Restriction map for
pFN21AE0116
(Circular) (Six-base) MAPSORT of: pFN21AE0116.seq Check: 8794 from: 1 to: 5875
pFN21AE0116 5875 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3218 1937
Size: 1281 4594
Acc65I G'GTAC_C
Cuts at: 2613 3196 2613
Size: 583 5292
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2181 2321 2537 2633 3058 3938 5322 5590
Size: 549 140 216 96 425 880 1384 268
Cuts at: 5590 1632
Size: 1917
Fragments arranged by size:
1917 1384 880 549 425 268 216 140
96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5875
AlwNI CAG_nnn'CTG
Cuts at: 2337 3618 5190 2337
Size: 1281 1572 3022
Fragments arranged by size:
3022 1572 1281
ApaLI G'TGCA_C
Cuts at: 2042 3932 5088 2042
Size: 1890 1156 2829
Fragments arranged by size:
2829 1890 1156
ApoI r'AATT_y
Cuts at: 1493 2935 3013 3184 3410 3562 5671 1493
Size: 1442 78 171 226 152 2109 1697
Fragments arranged by size:
2109 1697 1442 226 171 152 78
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 3087 3106 3200 1870
Size: 73 1144 19 94 4545
Fragments arranged by size:
4545 1144 94 73 19
AvrII C'CTAG_G
Cuts at: 2386 2386
Size: 5875
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2011 2044 2011
Size: 33 5842
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2613 3196 4651 618
Size: 325 217 627 826 583 1455 1842
Fragments arranged by size:
1842 1455 826 627 583 325 217
BanII G_rGCy'C
Cuts at: 1391 2982 3194 1391
Size: 1591 212 4072
Fragments arranged by size:
4072 1591 212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2156 2531 928
Size: 971 257 375 4272
Fragments arranged by size:
4272 971 375 257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3130 3164 4066 4100 1903
Size: 34 1193 34 902 34 3678
Fragments arranged by size:
3678 1193 902 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BfrBI ATG'CAT
Cuts at: 3025 3025
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2138 2744 4486
Size: 107 122 71 631 605 466 606 1742
Cuts at: 4486 136
Size: 1525
Fragments arranged by size:
1742 1525 631 606 605 466 122 107
71
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2046 3936 5092 1790
Size: 256 1890 1156 2573
Fragments arranged by size:
2573 1890 1156 256
BmgBI CAC'GTC
Cuts at: 1318 2041 1318
Size: 723 5152
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3606 4564 442
Size: 416 53 83 399 24 2189 958 1753
Fragments arranged by size:
2189 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2414 2414
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2283 2915 3023 4535 1239
Size: 839 205 632 108 1512 2579
Fragments arranged by size:
2579 1512 839 632 205 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2539 2873 2966 4000 4865 5163 2539
Size: 334 93 1034 865 298 3251
Fragments arranged by size:
3251 1034 865 334 298 93
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4538 882
Size: 617 3039 2219
Fragments arranged by size:
3039 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3666 493
Size: 711 2462 2702
Fragments arranged by size:
2702 2462 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2964 3682 4064 4699
Size: 53 83 186 936 1431 718 382 635
Cuts at: 4699 275
Size: 1451
Fragments arranged by size:
1451 1431 936 718 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3030 4308 4706 4980 5127 5538 3030
Size: 1278 398 274 147 411 3367
Fragments arranged by size:
3367 1278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2334 2364 1178
Size: 30 165 30 931 30 4689
Fragments arranged by size:
4689 931 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 2672 1186
Size: 1057 429 4389
Fragments arranged by size:
4389 1057 429
BseYI C'CCAG_C
Cuts at: 5078 5574 5078
Size: 496 5379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 1163
Size: 573 816 4486
Fragments arranged by size:
4486 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4086 5114 1540
Size: 275 186 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2982 3936 4021 5092 2046
Size: 936 954 85 1071 2829
Fragments arranged by size:
2829 1071 954 936 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
BsmBI CGTCTCn'nnnn_
Cuts at: 2717 2717
Size: 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2982 3194 3936 4021 5092 1391
Size: 399 256 936 212 742 85 1071 2174
Fragments arranged by size:
2174 1071 936 742 399 256 212 85
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 3764 5494 3764
Size: 1730 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3230 844
Size: 2386 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 1394
Size: 429 1939 3507
Fragments arranged by size:
3507 1939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2610 4519 4706 1700
Size: 110 800 1909 187 2869
Fragments arranged by size:
2869 1909 800 187 110
BsrGI T'GTAC_A
Cuts at: 96 2466 96
Size: 2370 3505
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 2279 3935 4947 2279
Size: 1656 1012 3207
Fragments arranged by size:
3207 1656 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2539 794
Size: 1745 4130
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5875
BstXI CCAn_nnnn'nTGG
Cuts at: 2180 2180
Size: 5875
BstYI r'GATC_y
Cuts at: 1972 2865 3205 3572 3957 3974 5426 5512 5524
Size: 893 340 367 385 17 1452 86 12
Cuts at: 5524 5870 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 893 385 367 346 340 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2481 2481
Size: 5875
BtgI C'CryG_G
Cuts at: 513 1065 2160 513
Size: 552 1095 4228
Fragments arranged by size:
4228 1095 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 3391 4185 4205 3391
Size: 794 20 5061
Fragments arranged by size:
5061 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2544 2847 3322
Size: 54 1425 50 275 18 714 303 475
Cuts at: 3322 4211 5840 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 714 475 303 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3322 1537
Size: 275 1510 4090
Fragments arranged by size:
4090 1510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2297 3805 1353
Size: 944 1508 3423
Fragments arranged by size:
3423 1508 944
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2980 2980
Size: 5875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2181 2283 2321 2537 2633 2915
Size: 393 446 103 102 38 216 96 282
Cuts at: 2915 3023 3058 3938 4535 5322 5590 1239
Size: 108 35 880 597 787 268 1524
Fragments arranged by size:
1524 880 787 597 446 393 282 268
216 108 103 102 96 38 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2837 2837
Size: 5875
EcoRI G'AATT_C
Cuts at: 2935 3184 3562 2935
Size: 249 378 5248
Fragments arranged by size:
5248 378 249
EcoRV GAT'ATC
Cuts at: 3123 3123
Size: 5875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 5875
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 2710 5022 1678
Size: 1032 2312 2531
Fragments arranged by size:
2531 2312 1032
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2716 2748 4516 4548 4590
Size: 32 135 32 1144 32 1768 32 42
Cuts at: 4590 4622 1373
Size: 32 2626
Fragments arranged by size:
2626 1768 1144 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2372 2549 748
Size: 1624 177 4074
Fragments arranged by size:
4074 1624 177
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 2617 3200 2617
Size: 583 5292
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2671 4989 5173 1305
Size: 1366 2318 184 2007
Fragments arranged by size:
2318 2007 1366 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2546 10
Size: 54 1425 343 714 3339
Fragments arranged by size:
3339 1425 714 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 2877 3161 3833 4192 4351 518
Size: 648 1074 637 284 672 359 159 2042
Fragments arranged by size:
2042 1074 672 648 637 359 284 159
MspA1I CmG'CkG
Cuts at: 1606 2180 2299 2902 3291 3968 5116 5361 1606
Size: 574 119 603 389 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 603 574 389 245 119
NcoI C'CATG_G
Cuts at: 513 1065 2160 513
Size: 552 1095 4228
Fragments arranged by size:
4228 1095 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NsiI A_TGCA'T
Cuts at: 3027 3027
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 2887 3233 4778 1132
Size: 97 1658 346 1545 2229
Fragments arranged by size:
2229 1658 1545 346 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2875 1108
Size: 1767 4108
PfoI T'CCnGG_A
Cuts at: 2322 2352 2973 2322
Size: 30 621 5224
Fragments arranged by size:
5224 621 30
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PstI C_TGCA'G
Cuts at: 830 2703 3227 830
Size: 1873 524 3478
Fragments arranged by size:
3478 1873 524
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 2180 2299 2180
Size: 119 5756
SacI G_AGCT'C
Cuts at: 2982 2982
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SfcI C'TryA_G
Cuts at: 826 1045 2342 2699 3223 4358 5039 5230 826
Size: 219 1297 357 524 1135 681 191 1471
Fragments arranged by size:
1471 1297 1135 681 524 357 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3089 3108 3202 1872
Size: 1217 19 94 4545
Fragments arranged by size:
4545 1217 94 19
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2518 2888 2981 3979 4880 5142
Size: 197 926 575 370 93 998 901 262
Cuts at: 5142 820
Size: 1553
Fragments arranged by size:
1553 998 926 901 575 370 262 197
93
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SrfI GCCC'GGGC
Cuts at: 3089 3089
Size: 5875
SspI AAT'ATT
Cuts at: 5 52 2290 3799 5
Size: 47 2238 1509 2081
Fragments arranged by size:
2238 2081 1509 47
StuI AGG'CCT
Cuts at: 1517 2738 1517
Size: 1221 4654
StyI C'CwwG_G
Cuts at: 513 1065 2160 2386 3314 513
Size: 552 1095 226 928 3074
Fragments arranged by size:
3074 1095 928 552 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2061 3095 3902 3919 4072 4257 4702
Size: 1255 481 1034 807 17 153 185 445
Cuts at: 4702 325
Size: 1498
Fragments arranged by size:
1498 1255 1034 807 481 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 2316 2466
Size: 274 80 33 51 1443 267 72 150
Cuts at: 2466 4121 5833 96
Size: 1655 1712 138
Fragments arranged by size:
1712 1655 1443 274 267 150 138 80
72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5835 5867 5835
Size: 32 5843
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 3087 3106 3200 1870
Size: 1217 19 94 4545
Fragments arranged by size:
4545 1217 94 19
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI Bpu10I
BsiWI BstZ17I DraIII EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SwaI XcmI