Restriction map for
pFN21AE0092
(Circular) (Six-base) MAPSORT of: pFN21AE0092.seq Check: 6510 from: 1 to: 10420
pFN21AE0092 10420 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2065 7174 2065
Size: 5109 5311
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5374 8230 278
Size: 53 83 186 936 3838 2856 2468
Fragments arranged by size:
3838 2856 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3964 7763 1937
Size: 2027 3799 4594
Fragments arranged by size:
4594 3799 2027
Acc65I G'GTAC_C
Cuts at: 2462 6804 7491 7741 2462
Size: 4342 687 250 5141
Fragments arranged by size:
5141 4342 687 250
AclI AA'CG_TT
Cuts at: 1563 8547 8920 1563
Size: 6984 373 3063
Fragments arranged by size:
6984 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 2661 2765 3040 3192 3453 3817 3823
Size: 555 474 104 275 152 261 364 6
Cuts at: 3823 4328 4736 5106 5651 5699 5705 5711 7656
Size: 505 408 370 545 48 6 6 1945
Cuts at: 7656 8483 9867 10135 1632
Size: 827 1384 268 1917
Fragments arranged by size:
1945 1917 1384 827 555 545 505 474
408 370 364 275 268 261 152 104
48 6 6 6
AflII C'TTAA_G
Cuts at: 820 1017 5618 820
Size: 197 4601 5622
Fragments arranged by size:
5622 4601 197
AflIII A'CryG_T
Cuts at: 1203 6620 7037 9319 10296 1203
Size: 5417 417 2282 977 1327
Fragments arranged by size:
5417 2282 1327 977 417
AgeI A'CCGG_T
Cuts at: 9251 9251
Size: 10420
AhdI GACnn_n'nnGTC
Cuts at: 4813 9149 4813
Size: 4336 6084
AleI CACnn'nnGTG
Cuts at: 1166 7392 1166
Size: 6226 4194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4935 5330 6504 1184
Size: 3751 395 1174 5100
Fragments arranged by size:
5100 3751 1174 395
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5116 5148 5116
Size: 32 10388
AlwNI CAG_nnn'CTG
Cuts at: 3242 3512 4507 5933 6032 8163 9735 3242
Size: 270 995 1426 99 2131 1572 3927
Fragments arranged by size:
3927 2131 1572 1426 995 270 99
ApaI G_GGCC'C
Cuts at: 5569 5569
Size: 10420
ApaLI G'TGCA_C
Cuts at: 8477 9633 8477
Size: 1156 9264
ApoI r'AATT_y
Cuts at: 1493 2527 7196 7729 7955 8107 10216 1493
Size: 1034 4669 533 226 152 2109 1697
Fragments arranged by size:
4669 2109 1697 1034 533 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8974 160
Size: 872 7942 1606
Fragments arranged by size:
7942 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10420
AvaI C'yCGr_G
Cuts at: 1870 1943 2353 5562 5568 5594 6375 6947 7745
Size: 73 410 3209 6 26 781 572 798
Cuts at: 7745 1870
Size: 4545
Fragments arranged by size:
4545 3209 798 781 572 410 73 26
6
AvrII C'CTAG_G
Cuts at: 3124 5609 3124
Size: 2485 7935
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6788 6821 6788
Size: 33 10387
BamHI G'GATC_C
Cuts at: 7750 8117 7750
Size: 367 10053
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2462 2920 6724 6804 6875
Size: 325 217 627 675 458 3804 80 71
Cuts at: 6875 6887 6936 6954 7491 7741 9196 618
Size: 12 49 18 537 250 1455 1842
Fragments arranged by size:
3804 1842 1455 675 627 537 458 325
250 217 80 71 49 18 12
BanII G_rGCy'C
Cuts at: 1391 2907 4950 5569 6477 6819 7350 7739 1391
Size: 1516 2043 619 908 342 531 389 4072
Fragments arranged by size:
4072 2043 1516 908 619 531 389 342
BbeI G_GCGC'C
Cuts at: 6940 6940
Size: 10420
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2877 2898 3391 3656 6536 6654 928
Size: 971 978 21 493 265 2880 118 4694
Fragments arranged by size:
4694 2880 978 971 493 265 118 21
BbvCI CC'TCA_GC
Cuts at: 3424 5709 5795 6057 6454 6729 3424
Size: 2285 86 262 397 275 7115
Fragments arranged by size:
7115 2285 397 275 262 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8611 8645 1903
Size: 34 6674 34 3678
Fragments arranged by size:
6674 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2651 6321 6392 8312 9522 2651
Size: 3670 71 1920 1210 3549
Fragments arranged by size:
3670 3549 1920 1210 71
BclI T'GATC_A
Cuts at: 1548 2860 3244 3783 1548
Size: 1312 384 539 8185
Fragments arranged by size:
8185 1312 539 384
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6823 9031 136
Size: 107 122 71 631 605 5151 2208 1525
Fragments arranged by size:
5151 2208 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4767 10415 4767
Size: 5648 4772
BlpI GC'TnA_GC
Cuts at: 5679 5822 6860 7179 7837 5679
Size: 143 1038 319 658 8262
Fragments arranged by size:
8262 1038 658 319 143
Bme1580I G_kGCm'C
Cuts at: 1790 5569 6880 6890 7656 8481 9637 1790
Size: 3779 1311 10 766 825 1156 2573
Fragments arranged by size:
3779 2573 1311 1156 825 766 10
BmgBI CAC'GTC
Cuts at: 1318 3577 1318
Size: 2259 8161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2341 4539 5085
Size: 416 53 83 399 24 924 2198 546
Cuts at: 5085 5389 5783 6231 8151 9109 442
Size: 304 394 448 1920 958 1753
Fragments arranged by size:
2198 1920 1753 958 924 546 448 416
399 394 304 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10420
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 5847 6328 6688 7648 3617
Size: 2230 481 360 960 6389
Fragments arranged by size:
6389 2230 960 481 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2218 2921 3315 3339 3375 3399 3612 4112
Size: 979 703 394 24 36 24 213 500
Cuts at: 4112 5012 5768 6099 6198 6596 6866 7016 9080
Size: 900 756 331 99 398 270 150 2064
Cuts at: 9080 1239
Size: 2579
Fragments arranged by size:
2579 2064 979 900 756 703 500 398
394 331 270 213 150 99 36 24
24
Bpu10I CC'TnA_GC
Cuts at: 3424 3794 5709 5795 6057 6313 6454 6729 7547
Size: 370 1915 86 262 256 141 275 818
Cuts at: 7547 3424
Size: 6297
Fragments arranged by size:
6297 1915 818 370 275 262 256 141
86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2309 3017 5946 6344 8545 9410 9708 2309
Size: 708 2929 398 2201 865 298 3021
Fragments arranged by size:
3021 2929 2201 865 708 398 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2121 2806 3415 3725 4220 5144 5350
Size: 617 622 685 609 310 495 924 206
Cuts at: 5350 6165 6223 9083 882
Size: 815 58 2860 2219
Fragments arranged by size:
2860 2219 924 815 685 622 617 609
495 310 206 58
BsaAI yAC'GTr
Cuts at: 493 1204 8211 493
Size: 711 7007 2702
Fragments arranged by size:
7007 2702 711
BsaBI GATnn'nnATC
Cuts at: 10414 10414
Size: 10420
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5371 6937 7285 8227
Size: 53 83 186 936 3838 1566 348 942
Cuts at: 8227 8609 9244 275
Size: 382 635 1451
Fragments arranged by size:
3838 1566 1451 942 936 635 382 348
186 83 53
BsaWI w'CCGG_w
Cuts at: 5043 8853 9251 9525 9672 10083 5043
Size: 3810 398 274 147 411 5380
Fragments arranged by size:
5380 3810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3722 3752 3914 3944 4769
Size: 30 165 30 2319 30 162 30 825
Cuts at: 4769 4799 4985 5015 5116 5146 7418 7448 1178
Size: 30 186 30 101 30 2272 30 4150
Fragments arranged by size:
4150 2319 2272 825 186 165 162 101
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2221 3646 3935 4130 4376 5162 5195 5456
Size: 1035 1425 289 195 246 786 33 261
Cuts at: 5456 5936 6061 6584 7586 7703 1186
Size: 480 125 523 1002 117 3903
Fragments arranged by size:
3903 1425 1035 1002 786 523 480 289
261 246 195 125 117 33
BseYI C'CCAG_C
Cuts at: 2133 2258 2435 3231 3307 3436 3505 3532 4606
Size: 125 177 796 76 129 69 27 1074
Cuts at: 4606 4884 4899 4908 5661 5697 5703 5899 6143
Size: 278 15 9 753 36 6 196 244
Cuts at: 6143 6205 6276 6288 7315 7649 7683 9623 10119
Size: 62 71 12 1027 334 34 1940 496
Cuts at: 10119 2133
Size: 2434
Fragments arranged by size:
2434 1940 1074 1027 796 753 496 334
278 244 196 177 129 125 76 71
69 62 36 34 27 15 12 9
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2037 2997 3926 4707 6140 1163
Size: 573 301 960 929 781 1433 5443
Fragments arranged by size:
5443 1433 960 929 781 573 301
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7870 8631 9659 1540
Size: 275 186 5869 761 1028 2301
Fragments arranged by size:
5869 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3831 4950 6314 6669 8481 8566 9637 3831
Size: 1119 1364 355 1812 85 1071 4614
Fragments arranged by size:
4614 1812 1364 1119 1071 355 85
BsmI GAATG_Cn'
Cuts at: 2601 5117 6924 7931 8024 2601
Size: 2516 1807 1007 93 4997
Fragments arranged by size:
4997 2516 1807 1007 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3628 3628
Size: 10420
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2907 3831 4950 5569 6314 6477 6669
Size: 399 1117 924 1119 619 745 163 192
Cuts at: 6669 6819 6880 6890 7350 7656 7739 8481 8566
Size: 150 61 10 460 306 83 742 85
Cuts at: 8566 9637 1391
Size: 1071 2174
Fragments arranged by size:
2174 1119 1117 1071 924 745 742 619
460 399 306 192 163 150 85 83
61 10
BspEI T'CCGG_A
Cuts at: 5043 10083 5043
Size: 5040 5380
BspHI T'CATG_A
Cuts at: 3627 6832 8309 10039 3627
Size: 3205 1477 1730 4008
Fragments arranged by size:
4008 3205 1730 1477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2065 4707 6496 6816 7174 7775 844
Size: 1221 2642 1789 320 358 601 3489
Fragments arranged by size:
3489 2642 1789 1221 601 358 320
BsrBI CCG'CTC
Cuts at: 1394 1823 6952 7540 8307 1394
Size: 429 5129 588 767 3507
Fragments arranged by size:
5129 3507 767 588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4538 6007 7005 8915 9089 65
Size: 1183 3290 1469 998 1910 174 1396
Fragments arranged by size:
3290 1910 1469 1396 1183 998 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2394 5037 7494 9064 9251 1700
Size: 110 584 2643 2457 1570 187 2869
Fragments arranged by size:
2869 2643 2457 1570 584 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10420
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10420
BssSI C'ACGA_G
Cuts at: 4521 8480 9492 4521
Size: 3959 1012 5449
Fragments arranged by size:
5449 3959 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2060 3904 4133 4699 5486 7660 794
Size: 1266 1844 229 566 787 2174 3554
Fragments arranged by size:
3554 2174 1844 1266 787 566 229
BstBI TT'CG_AA
Cuts at: 9224 9224
Size: 10420
BstEII G'GTnAC_C
Cuts at: 1184 4366 1184
Size: 3182 7238
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 4736 5161 6139 6205 6751 2011
Size: 2725 425 978 66 546 5680
Fragments arranged by size:
5680 2725 978 546 425 66
BstYI r'GATC_y
Cuts at: 1972 2594 3924 4767 4791 4821 6334 7750 8117
Size: 622 1330 843 24 30 1513 1416 367
Cuts at: 8117 8502 8519 9971 10057 10069 10415 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1513 1452 1416 1330 843 622 385
367 346 86 30 24 17 12
Bsu36I CC'TnA_GG
Cuts at: 2065 2089 4035 5439 5673 6882 2065
Size: 24 1946 1404 234 1209 5603
Fragments arranged by size:
5603 1946 1404 1209 234 24
BtgI C'CryG_G
Cuts at: 513 1065 2004 2475 7099 513
Size: 552 939 471 4624 3834
Fragments arranged by size:
4624 3834 939 552 471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9681
Fragments arranged by size:
9681 683 56
BtsI GCAGTG_nn'
Cuts at: 2641 3567 3603 3905 5544 6558 7936 8730 8750
Size: 926 36 302 1639 1014 1378 794 20
Cuts at: 8750 2641
Size: 4311
Fragments arranged by size:
4311 1639 1378 1014 926 794 302 36
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3662 3697 529
Size: 35 3098 35 7252
Fragments arranged by size:
7252 3098 35 35
DraI TTT'AAA
Cuts at: 7724 7724
Size: 10420
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4665 4703 9427 809
Size: 507 3349 38 4724 1802
Fragments arranged by size:
4724 3349 1802 507 38
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4028 4407 5064
Size: 54 1425 50 275 18 2198 379 657
Cuts at: 5064 5203 6824 7228 7276 7867 8756 10385 8
Size: 139 1621 404 48 591 889 1629 43
Fragments arranged by size:
2198 1629 1621 1425 889 657 591 404
379 275 139 54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7867 1537
Size: 275 6055 4090
Fragments arranged by size:
6055 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2724 3576 3752 5163 5172 5212 5513 6133
Size: 1371 852 176 1411 9 40 301 620
Cuts at: 6133 8350 1353
Size: 2217 3423
Fragments arranged by size:
3423 2217 1411 1371 852 620 301 176
40 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3700 3809 9007 9381 9527 387
Size: 1064 138 2111 109 5198 374 146 1280
Fragments arranged by size:
5198 2111 1280 1064 374 146 138 109
EcoICRI GAG'CTC
Cuts at: 4948 4948
Size: 10420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2218 2661 2765 2921 3040 3192
Size: 393 555 31 443 104 156 119 152
Cuts at: 3192 3315 3339 3375 3399 3453 3612 3817 3823
Size: 123 24 36 24 54 159 205 6
Cuts at: 3823 4112 4328 4736 5012 5106 5651 5699 5705
Size: 289 216 408 276 94 545 48 6
Cuts at: 5705 5711 5768 6099 6198 6596 6866 7016 7656
Size: 6 57 331 99 398 270 150 640
Cuts at: 7656 8483 9080 9867 10135 1239
Size: 827 597 787 268 1524
Fragments arranged by size:
1524 827 787 640 597 555 545 443
408 398 393 331 289 276 270 268
216 205 159 156 152 150 123 119
104 99 94 57 54 48 36 31
24 24 6 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2088 3123 4652 5354 2088
Size: 1035 1529 702 7154
Fragments arranged by size:
7154 1529 1035 702
EcoO109I rG'GnC_Cy
Cuts at: 2098 2329 4645 6364 6853 7325 2098
Size: 231 2316 1719 489 472 5193
Fragments arranged by size:
5193 2316 1719 489 472 231
EcoRI G'AATT_C
Cuts at: 7196 7729 8107 7196
Size: 533 378 9509
Fragments arranged by size:
9509 533 378
EcoRV GAT'ATC
Cuts at: 6691 6903 6691
Size: 212 10208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3680 3680
Size: 10420
FspI TGC'GCA
Cuts at: 3698 4880 8926 3698
Size: 1182 4046 5192
Fragments arranged by size:
5192 4046 1182
FspAI rTGC'GCAy
Cuts at: 3698 3698
Size: 10420
HaeII r_GCGC'y
Cuts at: 1678 4423 5269 6940 9567 1678
Size: 2745 846 1671 2627 2531
Fragments arranged by size:
2745 2627 2531 1671 846
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3236 3268 3914 3946 4769
Size: 32 135 32 1664 32 646 32 823
Cuts at: 4769 4799 4801 4831 4985 5017 5930 5962 6319
Size: 30 2 30 154 32 913 32 357
Cuts at: 6319 6351 6904 6936 7418 7450 9061 9093 9135
Size: 32 553 32 482 32 1611 32 42
Cuts at: 9135 9167 1373
Size: 32 2626
Fragments arranged by size:
2626 1664 1611 913 823 646 553 482
357 154 135 42 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3965 7027 7764 8010 669
Size: 988 281 1524 503 3062 737 246 3079
Fragments arranged by size:
3079 3062 1524 988 737 503 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10420
HpaI GTT'AAC
Cuts at: 8010 8010
Size: 10420
KasI G'GCGC_C
Cuts at: 6936 6936
Size: 10420
KpnI G_GTAC'C
Cuts at: 2466 6808 7495 7745 2466
Size: 4342 687 250 5141
Fragments arranged by size:
5141 4342 687 250
MfeI C'AATT_G
Cuts at: 8019 8019
Size: 10420
MluI A'CGCG_T
Cuts at: 10296 10296
Size: 10420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3545 3697 3838 5562 6327 6699 9534 9718
Size: 2240 152 141 1724 765 372 2835 184
Cuts at: 9718 1305
Size: 2007
Fragments arranged by size:
2835 2240 2007 1724 765 372 184 152
141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4409 5066 6826 7230 10
Size: 54 1425 343 2577 657 1760 404 3200
Fragments arranged by size:
3200 2577 1760 1425 657 404 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2649 7392 8378 8737 8896 518
Size: 648 843 640 4743 986 359 159 2042
Fragments arranged by size:
4743 2042 986 843 648 640 359 159
MspA1I CmG'CkG
Cuts at: 1606 3512 5195 6280 6458 6583 6994 7683 7836
Size: 1906 1683 1085 178 125 411 689 153
Cuts at: 7836 8513 9661 9906 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1906 1683 1148 1085 689 677 411
245 178 153 125
NaeI GCC'GGC
Cuts at: 2396 5039 2396
Size: 2643 7777
NarI GG'CG_CC
Cuts at: 6937 6937
Size: 10420
NcoI C'CATG_G
Cuts at: 513 1065 2004 2475 513
Size: 552 939 471 8458
Fragments arranged by size:
8458 939 552 471
NdeI CA'TA_TG
Cuts at: 387 4846 6907 387
Size: 4459 2061 3900
Fragments arranged by size:
4459 3900 2061
NgoMIV G'CCGG_C
Cuts at: 2394 5037 2394
Size: 2643 7777
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10420
NotI GC'GGCC_GC
Cuts at: 7867 7867
Size: 10420
NspI r_CATG'y
Cuts at: 1132 1229 6624 7041 7778 9323 1132
Size: 97 5395 417 737 1545 2229
Fragments arranged by size:
5395 2229 1545 737 417 97
PasI CC'CwG_GG
Cuts at: 1348 5350 1348
Size: 4002 6418
PciI A'CATG_T
Cuts at: 6620 7037 9319 6620
Size: 417 2282 7721
Fragments arranged by size:
7721 2282 417
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2936 7011 7226 1108
Size: 1828 4075 215 4302
Fragments arranged by size:
4302 4075 1828 215
PfoI T'CCnGG_A
Cuts at: 5745 5884 6384 5745
Size: 139 500 9781
Fragments arranged by size:
9781 500 139
PmeI GTTT'AAAC
Cuts at: 7724 7724
Size: 10420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5116 5148 8489 8521 10002 10034 5116
Size: 32 3341 32 1481 32 5502
Fragments arranged by size:
5502 3341 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2329 4645 2329
Size: 2316 8104
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10420
PsiI TTA'TAA
Cuts at: 7990 7990
Size: 10420
PspOMI G'GGCC_C
Cuts at: 5565 5565
Size: 10420
PspXI vC'TCGA_Gb
Cuts at: 6947 6947
Size: 10420
PstI C_TGCA'G
Cuts at: 830 4653 6028 6606 7772 830
Size: 3823 1375 578 1166 3478
Fragments arranged by size:
3823 3478 1375 1166 578
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10420
PvuII CAG'CTG
Cuts at: 3512 5195 6280 6458 6994 3512
Size: 1683 1085 178 536 6938
Fragments arranged by size:
6938 1683 1085 536 178
SacI G_AGCT'C
Cuts at: 4950 4950
Size: 10420
SalI G'TCGA_C
Cuts at: 1936 3963 7762 1936
Size: 2027 3799 4594
Fragments arranged by size:
4594 3799 2027
SapI GCTCTTCn'nnn_
Cuts at: 2724 2724
Size: 10420
SbfI CC_TGCA'GG
Cuts at: 4653 7772 4653
Size: 3119 7301
ScaI AGT'ACT
Cuts at: 8668 10380 8668
Size: 1712 8708
SexAI A'CCwGG_T
Cuts at: 3734 3734
Size: 10420
SfcI C'TryA_G
Cuts at: 826 1045 3378 4649 5008 5187 5365 6024 6602
Size: 219 2333 1271 359 179 178 659 578
Cuts at: 6602 6894 7768 8903 9584 9775 826
Size: 292 874 1135 681 191 1471
Fragments arranged by size:
2333 1471 1271 1135 874 681 659 578
359 292 219 191 179 178
SfoI GGC'GCC
Cuts at: 6938 6938
Size: 10420
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10420
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10420
SmaI CCC'GGG
Cuts at: 1872 5564 5596 7747 1872
Size: 3692 32 2151 4545
Fragments arranged by size:
4545 3692 2151 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 2996 5618 5961 6359 6947
Size: 197 926 381 672 2622 343 398 588
Cuts at: 6947 8524 9425 9687 820
Size: 1577 901 262 1553
Fragments arranged by size:
2622 1577 1553 926 901 672 588 398
381 343 262 197
SnaBI TAC'GTA
Cuts at: 493 8211 493
Size: 7718 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10420
SphI G_CATG'C
Cuts at: 1132 7778 1132
Size: 6646 3774
SrfI GCCC'GGGC
Cuts at: 5564 5564
Size: 10420
SspI AAT'ATT
Cuts at: 5 52 8344 5
Size: 47 8292 2081
Fragments arranged by size:
8292 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10420
StyI C'CwwG_G
Cuts at: 513 1065 2004 2383 2475 3124 4005 4195 4266
Size: 552 939 379 92 649 881 190 71
Cuts at: 4266 5609 6138 7859 513
Size: 1343 529 1721 3074
Fragments arranged by size:
3074 1721 1343 939 881 649 552 529
379 190 92 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2963 3669 3892 4673 4898 7080 8447
Size: 1255 1383 706 223 781 225 2182 1367
Cuts at: 8447 8464 8617 8802 9247 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2182 1498 1383 1367 1255 781 706 445
225 223 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7163 8666 10378
Size: 274 80 33 51 1443 5186 1503 1712
Cuts at: 10378 96
Size: 138
Fragments arranged by size:
5186 1712 1503 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10380 10412 10380
Size: 32 10388
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10420
XbaI T'CTAG_A
Cuts at: 3559 7756 3559
Size: 4197 6223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3574 7152 3574
Size: 3578 6842
XhoI C'TCGA_G
Cuts at: 1943 6947 1943
Size: 5004 5416
XmaI C'CCGG_G
Cuts at: 1870 5562 5594 7745 1870
Size: 3692 32 2151 4545
Fragments arranged by size:
4545 3692 2151 32
XmnI GAAnn'nnTTC
Cuts at: 3657 8549 3657
Size: 4892 5528
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5372 8228 276
Size: 53 83 186 936 3838 2856 2468
Fragments arranged by size:
3838 2856 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BfrBI BsiWI BstZ17I DraIII FseI NruI
NsiI PacI PmlI PsrI RsrII SacII SanDI SfiI
SwaI