Restriction map for
pFN21AE0127
(Circular) (Six-base) MAPSORT of: pFN21AE0127.seq Check: 4734 from: 1 to: 5833
pFN21AE0127 5833 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 2010 3154 2010
Size: 1144 4689
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2027 2075 2555 2661 3896 5280 5548 1632
Size: 395 48 480 106 1235 1384 268 1917
Fragments arranged by size:
1917 1384 1235 480 395 268 106 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 2331 3084 4562 2331
Size: 753 1478 3602
Fragments arranged by size:
3602 1478 753
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2828 1184
Size: 1644 4189
AlwNI CAG_nnn'CTG
Cuts at: 3576 5148 3576
Size: 1572 4261
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 2867 3142 3368 3520 5629 1493
Size: 1374 275 226 152 2109 1697
Fragments arranged by size:
2109 1697 1374 275 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2010 2127 2857 3154 4609
Size: 325 217 627 223 117 730 297 1455
Cuts at: 4609 618
Size: 1842
Fragments arranged by size:
1842 1455 730 627 325 297 223 217
117
BanII G_rGCy'C
Cuts at: 1391 2757 3152 1391
Size: 1366 395 4072
Fragments arranged by size:
4072 1366 395
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4862
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2223 2257 4024 4058 1903
Size: 34 286 34 1767 34 3678
Fragments arranged by size:
3678 1767 286 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2284 3725 4935 2284
Size: 1441 1210 3182
Fragments arranged by size:
3182 1441 1210
BclI T'GATC_A
Cuts at: 1548 2994 1548
Size: 1446 4387
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 2248 3250 2248
Size: 1002 4831
Bme1580I G_kGCm'C
Cuts at: 1790 3894 5050 1790
Size: 2104 1156 2573
Fragments arranged by size:
2573 2104 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2717 1056
Size: 1661 4172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2671 4493 1239
Size: 1432 1822 2579
Fragments arranged by size:
2579 1822 1432
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2583 3958 4823 5121 2583
Size: 1375 865 298 3295
Fragments arranged by size:
3295 1375 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2229 2336 4496 882
Size: 617 730 107 2160 2219
Fragments arranged by size:
2219 2160 730 617 107
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3640 4022 4657 275
Size: 53 83 186 936 2107 382 635 1451
Fragments arranged by size:
2107 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5608
Fragments arranged by size:
5608 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2076 2867 3034 1186
Size: 890 791 167 3985
Fragments arranged by size:
3985 890 791 167
BseYI C'CCAG_C
Cuts at: 5036 5532 5036
Size: 496 5337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2607 3894 3979 5050 2607
Size: 1287 85 1071 3390
Fragments arranged by size:
3390 1287 1071 85
BsmI GAATG_Cn'
Cuts at: 2467 2573 2763 3344 3437 2467
Size: 106 190 581 93 4863
Fragments arranged by size:
4863 581 190 106 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3100 3100
Size: 5833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2607 2757 3152 3894 3979 5050 1391
Size: 399 817 150 395 742 85 1071 2174
Fragments arranged by size:
2174 1071 817 742 399 395 150 85
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3188 844
Size: 2344 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2247 3720 1394
Size: 429 424 1473 3507
Fragments arranged by size:
3507 1473 429 424
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5833
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstXI CCAn_nnnn'nTGG
Cuts at: 2335 2942 2335
Size: 607 5226
BstYI r'GATC_y
Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828
Size: 1191 367 385 17 1452 86 12 346
Cuts at: 5828 1972
Size: 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2862 2862
Size: 5833
BtgI C'CryG_G
Cuts at: 513 1065 2972 513
Size: 552 1907 3374
Fragments arranged by size:
3374 1907 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 2239 2697 2810 3041 3349 4143 4163 2239
Size: 458 113 231 308 794 20 3909
Fragments arranged by size:
3909 794 458 308 231 113 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2363 3280 4169
Size: 54 1425 50 275 18 533 917 889
Cuts at: 4169 5798 8
Size: 1629 43
Fragments arranged by size:
1629 1425 917 889 533 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3763 1353
Size: 2410 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2075 2555 2661 2671 3896 4493
Size: 393 395 48 480 106 10 1225 597
Cuts at: 4493 5280 5548 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1225 787 597 480 395 393 268
106 48 10
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 4980 1678
Size: 3302 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2317 2349 3070 3102 4474
Size: 32 135 32 745 32 721 32 1372
Cuts at: 4474 4506 4548 4580 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1372 745 721 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3028 3177 3423 669
Size: 988 281 1090 149 246 3079
Fragments arranged by size:
3079 1090 988 281 246 149
HindIII A'AGCT_T
Cuts at: 748 2101 748
Size: 1353 4480
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 2014 3158 2014
Size: 1144 4689
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2899 4947 5131 1305
Size: 1594 2048 184 2007
Fragments arranged by size:
2048 2007 1594 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2365 10
Size: 54 1425 343 533 3478
Fragments arranged by size:
3478 1425 533 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2768 3052 3791 4150 4309 518
Size: 648 1602 284 739 359 159 2042
Fragments arranged by size:
2042 1602 739 648 359 284 159
MspA1I CmG'CkG
Cuts at: 1606 3249 3926 5074 5319 1606
Size: 1643 677 1148 245 2120
Fragments arranged by size:
2120 1643 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2972 513
Size: 552 1907 3374
Fragments arranged by size:
3374 1907 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 2713 1052
Size: 1661 4172
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 3191 4736 1132
Size: 97 1962 1545 2229
Fragments arranged by size:
2229 1962 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PstI C_TGCA'G
Cuts at: 830 3185 830
Size: 2355 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 3181 4316 4997 5188 826
Size: 219 2136 1135 681 191 1471
Fragments arranged by size:
2136 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2598 3937 4838 5100 820
Size: 197 926 655 1339 901 262 1553
Fragments arranged by size:
1553 1339 926 901 655 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3075 3757 5
Size: 47 3023 682 2081
Fragments arranged by size:
3023 2081 682 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2972 3272 513
Size: 552 1907 300 3074
Fragments arranged by size:
3074 1907 552 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3860 3877 4030 4215 4660 325
Size: 1255 2280 17 153 185 445 1498
Fragments arranged by size:
2280 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4079 5791 96
Size: 274 80 33 51 1443 2102 1712 138
Fragments arranged by size:
2102 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2006 2038 5793 5825 2006
Size: 32 3755 32 2014
Fragments arranged by size:
3755 2014 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2716 2716
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI