Restriction map for
pFN21AE0111
(Circular) (Six-base) MAPSORT of: pFN21AE0111.seq Check: 8119 from: 1 to: 5743
pFN21AE0111 5743 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2138 2332 3806 5190 5458 1632
Size: 506 194 1474 1384 268 1917
Fragments arranged by size:
1917 1474 1384 506 268 194
AfeI AGC'GCT
Cuts at: 2345 2345
Size: 5743
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 2058 4472 2058
Size: 2414 3329
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2733 1184
Size: 1549 4194
AlwNI CAG_nnn'CTG
Cuts at: 2486 2671 2857 2899 3486 5058 2486
Size: 185 186 42 587 1572 3171
Fragments arranged by size:
3171 1572 587 186 185 42
ApaI G_GGCC'C
Cuts at: 2018 2037 2149 2018
Size: 19 112 5612
Fragments arranged by size:
5612 112 19
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2102 2246 2436 3068 1870
Size: 73 86 73 144 190 632 4545
Fragments arranged by size:
4545 632 190 144 86 73 73
AvrII C'CTAG_G
Cuts at: 2260 2260
Size: 5743
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2097 2189 3064 4519 618
Size: 325 217 627 310 92 875 1455 1842
Fragments arranged by size:
1842 1455 875 627 325 310 217 92
BanII G_rGCy'C
Cuts at: 1391 2018 2037 2149 2641 2947 3062 1391
Size: 627 19 112 492 306 115 4072
Fragments arranged by size:
4072 627 492 306 115 112 19
BbeI G_GCGC'C
Cuts at: 2101 2101
Size: 5743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3016 928
Size: 971 1117 3655
Fragments arranged by size:
3655 1117 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 1903
Size: 34 1997 34 3678
Fragments arranged by size:
3678 1997 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2220 4354 136
Size: 107 122 71 631 605 548 2134 1525
Fragments arranged by size:
2134 1525 631 605 548 122 107 71
BglII A'GATC_T
Cuts at: 3003 5738 3003
Size: 2735 3008
BlpI GC'TnA_GC
Cuts at: 2961 3160 2961
Size: 199 5544
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2037 2149 3804 4960 1790
Size: 228 19 112 1655 1156 2573
Fragments arranged by size:
2573 1655 1156 228 112 19
BmgBI CAC'GTC
Cuts at: 1318 2233 1318
Size: 915 4828
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2438 2864 4403 1239
Size: 986 213 426 1539 2579
Fragments arranged by size:
2579 1539 986 426 213
Bpu10I CC'TnA_GC
Cuts at: 2580 2580
Size: 5743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2076 2264 3868 4733 5031 2076
Size: 188 1604 865 298 2788
Fragments arranged by size:
2788 1604 865 298 188
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2098 2223 3550 3932
Size: 53 83 186 936 565 125 1327 382
Cuts at: 3932 4567 275
Size: 635 1451
Fragments arranged by size:
1451 1327 936 635 565 382 186 125
83 53
BsaWI w'CCGG_w
Cuts at: 2970 4176 4574 4848 4995 5406 2970
Size: 1206 398 274 147 411 3307
Fragments arranged by size:
3307 1206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2146 2176 1178
Size: 30 165 30 743 30 4745
Fragments arranged by size:
4745 743 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3005 1186
Size: 1819 3924
BseYI C'CCAG_C
Cuts at: 2448 2553 2924 4946 5442 2448
Size: 105 371 2022 496 2749
Fragments arranged by size:
2749 2022 496 371 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3002 1163
Size: 573 1266 3904
Fragments arranged by size:
3904 1266 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2081 2143 3193 3954 4982 1540
Size: 275 186 80 62 1050 761 1028 2301
Fragments arranged by size:
2301 1050 1028 761 275 186 80 62
BsiHKAI G_wGCw'C
Cuts at: 2712 3804 3889 4960 2712
Size: 1092 85 1071 3495
Fragments arranged by size:
3495 1092 1071 85
BsmI GAATG_Cn'
Cuts at: 2650 3254 3347 2650
Size: 604 93 5046
Fragments arranged by size:
5046 604 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2037 2149 2641 2712 2947 3062
Size: 399 228 19 112 492 71 235 115
Cuts at: 3062 3804 3889 4960 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 399 235 228 115
112 85 71 19
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2656 2974 3098 844
Size: 1812 318 124 3489
Fragments arranged by size:
3489 1812 318 124
BsrBI CCG'CTC
Cuts at: 1394 1823 2162 3630 1394
Size: 429 339 1468 3507
Fragments arranged by size:
3507 1468 429 339
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 2622 3803 4815 2622
Size: 1181 1012 3550
Fragments arranged by size:
3550 1181 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2627 794
Size: 1833 3910
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 2462 2462
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2199 2253 3003 3073 3440 3825 3842 5294
Size: 227 54 750 70 367 385 17 1452
Cuts at: 5294 5380 5392 5738 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 750 385 367 346 227 86
70 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2295 2318 2295
Size: 23 5720
BtgI C'CryG_G
Cuts at: 513 1065 2167 513
Size: 552 1102 4089
Fragments arranged by size:
4089 1102 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 2660 3259 4053 4073 2660
Size: 599 794 20 4330
Fragments arranged by size:
4330 794 599 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2730 2765 529
Size: 35 2166 35 3507
Fragments arranged by size:
3507 2166 35 35
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2078 2212 3190
Size: 54 1425 50 275 18 248 134 978
Cuts at: 3190 4079 5708 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 978 889 275 248 134 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2078 3190 1537
Size: 275 266 1112 4090
Fragments arranged by size:
4090 1112 275 266
EarI CTCTTCn'nnn_
Cuts at: 1353 3673 1353
Size: 2320 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2338 4330 4704 4850 387
Size: 1064 138 749 1992 374 146 1280
Fragments arranged by size:
1992 1280 1064 749 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2225 2332 2438 2864 3806 4403
Size: 393 506 87 107 106 426 942 597
Cuts at: 4403 5190 5458 1239
Size: 787 268 1524
Fragments arranged by size:
1524 942 787 597 506 426 393 268
107 106 87
EcoO109I rG'GnC_Cy
Cuts at: 2014 2033 2146 2264 2271 2290 2388 2570 2668
Size: 19 113 118 7 19 98 182 98
Cuts at: 2668 2764 2014
Size: 96 4993
Fragments arranged by size:
4993 182 118 113 98 98 96 19
19 7
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 5743
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2101 2347 2410 4890 1678
Size: 423 246 63 2480 2531
Fragments arranged by size:
2531 2480 423 246 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2044 2074 2076
Size: 32 135 32 431 32 9 30 2
Cuts at: 2076 2106 2995 3027 4384 4416 4458 4490 1373
Size: 30 889 32 1357 32 42 32 2626
Fragments arranged by size:
2626 1357 889 431 135 42 32 32
32 32 32 32 30 30 9 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KasI G'GCGC_C
Cuts at: 2097 2097
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 2733 4857 5041 1305
Size: 858 570 2124 184 2007
Fragments arranged by size:
2124 2007 858 570 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2715 2837 3701 4060 4219 518
Size: 648 1549 122 864 359 159 2042
Fragments arranged by size:
2042 1549 864 648 359 159 122
MspA1I CmG'CkG
Cuts at: 1606 2514 2695 2854 2896 3159 3836 4984 5229
Size: 908 181 159 42 263 677 1148 245
Cuts at: 5229 1606
Size: 2120
Fragments arranged by size:
2120 1148 908 677 263 245 181 159
42
NarI GG'CG_CC
Cuts at: 2098 2098
Size: 5743
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5191
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 2842 3101 4646 1132
Size: 97 1613 259 1545 2229
Fragments arranged by size:
2229 1613 1545 259 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2857 1108
Size: 1749 3994
PfoI T'CCnGG_A
Cuts at: 2202 2454 2202
Size: 252 5491
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2264 2290 2570 2668 2764 2264
Size: 26 280 98 96 5243
Fragments arranged by size:
5243 280 98 96 26
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2014 2033 2145 2014
Size: 19 112 5612
Fragments arranged by size:
5612 112 19
PspXI vC'TCGA_Gb
Cuts at: 2102 2246 2102
Size: 144 5599
PstI C_TGCA'G
Cuts at: 830 2667 3095 830
Size: 1837 428 3478
Fragments arranged by size:
3478 1837 428
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
PvuII CAG'CTG
Cuts at: 2896 2896
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SexAI A'CCwGG_T
Cuts at: 2566 2566
Size: 5743
SfcI C'TryA_G
Cuts at: 826 1045 2071 2663 3091 4226 4907 5098 826
Size: 219 1026 592 428 1135 681 191 1471
Fragments arranged by size:
1471 1135 1026 681 592 428 219 191
SfoI GGC'GCC
Cuts at: 2099 2099
Size: 5743
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 2438 3070 1872
Size: 566 632 4545
Fragments arranged by size:
4545 632 566
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2091 2102 2246 2279 3847 4748
Size: 197 926 148 11 144 33 1568 901
Cuts at: 4748 5010 820
Size: 262 1553
Fragments arranged by size:
1568 1553 926 901 262 197 148 144
33 11
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 2842 3101 1132
Size: 1710 259 3774
Fragments arranged by size:
3774 1710 259
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 2592 1517
Size: 1075 4668
StyI C'CwwG_G
Cuts at: 513 1065 2260 3182 513
Size: 552 1195 922 3074
Fragments arranged by size:
3074 1195 922 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 2428 3770 3787 3940 4125 4570
Size: 1255 591 257 1342 17 153 185 445
Cuts at: 4570 325
Size: 1498
Fragments arranged by size:
1498 1342 1255 591 445 257 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3989 5701 96
Size: 274 80 33 51 1443 2012 1712 138
Fragments arranged by size:
2012 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2146 2178 2652 2684 5703 5735 2146
Size: 32 474 32 3019 32 2154
Fragments arranged by size:
3019 2154 474 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2482 1316
Size: 1166 4577
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 2102 2246 1943
Size: 159 144 5440
Fragments arranged by size:
5440 159 144
XmaI C'CCGG_G
Cuts at: 1870 2436 3068 1870
Size: 566 632 4545
Fragments arranged by size:
4545 632 566
XmnI GAAnn'nnTTC
Cuts at: 2345 3872 2345
Size: 1527 4216
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI
SacII SanDI SapI SfiI SrfI SwaI XcmI