Restriction map for
pFN21AE0120
(Circular) (Six-base) MAPSORT of: pFN21AE0120.seq Check: 2369 from: 1 to: 5566
pFN21AE0120 5566 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3376 278
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2580 2781 2909 1937
Size: 643 201 128 4594
Fragments arranged by size:
4594 643 201 128
Acc65I G'GTAC_C
Cuts at: 2887 2887
Size: 5566
AclI AA'CG_TT
Cuts at: 1563 3693 4066 1563
Size: 2130 373 3063
Fragments arranged by size:
3063 2130 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2291 3629 5013 5281 1632
Size: 659 1338 1384 268 1917
Fragments arranged by size:
1917 1384 1338 659 268
AflII C'TTAA_G
Cuts at: 820 1017 2816 820
Size: 197 1799 3570
Fragments arranged by size:
3570 1799 197
AflIII A'CryG_T
Cuts at: 1203 4465 5442 1203
Size: 3262 977 1327
Fragments arranged by size:
3262 1327 977
AgeI A'CCGG_T
Cuts at: 4397 4397
Size: 5566
AhdI GACnn_n'nnGTC
Cuts at: 4295 4295
Size: 5566
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5566
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2259 1184
Size: 1075 4491
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 2042
Size: 32 5534
AlwNI CAG_nnn'CTG
Cuts at: 2296 2719 2847 3309 4881 2296
Size: 423 128 462 1572 2981
Fragments arranged by size:
2981 1572 462 423 128
ApaI G_GGCC'C
Cuts at: 2169 2169
Size: 5566
ApaLI G'TGCA_C
Cuts at: 2084 2735 3623 4779 2084
Size: 651 888 1156 2871
Fragments arranged by size:
2871 1156 888 651
ApoI r'AATT_y
Cuts at: 1493 2875 3101 3253 5362 1493
Size: 1382 226 152 2109 1697
Fragments arranged by size:
2109 1697 1382 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4120 160
Size: 872 3088 1606
Fragments arranged by size:
3088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
AvaI C'yCGr_G
Cuts at: 1870 1943 2510 2891 1870
Size: 73 567 381 4545
Fragments arranged by size:
4545 567 381 73
AvrII C'CTAG_G
Cuts at: 2191 2191
Size: 5566
BamHI G'GATC_C
Cuts at: 2896 3263 2896
Size: 367 5199
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2887 4342 618
Size: 325 217 627 1100 1455 1842
Fragments arranged by size:
1842 1455 1100 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2153 2169 2473 2711 2885 1391
Size: 762 16 304 238 174 4072
Fragments arranged by size:
4072 762 304 238 174 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4595
BbvCI CC'TCA_GC
Cuts at: 2249 2424 2249
Size: 175 5391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3757 3791 1903
Size: 34 1820 34 3678
Fragments arranged by size:
3678 1820 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3458 4668 3458
Size: 1210 4356
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5566
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4177 136
Size: 107 122 71 631 605 2505 1525
Fragments arranged by size:
2505 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2826 5561 2826
Size: 2735 2831
BlpI GC'TnA_GC
Cuts at: 2983 2983
Size: 5566
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2169 2739 3627 4783 1790
Size: 298 81 570 888 1156 2573
Fragments arranged by size:
2573 1156 888 570 298 81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5566
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3297 4255 442
Size: 416 53 83 399 24 1880 958 1753
Fragments arranged by size:
1880 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5566
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 5566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2741 2824 2869 4226 1239
Size: 830 672 83 45 1357 2579
Fragments arranged by size:
2579 1357 830 672 83 45
Bpu10I CC'TnA_GC
Cuts at: 2249 2424 2249
Size: 175 5391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 2417 3691 4556 4854 2102
Size: 315 1274 865 298 2814
Fragments arranged by size:
2814 1274 865 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4229 882
Size: 617 2730 2219
Fragments arranged by size:
2730 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2734 3357 493
Size: 711 1530 623 2702
Fragments arranged by size:
2702 1530 711 623
BsaBI GATnn'nnATC
Cuts at: 5560 5560
Size: 5566
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2772 3373 3755 4390
Size: 53 83 186 936 1239 601 382 635
Cuts at: 4390 275
Size: 1451
Fragments arranged by size:
1451 1239 936 635 601 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3999 4397 4671 4818 5229 3999
Size: 398 274 147 411 4336
Fragments arranged by size:
4336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 2813 2843 1178
