Restriction map for
pFN21AE0084
(Circular) (Six-base) MAPSORT of: pFN21AE0084.seq Check: 9461 from: 1 to: 7321
pFN21AE0084 7321 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3946 3946
Size: 7321
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2862 5131 278
Size: 53 83 186 936 1326 2269 2468
Fragments arranged by size:
2468 2269 1326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2841 4664 1937
Size: 904 1823 4594
Fragments arranged by size:
4594 1823 904
Acc65I G'GTAC_C
Cuts at: 4497 4642 4497
Size: 145 7176
AclI AA'CG_TT
Cuts at: 1563 5448 5821 1563
Size: 3885 373 3063
Fragments arranged by size:
3885 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 3220 3486 3569 3680 4026 4408 5384
Size: 965 623 266 83 111 346 382 976
Cuts at: 5384 6768 7036 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 976 965 623 382 346 268
266 111 83
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7124
AflIII A'CryG_T
Cuts at: 1203 2382 6220 7197 1203
Size: 1179 3838 977 1327
Fragments arranged by size:
3838 1327 1179 977
AgeI A'CCGG_T
Cuts at: 6152 6152
Size: 7321
AhdI GACnn_n'nnGTC
Cuts at: 6050 6050
Size: 7321
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3672 1184
Size: 2488 4833
AlwNI CAG_nnn'CTG
Cuts at: 2707 3131 5064 6636 2707
Size: 424 1933 1572 3392
Fragments arranged by size:
3392 1933 1572 424
ApaI G_GGCC'C
Cuts at: 4310 4310
Size: 7321
ApaLI G'TGCA_C
Cuts at: 5378 6534 5378
Size: 1156 6165
ApoI r'AATT_y
Cuts at: 1493 3573 3602 4630 4856 5008 7117 1493
Size: 2080 29 1028 226 152 2109 1697
Fragments arranged by size:
2109 2080 1697 1028 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 5875 160
Size: 872 4843 1606
Fragments arranged by size:
4843 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7321
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 2458 4167 4272 4646 1870
Size: 73 231 284 1709 105 374 4545
Fragments arranged by size:
4545 1709 374 284 231 105 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2791 2824 2791
Size: 33 7288
BamHI G'GATC_C
Cuts at: 4651 5018 4651
Size: 367 6954
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2140 2738 3989 4497 4642
Size: 325 217 627 353 598 1251 508 145
Cuts at: 4642 6097 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1251 627 598 508 353 325
217 145
BanII G_rGCy'C
Cuts at: 1391 2013 2265 2759 3138 4310 4640 1391
Size: 622 252 494 379 1172 330 4072
Fragments arranged by size:
4072 1172 622 494 379 330 252
BbeI G_GCGC'C
Cuts at: 2144 2144
Size: 7321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3504 3537 3852 928
Size: 971 1605 33 315 4397
Fragments arranged by size:
4397 1605 971 315 33
BbvCI CC'TCA_GC
Cuts at: 4213 4213
Size: 7321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4263 4297 5512 5546 1903
Size: 34 2326 34 1215 34 3678
Fragments arranged by size:
3678 2326 1215 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5213 6423 5213
Size: 1210 6111
BclI T'GATC_A
Cuts at: 1548 2937 1548
Size: 1389 5932
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5932 136
Size: 107 122 71 631 605 4260 1525
Fragments arranged by size:
4260 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7316 7316
Size: 7321
BlpI GC'TnA_GC
Cuts at: 2282 2758 3132 4738 2282
Size: 476 374 1606 4865
Fragments arranged by size:
4865 1606 476 374
Bme1580I G_kGCm'C
Cuts at: 1790 2773 3992 4310 5382 6538 1790
Size: 983 1219 318 1072 1156 2573
Fragments arranged by size:
2573 1219 1156 1072 983 318
BmgBI CAC'GTC
Cuts at: 1318 2152 1318
Size: 834 6487
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2285 4173 5052
Size: 416 53 83 399 24 868 1888 879
Cuts at: 5052 6010 442
Size: 958 1753
Fragments arranged by size:
1888 1753 958 879 868 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7321
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 7321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2369 2501 3172 3272 4424 5981 1239
Size: 992 138 132 671 100 1152 1557 2579
Fragments arranged by size:
2579 1557 1152 992 671 138 132 100
Bpu10I CC'TnA_GC
Cuts at: 4213 4213
Size: 7321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1997 3059 5446 6311 6609 1997
Size: 1062 2387 865 298 2709
Fragments arranged by size:
2709 2387 1062 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2347 2900 3284 4416 5984 882
Size: 617 848 553 384 1132 1568 2219
Fragments arranged by size:
2219 1568 1132 848 617 553 384
BsaAI yAC'GTr
Cuts at: 493 1204 5112 493
Size: 711 3908 2702
Fragments arranged by size:
3908 2702 711
BsaBI GATnn'nnATC
Cuts at: 7315 7315
Size: 7321
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 2343 2859 3024
Size: 53 83 186 936 608 202 516 165
Cuts at: 3024 5128 5510 6145 275
Size: 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 608 516 382 202
186 165 83 53
BsaWI w'CCGG_w
Cuts at: 2727 5754 6152 6426 6573 6984 2727
Size: 3027 398 274 147 411 3064
Fragments arranged by size:
3064 3027 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4310 4340 4481 4511 1178
Size: 30 165 30 2907 30 141 30 3988
Fragments arranged by size:
3988 2907 165 141 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2900 2912 3985 4194 4412 1186
Size: 949 765 12 1073 209 218 4095
Fragments arranged by size:
4095 1073 949 765 218 209 12
BseYI C'CCAG_C
Cuts at: 2086 2364 3274 3789 4544 6524 7020 2086
Size: 278 910 515 755 1980 496 2387
Fragments arranged by size:
2387 1980 910 755 515 496 278
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3101 3980 1163
Size: 573 1365 879 4504
Fragments arranged by size:
4504 1365 879 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 2224 4771 5532 6560 1540
Size: 275 186 111 112 2547 761 1028 2301
Fragments arranged by size:
2547 2301 1028 761 275 186 112 111
BsiHKAI G_wGCw'C
Cuts at: 3138 5382 5467 6538 3138
Size: 2244 85 1071 3921
Fragments arranged by size:
3921 2244 1071 85
BsmI GAATG_Cn'
Cuts at: 3773 4832 4925 3773
Size: 1059 93 6169
Fragments arranged by size:
6169 1059 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3031 3226 3593 4333 4508 3031
Size: 195 367 740 175 5844
Fragments arranged by size:
5844 740 367 195 175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2265 2759 2773 3138 3992 4310
Size: 399 223 252 494 14 365 854 318
Cuts at: 4310 4640 5382 5467 6538 1391
Size: 330 742 85 1071 2174
Fragments arranged by size:
2174 1071 854 742 494 399 365 330
318 252 223 85 14
BspEI T'CCGG_A
Cuts at: 6984 6984
Size: 7321
BspHI T'CATG_A
Cuts at: 5210 6940 5210
Size: 1730 5591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3946 3952 4170 4308 4676 844
Size: 3102 6 218 138 368 3489
Fragments arranged by size:
3489 3102 368 218 138 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 5208 1394
Size: 429 302 3083 3507
Fragments arranged by size:
3507 3083 429 302
BsrDI GCAATG_nn'
Cuts at: 65 1248 2668 5816 5990 65
Size: 1183 1420 3148 174 1396
Fragments arranged by size:
3148 1420 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 2316 5965 6152 1700
Size: 110 301 205 3649 187 2869
Fragments arranged by size:
3649 2869 301 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7321
BssHII G'CGCG_C
Cuts at: 1925 2018 1925
Size: 93 7228
BssSI C'ACGA_G
Cuts at: 3309 3920 4394 5381 6393 3309
Size: 611 474 987 1012 4237
Fragments arranged by size:
4237 1012 987 611 474
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2707 794
Size: 1913 5408
BstBI TT'CG_AA
Cuts at: 6125 6125
Size: 7321
BstEII G'GTnAC_C
Cuts at: 1184 3482 4108 1184
Size: 2298 626 4397
Fragments arranged by size:
4397 2298 626
BstYI r'GATC_y
Cuts at: 1972 2526 2703 4651 5018 5403 5420 6872 6958
Size: 554 177 1948 367 385 17 1452 86
Cuts at: 6958 6970 7316 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1948 1452 554 385 367 346 177
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2357 3489 4239 513
Size: 552 939 353 1132 750 3595
Fragments arranged by size:
3595 1132 939 750 552 353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6582
Fragments arranged by size:
6582 683 56
BtsI GCAGTG_nn'
Cuts at: 4837 5631 5651 4837
Size: 794 20 6507
Fragments arranged by size:
6507 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7321
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4209 4244 529
Size: 35 3645 35 3606
Fragments arranged by size:
3645 3606 35 35
DraI TTT'AAA
Cuts at: 4625 4625
Size: 7321
DraIII CAC_nnn'GTG
Cuts at: 4108 4108
Size: 7321
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6328 809
Size: 507 5012 1802
Fragments arranged by size:
5012 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2221 2268
Size: 54 1425 50 275 18 279 112 47
Cuts at: 2268 2670 2946 4768 5657 7286 8
Size: 402 276 1822 889 1629 43
Fragments arranged by size:
1822 1629 1425 889 402 279 276 275
112 54 50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2109 2221 4768 1537
Size: 275 297 112 2547 4090
Fragments arranged by size:
4090 2547 297 275 112
EarI CTCTTCn'nnn_
Cuts at: 1353 4266 5251 1353
Size: 2913 985 3423
Fragments arranged by size:
3423 2913 985
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 3509 5908 6282 6428 387
Size: 1064 138 490 1430 2399 374 146 1280
Fragments arranged by size:
2399 1430 1280 1064 490 374 146 138
EcoICRI GAG'CTC
Cuts at: 3136 3136
Size: 7321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2369 2501 2597 3172 3220 3272
Size: 393 599 138 132 96 575 48 52
Cuts at: 3272 3486 3569 3680 4026 4408 4424 5384 5981
Size: 214 83 111 346 382 16 960 597
Cuts at: 5981 6768 7036 1239
Size: 787 268 1524
Fragments arranged by size:
1524 960 787 599 597 575 393 382
346 268 214 138 132 111 96 83
52 48 16
EcoNI CCTnn'n_nnAGG
Cuts at: 4505 4505
Size: 7321
EcoO109I rG'GnC_Cy
Cuts at: 2199 2244 2932 4076 4175 4306 4307 2199
Size: 45 688 1144 99 131 1 5213
Fragments arranged by size:
5213 1144 688 131 99 45 1
EcoRI G'AATT_C
Cuts at: 3573 4630 5008 3573
Size: 1057 378 5886
Fragments arranged by size:
5886 1057 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3931 3931
Size: 7321
FspI TGC'GCA
Cuts at: 2711 5827 2711
Size: 3116 4205
HaeII r_GCGC'y
Cuts at: 1678 2144 2174 2372 6468 1678
Size: 466 30 198 4096 2531
Fragments arranged by size:
4096 2531 466 198 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3245 3277 4310 4332 4342
Size: 32 135 32 1673 32 1033 22 10
Cuts at: 4342 4364 5962 5994 6036 6068 1373
Size: 22 1598 32 42 32 2626
Fragments arranged by size:
2626 1673 1598 1033 135 42 32 32
32 32 32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 4390 4665 4911 669
Size: 988 281 2452 275 246 3079
Fragments arranged by size:
3079 2452 988 281 275 246
HindIII A'AGCT_T
Cuts at: 748 2747 748
Size: 1999 5322
HpaI GTT'AAC
Cuts at: 4911 4911
Size: 7321
KasI G'GCGC_C
Cuts at: 2140 2140
Size: 7321
KpnI G_GTAC'C
Cuts at: 4501 4646 4501
Size: 145 7176
MfeI C'AATT_G
Cuts at: 4920 4920
Size: 7321
MluI A'CGCG_T
Cuts at: 2382 7197 2382
Size: 4815 2506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2586 6435 6619 1305
Size: 1281 3849 184 2007
Fragments arranged by size:
3849 2007 1281 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 2672 2948 10
Size: 54 1425 343 438 402 276 4383
Fragments arranged by size:
4383 1425 438 402 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 5279 5638 5797 518
Size: 648 1449 2664 359 159 2042
Fragments arranged by size:
2664 2042 1449 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2239 2281 2359 3047 3128 3250 4004 4548
Size: 633 42 78 688 81 122 754 544
Cuts at: 4548 4737 5414 6562 6807 1606
Size: 189 677 1148 245 2120
Fragments arranged by size:
2120 1148 754 688 677 633 544 245
189 122 81 78 42
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 7321
NarI GG'CG_CC
Cuts at: 2141 2141
Size: 7321
NcoI C'CATG_G
Cuts at: 513 1065 2004 3489 513
Size: 552 939 1485 4345
Fragments arranged by size:
4345 1485 939 552
NdeI CA'TA_TG
Cuts at: 387 3814 387
Size: 3427 3894
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 7321
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7321
NotI GC'GGCC_GC
Cuts at: 2221 4768 2221
Size: 2547 4774
NspI r_CATG'y
Cuts at: 1132 1229 2605 2716 3676 3937 4679 6224 1132
Size: 97 1376 111 960 261 742 1545 2229
Fragments arranged by size:
2229 1545 1376 960 742 261 111 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7321
PciI A'CATG_T
Cuts at: 6220 6220
Size: 7321
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2386 2982 3274 1108
Size: 1278 596 292 5155
Fragments arranged by size:
5155 1278 596 292
PfoI T'CCnGG_A
Cuts at: 3680 3680
Size: 7321
PmeI GTTT'AAAC
Cuts at: 4625 4625
Size: 7321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5390 5422 6903 6935 5390
Size: 32 1481 32 5776
Fragments arranged by size:
5776 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2199 2244 4175 2199
Size: 45 1931 5345
Fragments arranged by size:
5345 1931 45
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7321
PsiI TTA'TAA
Cuts at: 4891 4891
Size: 7321
PspOMI G'GGCC_C
Cuts at: 4306 4306
Size: 7321
PstI C_TGCA'G
Cuts at: 830 2703 4305 4673 830
Size: 1873 1602 368 3478
Fragments arranged by size:
3478 1873 1602 368
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7321
PvuII CAG'CTG
Cuts at: 3128 3250 4004 3128
Size: 122 754 6445
Fragments arranged by size:
6445 754 122
RsrII CG'GwC_CG
Cuts at: 4318 4318
Size: 7321
SacI G_AGCT'C
Cuts at: 3138 3138
Size: 7321
SacII CC_GC'GG
Cuts at: 2360 2360
Size: 7321
SalI G'TCGA_C
Cuts at: 1936 4663 1936
Size: 2727 4594
SanDI GG'GwC_CC
Cuts at: 2199 4175 2199
Size: 1976 5345
SbfI CC_TGCA'GG
Cuts at: 4305 4673 4305
Size: 368 6953
ScaI AGT'ACT
Cuts at: 5569 7281 5569
Size: 1712 5609
SexAI A'CCwGG_T
Cuts at: 2844 3186 4104 2844
Size: 342 918 6061
Fragments arranged by size:
6061 918 342
SfcI C'TryA_G
Cuts at: 826 1045 2699 4093 4301 4669 5804 6485 6676
Size: 219 1654 1394 208 368 1135 681 191
Cuts at: 6676 826
Size: 1471
Fragments arranged by size:
1654 1471 1394 1135 681 368 219 208
191
SfoI GGC'GCC
Cuts at: 2142 2142
Size: 7321
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7321
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7321
SmaI CCC'GGG
Cuts at: 1872 4648 1872
Size: 2776 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 3074 5425 6326 6588 820
Size: 197 926 69 1062 2351 901 262 1553
Fragments arranged by size:
2351 1553 1062 926 901 262 197 69
SnaBI TAC'GTA
Cuts at: 493 5112 493
Size: 4619 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7321
SphI G_CATG'C
Cuts at: 1132 2716 4679 1132
Size: 1584 1963 3774
Fragments arranged by size:
3774 1963 1584
SspI AAT'ATT
Cuts at: 5 52 5245 5
Size: 47 5193 2081
Fragments arranged by size:
5193 2081 47
StuI AGG'CCT
Cuts at: 1517 3622 1517
Size: 2105 5216
StyI C'CwwG_G
Cuts at: 513 1065 2004 2166 2656 3489 3984 4760 513
Size: 552 939 162 490 833 495 776 3074
Fragments arranged by size:
3074 939 833 776 552 495 490 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2989 4335 4375 5348 5365 5518 5703
Size: 1255 1409 1346 40 973 17 153 185
Cuts at: 5703 6148 325
Size: 445 1498
Fragments arranged by size:
1498 1409 1346 1255 973 445 185 153
40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5567 7279 96
Size: 274 80 33 51 1443 3590 1712 138
Fragments arranged by size:
3590 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7281 7313 7281
Size: 32 7289
Tth111I GACn'n_nGTC
Cuts at: 1316 4121 1316
Size: 2805 4516
XbaI T'CTAG_A
Cuts at: 4657 4657
Size: 7321
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3271 3271
Size: 7321
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7321
XmaI C'CCGG_G
Cuts at: 1870 4646 1870
Size: 2776 4545
XmnI GAAnn'nnTTC
Cuts at: 3518 5450 3518
Size: 1932 5389
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2860 5129 276
Size: 53 83 186 936 1326 2269 2468
Fragments arranged by size:
2468 2269 1326 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BstXI BstZ17I
Bsu36I EcoRV FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI SapI SfiI SrfI SwaI