Restriction map for
pFN21AE0085
(Circular) (Six-base) MAPSORT of: pFN21AE0085.seq Check: 6741 from: 1 to: 8956
pFN21AE0085 8956 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5776 5776
Size: 8956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6766 278
Size: 53 83 186 936 5230 2468
Fragments arranged by size:
5230 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6299 1937
Size: 4362 4594
Acc65I G'GTAC_C
Cuts at: 6277 6277
Size: 8956
AclI AA'CG_TT
Cuts at: 1563 4716 7083 7456 1563
Size: 3153 2367 373 3063
Fragments arranged by size:
3153 3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 3183 4539 4665 4721 5949 7019 8403
Size: 833 718 1356 126 56 1228 1070 1384
Cuts at: 8403 8671 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1356 1228 1070 833 718 268
126 56
AflII C'TTAA_G
Cuts at: 820 1017 2431 820
Size: 197 1414 7345
Fragments arranged by size:
7345 1414 197
AflIII A'CryG_T
Cuts at: 1203 7855 8832 1203
Size: 6652 977 1327
Fragments arranged by size:
6652 1327 977
AgeI A'CCGG_T
Cuts at: 7787 7787
Size: 8956
AhdI GACnn_n'nnGTC
Cuts at: 7685 7685
Size: 8956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2754 1184
Size: 1570 7386
AlwNI CAG_nnn'CTG
Cuts at: 3827 6699 8271 3827
Size: 2872 1572 4512
Fragments arranged by size:
4512 2872 1572
ApaI G_GGCC'C
Cuts at: 2269 2269
Size: 8956
ApaLI G'TGCA_C
Cuts at: 7013 8169 7013
Size: 1156 7800
ApoI r'AATT_y
Cuts at: 1493 2204 2509 2777 2887 3255 3334 3428 3720
Size: 711 305 268 110 368 79 94 292
Cuts at: 3720 3973 4245 4275 4560 6265 6491 6643 8752
Size: 253 272 30 285 1705 226 152 2109
Cuts at: 8752 1493
Size: 1697
Fragments arranged by size:
2109 1705 1697 711 368 305 292 285
272 268 253 226 152 110 94 79
30
AseI AT'TA_AT
Cuts at: 160 1032 4227 7510 160
Size: 872 3195 3283 1606
Fragments arranged by size:
3283 3195 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8956
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2080 2268 2301 5778 6281 1870
Size: 73 114 23 188 33 3477 503 4545
Fragments arranged by size:
4545 3477 503 188 114 73 33 23
BamHI G'GATC_C
Cuts at: 6286 6653 6286
Size: 367 8589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2130 6277 7732 618
Size: 325 217 627 343 4147 1455 1842
Fragments arranged by size:
4147 1842 1455 627 343 325 217
BanII G_rGCy'C
Cuts at: 1391 2068 2081 2269 5379 6275 1391
Size: 677 13 188 3110 896 4072
Fragments arranged by size:
4072 3110 896 677 188 13
BbeI G_GCGC'C
Cuts at: 2134 2134
Size: 8956
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3645 4685 5367 6093 928
Size: 971 1746 1040 682 726 3791
Fragments arranged by size:
3791 1746 1040 971 726 682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2192 2226 7147 7181 1903
Size: 34 255 34 4921 34 3678
Fragments arranged by size:
4921 3678 255 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6157 6848 8058 6157
Size: 691 1210 7055
Fragments arranged by size:
7055 1210 691
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7567 136
Size: 107 122 71 631 605 5895 1525
Fragments arranged by size:
5895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8951 8951
Size: 8956
BlpI GC'TnA_GC
Cuts at: 6373 6373
Size: 8956
Bme1580I G_kGCm'C
Cuts at: 1790 2269 7017 8173 1790
Size: 479 4748 1156 2573
Fragments arranged by size:
4748 2573 1156 479
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3553 5945 6687
Size: 416 53 83 399 24 2136 2392 742
Cuts at: 6687 7645 442
Size: 958 1753
Fragments arranged by size:
2392 2136 1753 958 742 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4042 5237 5537 5894 7616 1239
Size: 2803 1195 300 357 1722 2579
Fragments arranged by size:
2803 2579 1722 1195 357 300
Bpu10I CC'TnA_GC
Cuts at: 5436 6159 5436
Size: 723 8233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2852 2895 4130 5219 7081 7946 8244 2852
Size: 43 1235 1089 1862 865 298 3564
Fragments arranged by size:
3564 1862 1235 1089 865 298 43
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2337 7619 882
Size: 617 838 5282 2219
Fragments arranged by size:
5282 2219 838 617
BsaAI yAC'GTr
Cuts at: 493 1204 3944 5954 6747 493
Size: 711 2740 2010 793 2702
Fragments arranged by size:
2740 2702 2010 793 711
BsaBI GATnn'nnATC
Cuts at: 3873 8950 3873
Size: 5077 3879
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2131 6763 7145 7780
Size: 53 83 186 936 598 4632 382 635
Cuts at: 7780 275
Size: 1451
Fragments arranged by size:
4632 1451 936 635 598 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7389 7787 8061 8208 8619 7389
Size: 398 274 147 411 7726
Fragments arranged by size:
7726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6237 6267 1178
Size: 30 165 30 4834 30 3867
Fragments arranged by size:
4834 3867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4046 4901 5490 1186
Size: 2860 855 589 4652
Fragments arranged by size:
4652 2860 855 589
BseYI C'CCAG_C
Cuts at: 2339 8159 8655 2339
Size: 5820 496 2640
Fragments arranged by size:
5820 2640 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4890 5810 1163
Size: 573 3154 920 4309
Fragments arranged by size:
4309 3154 920 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6406 7167 8195 1540
Size: 275 186 4405 761 1028 2301
Fragments arranged by size:
4405 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5379 5472 7017 7102 8173 5379
Size: 93 1545 85 1071 6162
Fragments arranged by size:
6162 1545 1071 93 85
BsmI GAATG_Cn'
Cuts at: 2743 5348 5982 6467 6560 2743
Size: 2605 634 485 93 5139
Fragments arranged by size:
5139 2605 634 485 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2081 2269 5379 5472 6275 7017
Size: 399 278 13 188 3110 93 803 742
Cuts at: 7017 7102 8173 1391
Size: 85 1071 2174
Fragments arranged by size:
3110 2174 1071 803 742 399 278 188
93 85 13
BspEI T'CCGG_A
Cuts at: 8619 8619
Size: 8956
BspHI T'CATG_A
Cuts at: 6845 8575 6845
Size: 1730 7226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5776 6311 844
Size: 4932 535 3489
Fragments arranged by size:
4932 3489 535
BsrBI CCG'CTC
Cuts at: 1394 1823 6843 1394
Size: 429 5020 3507
Fragments arranged by size:
5020 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5061 5724 7451 7625 65
Size: 1183 3813 663 1727 174 1396
Fragments arranged by size:
3813 1727 1396 1183 663 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7600 7787 1700
Size: 110 5790 187 2869
Fragments arranged by size:
5790 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8956
BssHII G'CGCG_C
Cuts at: 1925 2085 2313 1925
Size: 160 228 8568
Fragments arranged by size:
8568 228 160
BssSI C'ACGA_G
Cuts at: 7016 8028 7016
Size: 1012 7944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2423 3654 4163 794
Size: 1629 1231 509 5587
Fragments arranged by size:
5587 1629 1231 509
BstBI TT'CG_AA
Cuts at: 2202 7760 2202
Size: 5558 3398
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8956
BstXI CCAn_nnnn'nTGG
Cuts at: 4066 5755 4066
Size: 1689 7267
BstYI r'GATC_y
Cuts at: 1972 5320 6286 6653 7038 7055 8507 8593 8605
Size: 3348 966 367 385 17 1452 86 12
Cuts at: 8605 8951 1972
Size: 346 1977
Fragments arranged by size:
3348 1977 1452 966 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2192 513
Size: 552 939 188 7277
Fragments arranged by size:
7277 939 552 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8217
Fragments arranged by size:
8217 683 56
BtsI GCAGTG_nn'
Cuts at: 2167 3333 4953 6472 7266 7286 2167
Size: 1166 1620 1519 794 20 3837
Fragments arranged by size:
3837 1620 1519 1166 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8921
DraI TTT'AAA
Cuts at: 3592 4357 4494 6260 3592
Size: 765 137 1766 6288
Fragments arranged by size:
6288 1766 765 137
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7963 809
Size: 507 6647 1802
Fragments arranged by size:
6647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6403 7292 8921
Size: 54 1425 50 275 18 4573 889 1629
Cuts at: 8921 8
Size: 43
Fragments arranged by size:
4573 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6403 1537
Size: 275 4591 4090
Fragments arranged by size:
4591 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 2475 3090 4641 4740 4989 6886 1353
Size: 1041 81 615 1551 99 249 1897 3423
Fragments arranged by size:
3423 1897 1551 1041 615 249 99 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7543 7917 8063 387
Size: 1064 138 5954 374 146 1280
Fragments arranged by size:
5954 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5377 5377
Size: 8956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 3183 4042 4539 4665 4721 5237
Size: 393 833 718 859 497 126 56 516
Cuts at: 5237 5537 5894 5949 7019 7616 8403 8671 1239
Size: 300 357 55 1070 597 787 268 1524
Fragments arranged by size:
1524 1070 859 833 787 718 597 516
497 393 357 300 268 126 56 55
EcoO109I rG'GnC_Cy
Cuts at: 2265 2265
Size: 8956
EcoRI G'AATT_C
Cuts at: 2204 6265 6643 2204
Size: 4061 378 4517
Fragments arranged by size:
4517 4061 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3043 5615 6251 3043
Size: 2572 636 5748
Fragments arranged by size:
5748 2572 636
FspI TGC'GCA
Cuts at: 7462 7462
Size: 8956
HaeII r_GCGC'y
Cuts at: 1678 2134 8103 1678
Size: 456 5969 2531
Fragments arranged by size:
5969 2531 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3736 3768 4195 4227 4669
Size: 32 135 32 2164 32 427 32 442
Cuts at: 4669 4701 5071 5103 5548 5580 6037 6069 7597
Size: 32 370 32 445 32 457 32 1528
Cuts at: 7597 7629 7671 7703 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2164 1528 457 445 442 427 370
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3503 3991 5648 6300 6546 669
Size: 988 281 1565 488 1657 652 246 3079
Fragments arranged by size:
3079 1657 1565 988 652 488 281 246
HindIII A'AGCT_T
Cuts at: 748 3108 3698 3728 4047 4488 6027 748
Size: 2360 590 30 319 441 1539 3677
Fragments arranged by size:
3677 2360 1539 590 441 319 30
HpaI GTT'AAC
Cuts at: 3991 6546 3991
Size: 2555 6401
KasI G'GCGC_C
Cuts at: 2130 2130
Size: 8956
KpnI G_GTAC'C
Cuts at: 6281 6281
Size: 8956
MfeI C'AATT_G
Cuts at: 4595 6555 4595
Size: 1960 6996
MluI A'CGCG_T
Cuts at: 8832 8832
Size: 8956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3139 8070 8254 1305
Size: 1834 4931 184 2007
Fragments arranged by size:
4931 2007 1834 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7134
Fragments arranged by size:
7134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3326 6914 7273 7432 518
Size: 648 2160 3588 359 159 2042
Fragments arranged by size:
3588 2160 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2194 4021 4120 4865 4904 5330 6372 7049
Size: 588 1827 99 745 39 426 1042 677
Cuts at: 7049 8197 8442 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1827 1148 1042 745 677 588 426
245 99 39
NarI GG'CG_CC
Cuts at: 2131 2131
Size: 8956
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7465
Fragments arranged by size:
7465 939 552
NdeI CA'TA_TG
Cuts at: 387 6036 387
Size: 5649 3307
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8956
NotI GC'GGCC_GC
Cuts at: 6403 6403
Size: 8956
NspI r_CATG'y
Cuts at: 1132 1229 6314 7859 1132
Size: 97 5085 1545 2229
Fragments arranged by size:
5085 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8956
PciI A'CATG_T
Cuts at: 7855 7855
Size: 8956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2962 1108
Size: 1854 7102
PfoI T'CCnGG_A
Cuts at: 5253 5253
Size: 8956
PmeI GTTT'AAAC
Cuts at: 6260 6260
Size: 8956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7025 7057 8538 8570 7025
Size: 32 1481 32 7411
Fragments arranged by size:
7411 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8956
PsiI TTA'TAA
Cuts at: 2885 5618 6526 2885
Size: 2733 908 5315
Fragments arranged by size:
5315 2733 908
PspOMI G'GGCC_C
Cuts at: 2265 2265
Size: 8956
PspXI vC'TCGA_Gb
Cuts at: 2301 2301
Size: 8956
PstI C_TGCA'G
Cuts at: 830 6308 830
Size: 5478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8956
PvuII CAG'CTG
Cuts at: 4021 4120 4865 4904 5330 4021
Size: 99 745 39 426 7647
Fragments arranged by size:
7647 745 426 99 39
SacI G_AGCT'C
Cuts at: 5379 5379
Size: 8956
SacII CC_GC'GG
Cuts at: 2195 2195
Size: 8956
SalI G'TCGA_C
Cuts at: 1936 6298 1936
Size: 4362 4594
SapI GCTCTTCn'nnn_
Cuts at: 3090 4989 3090
Size: 1899 7057
SbfI CC_TGCA'GG
Cuts at: 6308 6308
Size: 8956
ScaI AGT'ACT
Cuts at: 5155 5196 5239 7204 8916 5155
Size: 41 43 1965 1712 5195
Fragments arranged by size:
5195 1965 1712 43 41
SfcI C'TryA_G
Cuts at: 826 1045 4724 5273 6304 7439 8120 8311 826
Size: 219 3679 549 1031 1135 681 191 1471
Fragments arranged by size:
3679 1471 1135 1031 681 549 219 191
SfoI GGC'GCC
Cuts at: 2132 2132
Size: 8956
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8956
SmaI CCC'GGG
Cuts at: 1872 2059 2270 5780 6283 1872
Size: 187 211 3510 503 4545
Fragments arranged by size:
4545 3510 503 211 187
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2301 2431 2831 2910 4109 5198
Size: 197 926 358 130 400 79 1199 1089
Cuts at: 5198 7060 7961 8223 820
Size: 1862 901 262 1553
Fragments arranged by size:
1862 1553 1199 1089 926 901 400 358
262 197 130 79
SnaBI TAC'GTA
Cuts at: 493 3944 6747 493
Size: 3451 2803 2702
Fragments arranged by size:
3451 2803 2702
SpeI A'CTAG_T
Cuts at: 152 4007 152
Size: 3855 5101
SphI G_CATG'C
Cuts at: 1132 6314 1132
Size: 5182 3774
SspI AAT'ATT
Cuts at: 5 52 3174 4225 6880 5
Size: 47 3122 1051 2655 2081
Fragments arranged by size:
3122 2655 2081 1051 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8956
StyI C'CwwG_G
Cuts at: 513 1065 2004 6395 513
Size: 552 939 4391 3074
Fragments arranged by size:
4391 3074 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 6983 7000 7153 7338 7783 325
Size: 1255 762 4641 17 153 185 445 1498
Fragments arranged by size:
4641 1498 1255 762 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4986 5153 5194
Size: 274 80 33 51 1443 3009 167 41
Cuts at: 5194 5237 5269 5721 5736 7202 8914 96
Size: 43 32 452 15 1466 1712 138
Fragments arranged by size:
3009 1712 1466 1443 452 274 167 138
80 51 43 41 33 32 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6237 6269 8916 8948 6237
Size: 32 2647 32 6245
Fragments arranged by size:
6245 2647 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4375 1316
Size: 3059 5897
XbaI T'CTAG_A
Cuts at: 3365 4213 6292 3365
Size: 848 2079 6029
Fragments arranged by size:
6029 2079 848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 2347
Size: 8956
XhoI C'TCGA_G
Cuts at: 1943 2301 1943
Size: 358 8598
XmaI C'CCGG_G
Cuts at: 1870 2057 2268 5778 6281 1870
Size: 187 211 3510 503 4545
Fragments arranged by size:
4545 3510 503 211 187
XmnI GAAnn'nnTTC
Cuts at: 2787 3153 5598 7085 2787
Size: 366 2445 1487 4658
Fragments arranged by size:
4658 2445 1487 366
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6764 276
Size: 53 83 186 936 5230 2468
Fragments arranged by size:
5230 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PsrI RsrII SanDI SexAI SfiI SrfI SwaI