Restriction map for
pFN21AE0088
(Circular) (Six-base) MAPSORT of: pFN21AE0088.seq Check: 1512 from: 1 to: 7420
pFN21AE0088 7420 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5230 278
Size: 53 83 186 936 3694 2468
Fragments arranged by size:
3694 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2560 4763 1937
Size: 623 2203 4594
Fragments arranged by size:
4594 2203 623
Acc65I G'GTAC_C
Cuts at: 3644 4741 3644
Size: 1097 6323
AclI AA'CG_TT
Cuts at: 1563 5547 5920 1563
Size: 3984 373 3063
Fragments arranged by size:
3984 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2982 4631 5483 6867 7135 1632
Size: 1350 1649 852 1384 268 1917
Fragments arranged by size:
1917 1649 1384 1350 852 268
AflII C'TTAA_G
Cuts at: 820 1017 2723 820
Size: 197 1706 5517
Fragments arranged by size:
5517 1706 197
AflIII A'CryG_T
Cuts at: 1203 2871 6319 7296 1203
Size: 1668 3448 977 1327
Fragments arranged by size:
3448 1668 1327 977
AgeI A'CCGG_T
Cuts at: 6251 6251
Size: 7420
AhdI GACnn_n'nnGTC
Cuts at: 6149 6149
Size: 7420
AleI CACnn'nnGTG
Cuts at: 1166 3566 1166
Size: 2400 5020
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7420
AlwNI CAG_nnn'CTG
Cuts at: 2714 2734 5163 6735 2714
Size: 20 2429 1572 3399
Fragments arranged by size:
3399 2429 1572 20
ApaI G_GGCC'C
Cuts at: 3478 3478
Size: 7420
ApaLI G'TGCA_C
Cuts at: 5477 6633 5477
Size: 1156 6264
ApoI r'AATT_y
Cuts at: 1493 3223 4526 4729 4955 5107 7216 1493
Size: 1730 1303 203 226 152 2109 1697
Fragments arranged by size:
2109 1730 1697 1303 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 5974 160
Size: 872 4942 1606
Fragments arranged by size:
4942 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7420
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2927 4418 4745 1870
Size: 73 90 894 1491 327 4545
Fragments arranged by size:
4545 1491 894 327 90 73
AvrII C'CTAG_G
Cuts at: 3610 3610
Size: 7420
BamHI G'GATC_C
Cuts at: 4176 4750 5117 4176
Size: 574 367 6479
Fragments arranged by size:
6479 574 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3066 3644 4741 6196 618
Size: 325 217 627 1279 578 1097 1455 1842
Fragments arranged by size:
1842 1455 1279 1097 627 578 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 2571 3478 3859 4739 1391
Size: 649 531 907 381 880 4072
Fragments arranged by size:
4072 907 880 649 531 381
BbeI G_GCGC'C
Cuts at: 3070 3070
Size: 7420
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 4431 928
Size: 971 645 1887 3917
Fragments arranged by size:
3917 1887 971 645
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2005 2039 2362 2396 5611 5645 1903
Size: 34 68 34 323 34 3215 34 3678
Fragments arranged by size:
3678 3215 323 68 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4682 5312 6522 4682
Size: 630 1210 5580
Fragments arranged by size:
5580 1210 630
BclI T'GATC_A
Cuts at: 1548 3093 1548
Size: 1545 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3968 6031 136
Size: 107 122 71 631 605 2296 2063 1525
Fragments arranged by size:
2296 2063 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7415 7415
Size: 7420
BlpI GC'TnA_GC
Cuts at: 4837 4837
Size: 7420
Bme1580I G_kGCm'C
Cuts at: 1790 2065 3478 4447 5481 6637 1790
Size: 275 1413 969 1034 1156 2573
Fragments arranged by size:
2573 1413 1156 1034 969 275
BmgBI CAC'GTC
Cuts at: 1318 3805 1318
Size: 2487 4933
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3132 4607 5151
Size: 416 53 83 399 24 1715 1475 544
Cuts at: 5151 6109 442
Size: 958 1753
Fragments arranged by size:
1753 1715 1475 958 544 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7420
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4713 4713
Size: 7420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2569 3669 6080 1239
Size: 1330 1100 2411 2579
Fragments arranged by size:
2579 2411 1330 1100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 3175 5545 6410 6708 2636
Size: 539 2370 865 298 3348
Fragments arranged by size:
3348 2370 865 539 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6083 882
Size: 617 4584 2219
Fragments arranged by size:
4584 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2872 3568 5211 493
Size: 711 1668 696 1643 2702
Fragments arranged by size:
2702 1668 1643 711 696
BsaBI GATnn'nnATC
Cuts at: 3168 7414 3168
Size: 4246 3174
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3067 5227 5609 6244
Size: 53 83 186 936 1534 2160 382 635
Cuts at: 6244 275
Size: 1451
Fragments arranged by size:
2160 1534 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3630 5853 6251 6525 6672 7083 3630
Size: 2223 398 274 147 411 3967
Fragments arranged by size:
3967 2223 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2278 2308 1178
Size: 30 165 30 875 30 6290
Fragments arranged by size:
6290 875 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2057 2335 3540 1186
Size: 826 45 278 1205 5066
Fragments arranged by size:
5066 1205 826 278 45
BseYI C'CCAG_C
Cuts at: 2028 2058 3021 6623 7119 2028
Size: 30 963 3602 496 2329
Fragments arranged by size:
3602 2329 963 496 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 1163
Size: 573 645 6202
Fragments arranged by size:
6202 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4870 5631 6659 1540
Size: 275 186 2869 761 1028 2301
Fragments arranged by size:
2869 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 2571 3708 3859 4191 5481 5566 6637 2013
Size: 558 1137 151 332 1290 85 1071 2796
Fragments arranged by size:
2796 1290 1137 1071 558 332 151 85
BsmI GAATG_Cn'
Cuts at: 3621 4931 5024 3621
Size: 1310 93 6017
Fragments arranged by size:
6017 1310 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4684 4684
Size: 7420
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2040 2065 2571 3478 3708 3859
Size: 399 223 27 25 506 907 230 151
Cuts at: 3859 4191 4447 4739 5481 5566 6637 1391
Size: 332 256 292 742 85 1071 2174
Fragments arranged by size:
2174 1071 907 742 506 399 332 292
256 230 223 151 85 27 25
BspEI T'CCGG_A
Cuts at: 7083 7083
Size: 7420
BspHI T'CATG_A
Cuts at: 3603 4128 5309 7039 3603
Size: 525 1181 1730 3984
Fragments arranged by size:
3984 1730 1181 525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4775 844
Size: 3931 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5307 1394
Size: 429 3484 3507
Fragments arranged by size:
3507 3484 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2380 3000 5915 6089 65
Size: 1183 1132 620 2915 174 1396
Fragments arranged by size:
2915 1396 1183 1132 620 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6064 6251 1700
Size: 110 4254 187 2869
Fragments arranged by size:
4254 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2988 3342 96
Size: 2892 354 4174
Fragments arranged by size:
4174 2892 354
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7420
BssSI C'ACGA_G
Cuts at: 5480 6492 5480
Size: 1012 6408
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7420
BstBI TT'CG_AA
Cuts at: 6224 6224
Size: 7420
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7420
BstXI CCAn_nnnn'nTGG
Cuts at: 3322 3693 4447 3322
Size: 371 754 6295
Fragments arranged by size:
6295 754 371
BstYI r'GATC_y
Cuts at: 1972 2491 2800 4176 4750 5117 5502 5519 6971
Size: 519 309 1376 574 367 385 17 1452
Cuts at: 6971 7057 7069 7415 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1376 574 519 385 367 346
309 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2898 3062 513
Size: 552 939 894 164 4871
Fragments arranged by size:
4871 939 894 552 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6681
Fragments arranged by size:
6681 683 56
BtsI GCAGTG_nn'
Cuts at: 3050 3549 4936 5730 5750 3050
Size: 499 1387 794 20 4720
Fragments arranged by size:
4720 1387 794 499 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4210 4245 529
Size: 35 3646 35 3704
Fragments arranged by size:
3704 3646 35 35
DraI TTT'AAA
Cuts at: 4724 4724
Size: 7420
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6427 809
Size: 507 5111 1802
Fragments arranged by size:
5111 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2997 3291 3960
Size: 54 1425 50 275 18 1167 294 669
Cuts at: 3960 4867 5756 7385 8
Size: 907 889 1629 43
Fragments arranged by size:
1629 1425 1167 907 889 669 294 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4867 1537
Size: 275 3055 4090
Fragments arranged by size:
4090 3055 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5350 1353
Size: 3997 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2600 6007 6381 6527 387
Size: 1064 138 1011 3407 374 146 1280
Fragments arranged by size:
3407 1280 1064 1011 374 146 138
EcoICRI GAG'CTC
Cuts at: 2569 3857 2569
Size: 1288 6132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2569 2982 3669 4631 5483 6080 6867
Size: 393 937 413 687 962 852 597 787
Cuts at: 6867 7135 1239
Size: 268 1524
Fragments arranged by size:
1524 962 937 852 787 687 597 413
393 268
EcoO109I rG'GnC_Cy
Cuts at: 2914 3474 4273 2914
Size: 560 799 6061
Fragments arranged by size:
6061 799 560
EcoRI G'AATT_C
Cuts at: 3223 4526 4729 5107 3223
Size: 1303 203 378 5536
Fragments arranged by size:
5536 1303 378 203
FspI TGC'GCA
Cuts at: 5926 5926
Size: 7420
HaeII r_GCGC'y
Cuts at: 1678 3070 6567 1678
Size: 1392 3497 2531
Fragments arranged by size:
3497 2531 1392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2476 2508 2879 2911 6061
Size: 32 135 32 904 32 371 32 3150
Cuts at: 6061 6093 6135 6167 1373
Size: 32 42 32 2626
Fragments arranged by size:
3150 2626 904 371 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4764 5010 669
Size: 988 281 2826 246 3079
Fragments arranged by size:
3079 2826 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7420
HpaI GTT'AAC
Cuts at: 5010 5010
Size: 7420
KasI G'GCGC_C
Cuts at: 3066 3066
Size: 7420
KpnI G_GTAC'C
Cuts at: 3648 4745 3648
Size: 1097 6323
MfeI C'AATT_G
Cuts at: 5019 5019
Size: 7420
MluI A'CGCG_T
Cuts at: 7296 7296
Size: 7420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3252 3832 3892 6534 6718 1305
Size: 1947 580 60 2642 184 2007
Fragments arranged by size:
2642 2007 1947 580 184 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2999 3293 10
Size: 54 1425 343 1167 294 4137
Fragments arranged by size:
4137 1425 1167 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 3566 5378 5737 5896 518
Size: 648 1578 822 1812 359 159 2042
Fragments arranged by size:
2042 1812 1578 822 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2302 2406 2617 2714 3025 3729 4836
Size: 452 244 104 211 97 311 704 1107
Cuts at: 4836 5513 6661 6906 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1107 704 677 452 311 245
244 211 104 97
NarI GG'CG_CC
Cuts at: 3067 3067
Size: 7420
NcoI C'CATG_G
Cuts at: 513 1065 2004 2898 3062 513
Size: 552 939 894 164 4871
Fragments arranged by size:
4871 939 894 552 164
NdeI CA'TA_TG
Cuts at: 387 4380 387
Size: 3993 3427
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7420
NotI GC'GGCC_GC
Cuts at: 4867 4867
Size: 7420
NspI r_CATG'y
Cuts at: 1132 1229 2295 4778 6323 1132
Size: 97 1066 2483 1545 2229
Fragments arranged by size:
2483 2229 1545 1066 97
PasI CC'CwG_GG
Cuts at: 1348 4447 1348
Size: 3099 4321
PciI A'CATG_T
Cuts at: 6319 6319
Size: 7420
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7420
PfoI T'CCnGG_A
Cuts at: 2487 2487
Size: 7420
PmeI GTTT'AAAC
Cuts at: 4724 4724
Size: 7420
PmlI CAC'GTG
Cuts at: 2872 2872
Size: 7420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4554 4586 5489 5521 7002 7034 4554
Size: 32 903 32 1481 32 4940
Fragments arranged by size:
4940 1481 903 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7420
PsiI TTA'TAA
Cuts at: 4990 4990
Size: 7420
PspOMI G'GGCC_C
Cuts at: 3474 3474
Size: 7420
PstI C_TGCA'G
Cuts at: 830 2913 3045 4772 830
Size: 2083 132 1727 3478
Fragments arranged by size:
3478 2083 1727 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7420
PvuII CAG'CTG
Cuts at: 2058 2302 2617 3025 3729 2058
Size: 244 315 408 704 5749
Fragments arranged by size:
5749 704 408 315 244
SacI G_AGCT'C
Cuts at: 2571 3859 2571
Size: 1288 6132
SalI G'TCGA_C
Cuts at: 1936 4762 1936
Size: 2826 4594
SbfI CC_TGCA'GG
Cuts at: 4772 4772
Size: 7420
ScaI AGT'ACT
Cuts at: 2112 2759 5668 7380 2112
Size: 647 2909 1712 2152
Fragments arranged by size:
2909 2152 1712 647
SfcI C'TryA_G
Cuts at: 826 1045 2812 2909 3041 4768 5903 6584 6775
Size: 219 1767 97 132 1727 1135 681 191
Cuts at: 6775 826
Size: 1471
Fragments arranged by size:
1767 1727 1471 1135 681 219 191 132
97
SfoI GGC'GCC
Cuts at: 3068 3068
Size: 7420
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7420
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7420
SmaI CCC'GGG
Cuts at: 1872 2035 4747 1872
Size: 163 2712 4545
Fragments arranged by size:
4545 2712 163
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 2723 3154 5524 6425 6687
Size: 197 926 708 72 431 2370 901 262
Cuts at: 6687 820
Size: 1553
Fragments arranged by size:
2370 1553 926 901 708 431 262 197
72
SnaBI TAC'GTA
Cuts at: 493 5211 493
Size: 4718 2702
SpeI A'CTAG_T
Cuts at: 152 3451 152
Size: 3299 4121
SphI G_CATG'C
Cuts at: 1132 4778 1132
Size: 3646 3774
SrfI GCCC'GGGC
Cuts at: 2035 2035
Size: 7420
SspI AAT'ATT
Cuts at: 5 52 2596 4462 5344 5
Size: 47 2544 1866 882 2081
Fragments arranged by size:
2544 2081 1866 882 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7420
StyI C'CwwG_G
Cuts at: 513 1065 2004 2898 3062 3610 4859 513
Size: 552 939 894 164 548 1249 3074
Fragments arranged by size:
3074 1249 939 894 552 548 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2768 2935 3798 5447 5464 5617 5802
Size: 1255 1188 167 863 1649 17 153 185
Cuts at: 5802 6247 325
Size: 445 1498
Fragments arranged by size:
1649 1498 1255 1188 863 445 185 167
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2110 2757 2988
Size: 274 80 33 51 1443 133 647 231
Cuts at: 2988 3342 3733 5666 7378 96
Size: 354 391 1933 1712 138
Fragments arranged by size:
1933 1712 1443 647 391 354 274 231
138 133 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2278 2310 2861 2893 3002 3034 7380 7412 2278
Size: 32 551 32 109 32 4346 32 2286
Fragments arranged by size:
4346 2286 551 109 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7420
XbaI T'CTAG_A
Cuts at: 4756 4756
Size: 7420
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2306 2621 2306
Size: 315 7105
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7420
XmaI C'CCGG_G
Cuts at: 1870 2033 4745 1870
Size: 163 2712 4545
Fragments arranged by size:
4545 2712 163
XmnI GAAnn'nnTTC
Cuts at: 5549 5549
Size: 7420
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5228 276
Size: 53 83 186 936 3694 2468
Fragments arranged by size:
3694 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SwaI