Restriction map for
pFN21AE0106
(Circular) (Six-base) MAPSORT of: pFN21AE0106.seq Check: 6644 from: 1 to: 5548
pFN21AE0106 5548 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2891 1937
Size: 954 4594
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2384 3611 4995 5263 1632
Size: 752 1227 1384 268 1917
Fragments arranged by size:
1917 1384 1227 752 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5548
AlwNI CAG_nnn'CTG
Cuts at: 2130 2278 2428 2743 3291 4863 2130
Size: 148 150 315 548 1572 2815
Fragments arranged by size:
2815 1572 548 315 150 148
ApaI G_GGCC'C
Cuts at: 2634 2634
Size: 5548
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2720 2857 3083 3235 5344 1493
Size: 1227 137 226 152 2109 1697
Fragments arranged by size:
2109 1697 1227 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2873 1870
Size: 73 930 4545
Fragments arranged by size:
4545 930 73
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2330 2537 2869 4324 618
Size: 325 217 627 543 207 332 1455 1842
Fragments arranged by size:
1842 1455 627 543 332 325 217 207
BanII G_rGCy'C
Cuts at: 1391 2236 2634 2867 1391
Size: 845 398 233 4072
Fragments arranged by size:
4072 845 398 233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2462 928
Size: 971 563 4014
Fragments arranged by size:
4014 971 563
BbvCI CC'TCA_GC
Cuts at: 2608 2608
Size: 5548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3739 3773 1903
Size: 34 1802 34 3678
Fragments arranged by size:
3678 1802 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4159 136
Size: 107 122 71 631 605 2487 1525
Fragments arranged by size:
2487 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5543 5543
Size: 5548
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 2634 3609 4765 1790
Size: 844 975 1156 2573
Fragments arranged by size:
2573 1156 975 844
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2266 3279 4237
Size: 416 53 83 399 24 849 1013 958
Cuts at: 4237 442
Size: 1753
Fragments arranged by size:
1753 1013 958 849 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2792 4208 1239
Size: 888 665 1416 2579
Fragments arranged by size:
2579 1416 888 665
Bpu10I CC'TnA_GC
Cuts at: 2240 2608 2240
Size: 368 5180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2303 2738 3673 4538 4836 2303
Size: 435 935 865 298 3015
Fragments arranged by size:
3015 935 865 435 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2032 2220 4211 882
Size: 617 533 188 1991 2219
Fragments arranged by size:
2219 1991 617 533 188
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2111 2371 3355 3737
Size: 53 83 186 936 578 260 984 382
Cuts at: 3737 4372 275
Size: 635 1451
Fragments arranged by size:
1451 984 936 635 578 382 260 186
83 53
BsaWI w'CCGG_w
Cuts at: 3981 4379 4653 4800 5211 3981
Size: 398 274 147 411 4318
Fragments arranged by size:
4318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2130 1186
Size: 944 4604
BseYI C'CCAG_C
Cuts at: 2612 2665 4751 5247 2612
Size: 53 2086 496 2913
Fragments arranged by size:
2913 2086 496 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 1163
Size: 573 528 4447
Fragments arranged by size:
4447 573 528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2295 2998 3759 4787 1540
Size: 275 186 294 703 761 1028 2301
Fragments arranged by size:
2301 1028 761 703 294 275 186
BsiHKAI G_wGCw'C
Cuts at: 2162 2690 2778 3609 3694 4765 2162
Size: 528 88 831 85 1071 2945
Fragments arranged by size:
2945 1071 831 528 88 85
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2236 2634 2690 2778 2867 3609
Size: 399 372 74 398 56 88 89 742
Cuts at: 3609 3694 4765 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 372 89 88
85 74 56
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 2903 844
Size: 1463 596 3489
Fragments arranged by size:
3489 1463 596
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4043 4217 65
Size: 1183 2795 174 1396
Fragments arranged by size:
2795 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2253 4192 4379 1700
Size: 110 443 1939 187 2869
Fragments arranged by size:
2869 1939 443 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5548
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2743 2782 794
Size: 1949 39 3560
Fragments arranged by size:
3560 1949 39
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstXI CCAn_nnnn'nTGG
Cuts at: 2840 2840
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2553 2878 3245 3630 3647 5099 5185 5197
Size: 581 325 367 385 17 1452 86 12
Cuts at: 5197 5543 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 581 385 367 346 325 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2033 513
Size: 552 968 4028
Fragments arranged by size:
4028 968 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 2362 3064 3858 3878 2362
Size: 702 794 20 4032
Fragments arranged by size:
4032 794 702 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2625 2660 529
Size: 35 2061 35 3417
Fragments arranged by size:
3417 2061 35 35
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DraIII CAC_nnn'GTG
Cuts at: 2782 2782
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2292 2437 2523
Size: 54 1425 50 275 18 462 145 86
Cuts at: 2523 2842 2995 3884 5513 8
Size: 319 153 889 1629 43
Fragments arranged by size:
1629 1425 889 462 319 275 153 145
86 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2292 2995 1537
Size: 275 480 703 4090
Fragments arranged by size:
4090 703 480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2487 3478 1353
Size: 1134 991 3423
Fragments arranged by size:
3423 1134 991
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4509 4655 387
Size: 1064 138 2546 374 146 1280
Fragments arranged by size:
2546 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2384 2792 3611 4208 4995 5263
Size: 393 495 257 408 819 597 787 268
Cuts at: 5263 1239
Size: 1524
Fragments arranged by size:
1524 819 787 597 495 408 393 268
257
EcoNI CCTnn'n_nnAGG
Cuts at: 2145 2282 2378 2145
Size: 137 96 5315
Fragments arranged by size:
5315 137 96
EcoO109I rG'GnC_Cy
Cuts at: 2425 2596 2617 2631 2425
Size: 171 21 14 5342
Fragments arranged by size:
5342 171 21 14
EcoRI G'AATT_C
Cuts at: 2720 2857 3235 2720
Size: 137 378 5033
Fragments arranged by size:
5033 378 137
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 2254 4695 1678
Size: 576 2441 2531
Fragments arranged by size:
2531 2441 576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2464 2496 4189 4221 4263
Size: 32 135 32 892 32 1693 32 42
Cuts at: 4263 4295 1373
Size: 32 2626
Fragments arranged by size:
2626 1693 892 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2491 2530 2892 3138 669
Size: 988 281 553 39 362 246 3079
Fragments arranged by size:
3079 988 553 362 281 246 39
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2030 2634 4662 4846 1305
Size: 725 604 2028 184 2007
Fragments arranged by size:
2028 2007 725 604 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2439 2844 10
Size: 54 1425 343 607 405 2714
Fragments arranged by size:
2714 1425 607 405 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3506 3865 4024 518
Size: 648 2340 359 159 2042
Fragments arranged by size:
2340 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2308 2512 2612 2665 2964 3641 4789 5034
Size: 702 204 100 53 299 677 1148 245
Cuts at: 5034 1606
Size: 2120
Fragments arranged by size:
2120 1148 702 677 299 245 204 100
53
NaeI GCC'GGC
Cuts at: 2255 2255
Size: 5548
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4996
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NgoMIV G'CCGG_C
Cuts at: 2253 2253
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2906 4451 1132
Size: 97 1677 1545 2229
Fragments arranged by size:
2229 1677 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2627 1348
Size: 1279 4269
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5548
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2425 2596 2617 2425
Size: 171 21 5356
Fragments arranged by size:
5356 171 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PspOMI G'GGCC_C
Cuts at: 2630 2630
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2066 2739 2900 830
Size: 1236 673 161 3478
Fragments arranged by size:
3478 1236 673 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
PvuII CAG'CTG
Cuts at: 2512 2612 2665 2512
Size: 100 53 5395
Fragments arranged by size:
5395 100 53
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SapI GCTCTTCn'nnn_
Cuts at: 2487 2487
Size: 5548
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SfcI C'TryA_G
Cuts at: 826 1045 2062 2660 2735 2896 4031 4712 4903
Size: 219 1017 598 75 161 1135 681 191
Cuts at: 4903 826
Size: 1471
Fragments arranged by size:
1471 1135 1017 681 598 219 191 161
75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2875 1872
Size: 1003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2318 2753 3652 4553 4815 820
Size: 197 926 375 435 899 901 262 1553
Fragments arranged by size:
1553 926 901 899 435 375 262 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5548
StyI C'CwwG_G
Cuts at: 513 1065 2337 2987 513
Size: 552 1272 650 3074
Fragments arranged by size:
3074 1272 650 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2244 2285 2555 3575 3592 3745 3930
Size: 1255 664 41 270 1020 17 153 185
Cuts at: 3930 4375 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1020 664 445 270 185 153
41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3794 5506 96
Size: 274 80 33 51 1443 1817 1712 138
Fragments arranged by size:
1817 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5508 5540 5508
Size: 32 5516
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2873 1870
Size: 1003 4545
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI