450 4087
Fragments arranged by size:
4087 573 450 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2235 2965 3726 4754 1540
Size: 275 186 234 730 761 1028 2301
Fragments arranged by size:
2301 1028 761 730 275 234 186
BsiHKAI G_wGCw'C
Cuts at: 2564 3576 3661 4732 2564
Size: 1012 85 1071 3347
Fragments arranged by size:
3347 1071 1012 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 2564 2790 2834 3576 3661 4732
Size: 399 496 278 226 44 742 85 1071
Cuts at: 4732 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 496 399 278 226 85
44
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 2814 2870 844
Size: 1352 618 56 3489
Fragments arranged by size:
3489 1352 618 56
BsrBI CCG'CTC
Cuts at: 1394 1823 3402 1394
Size: 429 1579 3507
Fragments arranged by size:
3507 1579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2207 2288 2309 2646 4159 4346 1700
Size: 110 397 81 21 337 1513 187 2869
Fragments arranged by size:
2869 1513 397 337 187 110 81 21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 2060 2062 2760 1925
Size: 135 2 698 4680
Fragments arranged by size:
4680 698 135 2
BssSI C'ACGA_G
Cuts at: 2326 3575 4587 2326
Size: 1249 1012 3254
Fragments arranged by size:
3254 1249 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 2149
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510
Size: 873 367 385 17 1452 86 12 346
Cuts at: 5510 1972
Size: 1977
Fragments arranged by size:
1977 1452 873 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2375 2375
Size: 5515
BtgI C'CryG_G
Cuts at: 513 1065 2343 513
Size: 552 1278 3685
Fragments arranged by size:
3685 1278 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 3031 3825 3845 3031
Size: 794 20 4701
Fragments arranged by size:
4701 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DraIII CAC_nnn'GTG
Cuts at: 2344 2344
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2209 2232
Size: 54 1425 50 275 18 291 88 23
Cuts at: 2232 2625 2962 3851 5480 8
Size: 393 337 889 1629 43
Fragments arranged by size:
1629 1425 889 393 337 291 275 88
54 50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 2232 2962 1537
Size: 275 420 730 4090
Fragments arranged by size:
4090 730 420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2228 3445 1353
Size: 875 1217 3423
Fragments arranged by size:
3423 1217 875
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 4476 4622 387
Size: 1064 138 2513 374 146 1280
Fragments arranged by size:
2513 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2278 2297 2506 2654 2738 3578 4175
Size: 393 646 19 209 148 84 840 597
Cuts at: 4175 4962 5230 1239
Size: 787 268 1524
Fragments arranged by size:
1524 840 787 646 597 393 268 209
148 84 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2099
Size: 5515
EcoO109I rG'GnC_Cy
Cuts at: 2045 2091 2218 2682 2786 2045
Size: 46 127 464 104 4774
Fragments arranged by size:
4774 464 127 104 46
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 5515
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 2057 2156 2171 4662 1678
Size: 379 99 15 2491 2531
Fragments arranged by size:
2531 2491 379 99 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373
Size: 32 135 32 2584 32 42 32 2626
Fragments arranged by size:
2626 2584 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KasI G'GCGC_C
Cuts at: 2167 2167
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2627 10
Size: 54 1425 343 795 2898
Fragments arranged by size:
2898 1425 795 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3473 3832 3991 518
Size: 648 2307 359 159 2042
Fragments arranged by size:
2307 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2074 2509 2931 3608 4756 5001 1606
Size: 411 57 435 422 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 435 422 411 245 57
NaeI GCC'GGC
Cuts at: 2209 2290 2209
Size: 81 5434
NarI GG'CG_CC
Cuts at: 2168 2168
Size: 5515
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4963
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NgoMIV G'CCGG_C
Cuts at: 2207 2288 2207
Size: 81 5434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2275 2873 4418 1132
Size: 97 1046 598 1545 2229
Fragments arranged by size:
2229 1545 1046 598 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 2529 1108
Size: 1236 185 4094
Fragments arranged by size:
4094 1236 185
PfoI T'CCnGG_A
Cuts at: 2349 2538 2655 2349
Size: 189 117 5209
Fragments arranged by size:
5209 189 117
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2045 2682 2045
Size: 637 4878
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 2394 3085 2394
Size: 691 4824
PspOMI G'GGCC_C
Cuts at: 2786 2786
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2616 2867 830
Size: 1786 251 3478
Fragments arranged by size:
3478 1786 251
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
PvuII CAG'CTG
Cuts at: 2017 2074 2509 2017
Size: 57 435 5023
Fragments arranged by size:
5023 435 57
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SanDI GG'GwC_CC
Cuts at: 2045 2045
Size: 5515
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2612 2863 3998 4679 4870 826
Size: 219 1567 251 1135 681 191 1471
Fragments arranged by size:
1567 1471 1135 681 251 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2217 2217
Size: 5515
SfoI GGC'GCC
Cuts at: 2169 2169
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2783 2842 1872
Size: 911 59 4545
Fragments arranged by size:
4545 911 59
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2445 3619 4520 4782 820
Size: 197 926 502 1174 901 262 1553
Fragments arranged by size:
1553 1174 926 901 502 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2275 2873 1132
Size: 1143 598 3774
Fragments arranged by size:
3774 1143 598
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 2579 1517
Size: 1062 4453
StyI C'CwwG_G
Cuts at: 513 1065 2954 513
Size: 552 1889 3074
Fragments arranged by size:
3074 1889 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 3559 3712 3897 4342 325
Size: 1255 1962 17 153 185 445 1498
Fragments arranged by size:
1962 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 2600 1316
Size: 1284 4231
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2781 2840 1870
Size: 911 59 4545
Fragments arranged by size:
4545 911 59
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SexAI SrfI SwaI XcmI