Restriction map for
pFN21AE0123
(Circular) (Six-base) MAPSORT of: pFN21AE0123.seq Check: 1572 from: 1 to: 5572
pFN21AE0123 5572 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3382 278
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2915 1937
Size: 978 4594
Acc65I G'GTAC_C
Cuts at: 2893 2893
Size: 5572
AclI AA'CG_TT
Cuts at: 1563 3699 4072 1563
Size: 2136 373 3063
Fragments arranged by size:
3063 2136 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2885 3635 5019 5287 1632
Size: 1253 750 1384 268 1917
Fragments arranged by size:
1917 1384 1253 750 268
AfeI AGC'GCT
Cuts at: 2077 2077
Size: 5572
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5375
AflIII A'CryG_T
Cuts at: 1203 4471 5448 1203
Size: 3268 977 1327
Fragments arranged by size:
3268 1327 977
AgeI A'CCGG_T
Cuts at: 4403 4403
Size: 5572
AhdI GACnn_n'nnGTC
Cuts at: 4301 4301
Size: 5572
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5572
AlwNI CAG_nnn'CTG
Cuts at: 2788 3315 4887 2788
Size: 527 1572 3473
Fragments arranged by size:
3473 1572 527
ApaI G_GGCC'C
Cuts at: 2767 2797 2767
Size: 30 5542
ApaLI G'TGCA_C
Cuts at: 3629 4785 3629
Size: 1156 4416
ApoI r'AATT_y
Cuts at: 1493 2881 3107 3259 5368 1493
Size: 1388 226 152 2109 1697
Fragments arranged by size:
2109 1697 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4126 160
Size: 872 3094 1606
Fragments arranged by size:
3094 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 2444 2568 2897 1870
Size: 73 172 329 124 329 4545
Fragments arranged by size:
4545 329 329 172 124 73
BamHI G'GATC_C
Cuts at: 2479 2902 3269 2479
Size: 423 367 4782
Fragments arranged by size:
4782 423 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2810 2893 4348 618
Size: 325 217 627 1023 83 1455 1842
Fragments arranged by size:
1842 1455 1023 627 325 217 83
BanII G_rGCy'C
Cuts at: 1391 2445 2767 2797 2891 1391
Size: 1054 322 30 94 4072
Fragments arranged by size:
4072 1054 322 94 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4601
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3763 3797 1903
Size: 34 1826 34 3678
Fragments arranged by size:
3678 1826 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2293 3464 4674 2293
Size: 1171 1210 3191
Fragments arranged by size:
3191 1210 1171
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4183 136
Size: 107 122 71 631 605 2511 1525
Fragments arranged by size:
2511 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5567 5567
Size: 5572
BlpI GC'TnA_GC
Cuts at: 2989 2989
Size: 5572
Bme1580I G_kGCm'C
Cuts at: 1790 2767 2797 3633 4789 1790
Size: 977 30 836 1156 2573
Fragments arranged by size:
2573 1156 977 836 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5572
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3303 4261 442
Size: 416 53 83 399 24 1886 958 1753
Fragments arranged by size:
1886 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 2741 4232 1239
Size: 855 647 1491 2579
Fragments arranged by size:
2579 1491 855 647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2630 3697 4562 4860 2630
Size: 1067 865 298 3342
Fragments arranged by size:
3342 1067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4235 882
Size: 617 2736 2219
Fragments arranged by size:
2736 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3363 493
Size: 711 2159 2702
Fragments arranged by size:
2702 2159 711
BsaBI GATnn'nnATC
Cuts at: 5566 5566
Size: 5572
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2041 3379 3761 4396
Size: 53 83 186 936 508 1338 382 635
Cuts at: 4396 275
Size: 1451
Fragments arranged by size:
1451 1338 936 635 508 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4005 4403 4677 4824 5235 4005
Size: 398 274 147 411 4342
Fragments arranged by size:
4342 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2031 2061 2714 2744 1178
Size: 30 165 30 628 30 653 30 4006
Fragments arranged by size:
4006 653 628 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2453 2738 2741 1186
Size: 840 427 285 3 4017
Fragments arranged by size:
4017 840 427 285 3
BseYI C'CCAG_C
Cuts at: 2072 2249 2292 2549 2640 4775 5271 2072
Size: 177 43 257 91 2135 496 2373
Fragments arranged by size:
2373 2135 496 257 177 91 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4999
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2060 3022 3783 4811 1540
Size: 275 186 59 962 761 1028 2301
Fragments arranged by size:
2301 1028 962 761 275 186 59
BsiHKAI G_wGCw'C
Cuts at: 2580 2843 3633 3718 4789 2580
Size: 263 790 85 1071 3363
Fragments arranged by size:
3363 1071 790 263 85
BsmI GAATG_Cn'
Cuts at: 3083 3176 3083
Size: 93 5479
BsmBI CGTCTCn'nnnn_
Cuts at: 2051 2112 2051
Size: 61 5511
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2445 2580 2767 2797 2843 2891 3633
Size: 399 655 135 187 30 46 48 742
Cuts at: 3633 3718 4789 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 655 399 187 135 85
48 46 30
BspEI T'CCGG_A
Cuts at: 5235 5235
Size: 5572
BspHI T'CATG_A
Cuts at: 3461 5191 3461
Size: 1730 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2688 2927 844
Size: 1844 239 3489
Fragments arranged by size:
3489 1844 239
BsrBI CCG'CTC
Cuts at: 1394 1823 3459 1394
Size: 429 1636 3507
Fragments arranged by size:
3507 1636 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2265 4067 4241 65
Size: 1183 1017 1802 174 1396
Fragments arranged by size:
1802 1396 1183 1017 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 2807 4216 4403 1700
Size: 110 249 748 1409 187 2869
Fragments arranged by size:
2869 1409 748 249 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5572
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5572
BssSI C'ACGA_G
Cuts at: 3632 4644 3632
Size: 1012 4560
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2704 2788 794
Size: 1910 84 3578
Fragments arranged by size:
3578 1910 84
BstBI TT'CG_AA
Cuts at: 4376 4376
Size: 5572
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5572
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2542 2272
Size: 270 5302
BstYI r'GATC_y
Cuts at: 1972 2479 2736 2902 3269 3654 3671 5123 5209
Size: 507 257 166 367 385 17 1452 86
Cuts at: 5209 5221 5567 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 507 385 367 346 257 166
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2652 513
Size: 552 1587 3433
Fragments arranged by size:
3433 1587 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4833
Fragments arranged by size:
4833 683 56
BtsI GCAGTG_nn'
Cuts at: 2277 3088 3882 3902 2277
Size: 811 794 20 3947
Fragments arranged by size:
3947 811 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5537
DraI TTT'AAA
Cuts at: 2876 2876
Size: 5572
DraIII CAC_nnn'GTG
Cuts at: 2177 2337 2177
Size: 160 5412
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4579 809
Size: 507 3263 1802
Fragments arranged by size:
3263 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2057 2262 3019
Size: 54 1425 50 275 18 227 205 757
Cuts at: 3019 3908 5537 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 757 275 227 205 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2057 3019 1537
Size: 275 245 962 4090
Fragments arranged by size:
4090 962 275 245
EarI CTCTTCn'nnn_
Cuts at: 1353 2138 2822 3502 1353
Size: 785 684 680 3423
Fragments arranged by size:
3423 785 684 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4159 4533 4679 387
Size: 1064 138 2570 374 146 1280
Fragments arranged by size:
2570 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2741 2885 3635 4232 5019 5287
Size: 393 462 647 144 750 597 787 268
Cuts at: 5287 1239
Size: 1524
Fragments arranged by size:
1524 787 750 647 597 462 393 268
144
EcoNI CCTnn'n_nnAGG
Cuts at: 2723 2867 2723
Size: 144 5428
EcoO109I rG'GnC_Cy
Cuts at: 2257 2546 2257
Size: 289 5283
EcoRI G'AATT_C
Cuts at: 2881 3259 2881
Size: 378 5194
FspI TGC'GCA
Cuts at: 4078 4078
Size: 5572
HaeII r_GCGC'y
Cuts at: 1678 2079 4719 1678
Size: 401 2640 2531
Fragments arranged by size:
2640 2531 401
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2031 2063 2137 2169 2538
Size: 32 135 32 459 32 74 32 369
Cuts at: 2538 2570 4213 4245 4287 4319 1373
Size: 32 1643 32 42 32 2626
Fragments arranged by size:
2626 1643 459 369 135 74 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2916 3162 669
Size: 988 281 978 246 3079
Fragments arranged by size:
3079 988 978 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5572
HpaI GTT'AAC
Cuts at: 3162 3162
Size: 5572
KpnI G_GTAC'C
Cuts at: 2897 2897
Size: 5572
MfeI C'AATT_G
Cuts at: 3171 3171
Size: 5572
MluI A'CGCG_T
Cuts at: 5448 5448
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4686 4870 1305
Size: 3381 184 2007
Fragments arranged by size:
3381 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 10
Size: 54 1425 343 432 3318
Fragments arranged by size:
3318 1425 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2772 3530 3889 4048 518
Size: 648 1104 502 758 359 159 2042
Fragments arranged by size:
2042 1104 758 648 502 359 159
MspA1I CmG'CkG
Cuts at: 1606 2383 2988 3665 4813 5058 1606
Size: 777 605 677 1148 245 2120
Fragments arranged by size:
2120 1148 777 677 605 245
NaeI GCC'GGC
Cuts at: 2061 2809 2061
Size: 748 4824
NcoI C'CATG_G
Cuts at: 513 1065 2652 513
Size: 552 1587 3433
Fragments arranged by size:
3433 1587 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5572
NgoMIV G'CCGG_C
Cuts at: 2059 2807 2059
Size: 748 4824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5572
NotI GC'GGCC_GC
Cuts at: 3019 3019
Size: 5572
NspI r_CATG'y
Cuts at: 1132 1229 2930 4475 1132
Size: 97 1701 1545 2229
Fragments arranged by size:
2229 1701 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5572
PciI A'CATG_T
Cuts at: 4471 4471
Size: 5572
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5572
PmeI GTTT'AAAC
Cuts at: 2876 2876
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 5154 5186 3641
Size: 32 1481 32 4027
Fragments arranged by size:
4027 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2546 2546
Size: 5572
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5572
PsiI TTA'TAA
Cuts at: 3142 3142
Size: 5572
PspOMI G'GGCC_C
Cuts at: 2763 2793 2763
Size: 30 5542
PstI C_TGCA'G
Cuts at: 830 2784 2924 830
Size: 1954 140 3478
Fragments arranged by size:
3478 1954 140
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5572
PvuII CAG'CTG
Cuts at: 2383 2383
Size: 5572
SalI G'TCGA_C
Cuts at: 1936 2914 1936
Size: 978 4594
SanDI GG'GwC_CC
Cuts at: 2546 2546
Size: 5572
SbfI CC_TGCA'GG
Cuts at: 2924 2924
Size: 5572
ScaI AGT'ACT
Cuts at: 2312 3820 5532 2312
Size: 1508 1712 2352
Fragments arranged by size:
2352 1712 1508
SfcI C'TryA_G
Cuts at: 826 1045 2428 2780 2920 4055 4736 4927 826
Size: 219 1383 352 140 1135 681 191 1471
Fragments arranged by size:
1471 1383 1135 681 352 219 191 140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5572
SmaI CCC'GGG
Cuts at: 1872 2117 2899 1872
Size: 245 782 4545
Fragments arranged by size:
4545 782 245
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2645 3676 4577 4839 820
Size: 197 926 702 1031 901 262 1553
Fragments arranged by size:
1553 1031 926 901 702 262 197
SnaBI TAC'GTA
Cuts at: 493 3363 493
Size: 2870 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5572
SphI G_CATG'C
Cuts at: 1132 2930 1132
Size: 1798 3774
SrfI GCCC'GGGC
Cuts at: 2117 2117
Size: 5572
SspI AAT'ATT
Cuts at: 5 52 3496 5
Size: 47 3444 2081
Fragments arranged by size:
3444 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5572
StyI C'CwwG_G
Cuts at: 513 1065 2652 2664 3011 513
Size: 552 1587 12 347 3074
Fragments arranged by size:
3074 1587 552 347 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3599 3616 3769 3954 4399 325
Size: 1255 2019 17 153 185 445 1498
Fragments arranged by size:
2019 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 3818 5530
Size: 274 80 33 51 1443 333 1508 1712
Cuts at: 5530 96
Size: 138
Fragments arranged by size:
1712 1508 1443 333 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5532 5564 5532
Size: 32 5540
Tth111I GACn'n_nGTC
Cuts at: 1316 2043 1316
Size: 727 4845
XbaI T'CTAG_A
Cuts at: 2908 2908
Size: 5572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2490 2539 2257
Size: 233 49 5290
Fragments arranged by size:
5290 233 49
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5572
XmaI C'CCGG_G
Cuts at: 1870 2115 2897 1870
Size: 245 782 4545
Fragments arranged by size:
4545 782 245
XmnI GAAnn'nnTTC
Cuts at: 3701 3701
Size: 5572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3380 276
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SwaI