Restriction map for
pFN21AE0094
(Circular) (Six-base) MAPSORT of: pFN21AE0094.seq Check: 4607 from: 1 to: 6310
pFN21AE0094 6310 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4120 278
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2760 3653 1937
Size: 823 893 4594
Fragments arranged by size:
4594 893 823
Acc65I G'GTAC_C
Cuts at: 2232 3631 2232
Size: 1399 4911
AclI AA'CG_TT
Cuts at: 1563 4437 4810 1563
Size: 2874 373 3063
Fragments arranged by size:
3063 2874 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2353 2966 3092 3146 4373 5757 6025 1632
Size: 721 613 126 54 1227 1384 268 1917
Fragments arranged by size:
1917 1384 1227 721 613 268 126 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6113
AflIII A'CryG_T
Cuts at: 1203 5209 6186 1203
Size: 4006 977 1327
Fragments arranged by size:
4006 1327 977
AgeI A'CCGG_T
Cuts at: 3373 5141 3373
Size: 1768 4542
AhdI GACnn_n'nnGTC
Cuts at: 5039 5039
Size: 6310
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2120 2579 1184
Size: 936 459 4915
Fragments arranged by size:
4915 936 459
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2235 2267 2235
Size: 32 6278
AlwNI CAG_nnn'CTG
Cuts at: 2689 2857 3124 4053 5625 2689
Size: 168 267 929 1572 3374
Fragments arranged by size:
3374 1572 929 267 168
ApaI G_GGCC'C
Cuts at: 2710 2710
Size: 6310
ApaLI G'TGCA_C
Cuts at: 4367 5523 4367
Size: 1156 5154
ApoI r'AATT_y
Cuts at: 1493 3619 3845 3997 6106 1493
Size: 2126 226 152 2109 1697
Fragments arranged by size:
2126 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4864 160
Size: 872 3832 1606
Fragments arranged by size:
3832 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 3635 1870
Size: 73 105 1587 4545
Fragments arranged by size:
4545 1587 105 73
BamHI G'GATC_C
Cuts at: 2353 2472 3640 4007 2353
Size: 119 1168 367 4656
Fragments arranged by size:
4656 1168 367 119
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2232 3251 3631 5086 618
Size: 325 217 627 445 1019 380 1455 1842
Fragments arranged by size:
1842 1455 1019 627 445 380 325 217
BanII G_rGCy'C
Cuts at: 1391 2079 2280 2710 3629 1391
Size: 688 201 430 919 4072
Fragments arranged by size:
4072 919 688 430 201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2222 928
Size: 971 323 5016
Fragments arranged by size:
5016 971 323
BbvCI CC'TCA_GC
Cuts at: 2823 2823
Size: 6310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3488 3522 3551 3585 4501 4535 1903
Size: 34 1551 34 29 34 916 34 3678
Fragments arranged by size:
3678 1551 916 34 34 34 34 29
BciVI GTATCCnnnnn_n'
Cuts at: 4202 5412 4202
Size: 1210 5100
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6310
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2912 4921 136
Size: 107 122 71 631 605 1240 2009 1525
Fragments arranged by size:
2009 1525 1240 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3015 3045 3354 3453 6305 3015
Size: 30 309 99 2852 3020
Fragments arranged by size:
3020 2852 309 99 30
BlpI GC'TnA_GC
Cuts at: 3727 3727
Size: 6310
Bme1580I G_kGCm'C
Cuts at: 1790 2422 2710 3254 3549 4371 5527 1790
Size: 632 288 544 295 822 1156 2573
Fragments arranged by size:
2573 1156 822 632 544 295 288
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6310
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2220 4041 4999
Size: 416 53 83 399 24 803 1821 958
Cuts at: 4999 442
Size: 1753
Fragments arranged by size:
1821 1753 958 803 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6310
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2977 2158
Size: 819 5491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2462 3161 3315 3560 4970 1239
Size: 935 288 699 154 245 1410 2579
Fragments arranged by size:
2579 1410 935 699 288 245 154
Bpu10I CC'TnA_GC
Cuts at: 2274 2823 2274
Size: 549 5761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2549 2609 4435 5300 5598 2063
Size: 486 60 1826 865 298 2775
Fragments arranged by size:
2775 1826 865 486 298 60
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4973 882
Size: 617 3474 2219
Fragments arranged by size:
3474 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4101 493
Size: 711 2897 2702
Fragments arranged by size:
2897 2702 711
BsaBI GATnn'nnATC
Cuts at: 6304 6304
Size: 6310
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4117 4499 5134 275
Size: 53 83 186 936 2584 382 635 1451
Fragments arranged by size:
2584 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2805 3373 4743 5141 5415 5562 5973 2805
Size: 568 1370 398 274 147 411 3142
Fragments arranged by size:
3142 1370 568 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2753 2783 1178
Size: 30 165 30 1350 30 4705
Fragments arranged by size:
4705 1350 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2780 3218 1186
Size: 1594 438 4278
Fragments arranged by size:
4278 1594 438
BseYI C'CCAG_C
Cuts at: 2017 2421 3177 3445 5513 6009 2017
Size: 404 756 268 2068 496 2318
Fragments arranged by size:
2318 2068 756 496 404 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3242 1163
Size: 573 1506 4231
Fragments arranged by size:
4231 1506 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2032 3760 4521 5549 1540
Size: 275 186 31 1728 761 1028 2301
Fragments arranged by size:
2301 1728 1028 761 275 186 31
BsiHKAI G_wGCw'C
Cuts at: 3042 4371 4456 5527 3042
Size: 1329 85 1071 3825
Fragments arranged by size:
3825 1329 1071 85
BsmI GAATG_Cn'
Cuts at: 3821 3914 3821
Size: 93 6217
BsmBI CGTCTCn'nnnn_
Cuts at: 2672 2672
Size: 6310
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2280 2422 2710 3042 3254 3549
Size: 399 289 201 142 288 332 212 295
Cuts at: 3549 3629 4371 4456 5527 1391
Size: 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 332 295 289 288
212 201 142 85 80
BspEI T'CCGG_A
Cuts at: 5973 5973
Size: 6310
BspHI T'CATG_A
Cuts at: 4199 5929 4199
Size: 1730 4580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 3665 844
Size: 1400 1421 3489
Fragments arranged by size:
3489 1421 1400
BsrBI CCG'CTC
Cuts at: 1394 1823 2545 3034 4197 1394
Size: 429 722 489 1163 3507
Fragments arranged by size:
3507 1163 722 489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2295 3171 4805 4979 65
Size: 1183 1047 876 1634 174 1396
Fragments arranged by size:
1634 1396 1183 1047 876 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3373 4954 5141 1700
Size: 110 1563 1581 187 2869
Fragments arranged by size:
2869 1581 1563 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6310
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6310
BssSI C'ACGA_G
Cuts at: 2132 2633 3548 4370 5382 2132
Size: 501 915 822 1012 3060
Fragments arranged by size:
3060 1012 915 822 501
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2692 3553 794
Size: 1898 861 3551
Fragments arranged by size:
3551 1898 861
BstBI TT'CG_AA
Cuts at: 5114 5114
Size: 6310
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6310
BstXI CCAn_nnnn'nTGG
Cuts at: 3226 3226
Size: 6310
BstYI r'GATC_y
Cuts at: 1972 2353 2472 3015 3045 3354 3453 3640 4007
Size: 381 119 543 30 309 99 187 367
Cuts at: 4007 4392 4409 5861 5947 5959 6305 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 543 385 381 367 346 309
187 119 99 86 30 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2181 513
Size: 552 1116 4642
Fragments arranged by size:
4642 1116 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5571
Fragments arranged by size:
5571 683 56
BtsI GCAGTG_nn'
Cuts at: 3826 4620 4640 3826
Size: 794 20 5496
Fragments arranged by size:
5496 794 20
ClaI AT'CG_AT
Cuts at: 1554 3306 1554
Size: 1752 4558
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6275
DraI TTT'AAA
Cuts at: 3614 3614
Size: 6310
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5317 809
Size: 507 4001 1802
Fragments arranged by size:
4001 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2913 3009 3022
Size: 54 1425 50 275 18 1083 96 13
Cuts at: 3022 3327 3465 3513 3757 4646 6275 8
Size: 305 138 48 244 889 1629 43
Fragments arranged by size:
1629 1425 1083 889 305 275 244 138
96 54 50 48 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3757 1537
Size: 275 1945 4090
Fragments arranged by size:
4090 1945 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2573 3068 3122 4240 1353
Size: 756 464 495 54 1118 3423
Fragments arranged by size:
3423 1118 756 495 464 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3252 4897 5271 5417 387
Size: 1064 138 1663 1645 374 146 1280
Fragments arranged by size:
1663 1645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2353 2462 2966 3092 3146 3161
Size: 393 542 179 109 504 126 54 15
Cuts at: 3161 3315 3560 4373 4970 5757 6025 1239
Size: 154 245 813 597 787 268 1524
Fragments arranged by size:
1524 813 787 597 542 504 393 268
245 179 154 126 109 54 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2227 2227
Size: 6310
EcoO109I rG'GnC_Cy
Cuts at: 2044 2706 2707 2833 2044
Size: 662 1 126 5521
Fragments arranged by size:
5521 662 126 1
EcoRI G'AATT_C
Cuts at: 3619 3997 3619
Size: 378 5932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 2383 2239
Size: 144 6166
FspI TGC'GCA
Cuts at: 2879 4816 2879
Size: 1937 4373
HaeII r_GCGC'y
Cuts at: 1678 3399 5457 1678
Size: 1721 2058 2531
Fragments arranged by size:
2531 2058 1721
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2588 2620 3288 3320 4951
Size: 32 135 32 1016 32 668 32 1631
Cuts at: 4951 4983 5025 5057 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1631 1016 668 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2551 3001 3654 3900 669
Size: 988 281 613 450 653 246 3079
Fragments arranged by size:
3079 988 653 613 450 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6310
HpaI GTT'AAC
Cuts at: 3900 3900
Size: 6310
KpnI G_GTAC'C
Cuts at: 2236 3635 2236
Size: 1399 4911
MfeI C'AATT_G
Cuts at: 3909 3909
Size: 6310
MluI A'CGCG_T
Cuts at: 6186 6186
Size: 6310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5424 5608 1305
Size: 4119 184 2007
Fragments arranged by size:
4119 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2915 3329 3515 10
Size: 54 1425 343 1083 414 186 2805
Fragments arranged by size:
2805 1425 1083 414 343 186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 3224 3279 4268 4627 4786 518
Size: 648 1134 924 55 989 359 159 2042
Fragments arranged by size:
2042 1134 989 924 648 359 159 55
MspA1I CmG'CkG
Cuts at: 1606 2013 2522 2689 3091 3124 3214 3726 4403
Size: 407 509 167 402 33 90 512 677
Cuts at: 4403 5551 5796 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 512 509 407 402 245
167 90 33
NcoI C'CATG_G
Cuts at: 513 1065 2181 513
Size: 552 1116 4642
Fragments arranged by size:
4642 1116 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6310
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6310
NotI GC'GGCC_GC
Cuts at: 3757 3757
Size: 6310
NspI r_CATG'y
Cuts at: 1132 1229 3160 3490 3668 5213 1132
Size: 97 1931 330 178 1545 2229
Fragments arranged by size:
2229 1931 1545 330 178 97
PasI CC'CwG_GG
Cuts at: 1348 2333 1348
Size: 985 5325
PciI A'CATG_T
Cuts at: 5209 5209
Size: 6310
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2584 3184 1108
Size: 1476 600 4234
Fragments arranged by size:
4234 1476 600
PmeI GTTT'AAAC
Cuts at: 3614 3614
Size: 6310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4379 4411 5892 5924 4379
Size: 32 1481 32 4765
Fragments arranged by size:
4765 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2044 2833 2044
Size: 789 5521
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6310
PsiI TTA'TAA
Cuts at: 3880 3880
Size: 6310
PspOMI G'GGCC_C
Cuts at: 2706 2706
Size: 6310
PstI C_TGCA'G
Cuts at: 830 3096 3662 830
Size: 2266 566 3478
Fragments arranged by size:
3478 2266 566
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6310
PvuII CAG'CTG
Cuts at: 2013 2522 2689 3091 2013
Size: 509 167 402 5232
Fragments arranged by size:
5232 509 402 167
SalI G'TCGA_C
Cuts at: 1936 3652 1936
Size: 1716 4594
SapI GCTCTTCn'nnn_
Cuts at: 2109 2573 3122 2109
Size: 464 549 5297
Fragments arranged by size:
5297 549 464
SbfI CC_TGCA'GG
Cuts at: 3662 3662
Size: 6310
ScaI AGT'ACT
Cuts at: 2204 4558 6270 2204
Size: 2354 1712 2244
Fragments arranged by size:
2354 2244 1712
SfcI C'TryA_G
Cuts at: 826 1045 3092 3658 4793 5474 5665 826
Size: 219 2047 566 1135 681 191 1471
Fragments arranged by size:
2047 1471 1135 681 566 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2912 2912
Size: 6310
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
SgrAI Cr'CCGG_yG
Cuts at: 1700 3373 1700
Size: 1673 4637
SmaI CCC'GGG
Cuts at: 1872 3637 1872
Size: 1765 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2528 2624 4414 5315 5577
Size: 197 926 135 450 96 1790 901 262
Cuts at: 5577 820
Size: 1553
Fragments arranged by size:
1790 1553 926 901 450 262 197 135
96
SnaBI TAC'GTA
Cuts at: 493 4101 493
Size: 3608 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6310
SphI G_CATG'C
Cuts at: 1132 3490 3668 1132
Size: 2358 178 3774
Fragments arranged by size:
3774 2358 178
SspI AAT'ATT
Cuts at: 5 52 4234 5
Size: 47 4182 2081
Fragments arranged by size:
4182 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6310
StyI C'CwwG_G
Cuts at: 513 1065 2109 2181 2721 2916 3749 513
Size: 552 1044 72 540 195 833 3074
Fragments arranged by size:
3074 1044 833 552 540 195 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3310 4337 4354 4507 4692 5137 325
Size: 1255 1730 1027 17 153 185 445 1498
Fragments arranged by size:
1730 1498 1255 1027 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 4556 6268
Size: 274 80 33 51 1443 225 2354 1712
Cuts at: 6268 96
Size: 138
Fragments arranged by size:
2354 1712 1443 274 225 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2457 2489 6270 6302 2457
Size: 32 3781 32 2465
Fragments arranged by size:
3781 2465 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6310
XbaI T'CTAG_A
Cuts at: 3646 3646
Size: 6310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2188 2207 3050 3412 3442 2188
Size: 19 843 362 30 5056
Fragments arranged by size:
5056 843 362 30 19
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6310
XmaI C'CCGG_G
Cuts at: 1870 3635 1870
Size: 1765 4545
XmnI GAAnn'nnTTC
Cuts at: 2366 2408 4439 2366
Size: 42 2031 4237
Fragments arranged by size:
4237 2031 42
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4118 276
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI
SrfI SwaI