Restriction map for
pFN21AE0129
(Circular) (Six-base) MAPSORT of: pFN21AE0129.seq Check: 488 from: 1 to: 5449
pFN21AE0129 5449 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2105 2274 2650 2105
Size: 169 376 4904
Fragments arranged by size:
4904 376 169
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2610 2792 1937
Size: 673 182 4594
Fragments arranged by size:
4594 673 182
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3512 4896 5164 1632
Size: 1880 1384 268 1917
Fragments arranged by size:
1917 1880 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 4348 5325 1203
Size: 3145 977 1327
Fragments arranged by size:
3145 1327 977
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5449
AlwNI CAG_nnn'CTG
Cuts at: 2266 3192 4764 2266
Size: 926 1572 2951
Fragments arranged by size:
2951 1572 926
ApaI G_GGCC'C
Cuts at: 2051 2521 2051
Size: 470 4979
ApaLI G'TGCA_C
Cuts at: 2081 3506 4662 2081
Size: 1425 1156 2868
Fragments arranged by size:
2868 1425 1156
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2774 1870
Size: 73 831 4545
Fragments arranged by size:
4545 831 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2417 2450 2417
Size: 33 5416
BamHI G'GATC_C
Cuts at: 2779 3146 2779
Size: 367 5082
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2623 2770 4225 618
Size: 325 217 627 278 558 147 1455 1842
Fragments arranged by size:
1842 1455 627 558 325 278 217 147
BanII G_rGCy'C
Cuts at: 1391 2051 2325 2521 2768 1391
Size: 660 274 196 247 4072
Fragments arranged by size:
4072 660 274 247 196
BbeI G_GCGC'C
Cuts at: 2069 2069
Size: 5449
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2220 928
Size: 971 321 4157
Fragments arranged by size:
4157 971 321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3640 3674 1903
Size: 34 1703 34 3678
Fragments arranged by size:
3678 1703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2656 4060 136
Size: 107 122 71 631 605 984 1404 1525
Fragments arranged by size:
1525 1404 984 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5444 5444
Size: 5449
BlpI GC'TnA_GC
Cuts at: 2866 2866
Size: 5449
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2085 2521 2628 3510 4666 1790
Size: 261 34 436 107 882 1156 2573
Fragments arranged by size:
2573 1156 882 436 261 107 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5449
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2444 4109 1239
Size: 1205 1665 2579
Fragments arranged by size:
2579 1665 1205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3574 4439 4737 3574
Size: 865 298 4286
Fragments arranged by size:
4286 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2180 2416 4112 882
Size: 617 681 236 1696 2219
Fragments arranged by size:
2219 1696 681 617 236
BsaAI yAC'GTr
Cuts at: 493 1204 3240 493
Size: 711 2036 2702
Fragments arranged by size:
2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2199 3256 3638
Size: 53 83 186 936 533 133 1057 382
Cuts at: 3638 4273 275
Size: 635 1451
Fragments arranged by size:
1451 1057 936 635 533 382 186 133
83 53
BsaWI w'CCGG_w
Cuts at: 3882 4280 4554 4701 5112 3882
Size: 398 274 147 411 4219
Fragments arranged by size:
4219 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2607 2637 1178
Size: 30 165 30 1204 30 3990
Fragments arranged by size:
3990 1204 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5449
BseYI C'CCAG_C
Cuts at: 2568 4652 5148 2568
Size: 2084 496 2869
Fragments arranged by size:
2869 2084 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2390 1163
Size: 573 654 4222
Fragments arranged by size:
4222 654 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2899 3660 4688 1540
Size: 275 186 898 761 1028 2301
Fragments arranged by size:
2301 1028 898 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2592 3510 3595 4666 2085
Size: 507 918 85 1071 2868
Fragments arranged by size:
2868 1071 918 507 85
BsmI GAATG_Cn'
Cuts at: 2960 3053 2960
Size: 93 5356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2085 2325 2521 2592 2628 2768
Size: 399 261 34 240 196 71 36 140
Cuts at: 2768 3510 3595 4666 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 261 240 196 140
85 71 36 34
BspEI T'CCGG_A
Cuts at: 5112 5112
Size: 5449
BspHI T'CATG_A
Cuts at: 3338 5068 3338
Size: 1730 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2105 2274 2445 2596 2650 2804 844
Size: 1261 169 171 151 54 154 3489
Fragments arranged by size:
3489 1261 171 169 154 151 54
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2145 3944 4118 65
Size: 1183 897 1799 174 1396
Fragments arranged by size:
1799 1396 1183 897 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 2243 4093 4280 1700
Size: 110 252 181 1850 187 2869
Fragments arranged by size:
2869 1850 252 187 181 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 2274 3509 4521 2274
Size: 1235 1012 3202
Fragments arranged by size:
3202 1235 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 794
Size: 1472 3977
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2742 2779 3146 3531 3548 5000 5086 5098
Size: 770 37 367 385 17 1452 86 12
Cuts at: 5098 5444 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 770 385 367 346 86 37
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2539 2580 2651 2730 513
Size: 552 939 535 41 71 79 3232
Fragments arranged by size:
3232 939 552 535 79 71 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2560 487
Size: 683 56 1334 3376
Fragments arranged by size:
3376 1334 683 56
BtsI GCAGTG_nn'
Cuts at: 2151 2378 2965 3759 3779 2151
Size: 227 587 794 20 3821
Fragments arranged by size:
3821 794 587 227 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2753 2753
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2896 3785
Size: 54 1425 50 275 18 411 655 889
Cuts at: 3785 5414 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 655 411 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 1537
Size: 275 1084 4090
Fragments arranged by size:
4090 1084 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 3379 1353
Size: 897 1129 3423
Fragments arranged by size:
3423 1129 897
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2040 4036 4410 4556 387
Size: 1064 138 451 1996 374 146 1280
Fragments arranged by size:
1996 1280 1064 451 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 3512 4109 4896 5164 1239
Size: 393 812 1068 597 787 268 1524
Fragments arranged by size:
1524 1068 812 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2033 2047 2518 2033
Size: 14 471 4964
Fragments arranged by size:
4964 471 14
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 2069 2549 4596 1678
Size: 391 480 2047 2531
Fragments arranged by size:
2531 2047 480 391
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4090 4122 4164 4196 1373
Size: 32 135 32 2518 32 42 32 2626
Fragments arranged by size:
2626 2518 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 3039 669
Size: 988 281 855 246 3079
Fragments arranged by size:
3079 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KasI G'GCGC_C
Cuts at: 2065 2065
Size: 5449
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2319 4563 4747 1305
Size: 1014 2244 184 2007
Fragments arranged by size:
2244 2007 1014 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3627
Fragments arranged by size:
3627 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2579 3407 3766 3925 518
Size: 648 1122 291 828 359 159 2042
Fragments arranged by size:
2042 1122 828 648 359 291 159
MspA1I CmG'CkG
Cuts at: 1606 2484 2491 2865 3542 4690 4935 1606
Size: 878 7 374 677 1148 245 2120
Fragments arranged by size:
2120 1148 878 677 374 245 7
NaeI GCC'GGC
Cuts at: 2064 2245 2064
Size: 181 5268
NarI GG'CG_CC
Cuts at: 2066 2066
Size: 5449
NcoI C'CATG_G
Cuts at: 513 1065 2004 2539 2730 513
Size: 552 939 535 191 3232
Fragments arranged by size:
3232 939 552 535 191
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5449
NgoMIV G'CCGG_C
Cuts at: 2062 2243 2062
Size: 181 5268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2807 4352 1132
Size: 97 1578 1545 2229
Fragments arranged by size:
2229 1578 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2449 1108
Size: 1341 4108
PfoI T'CCnGG_A
Cuts at: 2355 2355
Size: 5449
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PspOMI G'GGCC_C
Cuts at: 2047 2517 2047
Size: 470 4979
PstI C_TGCA'G
Cuts at: 830 2041 2607 2801 830
Size: 1211 566 194 3478
Fragments arranged by size:
3478 1211 566 194
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
PvuII CAG'CTG
Cuts at: 2484 2484
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SapI GCTCTTCn'nnn_
Cuts at: 2250 2250
Size: 5449
SbfI CC_TGCA'GG
Cuts at: 2607 2801 2607
Size: 194 5255
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SexAI A'CCwGG_T
Cuts at: 2448 2448
Size: 5449
SfcI C'TryA_G
Cuts at: 826 1045 2037 2603 2797 3932 4613 4804 826
Size: 219 992 566 194 1135 681 191 1471
Fragments arranged by size:
1471 1135 992 681 566 219 194 191
SfoI GGC'GCC
Cuts at: 2067 2067
Size: 5449
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2776 1872
Size: 904 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3553 4454 4716 820
Size: 197 926 1610 901 262 1553
Fragments arranged by size:
1610 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2807 1132
Size: 1675 3774
SspI AAT'ATT
Cuts at: 5 52 2701 3373 5
Size: 47 2649 672 2081
Fragments arranged by size:
2649 2081 672 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5449
StyI C'CwwG_G
Cuts at: 513 1065 2004 2539 2730 2888 513
Size: 552 939 535 191 158 3074
Fragments arranged by size:
3074 939 552 535 191 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 3493 3646 3831 4276 325
Size: 1255 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3695 5407 96
Size: 274 80 33 51 1443 1718 1712 138
Fragments arranged by size:
1718 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2607 2639 5409 5441 2607
Size: 32 2770 32 2615
Fragments arranged by size:
2770 2615 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2774 1870
Size: 904 4545
XmnI GAAnn'nnTTC
Cuts at: 3578 3578
Size: 5449
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SrfI SwaI XcmI