Restriction map for
pFN21AE0086
(Circular) (Six-base) MAPSORT of: pFN21AE0086.seq Check: 4598 from: 1 to: 6280
pFN21AE0086 6280 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2893 3130 2893
Size: 237 6043
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4090 278
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3623 1937
Size: 1686 4594
Acc65I G'GTAC_C
Cuts at: 3601 3601
Size: 6280
AclI AA'CG_TT
Cuts at: 1563 4407 4780 1563
Size: 2844 373 3063
Fragments arranged by size:
3063 2844 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2663 3307 4343 5727 5995 1632
Size: 1031 644 1036 1384 268 1917
Fragments arranged by size:
1917 1384 1036 1031 644 268
AfeI AGC'GCT
Cuts at: 2321 2616 2321
Size: 295 5985
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6083
AflIII A'CryG_T
Cuts at: 1203 5179 6156 1203
Size: 3976 977 1327
Fragments arranged by size:
3976 1327 977
AgeI A'CCGG_T
Cuts at: 5111 5111
Size: 6280
AhdI GACnn_n'nnGTC
Cuts at: 5009 5009
Size: 6280
AleI CACnn'nnGTG
Cuts at: 1166 2801 3539 1166
Size: 1635 738 3907
Fragments arranged by size:
3907 1635 738
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6280
AlwNI CAG_nnn'CTG
Cuts at: 2899 4023 5595 2899
Size: 1124 1572 3584
Fragments arranged by size:
3584 1572 1124
ApaI G_GGCC'C
Cuts at: 2088 2198 2252 3249 3425 2088
Size: 110 54 997 176 4943
Fragments arranged by size:
4943 997 176 110 54
ApaLI G'TGCA_C
Cuts at: 2360 4337 5493 2360
Size: 1977 1156 3147
Fragments arranged by size:
3147 1977 1156
ApoI r'AATT_y
Cuts at: 1493 3589 3815 3967 6076 1493
Size: 2096 226 152 2109 1697
Fragments arranged by size:
2109 2096 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4834 160
Size: 872 3802 1606
Fragments arranged by size:
3802 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2081 2198 2251 2618 3560 3605
Size: 73 119 19 117 53 367 942 45
Cuts at: 3605 1870
Size: 4545
Fragments arranged by size:
4545 942 367 119 117 73 53 45
19
BamHI G'GATC_C
Cuts at: 3233 3610 3977 3233
Size: 377 367 5536
Fragments arranged by size:
5536 377 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2608 3440 3485 3601 5056
Size: 325 217 627 821 832 45 116 1455
Cuts at: 5056 618
Size: 1842
Fragments arranged by size:
1842 1455 832 821 627 325 217 116
45
BanII G_rGCy'C
Cuts at: 1391 2088 2198 2252 2385 3249 3425 3599 1391
Size: 697 110 54 133 864 176 174 4072
Fragments arranged by size:
4072 864 697 176 174 133 110 54
BbeI G_GCGC'C
Cuts at: 3489 3489
Size: 6280
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5309
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4471 4505 1903
Size: 34 2534 34 3678
Fragments arranged by size:
3678 2534 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4172 5382 4172
Size: 1210 5070
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6280
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2111 4891
Size: 107 122 71 631 605 337 102 2780
Cuts at: 4891 136
Size: 1525
Fragments arranged by size:
2780 1525 631 605 337 122 107 102
71
BglII A'GATC_T
Cuts at: 6275 6275
Size: 6280
BlpI GC'TnA_GC
Cuts at: 2384 3337 3697 2384
Size: 953 360 4967
Fragments arranged by size:
4967 953 360
Bme1580I G_kGCm'C
Cuts at: 1790 2042 2069 2088 2198 2252 2364 3249 3425
Size: 252 27 19 110 54 112 885 176
Cuts at: 3425 4341 5497 1790
Size: 916 1156 2573
Fragments arranged by size:
2573 1156 916 885 252 176 112 110
54 27 19
BmgBI CAC'GTC
Cuts at: 1318 2359 2500 1318
Size: 1041 141 5098
Fragments arranged by size:
5098 1041 141
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2837 4011 4969
Size: 416 53 83 399 24 1420 1174 958
Cuts at: 4969 442
Size: 1753
Fragments arranged by size:
1753 1420 1174 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2750 2834 4940 1239
