Restriction map for
pFN21AE0135
(Circular) (Six-base) MAPSORT of: pFN21AE0135.seq Check: 8912 from: 1 to: 5722
pFN21AE0135 5722 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3065 1937
Size: 1128 4594
Acc65I G'GTAC_C
Cuts at: 3043 3043
Size: 5722
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2596 2701 3785 5169 5437 1632
Size: 964 105 1084 1384 268 1917
Fragments arranged by size:
1917 1384 1084 964 268 105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 4621 5598 1203
Size: 3418 977 1327
Fragments arranged by size:
3418 1327 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2687 2939 1184
Size: 1503 252 3967
Fragments arranged by size:
3967 1503 252
AlwNI CAG_nnn'CTG
Cuts at: 2619 2683 2973 3465 5037 2619
Size: 64 290 492 1572 3304
Fragments arranged by size:
3304 1572 492 290 64
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 3031 3257 3409 5518 1493
Size: 1538 226 152 2109 1697
Fragments arranged by size:
2109 1697 1538 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 2696 3047 1870
Size: 73 753 351 4545
Fragments arranged by size:
4545 753 351 73
BamHI G'GATC_C
Cuts at: 3052 3419 3052
Size: 367 5355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2634 3043 4498 618
Size: 325 217 627 234 613 409 1455 1842
Fragments arranged by size:
1842 1455 627 613 409 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2184 3041 1391
Size: 793 857 4072
Fragments arranged by size:
4072 857 793
BbeI G_GCGC'C
Cuts at: 2025 2025
Size: 5722
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4751
BbvCI CC'TCA_GC
Cuts at: 2726 2927 2726
Size: 201 5521
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3614 4824 3614
Size: 1210 4512
BclI T'GATC_A
Cuts at: 1548 2772 1548
Size: 1224 4498
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4333 136
Size: 107 122 71 631 605 2661 1525
Fragments arranged by size:
2661 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2352 5717 2352
Size: 3365 2357
BlpI GC'TnA_GC
Cuts at: 3139 3139
Size: 5722
Bme1580I G_kGCm'C
Cuts at: 1790 2637 3783 4939 1790
Size: 847 1146 1156 2573
Fragments arranged by size:
2573 1156 1146 847
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2480 2629 3453
Size: 416 53 83 399 24 1063 149 824
Cuts at: 3453 4411 442
Size: 958 1753
Fragments arranged by size:
1753 1063 958 824 416 399 149 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2341 2396 4382 1239
Size: 1088 14 55 1986 2579
Fragments arranged by size:
2579 1986 1088 55 14
Bpu10I CC'TnA_GC
Cuts at: 2726 2927 2726
Size: 201 5521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3847 4712 5010 2168
Size: 1679 865 298 2880
Fragments arranged by size:
2880 1679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2207 2508 4385 882
Size: 617 708 301 1877 2219
Fragments arranged by size:
2219 1877 708 617 301
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 2603 5716 2603
Size: 3113 2609
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 2883 3529 3911
Size: 53 83 186 936 489 861 646 382
Cuts at: 3911 4546 275
Size: 635 1451
Fragments arranged by size:
1451 936 861 646 635 489 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4155 4553 4827 4974 5385 4155
Size: 398 274 147 411 4492
Fragments arranged by size:
4492 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5497
Fragments arranged by size:
5497 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2393 2414 2423 2441 2444 2537 2540
Size: 943 264 21 9 18 3 93 3
Cuts at: 2540 2543 2609 2944 1186
Size: 3 66 335 3964
Fragments arranged by size:
3964 943 335 264 93 66 21 18
9 3 3 3
BseYI C'CCAG_C
Cuts at: 2973 4925 5421 2973
Size: 1952 496 3274
Fragments arranged by size:
3274 1952 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2041 3172 3933 4961 1540
Size: 275 186 40 1131 761 1028 2301
Fragments arranged by size:
2301 1131 1028 761 275 186 40
BsiHKAI G_wGCw'C
Cuts at: 2184 3783 3868 4939 2184
Size: 1599 85 1071 2967
Fragments arranged by size:
2967 1599 1071 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2637 3041 3783 3868 4939 1391
Size: 399 394 453 404 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 404 399 394 85
BspEI T'CCGG_A
Cuts at: 5385 5385
Size: 5722
BspHI T'CATG_A
Cuts at: 2124 2857 3611 5341 2124
Size: 733 754 1730 2505
Fragments arranged by size:
2505 1730 754 733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3077 844
Size: 2233 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2254 3609 1394
Size: 429 431 1355 3507
Fragments arranged by size:
3507 1355 431 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4217 4391 65
Size: 1183 2969 174 1396
Fragments arranged by size:
2969 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 2637 4366 4553 1700
Size: 110 226 601 1729 187 2869
Fragments arranged by size:
2869 1729 601 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 2754 96
Size: 2658 3064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 2204 3782 4794 2204
Size: 1578 1012 3132
Fragments arranged by size:
3132 1578 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2683 794
Size: 1889 3833
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2197
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2352 2700 3052 3419 3804 3821 5273 5359
Size: 380 348 352 367 385 17 1452 86
Cuts at: 5359 5371 5717 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 380 367 352 348 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2196 513
Size: 552 939 192 4039
Fragments arranged by size:
4039 939 552 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2092 487
Size: 683 56 866 4117
Fragments arranged by size:
4117 866 683 56
BtsI GCAGTG_nn'
Cuts at: 3238 4032 4052 3238
Size: 794 20 4908
Fragments arranged by size:
4908 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2807 4729 809
Size: 507 1491 1922 1802
Fragments arranged by size:
1922 1802 1491 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 2268 2415
Size: 54 1425 50 275 18 208 230 147
Cuts at: 2415 3169 4058 5687 8
Size: 754 889 1629 43
Fragments arranged by size:
1629 1425 889 754 275 230 208 147
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2038 3169 1537
Size: 275 226 1131 4090
Fragments arranged by size:
4090 1131 275 226
EarI CTCTTCn'nnn_
Cuts at: 1353 2382 2400 2439 2456 2541 2822 2846 3652
Size: 1029 18 39 17 85 281 24 806
Cuts at: 3652 1353
Size: 3423
Fragments arranged by size:
3423 1029 806 281 85 39 24 18
17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2656 4309 4683 4829 387
Size: 1064 138 1067 1653 374 146 1280
Fragments arranged by size:
1653 1280 1067 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2182 2182
Size: 5722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2341 2396 2596 2701 3785 4382
Size: 393 695 14 55 200 105 1084 597
Cuts at: 4382 5169 5437 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1084 787 695 597 393 268 200
105 55 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 2687 2378
Size: 309 5413
EcoRI G'AATT_C
Cuts at: 3031 3409 3031
Size: 378 5344
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2974 2785
Size: 189 5533
FseI GG_CCGG'CC
Cuts at: 2040 2040
Size: 5722
FspI TGC'GCA
Cuts at: 2157 4228 2157
Size: 2071 3651
HaeII r_GCGC'y
Cuts at: 1678 2025 2696 4869 1678
Size: 347 671 2173 2531
Fragments arranged by size:
2531 2173 671 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2714 2746 2764
Size: 32 135 32 599 32 511 32 18
Cuts at: 2764 2796 4363 4395 4437 4469 1373
Size: 32 1567 32 42 32 2626
Fragments arranged by size:
2626 1567 599 511 135 42 32 32
32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 2491 3066 3312 669
Size: 988 281 553 575 246 3079
Fragments arranged by size:
3079 988 575 553 281 246
HindIII A'AGCT_T
Cuts at: 748 2312 2497 748
Size: 1564 185 3973
Fragments arranged by size:
3973 1564 185
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KasI G'GCGC_C
Cuts at: 2021 2021
Size: 5722
KpnI G_GTAC'C
Cuts at: 3047 3047
Size: 5722
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2157 4836 5020 1305
Size: 852 2679 184 2007
Fragments arranged by size:
2679 2007 852 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 10
Size: 54 1425 343 438 3462
Fragments arranged by size:
3462 1425 438 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2862 3680 4039 4198 518
Size: 648 1696 818 359 159 2042
Fragments arranged by size:
2042 1696 818 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2266 2973 3138 3815 4963 5208 1606
Size: 660 707 165 677 1148 245 2120
Fragments arranged by size:
2120 1148 707 677 660 245 165
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 5722
NarI GG'CG_CC
Cuts at: 2022 2022
Size: 5722
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4231
Fragments arranged by size:
4231 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5722
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 3080 4625 1132
Size: 97 1851 1545 2229
Fragments arranged by size:
2229 1851 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 4621 4621
Size: 5722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2973 1108
Size: 1865 3857
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5304 5336 3791
Size: 32 1481 32 4177
Fragments arranged by size:
4177 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PstI C_TGCA'G
Cuts at: 830 2298 2688 3074 830
Size: 1468 390 386 3478
Fragments arranged by size:
3478 1468 390 386
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
PvuII CAG'CTG
Cuts at: 2266 2266
Size: 5722
SacI G_AGCT'C
Cuts at: 2184 2184
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SapI GCTCTTCn'nnn_
Cuts at: 2541 2541
Size: 5722
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SfcI C'TryA_G
Cuts at: 826 1045 2294 2684 3070 4205 4886 5077 826
Size: 219 1249 390 386 1135 681 191 1471
Fragments arranged by size:
1471 1249 1135 681 390 386 219 191
SfoI GGC'GCC
Cuts at: 2023 2023
Size: 5722
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 2637 1700
Size: 937 4785
SmaI CCC'GGG
Cuts at: 1872 3049 1872
Size: 1177 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 2696 3826 4727 4989 820
Size: 197 926 240 513 1130 901 262 1553
Fragments arranged by size:
1553 1130 926 901 513 262 240 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 3646 5
Size: 47 3594 2081
Fragments arranged by size:
3594 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5722
StyI C'CwwG_G
Cuts at: 513 1065 2004 2286 2814 3161 513
Size: 552 939 282 528 347 3074
Fragments arranged by size:
3074 939 552 528 347 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3749 3766 3919 4104 4549 325
Size: 1255 2169 17 153 185 445 1498
Fragments arranged by size:
2169 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 3009 3968
Size: 274 80 33 51 1443 777 255 959
Cuts at: 3968 5680 96
Size: 1712 138
Fragments arranged by size:
1712 1443 959 777 274 255 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5682 5714 5682
Size: 32 5690
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 2696 1943
Size: 753 4969
XmaI C'CCGG_G
Cuts at: 1870 3047 1870
Size: 1177 4545
XmnI GAAnn'nnTTC
Cuts at: 2714 3851 2714
Size: 1137 4585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI
SwaI XcmI