Restriction map for
pFN21AE0130
(Circular) (Six-base) MAPSORT of: pFN21AE0130.seq Check: 4014 from: 1 to: 5902
pFN21AE0130 5902 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3245 1937
Size: 1308 4594
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 3201 4029 4402 1563
Size: 1638 828 373 3063
Fragments arranged by size:
3063 1638 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3965 5349 5617 1632
Size: 2333 1384 268 1917
Fragments arranged by size:
2333 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 2710 4631 2710
Size: 1921 3981
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2081 1184
Size: 897 5005
AlwNI CAG_nnn'CTG
Cuts at: 2467 3645 5217 2467
Size: 1178 1572 3152
Fragments arranged by size:
3152 1572 1178
ApaI G_GGCC'C
Cuts at: 2267 2884 2267
Size: 617 5285
ApaLI G'TGCA_C
Cuts at: 2630 3959 5115 2630
Size: 1329 1156 3417
Fragments arranged by size:
3417 1329 1156
ApoI r'AATT_y
Cuts at: 1493 2105 3211 3437 3589 5698 1493
Size: 612 1106 226 152 2109 1697
Fragments arranged by size:
2109 1697 1106 612 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 3042 3227 1870
Size: 73 177 922 185 4545
Fragments arranged by size:
4545 922 185 177 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 2375 2457 3038 3050
Size: 325 217 627 244 344 82 581 12
Cuts at: 3050 3223 4678 618
Size: 173 1455 1842
Fragments arranged by size:
1842 1455 627 581 344 325 244 217
173 82 12
BanII G_rGCy'C
Cuts at: 1391 2211 2267 2884 2967 3019 3159 3174 3221
Size: 820 56 617 83 52 140 15 47
Cuts at: 3221 1391
Size: 4072
Fragments arranged by size:
4072 820 617 140 83 56 52 47
15
BbeI G_GCGC'C
Cuts at: 2379 2379
Size: 5902
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4931
BbvCI CC'TCA_GC
Cuts at: 3150 3150
Size: 5902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2195 2663 4513
Size: 107 122 71 631 605 523 468 1850
Cuts at: 4513 136
Size: 1525
Fragments arranged by size:
1850 1525 631 605 523 468 122 107
71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 2748 3319 2748
Size: 571 5331
Bme1580I G_kGCm'C
Cuts at: 1790 2267 2289 2634 2884 3043 3055 3963 5119
Size: 477 22 345 250 159 12 908 1156
Cuts at: 5119 1790
Size: 2573
Fragments arranged by size:
2573 1156 908 477 345 250 159 22
12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2268 3633 4591
Size: 416 53 83 399 24 851 1365 958
Cuts at: 4591 442
Size: 1753
Fragments arranged by size:
1753 1365 958 851 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3113 3146 3206 4562 1239
Size: 1874 33 60 1356 2579
Fragments arranged by size:
2579 1874 1356 60 33
Bpu10I CC'TnA_GC
Cuts at: 2296 3150 2296
Size: 854 5048
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3158 4027 4892 5190 3158
Size: 869 865 298 3870
Fragments arranged by size:
3870 869 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2321 2572 2943 4565 882
Size: 617 822 251 371 1622 2219
Fragments arranged by size:
2219 1622 822 617 371 251
BsaAI yAC'GTr
Cuts at: 493 1204 3693 493
Size: 711 2489 2702
Fragments arranged by size:
2702 2489 711
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2376 3709 4091 4726
Size: 53 83 186 936 843 1333 382 635
Cuts at: 4726 275
Size: 1451
Fragments arranged by size:
1451 1333 936 843 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4335 4733 5007 5154 5565 4335
Size: 398 274 147 411 4672
Fragments arranged by size:
4672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5677
Fragments arranged by size:
5677 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2318 2522 2596 2599 2602 3116 3149
Size: 1006 126 204 74 3 3 514 33
Cuts at: 3149 3167 1186
Size: 18 3921
Fragments arranged by size:
3921 1006 514 204 126 74 33 18
3 3
BseYI C'CCAG_C
Cuts at: 2959 5105 5601 2959
Size: 2146 496 3260
Fragments arranged by size:
3260 2146 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2215 2323 2851 3352 4113 5141
Size: 275 186 214 108 528 501 761 1028
Cuts at: 5141 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 528 501 275 214 186
108
BsiHKAI G_wGCw'C
Cuts at: 2211 2634 3159 3963 4048 5119 2211
Size: 423 525 804 85 1071 2994
Fragments arranged by size:
2994 1071 804 525 423 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
BsmBI CGTCTCn'nnnn_
Cuts at: 2774 2774
Size: 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2267 2289 2634 2884 2967 3019
Size: 399 421 56 22 345 250 83 52
Cuts at: 3019 3043 3055 3159 3174 3221 3963 4048 5119
Size: 24 12 104 15 47 742 85 1071
Cuts at: 5119 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 421 399 345 250 104
85 83 56 52 47 24 22 15
12
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3257 844
Size: 2413 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2747 3789 1394
Size: 429 924 1042 3507
Fragments arranged by size:
3507 1042 924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2034 2372 3035 4546 4733 1700
Size: 110 224 338 663 1511 187 2869
Fragments arranged by size:
2869 1511 663 338 224 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 2627 3962 4974 2627
Size: 1335 1012 3555
Fragments arranged by size:
3555 1335 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2403 2629 794
Size: 1609 226 4067
Fragments arranged by size:
4067 1609 226
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 2011 1184
Size: 827 5075
BstXI CCAn_nnnn'nTGG
Cuts at: 2699 2699
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 3232 3599 3984 4001 5453 5539 5551 5897
Size: 1260 367 385 17 1452 86 12 346
Cuts at: 5897 1972
Size: 1977
Fragments arranged by size:
1977 1452 1260 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2132 2861 2132
Size: 729 5173
BtgI C'CryG_G
Cuts at: 513 1065 2004 2193 2267 2814 513
Size: 552 939 189 74 547 3601
Fragments arranged by size:
3601 939 552 547 189 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 3418 4212 4232 3418
Size: 794 20 5088
Fragments arranged by size:
5088 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2219 4909 809
Size: 507 903 2690 1802
Fragments arranged by size:
2690 1802 903 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 2832 2928
Size: 54 1425 50 275 18 540 462 96
Cuts at: 2928 3349 4238 5867 8
Size: 421 889 1629 43
Fragments arranged by size:
1629 1425 889 540 462 421 275 96
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3832 1353
Size: 2479 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2660 2833 4489 4863 5009 387
Size: 1064 138 1071 173 1656 374 146 1280
Fragments arranged by size:
1656 1280 1071 1064 374 173 146 138
EcoICRI GAG'CTC
Cuts at: 2209 3157 2209
Size: 948 4954
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3113 3146 3206 3965 4562 5349 5617
Size: 393 1481 33 60 759 597 787 268
Cuts at: 5617 1239
Size: 1524
Fragments arranged by size:
1524 1481 787 759 597 393 268 60
33
EcoNI CCTnn'n_nnAGG
Cuts at: 2690 3128 3164 2690
Size: 438 36 5428
Fragments arranged by size:
5428 438 36
EcoO109I rG'GnC_Cy
Cuts at: 2062 2263 2827 2880 3101 3121 2062
Size: 201 564 53 221 20 4843
Fragments arranged by size:
4843 564 221 201 53 20
EcoRI G'AATT_C
Cuts at: 2105 3211 3589 2105
Size: 1106 378 4418
Fragments arranged by size:
4418 1106 378
FspI TGC'GCA
Cuts at: 2396 4408 2396
Size: 2012 3890
HaeII r_GCGC'y
Cuts at: 1678 2379 5049 1678
Size: 701 2670 2531
Fragments arranged by size:
2670 2531 701
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2696 2728 4543 4575 4617
Size: 32 135 32 1124 32 1815 32 42
Cuts at: 4617 4649 1373
Size: 32 2626
Fragments arranged by size:
2626 1815 1124 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2050 2224 3246 3492 669
Size: 988 281 112 174 1022 246 3079
Fragments arranged by size:
3079 1022 988 281 246 174 112
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KasI G'GCGC_C
Cuts at: 2375 2375
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5016 5200 1305
Size: 3711 184 2007
Fragments arranged by size:
3711 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2834 10
Size: 54 1425 343 1002 3078
Fragments arranged by size:
3078 1425 1002 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2543 2729 3860 4219 4378 518
Size: 648 843 534 186 1131 359 159 2042
Fragments arranged by size:
2042 1131 843 648 534 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 2195 3318 3995 5143 5388 1606
Size: 589 1123 677 1148 245 2120
Fragments arranged by size:
2120 1148 1123 677 589 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 5902
NarI GG'CG_CC
Cuts at: 2376 2376
Size: 5902
NcoI C'CATG_G
Cuts at: 513 1065 2004 2814 513
Size: 552 939 810 3601
Fragments arranged by size:
3601 939 810 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 2555 3260 4805 1132
Size: 97 1326 705 1545 2229
Fragments arranged by size:
2229 1545 1326 705 97
PasI CC'CwG_GG
Cuts at: 1348 2241 2380 2439 2501 1348
Size: 893 139 59 62 4749
Fragments arranged by size:
4749 893 139 62 59
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2467 1108
Size: 1359 4543
PfoI T'CCnGG_A
Cuts at: 2082 2337 2480 3162 3183 2082
Size: 255 143 682 21 4801
Fragments arranged by size:
4801 682 255 143 21
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3101 3101
Size: 5902
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PspOMI G'GGCC_C
Cuts at: 2263 2880 2263
Size: 617 5285
PspXI vC'TCGA_Gb
Cuts at: 2120 2120
Size: 5902
PstI C_TGCA'G
Cuts at: 830 2025 2625 2808 3254 830
Size: 1195 600 183 446 3478
Fragments arranged by size:
3478 1195 600 446 183
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
SacI G_AGCT'C
Cuts at: 2211 3159 2211
Size: 948 4954
SacII CC_GC'GG
Cuts at: 2196 2196
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2021 2621 2804 3250 4385 5066 5257
Size: 219 976 600 183 446 1135 681 191
Cuts at: 5257 826
Size: 1471
Fragments arranged by size:
1471 1135 976 681 600 446 219 191
183
SfoI GGC'GCC
Cuts at: 2377 2377
Size: 5902
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3044 3229 1872
Size: 1172 185 4545
Fragments arranged by size:
4545 1172 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 3173 4006 4907 5169 820
Size: 197 926 177 1053 833 901 262 1553
Fragments arranged by size:
1553 1053 926 901 833 262 197 177
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SrfI GCCC'GGGC
Cuts at: 3044 3044
Size: 5902
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 2137 1517
Size: 620 5282
StyI C'CwwG_G
Cuts at: 513 1065 2004 2814 3341 513
Size: 552 939 810 527 3074
Fragments arranged by size:
3074 939 810 552 527
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 2309 2372 2411 2431 3929 3946
Size: 1255 723 6 63 39 20 1498 17
Cuts at: 3946 4099 4284 4729 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1498 1255 723 445 185 153 63
39 20 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4148 5860 96
Size: 274 80 33 51 1443 2171 1712 138
Fragments arranged by size:
2171 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 2197
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 2120 1943
Size: 177 5725
XmaI C'CCGG_G
Cuts at: 1870 3042 3227 1870
Size: 1172 185 4545
Fragments arranged by size:
4545 1172 185
XmnI GAAnn'nnTTC
Cuts at: 4031 4031
Size: 5902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PsrI PvuII RsrII SanDI SapI SexAI
SfiI SwaI