(Circular) (Six-base) MAPSORT of: pFN21AE0110.seq Check: 2787 from: 1 to: 5482 pFN21AE0110 5482 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3292 278 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2825 1937 Size: 888 4594 Acc65I G'GTAC_C Cuts at: 2803 2803 Size: 5482 AclI AA'CG_TT Cuts at: 1563 3609 3982 1563 Size: 2046 373 3063 Fragments arranged by size: 3063 2046 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 3545 4929 5197 1632 Size: 597 1316 1384 268 1917 Fragments arranged by size: 1917 1384 1316 597 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5285 AflIII A'CryG_T Cuts at: 1203 4381 5358 1203 Size: 3178 977 1327 Fragments arranged by size: 3178 1327 977 AgeI A'CCGG_T Cuts at: 4313 4313 Size: 5482 AhdI GACnn_n'nnGTC Cuts at: 4211 4211 Size: 5482 AleI CACnn'nnGTG Cuts at: 1166 2531 1166 Size: 1365 4117 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5482 AlwNI CAG_nnn'CTG Cuts at: 3225 4797 3225 Size: 1572 3910 ApaLI G'TGCA_C Cuts at: 3539 4695 3539 Size: 1156 4326 ApoI r'AATT_y Cuts at: 1493 2195 2379 2791 3017 3169 5278 1493 Size: 702 184 412 226 152 2109 1697 Fragments arranged by size: 2109 1697 702 412 226 184 152 AseI AT'TA_AT Cuts at: 160 1032 2712 4036 160 Size: 872 1680 1324 1606 Fragments arranged by size: 1680 1606 1324 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 AvaI C'yCGr_G Cuts at: 1870 1943 2807 1870 Size: 73 864 4545 Fragments arranged by size: 4545 864 73 BamHI G'GATC_C Cuts at: 2812 3179 2812 Size: 367 5115 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2356 2803 4258 618 Size: 325 217 627 569 447 1455 1842 Fragments arranged by size: 1842 1455 627 569 447 325 217 BanII G_rGCy'C Cuts at: 1391 2644 2801 1391 Size: 1253 157 4072 Fragments arranged by size: 4072 1253 157 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2555 928 Size: 971 656 3855 Fragments arranged by size: 3855 971 656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3673 3707 1903 Size: 34 1736 34 3678 Fragments arranged by size: 3678 1736 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3374 4584 3374 Size: 1210 4272 BclI T'GATC_A Cuts at: 1548 1548 Size: 5482 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4093 136 Size: 107 122 71 631 605 2421 1525 Fragments arranged by size: 2421 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2041 5477 2041 Size: 3436 2046 BlpI GC'TnA_GC Cuts at: 2467 2899 2467 Size: 432 5050 Bme1580I G_kGCm'C Cuts at: 1790 2299 2579 3543 4699 1790 Size: 509 280 964 1156 2573 Fragments arranged by size: 2573 1156 964 509 280 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5482 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2688 3213 4171 Size: 416 53 83 399 24 1271 525 958 Cuts at: 4171 442 Size: 1753 Fragments arranged by size: 1753 1271 958 525 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5482 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2763 2763 Size: 5482 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4142 1239 Size: 2903 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2628 3607 4472 4770 2628 Size: 979 865 298 3340 Fragments arranged by size: 3340 979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4145 882 Size: 617 2646 2219 Fragments arranged by size: 2646 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2111 3273 493 Size: 711 907 1162 2702 Fragments arranged by size: 2702 1162 907 711 BsaBI GATnn'nnATC Cuts at: 2429 5476 2429 Size: 3047 2435 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3289 3671 4306 275 Size: 53 83 186 936 1756 382 635 1451 Fragments arranged by size: 1756 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2397 3915 4313 4587 4734 5145 2397 Size: 1518 398 274 147 411 2734 Fragments arranged by size: 2734 1518 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5257 Fragments arranged by size: 5257 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2609 1186 Size: 1423 4059 BseYI C'CCAG_C Cuts at: 2659 4685 5181 2659 Size: 2026 496 2960 Fragments arranged by size: 2960 2026 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4909 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2932 3693 4721 1540 Size: 275 186 931 761 1028 2301 Fragments arranged by size: 2301 1028 931 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2419 3543 3628 4699 2419 Size: 1124 85 1071 3202 Fragments arranged by size: 3202 1124 1071 85 BsmI GAATG_Cn' Cuts at: 2393 2993 3086 2393 Size: 600 93 4789 Fragments arranged by size: 4789 600 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2299 2419 2579 2644 2801 3543 3628 Size: 399 509 120 160 65 157 742 85 Cuts at: 3628 4699 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 509 399 160 157 120 85 65 BspEI T'CCGG_A Cuts at: 5145 5145 Size: 5482 BspHI T'CATG_A Cuts at: 2604 3371 5101 2604 Size: 767 1730 2985 Fragments arranged by size: 2985 1730 767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2837 844 Size: 1993 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2257 3369 1394 Size: 429 434 1112 3507 Fragments arranged by size: 3507 1112 434 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2567 3977 4151 65 Size: 1183 1319 1410 174 1396 Fragments arranged by size: 1410 1396 1319 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4126 4313 1700 Size: 110 2316 187 2869 Fragments arranged by size: 2869 2316 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5482 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5482 BssSI C'ACGA_G Cuts at: 3542 4554 3542 Size: 1012 4470 BstAPI GCAn_nnn'nTGC Cuts at: 794 2018 794 Size: 1224 4258 BstBI TT'CG_AA Cuts at: 4286 4286 Size: 5482 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5482 BstXI CCAn_nnnn'nTGG Cuts at: 2533 2533 Size: 5482 BstYI r'GATC_y Cuts at: 1972 2041 2812 3179 3564 3581 5033 5119 5131 Size: 69 771 367 385 17 1452 86 12 Cuts at: 5131 5477 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 771 385 367 346 86 69 17 12 Bsu36I CC'TnA_GG Cuts at: 2592 2592 Size: 5482 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4743 Fragments arranged by size: 4743 683 56 BtsI GCAGTG_nn' Cuts at: 2998 3792 3812 2998 Size: 794 20 4668 Fragments arranged by size: 4668 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5482 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5447 DraI TTT'AAA Cuts at: 2786 2786 Size: 5482 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4489 809 Size: 507 3173 1802 Fragments arranged by size: 3173 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2929 3818 5447 Size: 54 1425 50 275 18 1099 889 1629 Cuts at: 5447 8 Size: 43 Fragments arranged by size: 1629 1425 1099 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2929 1537 Size: 275 1117 4090 Fragments arranged by size: 4090 1117 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3412 1353 Size: 2059 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4069 4443 4589 387 Size: 1064 138 2480 374 146 1280 Fragments arranged by size: 2480 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 3545 4142 4929 5197 1239 Size: 393 597 1316 597 787 268 1524 Fragments arranged by size: 1524 1316 787 597 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2477 2477 Size: 5482 EcoRI G'AATT_C Cuts at: 2791 3169 2791 Size: 378 5104 EcoRV GAT'ATC Cuts at: 2203 2203 Size: 5482 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2572 2572 Size: 5482 FspI TGC'GCA Cuts at: 3988 3988 Size: 5482 HaeII r_GCGC'y Cuts at: 1678 4629 1678 Size: 2951 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4123 4155 4197 4229 1373 Size: 32 135 32 2551 32 42 32 2626 Fragments arranged by size: 2626 2551 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2826 3072 669 Size: 988 281 888 246 3079 Fragments arranged by size: 3079 988 888 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5482 HpaI GTT'AAC Cuts at: 3072 3072 Size: 5482 KpnI G_GTAC'C Cuts at: 2807 2807 Size: 5482 MfeI C'AATT_G Cuts at: 3081 3081 Size: 5482 MluI A'CGCG_T Cuts at: 5358 5358 Size: 5482 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4596 4780 1305 Size: 3291 184 2007 Fragments arranged by size: 3291 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3660 Fragments arranged by size: 3660 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2531 3440 3799 3958 518 Size: 648 1365 909 359 159 2042 Fragments arranged by size: 2042 1365 909 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2075 2898 3575 4723 4968 1606 Size: 469 823 677 1148 245 2120 Fragments arranged by size: 2120 1148 823 677 469 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5482 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5482 NotI GC'GGCC_GC Cuts at: 2929 2929 Size: 5482 NspI r_CATG'y Cuts at: 1132 1229 2016 2659 2840 4385 1132 Size: 97 787 643 181 1545 2229 Fragments arranged by size: 2229 1545 787 643 181 97 PasI CC'CwG_GG Cuts at: 1348 2473 1348 Size: 1125 4357 PciI A'CATG_T Cuts at: 4381 4381 Size: 5482 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5482 PfoI T'CCnGG_A Cuts at: 2406 2406 Size: 5482 PmeI GTTT'AAAC Cuts at: 2786 2786 Size: 5482 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2537 2569 3551 3583 5064 5096 2537 Size: 32 982 32 1481 32 2923 Fragments arranged by size: 2923 1481 982 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5482 PsiI TTA'TAA Cuts at: 3052 3052 Size: 5482 PstI C_TGCA'G Cuts at: 830 2239 2772 2834 830 Size: 1409 533 62 3478 Fragments arranged by size: 3478 1409 533 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5482 PvuII CAG'CTG Cuts at: 2075 2075 Size: 5482 SalI G'TCGA_C Cuts at: 1936 2824 1936 Size: 888 4594 SbfI CC_TGCA'GG Cuts at: 2834 2834 Size: 5482 ScaI AGT'ACT Cuts at: 3730 5442 3730 Size: 1712 3770 SfcI C'TryA_G Cuts at: 826 1045 2235 2768 2830 3965 4646 4837 826 Size: 219 1190 533 62 1135 681 191 1471 Fragments arranged by size: 1471 1190 1135 681 533 219 191 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5482 SmaI CCC'GGG Cuts at: 1872 2809 1872 Size: 937 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2643 3586 4487 4749 820 Size: 197 926 700 943 901 262 1553 Fragments arranged by size: 1553 943 926 901 700 262 197 SnaBI TAC'GTA Cuts at: 493 3273 493 Size: 2780 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5482 SphI G_CATG'C Cuts at: 1132 2016 2659 2840 1132 Size: 884 643 181 3774 Fragments arranged by size: 3774 884 643 181 SspI AAT'ATT Cuts at: 5 52 2220 3406 5 Size: 47 2168 1186 2081 Fragments arranged by size: 2168 2081 1186 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5482 StyI C'CwwG_G Cuts at: 513 1065 2004 2921 513 Size: 552 939 917 3074 Fragments arranged by size: 3074 939 917 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3509 3526 3679 3864 4309 325 Size: 1255 1929 17 153 185 445 1498 Fragments arranged by size: 1929 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3728 5440 96 Size: 274 80 33 51 1443 1751 1712 138 Fragments arranged by size: 1751 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2475 2507 5442 5474 2475 Size: 32 2935 32 2483 Fragments arranged by size: 2935 2483 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5482 XbaI T'CTAG_A Cuts at: 2818 2818 Size: 5482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5482 XmaI C'CCGG_G Cuts at: 1870 2807 1870 Size: 937 4545 XmnI GAAnn'nnTTC Cuts at: 2367 2552 3611 2367 Size: 185 1059 4238 Fragments arranged by size: 4238 1059 185 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3290 276 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI