Restriction map for
pFN21AE0095
(Circular) (Six-base) MAPSORT of: pFN21AE0095.seq Check: 3514 from: 1 to: 8488
pFN21AE0095 8488 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5014 5014
Size: 8488
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3756 6298 278
Size: 53 83 186 936 2220 2542 2468
Fragments arranged by size:
2542 2468 2220 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4275 5831 1937
Size: 2338 1556 4594
Fragments arranged by size:
4594 2338 1556
Acc65I G'GTAC_C
Cuts at: 5809 5809
Size: 8488
AclI AA'CG_TT
Cuts at: 1563 6615 6988 1563
Size: 5052 373 3063
Fragments arranged by size:
5052 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2272 3152 3606 4219 6551 7935 8203 1632
Size: 640 880 454 613 2332 1384 268 1917
Fragments arranged by size:
2332 1917 1384 880 640 613 454 268
AfeI AGC'GCT
Cuts at: 2699 2699
Size: 8488
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8291
AflIII A'CryG_T
Cuts at: 1203 4560 4635 5673 5697 7387 8364 1203
Size: 3357 75 1038 24 1690 977 1327
Fragments arranged by size:
3357 1690 1327 1038 977 75 24
AgeI A'CCGG_T
Cuts at: 5639 7319 5639
Size: 1680 6808
AhdI GACnn_n'nnGTC
Cuts at: 3757 7217 3757
Size: 3460 5028
AleI CACnn'nnGTG
Cuts at: 1166 3612 3720 4056 4349 4535 1166
Size: 2446 108 336 293 186 5119
Fragments arranged by size:
5119 2446 336 293 186 108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2070 1184
Size: 886 7602
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 8456
AlwNI CAG_nnn'CTG
Cuts at: 2209 2220 3127 3472 4704 5686 6231 7803 2209
Size: 11 907 345 1232 982 545 1572 2894
Fragments arranged by size:
2894 1572 1232 982 907 545 345 11
ApaI G_GGCC'C
Cuts at: 2601 5778 2601
Size: 3177 5311
ApaLI G'TGCA_C
Cuts at: 3457 6545 7701 3457
Size: 3088 1156 4244
Fragments arranged by size:
4244 3088 1156
ApoI r'AATT_y
Cuts at: 1493 3696 5797 6023 6175 8284 1493
Size: 2203 2101 226 152 2109 1697
Fragments arranged by size:
2203 2109 2101 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7042 160
Size: 872 6010 1606
Fragments arranged by size:
6010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8488
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2070 2141 2174 2388 2581 2587
Size: 73 89 38 71 33 214 193 6
Cuts at: 2587 3237 3574 4724 5813 1870
Size: 650 337 1150 1089 4545
Fragments arranged by size:
4545 1150 1089 650 337 214 193 89
73 71 38 33 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5628 5661 5628
Size: 33 8455
BamHI G'GATC_C
Cuts at: 5818 6185 5818
Size: 367 8121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2065 2392 2531 2621
Size: 325 217 627 272 6 327 139 90
Cuts at: 2621 4289 4849 5809 7264 618
Size: 1668 560 960 1455 1842
Fragments arranged by size:
1842 1668 1455 960 627 560 327 325
272 217 139 90 6
BanII G_rGCy'C
Cuts at: 1391 2030 2150 2588 2601 3495 4827 5190 5541
Size: 639 120 438 13 894 1332 363 351
Cuts at: 5541 5778 5807 1391
Size: 237 29 4072
Fragments arranged by size:
4072 1332 894 639 438 363 351 237
120 29 13
BbeI G_GCGC'C
Cuts at: 2063 2069 2396 2535 2625 2063
Size: 6 327 139 90 7926
Fragments arranged by size:
7926 327 139 90 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2057 4107 4309 928
Size: 971 158 2050 202 5107
Fragments arranged by size:
5107 2050 971 202 158
BbvCI CC'TCA_GC
Cuts at: 3204 3204
Size: 8488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3157 3191 3699 3733 6679 6713 1903
Size: 34 1220 34 508 34 2946 34 3678
Fragments arranged by size:
3678 2946 1220 508 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6380 7590 6380
Size: 1210 7278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2034 2825 4298
Size: 107 122 71 631 605 362 791 1473
Cuts at: 4298 7099 136
Size: 2801 1525
Fragments arranged by size:
2801 1525 1473 791 631 605 362 122
107 71
BglII A'GATC_T
Cuts at: 8483 8483
Size: 8488
BlpI GC'TnA_GC
Cuts at: 2252 3059 4032 4373 4751 5905 2252
Size: 807 973 341 378 1154 4835
Fragments arranged by size:
4835 1154 973 807 378 341
Bme1580I G_kGCm'C
Cuts at: 1790 2601 3461 4011 4356 4937 5578 5778 6549
Size: 811 860 550 345 581 641 200 771
Cuts at: 6549 7705 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 860 811 771 641 581 550
345 200
BmgBI CAC'GTC
Cuts at: 1318 4213 5146 1318
Size: 2895 933 4660
Fragments arranged by size:
4660 2895 933
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3448 4033 4924
Size: 416 53 83 399 24 2031 585 891
Cuts at: 4924 6219 7177 442
Size: 1295 958 1753
Fragments arranged by size:
2031 1753 1295 958 891 585 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3779 1056
Size: 2723 5765
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3664 3664
Size: 8488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 2543 2702 2786 4459 4561 5285 5381
Size: 1265 39 159 84 1673 102 724 96
Cuts at: 5381 5729 7148 1239
Size: 348 1419 2579
Fragments arranged by size:
2579 1673 1419 1265 724 348 159 102
96 84 39
Bpu10I CC'TnA_GC
Cuts at: 3204 4938 3204
Size: 1734 6754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3884 6613 7478 7776 3884
Size: 2729 865 298 4596
Fragments arranged by size:
4596 2729 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3214 3227 3737 3911 7151 882
Size: 617 1715 13 510 174 3240 2219
Fragments arranged by size:
3240 2219 1715 617 510 174 13
BsaAI yAC'GTr
Cuts at: 493 1204 3539 4569 4645 4834 5700 6279 493
Size: 711 2335 1030 76 189 866 579 2702
Fragments arranged by size:
2702 2335 1030 866 711 579 189 76
BsaBI GATnn'nnATC
Cuts at: 2849 3386 4346 5531 8482 2849
Size: 537 960 1185 2951 2855
Fragments arranged by size:
2951 2855 1185 960 537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 2066 2393 2532
Size: 53 83 186 936 527 6 327 139
Cuts at: 2532 2622 3449 3753 4179 4973 6295 6677 7312
Size: 90 827 304 426 794 1322 382 635
Cuts at: 7312 275
Size: 1451
Fragments arranged by size:
1451 1322 936 827 794 635 527 426
382 327 304 186 139 90 83 53
6
BsaWI w'CCGG_w
Cuts at: 5639 6921 7319 7593 7740 8151 5639
Size: 1282 398 274 147 411 5976
Fragments arranged by size:
5976 1282 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2675 2705 4910 4940 1178
Size: 30 165 30 1272 30 2205 30 4726
Fragments arranged by size:
4726 2205 1272 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2501 5090 5099 5108 1186
Size: 1315 2589 9 9 4566
Fragments arranged by size:
4566 2589 1315 9 9
BseYI C'CCAG_C
Cuts at: 4588 5019 7691 8187 4588
Size: 431 2672 496 4889
Fragments arranged by size:
4889 2672 496 431
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3151 3172 3447 3922 4997 5108 1163
Size: 573 1415 21 275 475 1075 111 4543
Fragments arranged by size:
4543 1415 1075 573 475 275 111 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2341 2514 2832 4282 4659 5653
Size: 275 186 340 173 318 1450 377 994
Cuts at: 5653 5938 6699 7727 1540
Size: 285 761 1028 2301
Fragments arranged by size:
2301 1450 1028 994 761 377 340 318
285 275 186 173
BsiHKAI G_wGCw'C
Cuts at: 2757 3060 3072 3461 3495 3909 4212 4611 4809
Size: 303 12 389 34 414 303 399 198
Cuts at: 4809 4827 4839 5190 5508 6549 6634 7705 2757
Size: 18 12 351 318 1041 85 1071 3540
Fragments arranged by size:
3540 1071 1041 414 399 389 351 318
303 303 198 85 34 18 12 12
BsmI GAATG_Cn'
Cuts at: 5999 6092 5999
Size: 93 8395
BsmBI CGTCTCn'nnnn_
Cuts at: 3577 3577
Size: 8488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2150 2588 2601 2757 3060 3072
Size: 399 240 120 438 13 156 303 12
Cuts at: 3072 3461 3495 3909 4011 4212 4356 4611 4809
Size: 389 34 414 102 201 144 255 198
Cuts at: 4809 4827 4839 4937 5190 5508 5541 5578 5778
Size: 18 12 98 253 318 33 37 200
Cuts at: 5778 5807 6549 6634 7705 1391
Size: 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 438 414 399 389 318
303 255 253 240 201 200 198 156
144 120 102 98 85 37 34 33
29 18 13 12 12
BspEI T'CCGG_A
Cuts at: 8151 8151
Size: 8488
BspHI T'CATG_A
Cuts at: 3477 6377 8107 3477
Size: 2900 1730 3858
Fragments arranged by size:
3858 2900 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4261 4593 4890 5014 5019 5843 844
Size: 3417 332 297 124 5 824 3489
Fragments arranged by size:
3489 3417 824 332 297 124 5
BsrBI CCG'CTC
Cuts at: 1394 1823 2091 2256 2298 2491 2655 2686 2800
Size: 429 268 165 42 193 164 31 114
Cuts at: 2800 5153 5173 5782 6375 1394
Size: 2353 20 609 593 3507
Fragments arranged by size:
3507 2353 609 593 429 268 193 165
164 114 42 31 20
BsrDI GCAATG_nn'
Cuts at: 65 1248 5185 6983 7157 65
Size: 1183 3937 1798 174 1396
Fragments arranged by size:
3937 1798 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 2352 2528 5112 5639 7132 7319
Size: 110 252 290 176 2584 527 1493 187
Cuts at: 7319 1700
Size: 2869
Fragments arranged by size:
2869 2584 1493 527 290 252 187 176
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8488
BssHII G'CGCG_C
Cuts at: 1925 2506 1925
Size: 581 7907
BssSI C'ACGA_G
Cuts at: 2756 6548 7560 2756
Size: 3792 1012 3684
Fragments arranged by size:
3792 3684 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8488
BstBI TT'CG_AA
Cuts at: 7292 7292
Size: 8488
BstEII G'GTnAC_C
Cuts at: 1184 2185 4888 5662 1184
Size: 1001 2703 774 4010
Fragments arranged by size:
4010 2703 1001 774
BstXI CCAn_nnnn'nTGG
Cuts at: 2740 3178 3652 3787 4023 4058 4534 5146 5629
Size: 438 474 135 236 35 476 612 483
Cuts at: 5629 2740
Size: 5599
Fragments arranged by size:
5599 612 483 476 474 438 236 135
35
BstYI r'GATC_y
Cuts at: 1972 3546 5818 6185 6570 6587 8039 8125 8137
Size: 1574 2272 367 385 17 1452 86 12
Cuts at: 8137 8483 1972
Size: 346 1977
Fragments arranged by size:
2272 1977 1574 1452 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4140 4140
Size: 8488
BtgI C'CryG_G
Cuts at: 513 1065 2155 2925 3327 5553 513
Size: 552 1090 770 402 2226 3448
Fragments arranged by size:
3448 2226 1090 770 552 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2905 3079 4692 5218 5299 487
Size: 683 56 1679 174 1613 526 81 3676
Fragments arranged by size:
3676 1679 1613 683 526 174 81 56
BtsI GCAGTG_nn'
Cuts at: 3060 3459 3607 3715 4460 5034 6004 6798 6818
Size: 399 148 108 745 574 970 794 20
Cuts at: 6818 3060
Size: 4730
Fragments arranged by size:
4730 970 794 745 574 399 148 108
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4935 4970 529
Size: 35 4371 35 4047
Fragments arranged by size:
4371 4047 35 35
DraI TTT'AAA
Cuts at: 5792 5792
Size: 8488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4886 7495 809
Size: 507 3570 2609 1802
Fragments arranged by size:
3570 2609 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2271 2370
Size: 54 1425 50 275 18 381 60 99
Cuts at: 2370 2511 2742 2817 2829 2934 3090 3516 4656
Size: 141 231 75 12 105 156 426 1140
Cuts at: 4656 4815 5148 5457 5559 5619 5935 6824 8453
Size: 159 333 309 102 60 316 889 1629
Cuts at: 8453 8
Size: 43
Fragments arranged by size:
1629 1425 1140 889 426 381 333 316
309 275 231 159 156 141 105 102
99 75 60 60 54 50 43 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 2511 2829 4656 5935 1537
Size: 275 699 318 1827 1279 4090
Fragments arranged by size:
4090 1827 1279 699 318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3500 3951 4328 4454 4871 6418 1353
Size: 2147 451 377 126 417 1547 3423
Fragments arranged by size:
3423 2147 1547 451 417 377 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2296 2591 2699 3067 4664 7075
Size: 1064 138 707 295 108 368 1597 2411
Cuts at: 7075 7449 7595 387
Size: 374 146 1280
Fragments arranged by size:
2411 1597 1280 1064 707 374 368 295
146 138 108
EcoICRI GAG'CTC
Cuts at: 3493 4825 5188 3493
Size: 1332 363 6793
Fragments arranged by size:
6793 1332 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 2504 2543 2702 2786 3152 3606
Size: 393 640 232 39 159 84 366 454
Cuts at: 3606 4219 4459 4561 5285 5381 5729 6551 7148
Size: 613 240 102 724 96 348 822 597
Cuts at: 7148 7935 8203 1239
Size: 787 268 1524
Fragments arranged by size:
1524 822 787 724 640 613 597 454
393 366 348 268 240 232 159 102
96 84 39
EcoNI CCTnn'n_nnAGG
Cuts at: 5138 5138
Size: 8488
EcoO109I rG'GnC_Cy
Cuts at: 2078 2597 4582 4923 5774 5775 2078
Size: 519 1985 341 851 1 4791
Fragments arranged by size:
4791 1985 851 519 341 1
EcoRI G'AATT_C
Cuts at: 3696 5797 6175 3696
Size: 2101 378 6009
Fragments arranged by size:
6009 2101 378
FspI TGC'GCA
Cuts at: 3041 3104 6994 3041
Size: 63 3890 4535
Fragments arranged by size:
4535 3890 63
FspAI rTGC'GCAy
Cuts at: 3041 3041
Size: 8488
HaeII r_GCGC'y
Cuts at: 1678 2063 2069 2209 2396 2535 2625 2701 2979
Size: 385 6 140 187 139 90 76 278
Cuts at: 2979 4039 7635 1678
Size: 1060 3596 2531
Fragments arranged by size:
3596 2531 1060 385 278 187 140 139
90 76 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2570 2602 3231 3263 3743
Size: 32 135 32 998 32 629 32 480
Cuts at: 3743 3775 7129 7161 7203 7235 1373
Size: 32 3354 32 42 32 2626
Fragments arranged by size:
3354 2626 998 629 480 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4558 4633 4663 5832 6078 669
Size: 988 281 2620 75 30 1169 246 3079
Fragments arranged by size:
3079 2620 1169 988 281 246 75 30
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8488
HpaI GTT'AAC
Cuts at: 6078 6078
Size: 8488
KasI G'GCGC_C
Cuts at: 2059 2065 2392 2531 2621 2059
Size: 6 327 139 90 7926
Fragments arranged by size:
7926 327 139 90 6
KpnI G_GTAC'C
Cuts at: 5813 5813
Size: 8488
MfeI C'AATT_G
Cuts at: 6087 6087
Size: 8488
MluI A'CGCG_T
Cuts at: 4635 8364 4635
Size: 3729 4759
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2652 7602 7786 1305
Size: 1347 4950 184 2007
Fragments arranged by size:
4950 2007 1347 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 3092 4817 5621 10
Size: 54 1425 343 381 879 1725 804 2877
Fragments arranged by size:
2877 1725 1425 879 804 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 2744 2858 2924 3332 3612 3632
Size: 648 1572 6 114 66 408 280 20
Cuts at: 3632 3689 3720 4056 4349 4367 4535 4832 5390
Size: 57 31 336 293 18 168 297 558
Cuts at: 5390 5672 6446 6805 6964 518
Size: 282 774 359 159 2042
Fragments arranged by size:
2042 1572 774 648 558 408 359 336
297 293 282 280 168 159 114 66
57 31 20 18 6
MspA1I CmG'CkG
Cuts at: 1606 2157 2220 2251 2359 2376 2806 3063 3271
Size: 551 63 31 108 17 430 257 208
Cuts at: 3271 3705 4788 5359 5707 5904 6581 7729 7974
Size: 434 1083 571 348 197 677 1148 245
Cuts at: 7974 1606
Size: 2120
Fragments arranged by size:
2120 1148 1083 677 571 551 434 430
348 257 245 208 197 108 63 31
17
NaeI GCC'GGC
Cuts at: 2064 2354 2530 2064
Size: 290 176 8022
Fragments arranged by size:
8022 290 176
NarI GG'CG_CC
Cuts at: 2060 2066 2393 2532 2622 2060
Size: 6 327 139 90 7926
Fragments arranged by size:
7926 327 139 90 6
NcoI C'CATG_G
Cuts at: 513 1065 3327 513
Size: 552 2262 5674
Fragments arranged by size:
5674 2262 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8488
NgoMIV G'CCGG_C
Cuts at: 2062 2352 2528 2062
Size: 290 176 8022
Fragments arranged by size:
8022 290 176
NheI G'CTAG_C
Cuts at: 1052 3775 1052
Size: 2723 5765
NotI GC'GGCC_GC
Cuts at: 2829 5935 2829
Size: 3106 5382
NspI r_CATG'y
Cuts at: 1132 1229 3046 3688 4564 4946 5677 5846 7391
Size: 97 1817 642 876 382 731 169 1545
Cuts at: 7391 1132
Size: 2229
Fragments arranged by size:
2229 1817 1545 876 731 642 382 169
97
PasI CC'CwG_GG
Cuts at: 1348 2617 1348
Size: 1269 7219
PciI A'CATG_T
Cuts at: 4560 5673 7387 4560
Size: 1113 1714 5661
Fragments arranged by size:
5661 1714 1113
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2220 2947 3875 5026 5686 1108
Size: 1112 727 928 1151 660 3910
Fragments arranged by size:
3910 1151 1112 928 727 660
PfoI T'CCnGG_A
Cuts at: 3228 3657 4475 3228
Size: 429 818 7241
Fragments arranged by size:
7241 818 429
PmeI GTTT'AAAC
Cuts at: 5792 5792
Size: 8488
PmlI CAC'GTG
Cuts at: 4569 4645 4569
Size: 76 8412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5333 5365 6557 6589 8070 8102 5333
Size: 32 1192 32 1481 32 5719
Fragments arranged by size:
5719 1481 1192 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4582 4923 4582
Size: 341 8147
PshAI GACnn'nnGTC
Cuts at: 1613 2337 1613
Size: 724 7764
PsiI TTA'TAA
Cuts at: 6058 6058
Size: 8488
PspOMI G'GGCC_C
Cuts at: 2597 5774 2597
Size: 3177 5311
PstI C_TGCA'G
Cuts at: 830 2646 4467 4782 5067 5103 5840 830
Size: 1816 1821 315 285 36 737 3478
Fragments arranged by size:
3478 1821 1816 737 315 285 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8488
PvuII CAG'CTG
Cuts at: 2806 4788 5359 5707 2806
Size: 1982 571 348 5587
Fragments arranged by size:
5587 1982 571 348
SacI G_AGCT'C
Cuts at: 3495 4827 5190 3495
Size: 1332 363 6793
Fragments arranged by size:
6793 1332 363
SacII CC_GC'GG
Cuts at: 2158 2158
Size: 8488
SalI G'TCGA_C
Cuts at: 1936 5830 1936
Size: 3894 4594
SapI GCTCTTCn'nnn_
Cuts at: 3500 4454 4871 3500
Size: 954 417 7117
Fragments arranged by size:
7117 954 417
SbfI CC_TGCA'GG
Cuts at: 5840 5840
Size: 8488
ScaI AGT'ACT
Cuts at: 4262 4730 6736 8448 4262
Size: 468 2006 1712 4302
Fragments arranged by size:
4302 2006 1712 468
SexAI A'CCwGG_T
Cuts at: 4065 5679 4065
Size: 1614 6874
SfcI C'TryA_G
Cuts at: 826 1045 2642 4463 4666 4738 4778 5063 5099
Size: 219 1597 1821 203 72 40 285 36
Cuts at: 5099 5836 6971 7652 7843 826
Size: 737 1135 681 191 1471
Fragments arranged by size:
1821 1597 1471 1135 737 681 285 219
203 191 72 40 36
SfoI GGC'GCC
Cuts at: 2061 2067 2394 2533 2623 2061
Size: 6 327 139 90 7926
Fragments arranged by size:
7926 327 139 90 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8488
SgrAI Cr'CCGG_yG
Cuts at: 1700 2062 5639 1700
Size: 362 3577 4549
Fragments arranged by size:
4549 3577 362
SmaI CCC'GGG
Cuts at: 1872 2034 2072 2143 2176 2390 5815 1872
Size: 162 38 71 33 214 3425 4545
Fragments arranged by size:
4545 3425 214 162 71 38 33
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3899 6592 7493 7755 820
Size: 197 926 1956 2693 901 262 1553
Fragments arranged by size:
2693 1956 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3539 6279 493
Size: 3046 2740 2702
Fragments arranged by size:
3046 2740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8488
SphI G_CATG'C
Cuts at: 1132 3046 4946 5846 1132
Size: 1914 1900 900 3774
Fragments arranged by size:
3774 1914 1900 900
SspI AAT'ATT
Cuts at: 5 52 6412 5
Size: 47 6360 2081
Fragments arranged by size:
6360 2081 47
StuI AGG'CCT
Cuts at: 1517 5105 1517
Size: 3588 4900
StyI C'CwwG_G
Cuts at: 513 1065 3327 3550 3727 3825 4074 4107 4407
Size: 552 2262 223 177 98 249 33 300
Cuts at: 4407 4950 5139 5927 513
Size: 543 189 788 3074
Fragments arranged by size:
3074 2262 788 552 543 300 249 223
189 177 98 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3170 3206 3956 4046 4191 4296 6515
Size: 1255 1590 36 750 90 145 105 2219
Cuts at: 6515 6532 6685 6870 7315 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2219 1590 1498 1255 750 445 185 153
145 105 90 36 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3712 4260 4728
Size: 274 80 33 51 1443 1735 548 468
Cuts at: 4728 5493 6734 8446 96
Size: 765 1241 1712 138
Fragments arranged by size:
1735 1712 1443 1241 765 548 468 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3181 3213 8448 8480 3181
Size: 32 5235 32 3189
Fragments arranged by size:
5235 3189 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8488
XbaI T'CTAG_A
Cuts at: 5824 5824
Size: 8488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3791 4585 5476 3791
Size: 794 891 6803
Fragments arranged by size:
6803 891 794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8488
XmaI C'CCGG_G
Cuts at: 1870 2032 2070 2141 2174 2388 5813 1870
Size: 162 38 71 33 214 3425 4545
Fragments arranged by size:
4545 3425 214 162 71 38 33
XmnI GAAnn'nnTTC
Cuts at: 6617 6617
Size: 8488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3754 6296 276
Size: 53 83 186 936 2220 2542 2468
Fragments arranged by size:
2542 2468 2220 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BstZ17I DraIII EcoRV FalI
FseI NruI NsiI PacI PspXI PsrI RsrII SanDI
SfiI SrfI SwaI