Restriction map for
pFN21AE0097
(Circular) (Six-base) MAPSORT of: pFN21AE0097.seq Check: 8248 from: 1 to: 5554
pFN21AE0097 5554 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3364 278
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 1937
Size: 960 4594
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3617 5001 5269 1632
Size: 1985 1384 268 1917
Fragments arranged by size:
1985 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 2270 2514 2611 4453 5430 1203
Size: 1067 244 97 1842 977 1327
Fragments arranged by size:
1842 1327 1067 977 244 97
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 4283 4283
Size: 5554
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5554
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2655 2670 1184
Size: 1471 15 4068
Fragments arranged by size:
4068 1471 15
AlwNI CAG_nnn'CTG
Cuts at: 3297 4869 3297
Size: 1572 3982
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2300 2384 2420 2731 2863 3089 3241 5350
Size: 807 84 36 311 132 226 152 2109
Cuts at: 5350 1493
Size: 1697
Fragments arranged by size:
2109 1697 807 311 226 152 132 84
36
AseI AT'TA_AT
Cuts at: 160 1032 2258 4108 160
Size: 872 1226 1850 1606
Fragments arranged by size:
1850 1606 1226 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2879 1870
Size: 73 936 4545
Fragments arranged by size:
4545 936 73
BamHI G'GATC_C
Cuts at: 2884 3251 2884
Size: 367 5187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2875 4330 618
Size: 325 217 627 1088 1455 1842
Fragments arranged by size:
1842 1455 1088 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2030 2873 1391
Size: 639 843 4072
Fragments arranged by size:
4072 843 639
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2517 928
Size: 971 618 3965
Fragments arranged by size:
3965 971 618
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 1903
Size: 34 1808 34 3678
Fragments arranged by size:
3678 1808 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2589 3446 4656 2589
Size: 857 1210 3487
Fragments arranged by size:
3487 1210 857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4165 136
Size: 107 122 71 631 605 2493 1525
Fragments arranged by size:
2493 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2062 5549 2062
Size: 3487 2067
BlpI GC'TnA_GC
Cuts at: 2658 2971 2658
Size: 313 5241
Bme1580I G_kGCm'C
Cuts at: 1790 3615 4771 1790
Size: 1825 1156 2573
Fragments arranged by size:
2573 1825 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3285 4243 442
Size: 416 53 83 399 24 1868 958 1753
Fragments arranged by size:
1868 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 4214 1239
Size: 1229 1746 2579
Fragments arranged by size:
2579 1746 1229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3679 4544 4842 3679
Size: 865 298 4391
Fragments arranged by size:
4391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4217 882
Size: 617 2718 2219
Fragments arranged by size:
2718 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3345 493
Size: 711 2141 2702
Fragments arranged by size:
2702 2141 711
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3361 3743 4378 275
Size: 53 83 186 936 1828 382 635 1451
Fragments arranged by size:
1828 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3987 4385 4659 4806 5217 3987
Size: 398 274 147 411 4324
Fragments arranged by size:
4324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2466 2496 1178
Size: 30 165 30 1063 30 4236
Fragments arranged by size:
4236 1063 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5554
BseYI C'CCAG_C
Cuts at: 4757 5253 4757
Size: 496 5058
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2513 1163
Size: 573 777 4204
Fragments arranged by size:
4204 777 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3004 3765 4793 1540
Size: 275 186 1003 761 1028 2301
Fragments arranged by size:
2301 1028 1003 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 3615 3700 4771 2030
Size: 1585 85 1071 2813
Fragments arranged by size:
2813 1585 1071 85
BsmI GAATG_Cn'
Cuts at: 3065 3158 3065
Size: 93 5461
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2873 3615 3700 4771 1391
Size: 399 240 843 742 85 1071 2174
Fragments arranged by size:
2174 1071 843 742 399 240 85
BspEI T'CCGG_A
Cuts at: 5217 5217
Size: 5554
BspHI T'CATG_A
Cuts at: 2589 3443 5173 2589
Size: 854 1730 2970
Fragments arranged by size:
2970 1730 854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2909 844
Size: 2065 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 1394
Size: 429 1618 3507
Fragments arranged by size:
3507 1618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2048 2654 4049 4223 65
Size: 1183 800 606 1395 174 1396
Fragments arranged by size:
1396 1395 1183 800 606 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4198 4385 1700
Size: 110 2388 187 2869
Fragments arranged by size:
2869 2388 187 110
BsrGI T'GTAC_A
Cuts at: 96 2626 96
Size: 2530 3024
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5554
BssSI C'ACGA_G
Cuts at: 3614 4626 3614
Size: 1012 4542
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5554
BstBI TT'CG_AA
Cuts at: 4358 4358
Size: 5554
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstYI r'GATC_y
Cuts at: 1972 2062 2248 2563 2884 3251 3636 3653 5105
Size: 90 186 315 321 367 385 17 1452
Cuts at: 5105 5191 5203 5549 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 321 315 186
90 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4063
Fragments arranged by size:
4063 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4815
Fragments arranged by size:
4815 683 56
BtsI GCAGTG_nn'
Cuts at: 2193 2729 3070 3864 3884 2193
Size: 536 341 794 20 3863
Fragments arranged by size:
3863 794 536 341 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2091 2709 2858 2091
Size: 618 149 4787
Fragments arranged by size:
4787 618 149
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4561 809
Size: 507 3245 1802
Fragments arranged by size:
3245 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3001 3890 5519
Size: 54 1425 50 275 18 1171 889 1629
Cuts at: 5519 8
Size: 43
Fragments arranged by size:
1629 1425 1171 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 1537
Size: 275 1189 4090
Fragments arranged by size:
4090 1189 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3484 1353
Size: 2131 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4141 4515 4661 387
Size: 1064 138 2552 374 146 1280
Fragments arranged by size:
2552 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2028 2028
Size: 5554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 3617 4214 5001 5269 1239
Size: 393 836 1149 597 787 268 1524
Fragments arranged by size:
1524 1149 836 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2420 2863 3241 2420
Size: 443 378 4733
Fragments arranged by size:
4733 443 378
FspI TGC'GCA
Cuts at: 4060 4060
Size: 5554
HaeII r_GCGC'y
Cuts at: 1678 2080 4701 1678
Size: 402 2621 2531
Fragments arranged by size:
2621 2531 402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2446 2466 2478 2498 4195
Size: 32 135 32 874 20 12 20 1697
Cuts at: 4195 4227 4269 4301 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1697 874 135 42 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4668 4852 1305
Size: 3363 184 2007
Fragments arranged by size:
3363 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3732
Fragments arranged by size:
3732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3512 3871 4030 518
Size: 648 2346 359 159 2042
Fragments arranged by size:
2346 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2970 3647 4795 5040 1606
Size: 1364 677 1148 245 2120
Fragments arranged by size:
2120 1364 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4063
Fragments arranged by size:
4063 939 552
NdeI CA'TA_TG
Cuts at: 387 2368 387
Size: 1981 3573
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2274 2518 2615 2912 4457 1132
Size: 97 1045 244 97 297 1545 2229
Fragments arranged by size:
2229 1545 1045 297 244 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5554
PciI A'CATG_T
Cuts at: 2270 2514 2611 4453 2270
Size: 244 97 1842 3371
Fragments arranged by size:
3371 1842 244 97
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2649 1108
Size: 1541 4013
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 5136 5168 3623
Size: 32 1481 32 4009
Fragments arranged by size:
4009 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5554
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 5522
PstI C_TGCA'G
Cuts at: 830 2906 830
Size: 2076 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
SacI G_AGCT'C
Cuts at: 2030 2030
Size: 5554
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 3802 5514 3802
Size: 1712 3842
SfcI C'TryA_G
Cuts at: 826 1045 2554 2842 2902 4037 4718 4909 826
Size: 219 1509 288 60 1135 681 191 1471
Fragments arranged by size:
1509 1471 1135 681 288 219 191 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2881 1872
Size: 1009 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3658 4559 4821 820
Size: 197 926 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SspI AAT'ATT
Cuts at: 5 52 2114 2130 3478 5
Size: 47 2062 16 1348 2081
Fragments arranged by size:
2081 2062 1348 47 16
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5554
StyI C'CwwG_G
Cuts at: 513 1065 2004 2993 513
Size: 552 939 989 3074
Fragments arranged by size:
3074 989 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3581 3598 3751 3936 4381 325
Size: 1255 2001 17 153 185 445 1498
Fragments arranged by size:
2001 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2626 3800 5512
Size: 274 80 33 51 1443 649 1174 1712
Cuts at: 5512 96
Size: 138
Fragments arranged by size:
1712 1443 1174 649 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5514 5546 5514
Size: 32 5522
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5554
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2879 1870
Size: 1009 4545
XmnI GAAnn'nnTTC
Cuts at: 2732 3683 2732
Size: 951 4603
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3362 276
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI