Restriction map for
pFN21AE0136
(Circular) (Six-base) MAPSORT of: pFN21AE0136.seq Check: 7403 from: 1 to: 5611
pFN21AE0136 5611 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2107 2107
Size: 5611
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2370 3421 278
Size: 53 83 186 936 834 1051 2468
Fragments arranged by size:
2468 1051 936 834 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2954 1937
Size: 1017 4594
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2840 3674 5058 5326 1632
Size: 1208 834 1384 268 1917
Fragments arranged by size:
1917 1384 1208 834 268
AfeI AGC'GCT
Cuts at: 2708 2708
Size: 5611
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5414
AflIII A'CryG_T
Cuts at: 1203 2346 4510 5487 1203
Size: 1143 2164 977 1327
Fragments arranged by size:
2164 1327 1143 977
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 2654 1166
Size: 1488 4123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5611
AlwNI CAG_nnn'CTG
Cuts at: 2089 2335 3354 4926 2089
Size: 246 1019 1572 2774
Fragments arranged by size:
2774 1572 1019 246
ApaI G_GGCC'C
Cuts at: 2053 2247 2053
Size: 194 5417
ApaLI G'TGCA_C
Cuts at: 3668 4824 3668
Size: 1156 4455
ApoI r'AATT_y
Cuts at: 1493 2920 3146 3298 5407 1493
Size: 1427 226 152 2109 1697
Fragments arranged by size:
2109 1697 1427 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2376 2936 1870
Size: 73 433 560 4545
Fragments arranged by size:
4545 560 433 73
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2932 4387 618
Size: 325 217 627 1145 1455 1842
Fragments arranged by size:
1842 1455 1145 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2053 2247 2930 1391
Size: 662 194 683 4072
Fragments arranged by size:
4072 683 662 194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4640
BbvCI CC'TCA_GC
Cuts at: 2892 2892
Size: 5611
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3802 3836 1903
Size: 34 50 34 1781 34 3678
Fragments arranged by size:
3678 1781 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2133 3503 4713 2133
Size: 1370 1210 3031
Fragments arranged by size:
3031 1370 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 2105 4222
Size: 107 122 71 631 605 376 57 2117
Cuts at: 4222 136
Size: 1525
Fragments arranged by size:
2117 1525 631 605 376 122 107 71
57
BglII A'GATC_T
Cuts at: 5606 5606
Size: 5611
BlpI GC'TnA_GC
Cuts at: 2825 3028 2825
Size: 203 5408
Bme1580I G_kGCm'C
Cuts at: 1790 2053 2247 2903 3672 4828 1790
Size: 263 194 656 769 1156 2573
Fragments arranged by size:
2573 1156 769 656 263 194
BmgBI CAC'GTC
Cuts at: 1318 2533 1318
Size: 1215 4396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2389 2635 3342
Size: 416 53 83 399 24 972 246 707
Cuts at: 3342 4300 442
Size: 958 1753
Fragments arranged by size:
1753 972 958 707 416 399 246 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 2618 4271 1239
Size: 1283 96 1653 2579
Fragments arranged by size:
2579 1653 1283 96
Bpu10I CC'TnA_GC
Cuts at: 2258 2892 2258
Size: 634 4977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3736 4601 4899 3736
Size: 865 298 4448
Fragments arranged by size:
4448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2512 4274 882
Size: 617 1013 1762 2219
Fragments arranged by size:
2219 1762 1013 617
BsaAI yAC'GTr
Cuts at: 493 1204 2347 3402 493
Size: 711 1143 1055 2702
Fragments arranged by size:
2702 1143 1055 711
BsaBI GATnn'nnATC
Cuts at: 5605 5605
Size: 5611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 3418 3800 4435
Size: 53 83 186 936 834 1051 382 635
Cuts at: 4435 275
Size: 1451
Fragments arranged by size:
1451 1051 936 834 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4044 4442 4716 4863 5274 4044
Size: 398 274 147 411 4381
Fragments arranged by size:
4381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5386
Fragments arranged by size:
5386 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 2786 2789 1186
Size: 829 771 3 4008
Fragments arranged by size:
4008 829 771 3
BseYI C'CCAG_C
Cuts at: 2082 2268 2272 2408 2423 2511 4814 5310 2082
Size: 186 4 136 15 88 2303 496 2383
Fragments arranged by size:
2383 2303 496 186 136 88 15 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2004 2429 2659 2681 2708 1163
Size: 573 268 425 230 22 27 4066
Fragments arranged by size:
4066 573 425 268 230 27 22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3061 3822 4850 1540
Size: 275 186 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3672 3757 4828 3672
Size: 85 1071 4455
Fragments arranged by size:
4455 1071 85
BsmI GAATG_Cn'
Cuts at: 3122 3215 3122
Size: 93 5518
BsmBI CGTCTCn'nnnn_
Cuts at: 2288 2288
Size: 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2247 2903 2930 3672 3757 4828
Size: 399 263 194 656 27 742 85 1071
Cuts at: 4828 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 656 399 263 194 85
27
BspEI T'CCGG_A
Cuts at: 5274 5274
Size: 5611
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2107 2966 844
Size: 1263 859 3489
Fragments arranged by size:
3489 1263 859
BsrBI CCG'CTC
Cuts at: 1394 1823 3498 1394
Size: 429 1675 3507
Fragments arranged by size:
3507 1675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2419 4106 4280 65
Size: 1183 1171 1687 174 1396
Fragments arranged by size:
1687 1396 1183 1171 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4255 4442 1700
Size: 110 2445 187 2869
Fragments arranged by size:
2869 2445 187 110
BsrGI T'GTAC_A
Cuts at: 96 2349 96
Size: 2253 3358
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5611
BssSI C'ACGA_G
Cuts at: 2752 3671 4683 2752
Size: 919 1012 3680
Fragments arranged by size:
3680 1012 919
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2673 794
Size: 1879 3732
BstBI TT'CG_AA
Cuts at: 4415 4415
Size: 5611
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5611
BstYI r'GATC_y
Cuts at: 1972 2941 3308 3693 3710 5162 5248 5260 5606
Size: 969 367 385 17 1452 86 12 346
Cuts at: 5606 1972
Size: 1977
Fragments arranged by size:
1977 1452 969 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2457 2457
Size: 5611
BtgI C'CryG_G
Cuts at: 513 1065 2805 513
Size: 552 1740 3319
Fragments arranged by size:
3319 1740 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4872
Fragments arranged by size:
4872 683 56
BtsI GCAGTG_nn'
Cuts at: 2671 2727 3127 3921 3941 2671
Size: 56 400 794 20 4341
Fragments arranged by size:
4341 794 400 56 20
ClaI AT'CG_AT
Cuts at: 1554 2227 1554
Size: 673 4938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2915 2915
Size: 5611
DraIII CAC_nnn'GTG
Cuts at: 2629 2629
Size: 5611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4618 809
Size: 507 3302 1802
Fragments arranged by size:
3302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2097 2106
Size: 54 1425 50 275 18 210 57 9
Cuts at: 2106 3058 3947 5576 8
Size: 952 889 1629 43
Fragments arranged by size:
1629 1425 952 889 275 210 57 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3058 1537
Size: 275 1246 4090
Fragments arranged by size:
4090 1246 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 2191 3541 1353
Size: 680 158 1350 3423
Fragments arranged by size:
3423 1350 680 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4198 4572 4718 387
Size: 1064 138 2609 374 146 1280
Fragments arranged by size:
2609 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 2618 2840 3674 4271 5058 5326
Size: 393 890 96 222 834 597 787 268
Cuts at: 5326 1239
Size: 1524
Fragments arranged by size:
1524 890 834 787 597 393 268 222
96
EcoO109I rG'GnC_Cy
Cuts at: 2049 2244 2332 2779 2049
Size: 195 88 447 4881
Fragments arranged by size:
4881 447 195 88
EcoRI G'AATT_C
Cuts at: 2920 3298 2920
Size: 378 5233
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 2199 2587 2710 4758 1678
Size: 521 388 123 2048 2531
Fragments arranged by size:
2531 2048 521 388 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2131 2163 4252 4284 4326
Size: 32 135 32 559 32 2089 32 42
Cuts at: 4326 4358 1373
Size: 32 2626
Fragments arranged by size:
2626 2089 559 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2955 3201 669
Size: 988 281 1017 246 3079
Fragments arranged by size:
3079 1017 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5611
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4725 4909 1305
Size: 3420 184 2007
Fragments arranged by size:
3420 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 2099 2108 10
Size: 54 1425 343 210 57 9 3513
Fragments arranged by size:
3513 1425 343 210 57 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2288 2654 3569 3928 4087 518
Size: 648 996 126 366 915 359 159 2042
Fragments arranged by size:
2042 996 915 648 366 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 2086 2272 2443 2896 3027 3704 4852 5097
Size: 480 186 171 453 131 677 1148 245
Cuts at: 5097 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 480 453 245 186 171
131
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5059
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2969 4514 1132
Size: 97 1740 1545 2229
Fragments arranged by size:
2229 1740 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5611
PciI A'CATG_T
Cuts at: 4510 4510
Size: 5611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5611
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 5193 5225 3680
Size: 32 1481 32 4066
Fragments arranged by size:
4066 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2332 2332
Size: 5611
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PspOMI G'GGCC_C
Cuts at: 2049 2243 2049
Size: 194 5417
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2471 2503 2471
Size: 32 5579
PstI C_TGCA'G
Cuts at: 830 2172 2963 830
Size: 1342 791 3478
Fragments arranged by size:
3478 1342 791
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
PvuII CAG'CTG
Cuts at: 2272 2896 2272
Size: 624 4987
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SbfI CC_TGCA'GG
Cuts at: 2963 2963
Size: 5611
ScaI AGT'ACT
Cuts at: 3859 5571 3859
Size: 1712 3899
SfcI C'TryA_G
Cuts at: 826 1045 2168 2248 2959 4094 4775 4966 826
Size: 219 1123 80 711 1135 681 191 1471
Fragments arranged by size:
1471 1135 1123 711 681 219 191 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 2048 2105 2048
Size: 57 5554
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5611
SmaI CCC'GGG
Cuts at: 1872 2378 2938 1872
Size: 506 560 4545
Fragments arranged by size:
4545 560 506
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3715 4616 4878 820
Size: 197 926 1772 901 262 1553
Fragments arranged by size:
1772 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2969 1132
Size: 1837 3774
SspI AAT'ATT
Cuts at: 5 52 3535 5
Size: 47 3483 2081
Fragments arranged by size:
3483 2081 47
StuI AGG'CCT
Cuts at: 1517 2057 1517
Size: 540 5071
StyI C'CwwG_G
Cuts at: 513 1065 2100 3050 513
Size: 552 1035 950 3074
Fragments arranged by size:
3074 1035 950 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2304 2617 2684 3638 3655 3808 3993
Size: 1255 724 313 67 954 17 153 185
Cuts at: 3993 4438 325
Size: 445 1498
Fragments arranged by size:
1498 1255 954 724 445 313 185 153
67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 2349 3857
Size: 274 80 33 51 1443 327 45 1508
Cuts at: 3857 5569 96
Size: 1712 138
Fragments arranged by size:
1712 1508 1443 327 274 138 80 51
45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2013 2045 2826 2858 5571 5603 2013
Size: 32 781 32 2713 32 2021
Fragments arranged by size:
2713 2021 781 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2687 1316
Size: 1371 4240
XbaI T'CTAG_A
Cuts at: 2947 2947
Size: 5611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2431 2584 2622 2431
Size: 153 38 5420
Fragments arranged by size:
5420 153 38
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5611
XmaI C'CCGG_G
Cuts at: 1870 2376 2936 1870
Size: 506 560 4545
Fragments arranged by size:
4545 560 506
XmnI GAAnn'nnTTC
Cuts at: 3740 3740
Size: 5611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2368 3419 276
Size: 53 83 186 936 834 1051 2468
Fragments arranged by size:
2468 1051 936 834 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI RsrII SacI SacII SanDI SapI SexAI
SfoI SrfI SwaI