Size: 1511 84 2106 2579
Fragments arranged by size:
2579 2106 1511 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 3401 4405 5270 5568 2513
Size: 888 1004 865 298 3225
Fragments arranged by size:
3225 1004 888 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4943 882
Size: 617 3444 2219
Fragments arranged by size:
3444 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4071 493
Size: 711 2867 2702
Fragments arranged by size:
2867 2702 711
BsaBI GATnn'nnATC
Cuts at: 6274 6274
Size: 6280
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2177 2757 2909 3486
Size: 53 83 186 936 644 580 152 577
Cuts at: 3486 4087 4469 5104 275
Size: 601 382 635 1451
Fragments arranged by size:
1451 936 644 635 601 580 577 382
186 152 83 53
BsaWI w'CCGG_w
Cuts at: 4713 5111 5385 5532 5943 4713
Size: 398 274 147 411 5050
Fragments arranged by size:
5050 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6055
Fragments arranged by size:
6055 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 2993 1186
Size: 1066 741 4473
Fragments arranged by size:
4473 1066 741
BseYI C'CCAG_C
Cuts at: 2087 3153 5483 5979 2087
Size: 1066 2330 496 2388
Fragments arranged by size:
2388 2330 1066 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 2573 3029 3139 1163
Size: 573 597 240 456 110 4304
Fragments arranged by size:
4304 597 573 456 240 110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2130 3519 3730 4491 5519 1540
Size: 275 186 129 1389 211 761 1028 2301
Fragments arranged by size:
2301 1389 1028 761 275 211 186 129
BsiHKAI G_wGCw'C
Cuts at: 2364 4341 4426 5497 2364
Size: 1977 85 1071 3147
Fragments arranged by size:
3147 1977 1071 85
BsmI GAATG_Cn'
Cuts at: 3791 3884 3791
Size: 93 6187
BsmBI CGTCTCn'nnnn_
Cuts at: 2561 2561
Size: 6280
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2069 2088 2198 2252 2364 2385
Size: 399 252 27 19 110 54 112 21
Cuts at: 2385 3249 3425 3599 4341 4426 5497 1391
Size: 864 176 174 742 85 1071 2174
Fragments arranged by size:
2174 1071 864 742 399 252 176 174
112 110 85 54 27 21 19
BspEI T'CCGG_A
Cuts at: 5943 5943
Size: 6280
BspHI T'CATG_A
Cuts at: 2967 4169 5899 2967
Size: 1202 1730 3348
Fragments arranged by size:
3348 1730 1202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2661 2893 2981 3130 3503 3635 844
Size: 1817 232 88 149 373 132 3489
Fragments arranged by size:
3489 1817 373 232 149 132 88
BsrBI CCG'CTC
Cuts at: 1394 1823 2022 2230 2965 4167 1394
Size: 429 199 208 735 1202 3507
Fragments arranged by size:
3507 1202 735 429 208 199
BsrDI GCAATG_nn'
Cuts at: 65 1248 2626 4775 4949 65
Size: 1183 1378 2149 174 1396
Fragments arranged by size:
2149 1396 1378 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3087 3370 4924 5111 1700
Size: 110 1277 283 1554 187 2869
Fragments arranged by size:
2869 1554 1277 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6280
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6280
BssSI C'ACGA_G
Cuts at: 3069 3467 4340 5352 3069
Size: 398 873 1012 3997
Fragments arranged by size:
3997 1012 873 398
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2899 794
Size: 2105 4175
BstBI TT'CG_AA
Cuts at: 5084 5084
Size: 6280
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6280
BstYI r'GATC_y
Cuts at: 1972 3233 3555 3610 3977 4362 4379 5831 5917
Size: 1261 322 55 367 385 17 1452 86
Cuts at: 5917 5929 6275 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1261 385 367 346 322 86
55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2217 2424 2478 2766 3241 3480
Size: 552 939 213 207 54 288 475 239
Cuts at: 3480 3540 513
Size: 60 3253
Fragments arranged by size:
3253 939 552 475 288 239 213 207
60 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5541
Fragments arranged by size:
5541 683 56
BtsI GCAGTG_nn'
Cuts at: 2522 2748 3796 4590 4610 2522
Size: 226 1048 794 20 4192
Fragments arranged by size:
4192 1048 794 226 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6280
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2467 2502 529
Size: 35 1903 35 4307
Fragments arranged by size:
4307 1903 35 35
DraI TTT'AAA
Cuts at: 3584 3584
Size: 6280
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3469 5287 809
Size: 507 2153 1818 1802
Fragments arranged by size:
2153 1818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2421 2466
Size: 54 1425 50 275 18 297 294 45
Cuts at: 2466 2715 3516 3550 3727 4616 6245 8
Size: 249 801 34 177 889 1629 43
Fragments arranged by size:
1629 1425 889 801 297 294 275 249
177 54 50 45 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 2127 3516 3727 1537
Size: 275 315 1389 211 4090
Fragments arranged by size:
4090 1389 315 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 2339 2702 3044 4210 1353
Size: 986 363 342 1166 3423
Fragments arranged by size:
3423 1166 986 363 342
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 2938 4867 5241 5387 387
Size: 1064 138 660 689 1929 374 146 1280
Fragments arranged by size:
1929 1280 1064 689 660 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2663 2750 2834 3307 4343 4940 5727
Size: 393 1031 87 84 473 1036 597 787
Cuts at: 5727 5995 1239
Size: 268 1524
Fragments arranged by size:
1524 1036 1031 787 597 473 393 268
87 84
EcoNI CCTnn'n_nnAGG
Cuts at: 3455 3455
Size: 6280
EcoO109I rG'GnC_Cy
Cuts at: 2195 2941 2993 3421 2195
Size: 746 52 428 5054
Fragments arranged by size:
5054 746 428 52
EcoRI G'AATT_C
Cuts at: 3589 3967 3589
Size: 378 5902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3223 3514 3223
Size: 291 5989
FspI TGC'GCA
Cuts at: 3455 4786 3455
Size: 1331 4949
HaeII r_GCGC'y
Cuts at: 1678 2094 2267 2323 2436 2618 3489 5427 1678
Size: 416 173 56 113 182 871 1938 2531
Fragments arranged by size:
2531 1938 871 416 182 173 113 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4921 4953 4995 5027 1373
Size: 32 135 32 3349 32 42 32 2626
Fragments arranged by size:
3349 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3058 3196 3394 3624 3870 669
Size: 988 281 1120 138 198 230 246 3079
Fragments arranged by size:
3079 1120 988 281 246 230 198 138
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6280
HpaI GTT'AAC
Cuts at: 3870 3870
Size: 6280
KasI G'GCGC_C
Cuts at: 3485 3485
Size: 6280
KpnI G_GTAC'C
Cuts at: 3605 3605
Size: 6280
MfeI C'AATT_G
Cuts at: 3879 3879
Size: 6280
MluI A'CGCG_T
Cuts at: 6156 6156
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5394 5578 1305
Size: 4089 184 2007
Fragments arranged by size:
4089 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2468 2717 10
Size: 54 1425 343 636 249 3573
Fragments arranged by size:
3573 1425 636 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2801 3014 3209 3539 4238 4597 4756
Size: 648 1635 213 195 330 699 359 159
Cuts at: 4756 518
Size: 2042
Fragments arranged by size:
2042 1635 699 648 359 330 213 195
159
MspA1I CmG'CkG
Cuts at: 1606 2219 2510 2713 2768 2868 2890 2899 3096
Size: 613 291 203 55 100 22 9 197
Cuts at: 3096 3165 3327 3696 4373 5521 5766 1606
Size: 69 162 369 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 613 369 291 245 203
197 162 100 69 55 22 9
NarI GG'CG_CC
Cuts at: 3486 3486
Size: 6280
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4789
Fragments arranged by size:
4789 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6280
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6280
NotI GC'GGCC_GC
Cuts at: 2127 3727 2127
Size: 1600 4680
NspI r_CATG'y
Cuts at: 1132 1229 3638 5183 1132
Size: 97 2409 1545 2229
Fragments arranged by size:
2409 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2168 2944 1348
Size: 820 776 4684
Fragments arranged by size:
4684 820 776
PciI A'CATG_T
Cuts at: 5179 5179
Size: 6280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 3142 1108
Size: 1524 510 4246
Fragments arranged by size:
4246 1524 510
PfoI T'CCnGG_A
Cuts at: 2811 2811
Size: 6280
PmeI GTTT'AAAC
Cuts at: 3584 3584
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4349 4381 5862 5894 4349
Size: 32 1481 32 4735
Fragments arranged by size:
4735 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2993 2993
Size: 6280
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6280
PsiI TTA'TAA
Cuts at: 3850 3850
Size: 6280
PspOMI G'GGCC_C
Cuts at: 2084 2194 2248 3245 3421 2084
Size: 110 54 997 176 4943
Fragments arranged by size:
4943 997 176 110 54
PspXI vC'TCGA_Gb
Cuts at: 2618 2618
Size: 6280
PstI C_TGCA'G
Cuts at: 830 2271 2755 2904 2992 3632 830
Size: 1441 484 149 88 640 3478
Fragments arranged by size:
3478 1441 640 484 149 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6280
PvuII CAG'CTG
Cuts at: 2713 2899 3327 2713
Size: 186 428 5666
Fragments arranged by size:
5666 428 186
SacII CC_GC'GG
Cuts at: 2220 2769 2220
Size: 549 5731
SalI G'TCGA_C
Cuts at: 1936 3622 1936
Size: 1686 4594
SbfI CC_TGCA'GG
Cuts at: 3632 3632
Size: 6280
ScaI AGT'ACT
Cuts at: 4528 6240 4528
Size: 1712 4568
SfcI C'TryA_G
Cuts at: 826 1045 2267 2751 2900 2988 3160 3628 4763
Size: 219 1222 484 149 88 172 468 1135
Cuts at: 4763 5444 5635 826
Size: 681 191 1471
Fragments arranged by size:
1471 1222 1135 681 484 468 219 191
172 149 88
SfoI GGC'GCC
Cuts at: 3487 3487
Size: 6280
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6280
SmaI CCC'GGG
Cuts at: 1872 2064 2083 2200 2253 3607 1872
Size: 192 19 117 53 1354 4545
Fragments arranged by size:
4545 1354 192 117 53 19
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2528 2618 3416 4384 5285 5547
Size: 197 926 585 90 798 968 901 262
Cuts at: 5547 820
Size: 1553
Fragments arranged by size:
1553 968 926 901 798 585 262 197
90
SnaBI TAC'GTA
Cuts at: 493 4071 493
Size: 3578 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6280
SphI G_CATG'C
Cuts at: 1132 3638 1132
Size: 2506 3774
SrfI GCCC'GGGC
Cuts at: 2064 2064
Size: 6280
SspI AAT'ATT
Cuts at: 5 52 4204 5
Size: 47 4152 2081
Fragments arranged by size:
4152 2081 47
StuI AGG'CCT
Cuts at: 1517 3450 1517
Size: 1933 4347
StyI C'CwwG_G
Cuts at: 513 1065 2004 3719 513
Size: 552 939 1715 3074
Fragments arranged by size:
3074 1715 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4307 4324 4477 4662 5107 325
Size: 1255 2727 17 153 185 445 1498
Fragments arranged by size:
2727 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4526 6238 96
Size: 274 80 33 51 1443 2549 1712 138
Fragments arranged by size:
2549 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2817 2849 6240 6272 2817
Size: 32 3391 32 2825
Fragments arranged by size:
3391 2825 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6280
XbaI T'CTAG_A
Cuts at: 3616 3616
Size: 6280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2725 3070 2725
Size: 345 5935
XhoI C'TCGA_G
Cuts at: 1943 2618 1943
Size: 675 5605
XmaI C'CCGG_G
Cuts at: 1870 2062 2081 2198 2251 3605 1870
Size: 192 19 117 53 1354 4545
Fragments arranged by size:
4545 1354 192 117 53 19
XmnI GAAnn'nnTTC
Cuts at: 4409 4409
Size: 6280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4088 276
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI
RsrII SacI SanDI SapI SexAI SfiI SwaI