Size: 30 165 30 639 30 741 30 3901
Fragments arranged by size:
3901 741 639 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2069 1186
Size: 880 3 4683
Fragments arranged by size:
4683 880 3
BseYI C'CCAG_C
Cuts at: 2659 2663 4769 5265 2659
Size: 4 2106 496 2960
Fragments arranged by size:
2960 2106 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2057 2282 1163
Size: 573 321 225 4447
Fragments arranged by size:
4447 573 321 225
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3016 3777 4805 1540
Size: 275 186 1015 761 1028 2301
Fragments arranged by size:
2301 1028 1015 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 2739 3627 3712 4783 2088
Size: 651 888 85 1071 2871
Fragments arranged by size:
2871 1071 888 651 85
BsmI GAATG_Cn'
Cuts at: 3077 3170 3077
Size: 93 5473
BsmBI CGTCTCn'nnnn_
Cuts at: 2797 2797
Size: 5566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2153 2169 2473 2711 2739 2885
Size: 399 298 65 16 304 238 28 146
Cuts at: 2885 3627 3712 4783 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 304 298 238 146
85 65 28 16
BspEI T'CCGG_A
Cuts at: 5229 5229
Size: 5566
BspHI T'CATG_A
Cuts at: 3455 5185 3455
Size: 1730 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 2574 2921 844
Size: 1544 186 347 3489
Fragments arranged by size:
3489 1544 347 186
BsrBI CCG'CTC
Cuts at: 1394 1823 2038 3453 1394
Size: 429 215 1415 3507
Fragments arranged by size:
3507 1415 429 215
BsrDI GCAATG_nn'
Cuts at: 65 1248 4061 4235 65
Size: 1183 2813 174 1396
Fragments arranged by size:
2813 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2478 4210 4397 1700
Size: 110 668 1732 187 2869
Fragments arranged by size:
2869 1732 668 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5566
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5566
BssSI C'ACGA_G
Cuts at: 3626 4638 3626
Size: 1012 4554
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2629 794
Size: 1835 3731
BstBI TT'CG_AA
Cuts at: 4370 4370
Size: 5566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5566
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2017
Size: 5566
BstYI r'GATC_y
Cuts at: 1972 2340 2826 2896 3263 3648 3665 5117 5203
Size: 368 486 70 367 385 17 1452 86
Cuts at: 5203 5215 5561 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 486 385 368 367 346 86
70 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4075
Fragments arranged by size:
4075 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4827
Fragments arranged by size:
4827 683 56
BtsI GCAGTG_nn'
Cuts at: 2330 2454 3082 3876 3896 2330
Size: 124 628 794 20 4000
Fragments arranged by size:
4000 794 628 124 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5531
DraI TTT'AAA
Cuts at: 2870 2870
Size: 5566
DraIII CAC_nnn'GTG
Cuts at: 2092 2743 2092
Size: 651 4915
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2570 2777 4573 809
Size: 507 1254 207 1796 1802
Fragments arranged by size:
1802 1796 1254 507 207
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3013 3902
Size: 54 1425 50 275 18 177 1006 889
Cuts at: 3902 5531 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1006 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3013 1537
Size: 275 1201 4090
Fragments arranged by size:
4090 1201 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3496 1353
Size: 2143 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2048 2273 2645 4153 4527 4673
Size: 1064 138 459 225 372 1508 374 146
Cuts at: 4673 387
Size: 1280
Fragments arranged by size:
1508 1280 1064 459 374 372 225 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2291 2741 2824 2869 3629 4226
Size: 393 437 222 450 83 45 760 597
Cuts at: 4226 5013 5281 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 760 597 450 437 393 268
222 83 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2252 2120
Size: 132 5434
EcoO109I rG'GnC_Cy
Cuts at: 2165 2428 2165
Size: 263 5303
EcoRI G'AATT_C
Cuts at: 2875 3253 2875
Size: 378 5188
FspI TGC'GCA
Cuts at: 4072 4072
Size: 5566
HaeII r_GCGC'y
Cuts at: 1678 2424 2719 4713 1678
Size: 746 295 1994 2531
Fragments arranged by size:
2531 1994 746 295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2813 2845 4207 4239 4281
Size: 32 135 32 1241 32 1362 32 42
Cuts at: 4281 4313 1373
Size: 32 2626
Fragments arranged by size:
2626 1362 1241 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2575 2910 3156 669
Size: 988 281 637 335 246 3079
Fragments arranged by size:
3079 988 637 335 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5566
HpaI GTT'AAC
Cuts at: 3156 3156
Size: 5566
KpnI G_GTAC'C
Cuts at: 2891 2891
Size: 5566
MfeI C'AATT_G
Cuts at: 3165 3165
Size: 5566
MluI A'CGCG_T
Cuts at: 5442 5442
Size: 5566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2586 4680 4864 1305
Size: 1281 2094 184 2007
Fragments arranged by size:
2094 2007 1281 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3567
Fragments arranged by size:
3567 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 2537 3524 3883 4042 518
Size: 648 849 522 987 359 159 2042
Fragments arranged by size:
2042 987 849 648 522 359 159
MspA1I CmG'CkG
Cuts at: 1606 2064 2104 2199 2293 2461 2526 2620 2663
Size: 458 40 95 94 168 65 94 43
Cuts at: 2663 2982 3659 4807 5052 1606
Size: 319 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 458 319 245 168 95
94 94 65 43 40
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4075
Fragments arranged by size:
4075 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5566
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5566
NotI GC'GGCC_GC
Cuts at: 3013 3013
Size: 5566
NspI r_CATG'y
Cuts at: 1132 1229 2627 2924 4469 1132
Size: 97 1398 297 1545 2229
Fragments arranged by size:
2229 1545 1398 297 97
PasI CC'CwG_GG
Cuts at: 1348 2687 1348
Size: 1339 4227
PciI A'CATG_T
Cuts at: 4465 4465
Size: 5566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2847 1108
Size: 1739 3827
PfoI T'CCnGG_A
Cuts at: 2310 2343 2310
Size: 33 5533
PmeI GTTT'AAAC
Cuts at: 2870 2870
Size: 5566
PmlI CAC'GTG
Cuts at: 2734 2734
Size: 5566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2042 2074 3635 3667 5148 5180 2042
Size: 32 1561 32 1481 32 2428
Fragments arranged by size:
2428 1561 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2428 2428
Size: 5566
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5566
PsiI TTA'TAA
Cuts at: 3136 3136
Size: 5566
PspOMI G'GGCC_C
Cuts at: 2165 2165
Size: 5566
PstI C_TGCA'G
Cuts at: 830 2109 2238 2337 2460 2634 2802 2918 830
Size: 1279 129 99 123 174 168 116 3478
Fragments arranged by size:
3478 1279 174 168 129 123 116 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5566
PvuII CAG'CTG
Cuts at: 2104 2199 2293 2461 2620 2663 2104
Size: 95 94 168 159 43 5007
Fragments arranged by size:
5007 168 159 95 94 43
SalI G'TCGA_C
Cuts at: 1936 2908 1936
Size: 972 4594
SbfI CC_TGCA'GG
Cuts at: 2918 2918
Size: 5566
ScaI AGT'ACT
Cuts at: 3814 5526 3814
Size: 1712 3854
SexAI A'CCwGG_T
Cuts at: 2211 2211
Size: 5566
SfcI C'TryA_G
Cuts at: 826 1045 2105 2234 2333 2456 2630 2798 2854
Size: 219 1060 129 99 123 174 168 56
Cuts at: 2854 2914 4049 4730 4921 826
Size: 60 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 681 219 191 174 168
129 123 99 60 56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5566
SmaI CCC'GGG
Cuts at: 1872 2893 1872
Size: 1021 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 2432 2816 3670 4571 4833
Size: 197 926 174 315 384 854 901 262
Cuts at: 4833 820
Size: 1553
Fragments arranged by size:
1553 926 901 854 384 315 262 197
174
SnaBI TAC'GTA
Cuts at: 493 3357 493
Size: 2864 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5566
SphI G_CATG'C
Cuts at: 1132 2627 2924 1132
Size: 1495 297 3774
Fragments arranged by size:
3774 1495 297
SspI AAT'ATT
Cuts at: 5 52 3490 5
Size: 47 3438 2081
Fragments arranged by size:
3438 2081 47
StuI AGG'CCT
Cuts at: 1517 2126 1517
Size: 609 4957
StyI C'CwwG_G
Cuts at: 513 1065 2004 2191 3005 513
Size: 552 939 187 814 3074
Fragments arranged by size:
3074 939 814 552 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3593 3610 3763 3948 4393 325
Size: 1255 2013 17 153 185 445 1498
Fragments arranged by size:
2013 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3812 5524 96
Size: 274 80 33 51 1443 1835 1712 138
Fragments arranged by size:
1835 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2309 2341 5526 5558 2309
Size: 32 3185 32 2317
Fragments arranged by size:
3185 2317 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5566
XbaI T'CTAG_A
Cuts at: 2902 2902
Size: 5566
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5566
XmaI C'CCGG_G
Cuts at: 1870 2891 1870
Size: 1021 4545
XmnI GAAnn'nnTTC
Cuts at: 3695 3695
Size: 5566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3374 276
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI