Restriction map for
pFN21AE0098
(Circular) (Six-base) MAPSORT of: pFN21AE0098.seq Check: 5480 from: 1 to: 5803
pFN21AE0098 5803 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2059 3866 5250 5518 1632
Size: 427 1807 1384 268 1917
Fragments arranged by size:
1917 1807 1384 427 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 4702 5679 1203
Size: 3499 977 1327
Fragments arranged by size:
3499 1327 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5803
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2567 2599 2567
Size: 32 5771
AlwNI CAG_nnn'CTG
Cuts at: 2035 3546 5118 2035
Size: 1511 1572 2720
Fragments arranged by size:
2720 1572 1511
ApaI G_GGCC'C
Cuts at: 2655 2655
Size: 5803
ApaLI G'TGCA_C
Cuts at: 3860 5016 3860
Size: 1156 4647
ApoI r'AATT_y
Cuts at: 1493 3112 3338 3490 5599 1493
Size: 1619 226 152 2109 1697
Fragments arranged by size:
2109 1697 1619 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2648 2689 3128 1870
Size: 73 105 600 41 439 4545
Fragments arranged by size:
4545 600 439 105 73 41
BamHI G'GATC_C
Cuts at: 2399 3133 3500 2399
Size: 734 367 4702
Fragments arranged by size:
4702 734 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2796 2858 3124 4579 618
Size: 325 217 627 1009 62 266 1455 1842
Fragments arranged by size:
1842 1455 1009 627 325 266 217 62
BanII G_rGCy'C
Cuts at: 1391 2649 2655 3122 1391
Size: 1258 6 467 4072
Fragments arranged by size:
4072 1258 467 6
BbeI G_GCGC'C
Cuts at: 2862 2862
Size: 5803
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4832
BbvCI CC'TCA_GC
Cuts at: 2211 2211
Size: 5803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2955 2989 3994 4028 1903
Size: 34 50 34 934 34 1005 34 3678
Fragments arranged by size:
3678 1005 934 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4414 136
Size: 107 122 71 631 605 2742 1525
Fragments arranged by size:
2742 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2736 5798 2736
Size: 3062 2741
BlpI GC'TnA_GC
Cuts at: 3220 3220
Size: 5803
Bme1580I G_kGCm'C
Cuts at: 1790 2655 2879 3864 5020 1790
Size: 865 224 985 1156 2573
Fragments arranged by size:
2573 1156 985 865 224
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2393 3534 4492
Size: 416 53 83 399 24 976 1141 958
Cuts at: 4492 442
Size: 1753
Fragments arranged by size:
1753 1141 976 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2325 2469 2651 2770 2982 2999 4463 1239
Size: 1086 144 182 119 212 17 1464 2579
Fragments arranged by size:
2579 1464 1086 212 182 144 119 17
Bpu10I CC'TnA_GC
Cuts at: 2211 2211
Size: 5803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2009 2129 2612 3928 4793 5091 2009
Size: 120 483 1316 865 298 2721
Fragments arranged by size:
2721 1316 865 483 298 120
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4466 882
Size: 617 2967 2219
Fragments arranged by size:
2967 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3594 493
Size: 711 2390 2702
Fragments arranged by size:
2702 2390 711
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2292 2859 3610 3992
Size: 53 83 186 936 759 567 751 382
Cuts at: 3992 4627 275
Size: 635 1451
Fragments arranged by size:
1451 936 759 751 635 567 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2443 4236 4634 4908 5055 5466 2443
Size: 1793 398 274 147 411 2780
Fragments arranged by size:
2780 1793 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2997 3027 1178
Size: 30 165 30 1594 30 3954
Fragments arranged by size:
3954 1594 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2537 2540 1186
Size: 1351 3 4449
Fragments arranged by size:
4449 1351 3
BseYI C'CCAG_C
Cuts at: 2435 2586 2674 2930 5006 5502 2435
Size: 151 88 256 2076 496 2736
Fragments arranged by size:
2736 2076 496 256 151 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 1163
Size: 573 387 4843
Fragments arranged by size:
4843 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3253 4014 5042 1540
Size: 275 186 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 2557 3864 3949 5020 2118
Size: 439 1307 85 1071 2901
Fragments arranged by size:
2901 1307 1071 439 85
BsiWI C'GTAC_G
Cuts at: 3099 3099
Size: 5803
BsmI GAATG_Cn'
Cuts at: 2439 3314 3407 2439
Size: 875 93 4835
Fragments arranged by size:
4835 875 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2796 2796
Size: 5803
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2557 2649 2655 2879 3122 3864
Size: 399 328 439 92 6 224 243 742
Cuts at: 3864 3949 5020 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 328 243 224
92 85 6
BspEI T'CCGG_A
Cuts at: 2443 5466 2443
Size: 3023 2780
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3158 844
Size: 2314 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2749 4298 4472 65
Size: 1183 1501 1549 174 1396
Fragments arranged by size:
1549 1501 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2790 4447 4634 1700
Size: 110 980 1657 187 2869
Fragments arranged by size:
2869 1657 980 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 3863 4875 3863
Size: 1012 4791
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2035 794
Size: 1241 4562
BstBI TT'CG_AA
Cuts at: 4607 4607
Size: 5803
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5803
BstXI CCAn_nnnn'nTGG
Cuts at: 2129 2911 2938 2129
Size: 782 27 4994
Fragments arranged by size:
4994 782 27
BstYI r'GATC_y
Cuts at: 1972 2031 2308 2399 2736 3133 3500 3885 3902
Size: 59 277 91 337 397 367 385 17
Cuts at: 3902 5354 5440 5452 5798 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 397 385 367 346 337 277
91 86 59 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2091 2370 513
Size: 552 1026 279 3946
Fragments arranged by size:
3946 1026 552 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5064
Fragments arranged by size:
5064 683 56
BtsI GCAGTG_nn'
Cuts at: 2958 3319 4113 4133 2958
Size: 361 794 20 4628
Fragments arranged by size:
4628 794 361 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2811 3250 4139
Size: 54 1425 50 275 18 981 439 889
Cuts at: 4139 5768 8
Size: 1629 43
Fragments arranged by size:
1629 1425 981 889 439 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3250 1537
Size: 275 1438 4090
Fragments arranged by size:
4090 1438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 2514 3015 3095 3733 1353
Size: 725 436 501 80 638 3423
Fragments arranged by size:
3423 725 638 501 436 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4390 4764 4910 387
Size: 1064 138 2801 374 146 1280
Fragments arranged by size:
2801 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2325 2469 2651 2770 2982 2999
Size: 393 427 266 144 182 119 212 17
Cuts at: 2999 3866 4463 5250 5518 1239
Size: 867 597 787 268 1524
Fragments arranged by size:
1524 867 787 597 427 393 268 266
212 182 144 119 17
EcoO109I rG'GnC_Cy
Cuts at: 2135 2678 2135
Size: 543 5260
EcoRI G'AATT_C
Cuts at: 3112 3490 3112
Size: 378 5425
FspI TGC'GCA
Cuts at: 3075 4309 3075
Size: 1234 4569
HaeII r_GCGC'y
Cuts at: 1678 2862 4950 1678
Size: 1184 2088 2531
Fragments arranged by size:
2531 2088 1184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2127 2159 3012 3044 3072
Size: 32 135 32 555 32 853 32 28
Cuts at: 3072 3104 4444 4476 4518 4550 1373
Size: 32 1340 32 42 32 2626
Fragments arranged by size:
2626 1340 853 555 135 42 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2755 3147 3393 669
Size: 988 281 817 392 246 3079
Fragments arranged by size:
3079 988 817 392 281 246
HindIII A'AGCT_T
Cuts at: 748 2661 748
Size: 1913 3890
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KasI G'GCGC_C
Cuts at: 2858 2858
Size: 5803
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2791 4917 5101 1305
Size: 1486 2126 184 2007
Fragments arranged by size:
2126 2007 1486 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3981
Fragments arranged by size:
3981 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3761 4120 4279 518
Size: 648 2595 359 159 2042
Fragments arranged by size:
2595 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2963 2980 3219 3896 5044 5289 1606
Size: 1357 17 239 677 1148 245 2120
Fragments arranged by size:
2120 1357 1148 677 245 239 17
NaeI GCC'GGC
Cuts at: 2792 2792
Size: 5803
NarI GG'CG_CC
Cuts at: 2859 2859
Size: 5803
NcoI C'CATG_G
Cuts at: 513 1065 2091 2370 513
Size: 552 1026 279 3946
Fragments arranged by size:
3946 1026 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NgoMIV G'CCGG_C
Cuts at: 2790 2790
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 3161 4706 1132
Size: 97 1932 1545 2229
Fragments arranged by size:
2229 1932 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5803
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2339 1108
Size: 1231 4572
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 5385 5417 3872
Size: 32 1481 32 4258
Fragments arranged by size:
4258 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2135 2135
Size: 5803
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PspOMI G'GGCC_C
Cuts at: 2651 2651
Size: 5803
PspXI vC'TCGA_Gb
Cuts at: 2048 2048
Size: 5803
PstI C_TGCA'G
Cuts at: 830 2953 3155 830
Size: 2123 202 3478
Fragments arranged by size:
3478 2123 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SanDI GG'GwC_CC
Cuts at: 2135 2135
Size: 5803
SbfI CC_TGCA'GG
Cuts at: 3155 3155
Size: 5803
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SfcI C'TryA_G
Cuts at: 826 1045 2083 2949 3151 4286 4967 5158 826
Size: 219 1038 866 202 1135 681 191 1471
Fragments arranged by size:
1471 1135 1038 866 681 219 202 191
SfoI GGC'GCC
Cuts at: 2860 2860
Size: 5803
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 2691 3130 1872
Size: 819 439 4545
Fragments arranged by size:
4545 819 439
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2024 2048 2144 2591 3907 4808
Size: 197 926 81 24 96 447 1316 901
Cuts at: 4808 5070 820
Size: 262 1553
Fragments arranged by size:
1553 1316 926 901 447 262 197 96
81 24
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 3161 1132
Size: 2029 3774
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5803
StyI C'CwwG_G
Cuts at: 513 1065 2065 2091 2370 2466 2892 3242 513
Size: 552 1000 26 279 96 426 350 3074
Fragments arranged by size:
3074 1000 552 426 350 279 96 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2733 3830 3847 4000 4185 4630 325
Size: 1255 1153 1097 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1153 1097 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4049 5761 96
Size: 274 80 33 51 1443 2072 1712 138
Fragments arranged by size:
2072 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2997 3029 5763 5795 2997
Size: 32 2734 32 3005
Fragments arranged by size:
3005 2734 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XhoI C'TCGA_G
Cuts at: 1943 2048 1943
Size: 105 5698
XmaI C'CCGG_G
Cuts at: 1870 2689 3128 1870
Size: 819 439 4545
Fragments arranged by size:
4545 819 439
XmnI GAAnn'nnTTC
Cuts at: 3932 3932
Size: 5803
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SrfI SwaI XcmI