Restriction map for
pFN21AE0091
(Circular) (Six-base) MAPSORT of: pFN21AE0091.seq Check: 522 from: 1 to: 6601
pFN21AE0091 6601 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4411 278
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 2766 3944 1937
Size: 813 16 1178 4594
Fragments arranged by size:
4594 1178 813 16
Acc65I G'GTAC_C
Cuts at: 3097 3922 3097
Size: 825 5776
AclI AA'CG_TT
Cuts at: 1563 4728 5101 1563
Size: 3165 373 3063
Fragments arranged by size:
3165 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4664 6048 6316 1632
Size: 3032 1384 268 1917
Fragments arranged by size:
3032 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6404
AflIII A'CryG_T
Cuts at: 1203 2220 2363 3276 5500 6477 1203
Size: 1017 143 913 2224 977 1327
Fragments arranged by size:
2224 1327 1017 977 913 143
AgeI A'CCGG_T
Cuts at: 3486 5432 3486
Size: 1946 4655
AhdI GACnn_n'nnGTC
Cuts at: 5330 5330
Size: 6601
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6601
AlwNI CAG_nnn'CTG
Cuts at: 2560 3003 4344 5916 2560
Size: 443 1341 1572 3245
Fragments arranged by size:
3245 1572 1341 443
ApaLI G'TGCA_C
Cuts at: 4658 5814 4658
Size: 1156 5445
ApoI r'AATT_y
Cuts at: 1493 2304 3910 4136 4288 6397 1493
Size: 811 1606 226 152 2109 1697
Fragments arranged by size:
2109 1697 1606 811 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5155 160
Size: 872 4123 1606
Fragments arranged by size:
4123 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
AvaI C'yCGr_G
Cuts at: 1870 1943 2831 3746 3926 1870
Size: 73 888 915 180 4545
Fragments arranged by size:
4545 915 888 180 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2713 2746 2713
Size: 33 6568
BamHI G'GATC_C
Cuts at: 3931 4298 3931
Size: 367 6234
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 2104 3032 3097 3922
Size: 325 217 627 284 33 928 65 825
Cuts at: 3922 5377 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 928 825 627 325 284 217
65 33
BanII G_rGCy'C
Cuts at: 1391 3815 3920 1391
Size: 2424 105 4072
Fragments arranged by size:
4072 2424 105
BbeI G_GCGC'C
Cuts at: 2075 2108 2075
Size: 33 6568
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3513 3734 928
Size: 971 1614 221 3795
Fragments arranged by size:
3795 1614 971 221
BbvCI CC'TCA_GC
Cuts at: 2049 3108 2049
Size: 1059 5542
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3022 3056 3445 3479 4792 4826 1903
Size: 34 1085 34 389 34 1313 34 3678
Fragments arranged by size:
3678 1313 1085 389 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4493 5703 4493
Size: 1210 5391
BclI T'GATC_A
Cuts at: 1548 3555 3600 1548
Size: 2007 45 4549
Fragments arranged by size:
4549 2007 45
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5212 136
Size: 107 122 71 631 605 337 3203 1525
Fragments arranged by size:
3203 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2919 3781 6596 2919
Size: 862 2815 2924
Fragments arranged by size:
2924 2815 862
BlpI GC'TnA_GC
Cuts at: 2186 4018 2186
Size: 1832 4769
Bme1580I G_kGCm'C
Cuts at: 1790 4662 5818 1790
Size: 2872 1156 2573
Fragments arranged by size:
2872 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6601
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4332 5290 442
Size: 416 53 83 399 24 2915 958 1753
Fragments arranged by size:
2915 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2342 3740 5261 1239
Size: 1103 1398 1521 2579
Fragments arranged by size:
2579 1521 1398 1103
Bpu10I CC'TnA_GC
Cuts at: 2049 2477 3108 2049
Size: 428 631 5542
Fragments arranged by size:
5542 631 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3602 3836 4726 5591 5889 3602
Size: 234 890 865 298 4314
Fragments arranged by size:
4314 890 865 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5264 882
Size: 617 3765 2219
Fragments arranged by size:
3765 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2281 2364 2770 4392 493
Size: 711 1077 83 406 1622 2702
Fragments arranged by size:
2702 1622 1077 711 406 83
BsaBI GATnn'nnATC
Cuts at: 3212 3587 3713 6595 3212
Size: 375 126 2882 3218
Fragments arranged by size:
3218 2882 375 126
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2072 2105 4408 4790
Size: 53 83 186 936 539 33 2303 382
Cuts at: 4790 5425 275
Size: 635 1451
Fragments arranged by size:
2303 1451 936 635 539 382 186 83
53 33
BsaWI w'CCGG_w
Cuts at: 3294 3486 5034 5432 5706 5853 6264 3294
Size: 192 1548 398 274 147 411 3631
Fragments arranged by size:
3631 1548 411 398 274 192 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6376
Fragments arranged by size:
6376 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2271 1186
Size: 880 205 5516
Fragments arranged by size:
5516 880 205
BseYI C'CCAG_C
Cuts at: 2212 2679 3047 3468 3643 3773 5804 6300 2212
Size: 467 368 421 175 130 2031 496 2513
Fragments arranged by size:
2513 2031 496 467 421 368 175 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6028
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4051 4812 5840 1540
Size: 275 186 2050 761 1028 2301
Fragments arranged by size:
2301 2050 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2631 2664 4662 4747 5818 2631
Size: 33 1998 85 1071 3414
Fragments arranged by size:
3414 1998 1071 85 33
BsiWI C'GTAC_G
Cuts at: 3084 3084
Size: 6601
BsmI GAATG_Cn'
Cuts at: 2264 2602 4112 4205 2264
Size: 338 1510 93 4660
Fragments arranged by size:
4660 1510 338 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3884 3884
Size: 6601
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2631 2664 3815 3920 4662 4747 5818
Size: 399 841 33 1151 105 742 85 1071
Cuts at: 5818 1391
Size: 2174
Fragments arranged by size:
2174 1151 1071 841 742 399 105 85
33
BspEI T'CCGG_A
Cuts at: 3294 6264 3294
Size: 2970 3631
BspHI T'CATG_A
Cuts at: 3204 3558 4490 6220 3204
Size: 354 932 1730 3585
Fragments arranged by size:
3585 1730 932 354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3956 844
Size: 3112 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2960 3703 4488 1394
Size: 429 1137 743 785 3507
Fragments arranged by size:
3507 1137 785 743 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2041 3102 5096 5270 65
Size: 1183 793 1061 1994 174 1396
Fragments arranged by size:
1994 1396 1183 1061 793 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3306 3486 3522 5245 5432 1700
Size: 110 1496 180 36 1723 187 2869
Fragments arranged by size:
2869 1723 1496 187 180 110 36
BsrGI T'GTAC_A
Cuts at: 96 3384 96
Size: 3288 3313
BssHII G'CGCG_C
Cuts at: 1925 2111 1925
Size: 186 6415
BssSI C'ACGA_G
Cuts at: 2147 3359 4661 5673 2147
Size: 1212 1302 1012 3075
Fragments arranged by size:
3075 1302 1212 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6601
BstBI TT'CG_AA
Cuts at: 5405 5405
Size: 6601
BstEII G'GTnAC_C
Cuts at: 1184 3160 3259 3302 1184
Size: 1976 99 43 4483
Fragments arranged by size:
4483 1976 99 43
BstXI CCAn_nnnn'nTGG
Cuts at: 2944 3043 2944
Size: 99 6502
BstYI r'GATC_y
Cuts at: 1972 2919 3570 3582 3708 3781 3931 4298 4683
Size: 947 651 12 126 73 150 367 385
Cuts at: 4683 4700 6152 6238 6250 6596 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 947 651 385 367 346 150
126 86 73 17 12 12
BstZ17I GTA'TAC
Cuts at: 2767 2767
Size: 6601
BtgI C'CryG_G
Cuts at: 513 1065 3267 3815 513
Size: 552 2202 548 3299
Fragments arranged by size:
3299 2202 552 548
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3853 487
Size: 683 56 2627 3235
Fragments arranged by size:
3235 2627 683 56
BtsI GCAGTG_nn'
Cuts at: 3740 4117 4911 4931 3740
Size: 377 794 20 5410
Fragments arranged by size:
5410 794 377 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6566
DraI TTT'AAA
Cuts at: 3905 3905
Size: 6601
DraIII CAC_nnn'GTG
Cuts at: 3723 3723
Size: 6601
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2141 2894 5608 809
Size: 507 825 753 2714 1802
Fragments arranged by size:
2714 1802 825 753 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 2193 2640
Size: 54 1425 50 275 18 325 38 447
Cuts at: 2640 3441 3524 4048 4937 6566 8
Size: 801 83 524 889 1629 43
Fragments arranged by size:
1629 1425 889 801 524 447 325 275
83 54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 4048 1537
Size: 275 2236 4090
Fragments arranged by size:
4090 2236 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3440 4531 1353
Size: 2087 1091 3423
Fragments arranged by size:
3423 2087 1091
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 5188 5562 5708 387
Size: 1064 138 438 3161 374 146 1280
Fragments arranged by size:
3161 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 3740 4664 5261 6048 6316 1239
Size: 393 710 1398 924 597 787 268 1524
Fragments arranged by size:
1524 1398 924 787 710 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2635 2835 3125 2635
Size: 200 290 6111
Fragments arranged by size:
6111 290 200
EcoRI G'AATT_C
Cuts at: 2304 3910 4288 2304
Size: 1606 378 4617
Fragments arranged by size:
4617 1606 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 6601
FspI TGC'GCA
Cuts at: 5107 5107
Size: 6601
HaeII r_GCGC'y
Cuts at: 1678 2024 2075 2108 2230 5748 1678
Size: 346 51 33 122 3518 2531
Fragments arranged by size:
3518 2531 346 122 51 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5242 5274 5316 5348 1373
Size: 32 135 32 3670 32 42 32 2626
Fragments arranged by size:
3670 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3765 3945 4191 669
Size: 988 281 1431 396 180 246 3079
Fragments arranged by size:
3079 1431 988 396 281 246 180
HindIII A'AGCT_T
Cuts at: 748 3800 748
Size: 3052 3549
HpaI GTT'AAC
Cuts at: 3369 4191 3369
Size: 822 5779
KasI G'GCGC_C
Cuts at: 2071 2104 2071
Size: 33 6568
KpnI G_GTAC'C
Cuts at: 3101 3926 3101
Size: 825 5776
MfeI C'AATT_G
Cuts at: 4200 4200
Size: 6601
MluI A'CGCG_T
Cuts at: 6477 6477
Size: 6601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2348 2910 5715 5899 1305
Size: 1043 562 2805 184 2007
Fragments arranged by size:
2805 2007 1043 562 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2642 3443 10
Size: 54 1425 343 810 801 3168
Fragments arranged by size:
3168 1425 810 801 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2433 3167 3719 4559 4918 5077 518
Size: 648 1267 734 552 840 359 159 2042
Fragments arranged by size:
2042 1267 840 734 648 552 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 3030 3184 4017 4694 5842 6087 1606
Size: 488 936 154 833 677 1148 245 2120
Fragments arranged by size:
2120 1148 936 833 677 488 245 154
NarI GG'CG_CC
Cuts at: 2072 2105 2072
Size: 33 6568
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6049
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6601
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6601
NotI GC'GGCC_GC
Cuts at: 4048 4048
Size: 6601
NspI r_CATG'y
Cuts at: 1132 1229 3280 3385 3959 5504 1132
Size: 97 2051 105 574 1545 2229
Fragments arranged by size:
2229 2051 1545 574 105 97
PasI CC'CwG_GG
Cuts at: 1348 2029 2505 3566 1348
Size: 681 476 1061 4383
Fragments arranged by size:
4383 1061 681 476
PciI A'CATG_T
Cuts at: 3276 5500 3276
Size: 2224 4377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6601
PfoI T'CCnGG_A
Cuts at: 2319 2319
Size: 6601
PmeI GTTT'AAAC
Cuts at: 3905 3905
Size: 6601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 6183 6215 4670
Size: 32 1481 32 5056
Fragments arranged by size:
5056 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2635 2635
Size: 6601
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6601
PsiI TTA'TAA
Cuts at: 2973 2982 4171 2973
Size: 9 1189 5403
Fragments arranged by size:
5403 1189 9
PstI C_TGCA'G
Cuts at: 830 2090 2595 3953 830
Size: 1260 505 1358 3478
Fragments arranged by size:
3478 1358 1260 505
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6601
SalI G'TCGA_C
Cuts at: 1936 3943 1936
Size: 2007 4594
SbfI CC_TGCA'GG
Cuts at: 3953 3953
Size: 6601
ScaI AGT'ACT
Cuts at: 4849 6561 4849
Size: 1712 4889
SfcI C'TryA_G
Cuts at: 826 1045 2086 2575 2591 2751 2995 3949 5084
Size: 219 1041 489 16 160 244 954 1135
Cuts at: 5084 5765 5956 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1041 954 681 489 244 219
191 160 16
SfoI GGC'GCC
Cuts at: 2073 2106 2073
Size: 33 6568
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6601
SmaI CCC'GGG
Cuts at: 1872 2833 3928 1872
Size: 961 1095 4545
Fragments arranged by size:
4545 1095 961
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3617 3851 4705 5606 5868 820
Size: 197 926 1674 234 854 901 262 1553
Fragments arranged by size:
1674 1553 926 901 854 262 234 197
SnaBI TAC'GTA
Cuts at: 493 4392 493
Size: 3899 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6601
SphI G_CATG'C
Cuts at: 1132 3959 1132
Size: 2827 3774
SspI AAT'ATT
Cuts at: 5 52 4525 5
Size: 47 4473 2081
Fragments arranged by size:
4473 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6601
StyI C'CwwG_G
Cuts at: 513 1065 3153 3285 4040 513
Size: 552 2088 132 755 3074
Fragments arranged by size:
3074 2088 755 552 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3460 4628 4645 4798 4983 5428 325
Size: 1255 1880 1168 17 153 185 445 1498
Fragments arranged by size:
1880 1498 1255 1168 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 3384 3606
Size: 274 80 33 51 1443 462 945 222
Cuts at: 3606 3621 4847 6559 96
Size: 15 1226 1712 138
Fragments arranged by size:
1712 1443 1226 945 462 274 222 138
80 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6561 6593 6561
Size: 32 6569
Tth111I GACn'n_nGTC
Cuts at: 1316 2746 1316
Size: 1430 5171
XbaI T'CTAG_A
Cuts at: 3937 3937
Size: 6601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3109 3109
Size: 6601
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6601
XmaI C'CCGG_G
Cuts at: 1870 2831 3926 1870
Size: 961 1095 4545
Fragments arranged by size:
4545 1095 961
XmnI GAAnn'nnTTC
Cuts at: 4730 4730
Size: 6601
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4409 276
Size: 53 83 186 936 2875 2468
Fragments arranged by size:
2875 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BplI
Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI