Restriction map for
pFN21AE0122
(Circular) (Six-base) MAPSORT of: pFN21AE0122.seq Check: 6231 from: 1 to: 5500
pFN21AE0122 5500 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3310 278
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2843 1937
Size: 906 4594
Acc65I G'GTAC_C
Cuts at: 2478 2821 2478
Size: 343 5157
AclI AA'CG_TT
Cuts at: 1563 3627 4000 1563
Size: 2064 373 3063
Fragments arranged by size:
3063 2064 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 3563 4947 5215 1632
Size: 638 1293 1384 268 1917
Fragments arranged by size:
1917 1384 1293 638 268
AfeI AGC'GCT
Cuts at: 2154 2154
Size: 5500
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5303
AflIII A'CryG_T
Cuts at: 1203 4399 5376 1203
Size: 3196 977 1327
Fragments arranged by size:
3196 1327 977
AgeI A'CCGG_T
Cuts at: 4331 4331
Size: 5500
AhdI GACnn_n'nnGTC
Cuts at: 4229 4229
Size: 5500
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5500
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2396 2428 2396
Size: 32 5468
AlwNI CAG_nnn'CTG
Cuts at: 2299 2356 2635 2737 3243 4815 2299
Size: 57 279 102 506 1572 2984
Fragments arranged by size:
2984 1572 506 279 102 57
ApaLI G'TGCA_C
Cuts at: 2489 3557 4713 2489
Size: 1068 1156 3276
Fragments arranged by size:
3276 1156 1068
ApoI r'AATT_y
Cuts at: 1493 2809 3035 3187 5296 1493
Size: 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4054 160
Size: 872 3022 1606
Fragments arranged by size:
3022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
AvaI C'yCGr_G
Cuts at: 1870 1943 2050 2424 2825 1870
Size: 73 107 374 401 4545
Fragments arranged by size:
4545 401 374 107 73
BamHI G'GATC_C
Cuts at: 2278 2830 3197 2278
Size: 552 367 4581
Fragments arranged by size:
4581 552 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2288 2478 2821 4276
Size: 325 217 627 315 186 190 343 1455
Cuts at: 4276 618
Size: 1842
Fragments arranged by size:
1842 1455 627 343 325 315 217 190
186
BanII G_rGCy'C
Cuts at: 1391 2049 2692 2746 2819 1391
Size: 658 643 54 73 4072
Fragments arranged by size:
4072 658 643 73 54
BbeI G_GCGC'C
Cuts at: 2106 2106
Size: 5500
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1999 2033 3691 3725 1903
Size: 34 62 34 1658 34 3678
Fragments arranged by size:
3678 1658 62 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3392 4602 3392
Size: 1210 4290
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4111 136
Size: 107 122 71 631 605 2439 1525
Fragments arranged by size:
2439 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5495 5495
Size: 5500
BlpI GC'TnA_GC
Cuts at: 2917 2917
Size: 5500
Bme1580I G_kGCm'C
Cuts at: 1790 2293 2493 3561 4717 1790
Size: 503 200 1068 1156 2573
Fragments arranged by size:
2573 1156 1068 503 200
BmgBI CAC'GTC
Cuts at: 1318 2785 1318
Size: 1467 4033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3231 4189 442
Size: 416 53 83 399 24 1814 958 1753
Fragments arranged by size:
1814 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2462 1056
Size: 1406 4094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 4160 1239
Size: 1094 1827 2579
Fragments arranged by size:
2579 1827 1094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 4490 4788 3625
Size: 865 298 4337
Fragments arranged by size:
4337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2222 2416 2653 4163 882
Size: 617 723 194 237 1510 2219
Fragments arranged by size:
2219 1510 723 617 237 194
BsaAI yAC'GTr
Cuts at: 493 1204 3291 493
Size: 711 2087 2702
Fragments arranged by size:
2702 2087 711
BsaBI GATnn'nnATC
Cuts at: 5494 5494
Size: 5500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 3307 3689 4324
Size: 53 83 186 936 570 1204 382 635
Cuts at: 4324 275
Size: 1451
Fragments arranged by size:
1451 1204 936 635 570 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3933 4331 4605 4752 5163 3933
Size: 398 274 147 411 4270
Fragments arranged by size:
4270 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 2447 2477 1178
Size: 30 165 30 993 30 21 30 4201
Fragments arranged by size:
4201 993 165 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2287 2468 2768 1186
Size: 1101 181 300 3918
Fragments arranged by size:
3918 1101 300 181
BseYI C'CCAG_C
Cuts at: 2512 2724 2758 4703 5199 2512
Size: 212 34 1945 496 2813
Fragments arranged by size:
2813 1945 496 212 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4927
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2377 2950 3711 4739 1540
Size: 275 186 376 573 761 1028 2301
Fragments arranged by size:
2301 1028 761 573 376 275 186
BsiHKAI G_wGCw'C
Cuts at: 2431 2493 3561 3646 4717 2431
Size: 62 1068 85 1071 3214
Fragments arranged by size:
3214 1071 1068 85 62
BsmI GAATG_Cn'
Cuts at: 2600 3011 3104 2600
Size: 411 93 4996
Fragments arranged by size:
4996 411 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2293 2431 2493 2692 2746 2819
Size: 399 259 244 138 62 199 54 73
Cuts at: 2819 3561 3646 4717 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 259 244 199 138
85 73 62 54
BspEI T'CCGG_A
Cuts at: 5163 5163
Size: 5500
BspHI T'CATG_A
Cuts at: 3389 5119 3389
Size: 1730 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2855 844
Size: 2011 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2719 3387 1394
Size: 429 896 668 3507
Fragments arranged by size:
3507 896 668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3995 4169 65
Size: 1183 2747 174 1396
Fragments arranged by size:
2747 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2105 2165 4144 4331 1700
Size: 110 295 60 1979 187 2869
Fragments arranged by size:
2869 1979 295 187 110 60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5500
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5500
BssSI C'ACGA_G
Cuts at: 3560 4572 3560
Size: 1012 4488
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2497 794
Size: 1703 3797
BstBI TT'CG_AA
Cuts at: 4304 4304
Size: 5500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5500
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2533
Size: 5500
BstYI r'GATC_y
Cuts at: 1972 2278 2295 2830 3197 3582 3599 5051 5137
Size: 306 17 535 367 385 17 1452 86
Cuts at: 5137 5149 5495 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 535 385 367 346 306 86
17 17 12
Bsu36I CC'TnA_GG
Cuts at: 2258 2738 2258
Size: 480 5020
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2663 513
Size: 552 939 168 491 3350
Fragments arranged by size:
3350 939 552 491 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2192 487
Size: 683 56 966 3795
Fragments arranged by size:
3795 966 683 56
BtsI GCAGTG_nn'
Cuts at: 3016 3810 3830 3016
Size: 794 20 4686
Fragments arranged by size:
4686 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5465
DraI TTT'AAA
Cuts at: 2804 2804
Size: 5500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4507 809
Size: 507 3191 1802
Fragments arranged by size:
3191 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2796 2947
Size: 54 1425 50 275 18 330 636 151
Cuts at: 2947 3836 5465 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 636 330 275 151 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2947 1537
Size: 275 1135 4090
Fragments arranged by size:
4090 1135 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3430 1353
Size: 2077 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4087 4461 4607 387
Size: 1064 138 2498 374 146 1280
Fragments arranged by size:
2498 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2333 3563 4160 4947 5215 1239
Size: 393 638 63 1230 597 787 268 1524
Fragments arranged by size:
1524 1230 787 638 597 393 268 63
EcoO109I rG'GnC_Cy
Cuts at: 2254 2254
Size: 5500
EcoRI G'AATT_C
Cuts at: 2809 3187 2809
Size: 378 5122
FspI TGC'GCA
Cuts at: 4006 4006
Size: 5500
HaeII r_GCGC'y
Cuts at: 1678 2106 2156 2186 2628 2725 4647 1678
Size: 428 50 30 442 97 1922 2531
Fragments arranged by size:
2531 1922 442 428 97 50 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2263 2295 4141 4173 4215
Size: 32 135 32 691 32 1846 32 42
Cuts at: 4215 4247 1373
Size: 32 2626
Fragments arranged by size:
2626 1846 691 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2844 3090 669
Size: 988 281 906 246 3079
Fragments arranged by size:
3079 988 906 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5500
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 5500
KasI G'GCGC_C
Cuts at: 2102 2102
Size: 5500
KpnI G_GTAC'C
Cuts at: 2482 2825 2482
Size: 343 5157
MfeI C'AATT_G
Cuts at: 3099 3099
Size: 5500
MluI A'CGCG_T
Cuts at: 5376 5376
Size: 5500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2641 4614 4798 1305
Size: 1336 1973 184 2007
Fragments arranged by size:
2007 1973 1336 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3678
Fragments arranged by size:
3678 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3458 3817 3976 518
Size: 648 2292 359 159 2042
Fragments arranged by size:
2292 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2143 2174 2476 2916 3593 4741 4986
Size: 456 81 31 302 440 677 1148 245
Cuts at: 4986 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 456 440 302 245 81
31
NaeI GCC'GGC
Cuts at: 2107 2167 2107
Size: 60 5440
NarI GG'CG_CC
Cuts at: 2103 2103
Size: 5500
NcoI C'CATG_G
Cuts at: 513 1065 2004 2663 513
Size: 552 939 659 3350
Fragments arranged by size:
3350 939 659 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5500
NgoMIV G'CCGG_C
Cuts at: 2105 2165 2105
Size: 60 5440
NheI G'CTAG_C
Cuts at: 1052 2458 1052
Size: 1406 4094
NotI GC'GGCC_GC
Cuts at: 2947 2947
Size: 5500
NruI TCG'CGA
Cuts at: 2379 2379
Size: 5500
NspI r_CATG'y
Cuts at: 1132 1229 2500 2858 4403 1132
Size: 97 1271 358 1545 2229
Fragments arranged by size:
2229 1545 1271 358 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5500
PciI A'CATG_T
Cuts at: 4399 4399
Size: 5500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 2794 1108
Size: 1101 585 3814
Fragments arranged by size:
3814 1101 585
PfoI T'CCnGG_A
Cuts at: 2394 2394
Size: 5500
PmeI GTTT'AAAC
Cuts at: 2804 2804
Size: 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2396 2428 3569 3601 5082 5114 2396
Size: 32 1141 32 1481 32 2782
Fragments arranged by size:
2782 1481 1141 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2254
Size: 5500
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5500
PsiI TTA'TAA
Cuts at: 3070 3070
Size: 5500
PstI C_TGCA'G
Cuts at: 830 2148 2304 2852 830
Size: 1318 156 548 3478
Fragments arranged by size:
3478 1318 548 156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5500
SacII CC_GC'GG
Cuts at: 2175 2175
Size: 5500
SalI G'TCGA_C
Cuts at: 1936 2842 1936
Size: 906 4594
SbfI CC_TGCA'GG
Cuts at: 2304 2852 2304
Size: 548 4952
ScaI AGT'ACT
Cuts at: 3748 5460 3748
Size: 1712 3788
SfcI C'TryA_G
Cuts at: 826 1045 2057 2144 2300 2848 3983 4664 4855
Size: 219 1012 87 156 548 1135 681 191
Cuts at: 4855 826
Size: 1471
Fragments arranged by size:
1471 1135 1012 681 548 219 191 156
87
SfoI GGC'GCC
Cuts at: 2104 2104
Size: 5500
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5500
SmaI CCC'GGG
Cuts at: 1872 2052 2827 1872
Size: 180 775 4545
Fragments arranged by size:
4545 775 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3604 4505 4767 820
Size: 197 926 1661 901 262 1553
Fragments arranged by size:
1661 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3291 493
Size: 2798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5500
SphI G_CATG'C
Cuts at: 1132 2858 1132
Size: 1726 3774
SspI AAT'ATT
Cuts at: 5 52 3424 5
Size: 47 3372 2081
Fragments arranged by size:
3372 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5500
StyI C'CwwG_G
Cuts at: 513 1065 2004 2663 2939 513
Size: 552 939 659 276 3074
Fragments arranged by size:
3074 939 659 552 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2363 2446 2585 2626 2791 3527 3544
Size: 1255 783 83 139 41 165 736 17
Cuts at: 3544 3697 3882 4327 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 783 736 445 185 165 153
139 83 41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2709 3746 5458
Size: 274 80 33 51 1443 732 1037 1712
Cuts at: 5458 96
Size: 138
Fragments arranged by size:
1712 1443 1037 732 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5460 5492 5460
Size: 32 5468
Tth111I GACn'n_nGTC
Cuts at: 1316 2210 1316
Size: 894 4606
XbaI T'CTAG_A
Cuts at: 2836 2836
Size: 5500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 2440 2530 2590 2335
Size: 105 90 60 5245
Fragments arranged by size:
5245 105 90 60
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5500
XmaI C'CCGG_G
Cuts at: 1870 2050 2825 1870
Size: 180 775 4545
Fragments arranged by size:
4545 775 180
XmnI GAAnn'nnTTC
Cuts at: 3629 3629
Size: 5500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3308 276
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI