Restriction map for
pFN21AE0090
(Circular) (Six-base) MAPSORT of: pFN21AE0090.seq Check: 1535 from: 1 to: 6154
pFN21AE0090 6154 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3198 3497 1937
Size: 1261 299 4594
Fragments arranged by size:
4594 1261 299
Acc65I G'GTAC_C
Cuts at: 3475 3475
Size: 6154
AclI AA'CG_TT
Cuts at: 1563 4281 4654 1563
Size: 2718 373 3063
Fragments arranged by size:
3063 2718 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3321 4217 5601 5869 1632
Size: 1689 896 1384 268 1917
Fragments arranged by size:
1917 1689 1384 896 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 5053 6030 1203
Size: 3850 977 1327
Fragments arranged by size:
3850 1327 977
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 4883 4883
Size: 6154
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3204 1184
Size: 2020 4134
AlwNI CAG_nnn'CTG
Cuts at: 2103 3897 5469 2103
Size: 1794 1572 2788
Fragments arranged by size:
2788 1794 1572
ApaLI G'TGCA_C
Cuts at: 4211 5367 4211
Size: 1156 4998
ApoI r'AATT_y
Cuts at: 1493 2684 2994 3308 3463 3689 3841 5950 1493
Size: 1191 310 314 155 226 152 2109 1697
Fragments arranged by size:
2109 1697 1191 314 310 226 155 152
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 2559 3479 1870
Size: 73 616 920 4545
Fragments arranged by size:
4545 920 616 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2117 2150 2720 2753 2117
Size: 33 570 33 5518
Fragments arranged by size:
5518 570 33 33
BamHI G'GATC_C
Cuts at: 2799 3484 3851 2799
Size: 685 367 5102
Fragments arranged by size:
5102 685 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3475 4930 618
Size: 325 217 627 1688 1455 1842
Fragments arranged by size:
1842 1688 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2940 3473 1391
Size: 1549 533 4072
Fragments arranged by size:
4072 1549 533
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2029 3224 928
Size: 971 130 1195 3858
Fragments arranged by size:
3858 1195 971 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4345 4379 1903
Size: 34 2408 34 3678
Fragments arranged by size:
3678 2408 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4046 5256 4046
Size: 1210 4944
BclI T'GATC_A
Cuts at: 1548 2286 1548
Size: 738 5416
BfrBI ATG'CAT
Cuts at: 2579 2579
Size: 6154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2444 4765 136
Size: 107 122 71 631 605 772 2321 1525
Fragments arranged by size:
2321 1525 772 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6149 6149
Size: 6154
BlpI GC'TnA_GC
Cuts at: 3571 3571
Size: 6154
Bme1580I G_kGCm'C
Cuts at: 1790 3077 4215 5371 1790
Size: 1287 1138 1156 2573
Fragments arranged by size:
2573 1287 1156 1138
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3885 4843 442
Size: 416 53 83 399 24 2468 958 1753
Fragments arranged by size:
2468 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 2470 2610 2881 2986 4814 1239
Size: 886 345 140 271 105 1828 2579
Fragments arranged by size:
2579 1828 886 345 271 140 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4279 5144 5442 4279
Size: 865 298 4991
Fragments arranged by size:
4991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4817 882
Size: 617 3318 2219
Fragments arranged by size:
3318 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3175 3945 493
Size: 711 1971 770 2702
Fragments arranged by size:
2702 1971 770 711
BsaBI GATnn'nnATC
Cuts at: 2541 6148 2541
Size: 3607 2547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3961 4343 4978 275
Size: 53 83 186 936 2428 382 635 1451
Fragments arranged by size:
2428 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2955 4587 4985 5259 5406 5817 2955
Size: 1632 398 274 147 411 3292
Fragments arranged by size:
3292 1632 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5929
Fragments arranged by size:
5929 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2619 3129 1186
Size: 1433 510 4211
Fragments arranged by size:
4211 1433 510
BseYI C'CCAG_C
Cuts at: 2926 3002 3027 5357 5853 2926
Size: 76 25 2330 496 3227
Fragments arranged by size:
3227 2330 496 76 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3197 1163
Size: 573 1461 4120
Fragments arranged by size:
4120 1461 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3604 4365 5393 1540
Size: 275 186 1603 761 1028 2301
Fragments arranged by size:
2301 1603 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 2940 4215 4300 5371 2016
Size: 924 1275 85 1071 2799
Fragments arranged by size:
2799 1275 1071 924 85
BsmI GAATG_Cn'
Cuts at: 3665 3758 3665
Size: 93 6061
BsmBI CGTCTCn'nnnn_
Cuts at: 2962 2962
Size: 6154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2940 3077 3473 4215 4300 5371
Size: 399 226 924 137 396 742 85 1071
Cuts at: 5371 1391
Size: 2174
Fragments arranged by size:
2174 1071 924 742 399 396 226 137
85
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 2680 4043 5773 2680
Size: 1363 1730 3061
Fragments arranged by size:
3061 1730 1363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2965 3509 844
Size: 2121 544 3489
Fragments arranged by size:
3489 2121 544
BsrBI CCG'CTC
Cuts at: 1394 1823 4041 1394
Size: 429 2218 3507
Fragments arranged by size:
3507 2218 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4649 4823 65
Size: 1183 3401 174 1396
Fragments arranged by size:
3401 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4798 4985 1700
Size: 110 2988 187 2869
Fragments arranged by size:
2988 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 4214 5226 4214
Size: 1012 5142
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6154
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6154
BstYI r'GATC_y
Cuts at: 1972 2799 3484 3851 4236 4253 5705 5791 5803
Size: 827 685 367 385 17 1452 86 12
Cuts at: 5803 6149 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 827 685 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3203 3203
Size: 6154
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5602
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5415
Fragments arranged by size:
5415 683 56
BtsI GCAGTG_nn'
Cuts at: 2064 2433 3075 3670 4464 4484 2064
Size: 369 642 595 794 20 3734
Fragments arranged by size:
3734 794 642 595 369 20
ClaI AT'CG_AT
Cuts at: 1554 3333 1554
Size: 1779 4375
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6119
DraI TTT'AAA
Cuts at: 3458 3458
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5161 809
Size: 507 3845 1802
Fragments arranged by size:
3845 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 2689 3325
Size: 54 1425 50 275 18 489 370 636
Cuts at: 3325 3601 4490 6119 8
Size: 276 889 1629 43
Fragments arranged by size:
1629 1425 889 636 489 370 276 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3601 1537
Size: 275 1789 4090
Fragments arranged by size:
4090 1789 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2339 2951 4084 1353
Size: 986 612 1133 3423
Fragments arranged by size:
3423 1133 986 612
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4741 5115 5261 387
Size: 1064 138 3152 374 146 1280
Fragments arranged by size:
3152 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2938 2938
Size: 6154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2470 2610 2881 2986 3321 4217
Size: 393 493 345 140 271 105 335 896
Cuts at: 4217 4814 5601 5869 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 896 787 597 493 393 345 335
271 268 140 105
EcoNI CCTnn'n_nnAGG
Cuts at: 2095 2126 2592 2095
Size: 31 466 5657
Fragments arranged by size:
5657 466 31
EcoO109I rG'GnC_Cy
Cuts at: 2100 3095 3134 2100
Size: 995 39 5120
Fragments arranged by size:
5120 995 39
EcoRI G'AATT_C
Cuts at: 2994 3463 3841 2994
Size: 469 378 5307
Fragments arranged by size:
5307 469 378
EcoRV GAT'ATC
Cuts at: 2511 2511
Size: 6154
FspI TGC'GCA
Cuts at: 4660 4660
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 2460 5301 1678
Size: 782 2841 2531
Fragments arranged by size:
2841 2531 782
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2760 2792 2885 2917 4795
Size: 32 135 32 1188 32 93 32 1878
Cuts at: 4795 4827 4869 4901 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1878 1188 135 93 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3498 3744 669
Size: 988 281 1560 246 3079
Fragments arranged by size:
3079 1560 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6154
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KpnI G_GTAC'C
Cuts at: 3479 3479
Size: 6154
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5268 5452 1305
Size: 3963 184 2007
Fragments arranged by size:
3963 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 2691 3327 10
Size: 54 1425 343 489 370 636 2837
Fragments arranged by size:
2837 1425 636 489 370 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4112 4471 4630 518
Size: 648 843 2103 359 159 2042
Fragments arranged by size:
2103 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3570 4247 5395 5640 1606
Size: 1964 677 1148 245 2120
Fragments arranged by size:
2120 1964 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5602
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6154
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6154
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NsiI A_TGCA'T
Cuts at: 2581 2581
Size: 6154
NspI r_CATG'y
Cuts at: 1132 1229 3512 5057 1132
Size: 97 2283 1545 2229
Fragments arranged by size:
2283 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2698 1348
Size: 1350 4804
PciI A'CATG_T
Cuts at: 5053 5053
Size: 6154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2103 2552 2698 1108
Size: 995 449 146 4564
Fragments arranged by size:
4564 995 449 146
PfoI T'CCnGG_A
Cuts at: 2587 3440 2587
Size: 853 5301
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PmlI CAC'GTG
Cuts at: 3175 3175
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4223 4255 5736 5768 4223
Size: 32 1481 32 4609
Fragments arranged by size:
4609 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2100 3095 3134 2100
Size: 995 39 5120
Fragments arranged by size:
5120 995 39
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6154
PsiI TTA'TAA
Cuts at: 2748 3724 2748
Size: 976 5178
PstI C_TGCA'G
Cuts at: 830 2071 3506 830
Size: 1241 1435 3478
Fragments arranged by size:
3478 1435 1241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
SacI G_AGCT'C
Cuts at: 2940 2940
Size: 6154
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SapI GCTCTTCn'nnn_
Cuts at: 2951 2951
Size: 6154
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 4402 6114 4402
Size: 1712 4442
SfcI C'TryA_G
Cuts at: 826 1045 2067 3247 3342 3502 4637 5318 5509
Size: 219 1022 1180 95 160 1135 681 191
Cuts at: 5509 826
Size: 1471
Fragments arranged by size:
1471 1180 1135 1022 681 219 191 160
95
SfiI GGCCn_nnn'nGGCC
Cuts at: 2444 2444
Size: 6154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6154
SmaI CCC'GGG
Cuts at: 1872 3481 1872
Size: 1609 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4258 5159 5421 820
Size: 197 926 2315 901 262 1553
Fragments arranged by size:
2315 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 3512 1132
Size: 2380 3774
SspI AAT'ATT
Cuts at: 5 52 2147 4078 5
Size: 47 2095 1931 2081
Fragments arranged by size:
2095 2081 1931 47
StuI AGG'CCT
Cuts at: 1517 2447 2598 1517
Size: 930 151 5073
Fragments arranged by size:
5073 930 151
StyI C'CwwG_G
Cuts at: 513 1065 3167 3593 513
Size: 552 2102 426 3074
Fragments arranged by size:
3074 2102 552 426
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4181 4198 4351 4536 4981 325
Size: 1255 2601 17 153 185 445 1498
Fragments arranged by size:
2601 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2751 4400 6112
Size: 274 80 33 51 1443 774 1649 1712
Cuts at: 6112 96
Size: 138
Fragments arranged by size:
1712 1649 1443 774 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2522 2554 2858 2890 6114 6146 2522
Size: 32 304 32 3224 32 2530
Fragments arranged by size:
3224 2530 304 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6154
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6154
XmaI C'CCGG_G
Cuts at: 1870 3479 1870
Size: 1609 4545
XmnI GAAnn'nnTTC
Cuts at: 2200 2828 4283 2200
Size: 628 1455 4071
Fragments arranged by size:
4071 1455 628
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfoI
SrfI SwaI XcmI