Restriction map for
pFN21AE0131
(Circular) (Six-base) MAPSORT of: pFN21AE0131.seq Check: 374 from: 1 to: 5254
pFN21AE0131 5254 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3064 278
Size: 53 83 186 936 1528 2468
Fragments arranged by size:
2468 1528 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2597 1937
Size: 660 4594
Acc65I G'GTAC_C
Cuts at: 2575 2575
Size: 5254
AclI AA'CG_TT
Cuts at: 1563 3381 3754 1563
Size: 1818 373 3063
Fragments arranged by size:
3063 1818 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2175 3317 4701 4969 1632
Size: 543 1142 1384 268 1917
Fragments arranged by size:
1917 1384 1142 543 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5057
AflIII A'CryG_T
Cuts at: 1203 4153 5130 1203
Size: 2950 977 1327
Fragments arranged by size:
2950 1327 977
AgeI A'CCGG_T
Cuts at: 4085 4085
Size: 5254
AhdI GACnn_n'nnGTC
Cuts at: 3983 3983
Size: 5254
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5254
AlwNI CAG_nnn'CTG
Cuts at: 2997 4569 2997
Size: 1572 3682
ApaLI G'TGCA_C
Cuts at: 3311 4467 3311
Size: 1156 4098
ApoI r'AATT_y
Cuts at: 1493 2563 2789 2941 5050 1493
Size: 1070 226 152 2109 1697
Fragments arranged by size:
2109 1697 1070 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3808 160
Size: 872 2776 1606
Fragments arranged by size:
2776 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5254
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 2579 1870
Size: 73 193 443 4545
Fragments arranged by size:
4545 443 193 73
BamHI G'GATC_C
Cuts at: 2584 2951 2584
Size: 367 4887
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 2468 2478 2575 4030
Size: 325 217 627 354 327 10 97 1455
Cuts at: 4030 618
Size: 1842
Fragments arranged by size:
1842 1455 627 354 327 325 217 97
10
BanII G_rGCy'C
Cuts at: 1391 2573 1391
Size: 1182 4072
BbeI G_GCGC'C
Cuts at: 2472 2482 2472
Size: 10 5244
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2425 928
Size: 971 526 3757
Fragments arranged by size:
3757 971 526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3445 3479 1903
Size: 34 1508 34 3678
Fragments arranged by size:
3678 1508 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3146 4356 3146
Size: 1210 4044
BclI T'GATC_A
Cuts at: 1548 2496 1548
Size: 948 4306
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3865 136
Size: 107 122 71 631 605 2193 1525
Fragments arranged by size:
2193 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5249 5249
Size: 5254
BlpI GC'TnA_GC
Cuts at: 2671 2671
Size: 5254
Bme1580I G_kGCm'C
Cuts at: 1790 3315 4471 1790
Size: 1525 1156 2573
Fragments arranged by size:
2573 1525 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5254
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2985 3943 442
Size: 416 53 83 399 24 1568 958 1753
Fragments arranged by size:
1753 1568 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 2420 2477 3914 1239
Size: 1043 138 57 1437 2579
Fragments arranged by size:
2579 1437 1043 138 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3379 4244 4542 3379
Size: 865 298 4091
Fragments arranged by size:
4091 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2483 3917 882
Size: 617 984 1434 2219
Fragments arranged by size:
2219 1434 984 617
BsaAI yAC'GTr
Cuts at: 493 1204 3045 493
Size: 711 1841 2702
Fragments arranged by size:
2702 1841 711
BsaBI GATnn'nnATC
Cuts at: 2292 5248 2292
Size: 2956 2298
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2074 2443 2469 2479
Size: 53 83 186 936 541 369 26 10
Cuts at: 2479 3061 3443 4078 275
Size: 582 382 635 1451
Fragments arranged by size:
1451 936 635 582 541 382 369 186
83 53 26 10
BsaWI w'CCGG_w
Cuts at: 3687 4085 4359 4506 4917 3687
Size: 398 274 147 411 4024
Fragments arranged by size:
4024 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5029
Fragments arranged by size:
5029 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5254
BseYI C'CCAG_C
Cuts at: 2244 4457 4953 2244
Size: 2213 496 2545
Fragments arranged by size:
2545 2213 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4681
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2704 3465 4493 1540
Size: 275 186 703 761 1028 2301
Fragments arranged by size:
2301 1028 761 703 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 3315 3400 4471 2097
Size: 1218 85 1071 2880
Fragments arranged by size:
2880 1218 1071 85
BsmI GAATG_Cn'
Cuts at: 2268 2765 2858 2268
Size: 497 93 4664
Fragments arranged by size:
4664 497 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2573 3315 3400 4471 1391
Size: 399 307 476 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 476 399 307 85
BspEI T'CCGG_A
Cuts at: 4917 4917
Size: 5254
BspHI T'CATG_A
Cuts at: 2412 3143 4873 2412
Size: 731 1730 2793
Fragments arranged by size:
2793 1730 731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2609 844
Size: 1765 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3141 1394
Size: 429 1318 3507
Fragments arranged by size:
3507 1318 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 3749 3923 65
Size: 1183 1275 1226 174 1396
Fragments arranged by size:
1396 1275 1226 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2439 3898 4085 1700
Size: 110 629 1459 187 2869
Fragments arranged by size:
2869 1459 629 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5254
BssHII G'CGCG_C
Cuts at: 1925 2449 1925
Size: 524 4730
BssSI C'ACGA_G
Cuts at: 3314 4326 3314
Size: 1012 4242
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5254
BstBI TT'CG_AA
Cuts at: 4058 4058
Size: 5254
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5254
BstXI CCAn_nnnn'nTGG
Cuts at: 2236 2236
Size: 5254
BstYI r'GATC_y
Cuts at: 1972 2126 2584 2951 3336 3353 4805 4891 4903
Size: 154 458 367 385 17 1452 86 12
Cuts at: 4903 5249 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 458 385 367 346 154 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2281 513
Size: 552 939 277 3486
Fragments arranged by size:
3486 939 552 277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2485 487
Size: 683 56 1259 3256
Fragments arranged by size:
3256 1259 683 56
BtsI GCAGTG_nn'
Cuts at: 2370 2770 3564 3584 2370
Size: 400 794 20 4040
Fragments arranged by size:
4040 794 400 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5254
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5219
DraI TTT'AAA
Cuts at: 2558 2558
Size: 5254
DraIII CAC_nnn'GTG
Cuts at: 2101 2101
Size: 5254
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4261 809
Size: 507 2945 1802
Fragments arranged by size:
2945 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2517 2701 3590
Size: 54 1425 50 275 18 687 184 889
Cuts at: 3590 5219 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 687 275 184 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2701 1537
Size: 275 889 4090
Fragments arranged by size:
4090 889 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 3184 1353
Size: 1026 805 3423
Fragments arranged by size:
3423 1026 805
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2048 3841 4215 4361 387
Size: 1064 138 459 1793 374 146 1280
Fragments arranged by size:
1793 1280 1064 459 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2175 2282 2420 2477 3317 3914 4701
Size: 393 543 107 138 57 840 597 787
Cuts at: 4701 4969 1239
Size: 268 1524
Fragments arranged by size:
1524 840 787 597 543 393 268 138
107 57
EcoO109I rG'GnC_Cy
Cuts at: 2015 2241 2015
Size: 226 5028
EcoRI G'AATT_C
Cuts at: 2563 2941 2563
Size: 378 4876
FspI TGC'GCA
Cuts at: 3760 3760
Size: 5254
HaeII r_GCGC'y
Cuts at: 1678 2472 2482 4401 1678
Size: 794 10 1919 2531
Fragments arranged by size:
2531 1919 794 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 3895 3927 3969
Size: 32 135 32 539 32 1752 32 42
Cuts at: 3969 4001 1373
Size: 32 2626
Fragments arranged by size:
2626 1752 539 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2437 2598 2844 669
Size: 988 281 499 161 246 3079
Fragments arranged by size:
3079 988 499 281 246 161
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5254
HpaI GTT'AAC
Cuts at: 2844 2844
Size: 5254
KasI G'GCGC_C
Cuts at: 2468 2478 2468
Size: 10 5244
KpnI G_GTAC'C
Cuts at: 2579 2579
Size: 5254
MfeI C'AATT_G
Cuts at: 2853 2853
Size: 5254
MluI A'CGCG_T
Cuts at: 5130 5130
Size: 5254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 4368 4552 1305
Size: 840 2223 184 2007
Fragments arranged by size:
2223 2007 840 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3432
Fragments arranged by size:
3432 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2286 2504 3212 3571 3730 518
Size: 648 1120 218 708 359 159 2042
Fragments arranged by size:
2042 1120 708 648 359 218 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2425 2670 3347 4495 4740 1606
Size: 642 177 245 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 245 245 177
NarI GG'CG_CC
Cuts at: 2469 2479 2469
Size: 10 5244
NcoI C'CATG_G
Cuts at: 513 1065 2004 2281 513
Size: 552 939 277 3486
Fragments arranged by size:
3486 939 552 277
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5254
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5254
NotI GC'GGCC_GC
Cuts at: 2701 2701
Size: 5254
NspI r_CATG'y
Cuts at: 1132 1229 2612 4157 1132
Size: 97 1383 1545 2229
Fragments arranged by size:
2229 1545 1383 97
PasI CC'CwG_GG
Cuts at: 1348 2351 1348
Size: 1003 4251
PciI A'CATG_T
Cuts at: 4153 4153
Size: 5254
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2535 1108
Size: 1427 3827
PfoI T'CCnGG_A
Cuts at: 2298 2298
Size: 5254
PmeI GTTT'AAAC
Cuts at: 2558 2558
Size: 5254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 4836 4868 3323
Size: 32 1481 32 3709
Fragments arranged by size:
3709 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2241 2241
Size: 5254
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5254
PsiI TTA'TAA
Cuts at: 2824 2824
Size: 5254
PstI C_TGCA'G
Cuts at: 830 2606 830
Size: 1776 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5254
PvuII CAG'CTG
Cuts at: 2248 2425 2248
Size: 177 5077
SalI G'TCGA_C
Cuts at: 1936 2596 1936
Size: 660 4594
SanDI GG'GwC_CC
Cuts at: 2241 2241
Size: 5254
SbfI CC_TGCA'GG
Cuts at: 2606 2606
Size: 5254
ScaI AGT'ACT
Cuts at: 3502 5214 3502
Size: 1712 3542
SexAI A'CCwGG_T
Cuts at: 2097 2550 2097
Size: 453 4801
SfcI C'TryA_G
Cuts at: 826 1045 2602 3737 4418 4609 826
Size: 219 1557 1135 681 191 1471
Fragments arranged by size:
1557 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2470 2480 2470
Size: 10 5244
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5254
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5254
SmaI CCC'GGG
Cuts at: 1872 2138 2581 1872
Size: 266 443 4545
Fragments arranged by size:
4545 443 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3358 4259 4521 820
Size: 197 926 1415 901 262 1553
Fragments arranged by size:
1553 1415 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3045 493
Size: 2552 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5254
SphI G_CATG'C
Cuts at: 1132 2612 1132
Size: 1480 3774
SspI AAT'ATT
Cuts at: 5 52 3178 5
Size: 47 3126 2081
Fragments arranged by size:
3126 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5254
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2077 2281 2320 2693 513
Size: 552 939 24 49 204 39 373 3074
Fragments arranged by size:
3074 939 552 373 204 49 39 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2121 3281 3298 3451 3636 4081 325
Size: 1255 541 1160 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1160 541 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2395 3500 5212
Size: 274 80 33 51 1443 418 1105 1712
Cuts at: 5212 96
Size: 138
Fragments arranged by size:
1712 1443 1105 418 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5214 5246 5214
Size: 32 5222
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5254
XbaI T'CTAG_A
Cuts at: 2590 2590
Size: 5254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2035 2306 2035
Size: 271 4983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5254
XmaI C'CCGG_G
Cuts at: 1870 2136 2579 1870
Size: 266 443 4545
Fragments arranged by size:
4545 443 266
XmnI GAAnn'nnTTC
Cuts at: 3383 3383
Size: 5254
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3062 276
Size: 53 83 186 936 1528 2468
Fragments arranged by size:
2468 1528 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SfiI SrfI SwaI