Restriction map for
pFN21AE0082
(Circular) (Six-base) MAPSORT of: pFN21AE0082.seq Check: 9975 from: 1 to: 6691
pFN21AE0082 6691 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2106 2106
Size: 6691
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4501 278
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4034 1937
Size: 2097 4594
Acc65I G'GTAC_C
Cuts at: 4012 4012
Size: 6691
AclI AA'CG_TT
Cuts at: 1563 4818 5191 1563
Size: 3255 373 3063
Fragments arranged by size:
3255 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2303 2752 2858 3479 4754 6138 6406 1632
Size: 671 449 106 621 1275 1384 268 1917
Fragments arranged by size:
1917 1384 1275 671 621 449 268 106
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6494
AflIII A'CryG_T
Cuts at: 1203 2267 5590 6567 1203
Size: 1064 3323 977 1327
Fragments arranged by size:
3323 1327 1064 977
AgeI A'CCGG_T
Cuts at: 5522 5522
Size: 6691
AhdI GACnn_n'nnGTC
Cuts at: 5420 5420
Size: 6691
AleI CACnn'nnGTG
Cuts at: 1166 3567 1166
Size: 2401 4290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6691
AlwNI CAG_nnn'CTG
Cuts at: 4434 6006 4434
Size: 1572 5119
ApaLI G'TGCA_C
Cuts at: 3670 4748 5904 3670
Size: 1078 1156 4457
Fragments arranged by size:
4457 1156 1078
ApoI r'AATT_y
Cuts at: 1493 2073 2802 2970 3098 3182 3350 3434 3558
Size: 580 729 168 128 84 168 84 124
Cuts at: 3558 3602 3700 3770 3894 3952 4000 4226 4378
Size: 44 98 70 124 58 48 226 152
Cuts at: 4378 6487 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 729 580 226 168 168 152
128 124 124 98 84 84 70 58
48 44
AseI AT'TA_AT
Cuts at: 160 1032 5245 160
Size: 872 4213 1606
Fragments arranged by size:
4213 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
AvaI C'yCGr_G
Cuts at: 1870 1943 4016 1870
Size: 73 2073 4545
Fragments arranged by size:
4545 2073 73
BamHI G'GATC_C
Cuts at: 4021 4388 4021
Size: 367 6324
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2176 3717 4012 5467 618
Size: 325 217 627 389 1541 295 1455 1842
Fragments arranged by size:
1842 1541 1455 627 389 325 295 217
BanII G_rGCy'C
Cuts at: 1391 4010 1391
Size: 2619 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5720
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4882 4916 1903
Size: 34 2945 34 3678
Fragments arranged by size:
3678 2945 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4583 5793 4583
Size: 1210 5481
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6691
BfrBI ATG'CAT
Cuts at: 2451 2451
Size: 6691
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5302 136
Size: 107 122 71 631 605 3630 1525
Fragments arranged by size:
3630 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2485 6686 2485
Size: 4201 2490
BlpI GC'TnA_GC
Cuts at: 2304 4108 2304
Size: 1804 4887
Bme1580I G_kGCm'C
Cuts at: 1790 3674 4752 5908 1790
Size: 1884 1078 1156 2573
Fragments arranged by size:
2573 1884 1156 1078
BmgBI CAC'GTC
Cuts at: 1318 2041 1318
Size: 723 5968
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4422 5380 442
Size: 416 53 83 399 24 3005 958 1753
Fragments arranged by size:
3005 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6691
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2393 2393
Size: 6691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2748 2832 3000 3084 3168 3252 3336
Size: 899 610 84 168 84 84 84 84
Cuts at: 3336 3420 3504 3840 3856 3924 5351 1239
Size: 84 84 336 16 68 1427 2579
Fragments arranged by size:
2579 1427 899 610 336 168 84 84
84 84 84 84 84 68 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4816 5681 5979 4816
Size: 865 298 5528
Fragments arranged by size:
5528 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3904 5354 882
Size: 617 2405 1450 2219
Fragments arranged by size:
2405 2219 1450 617
BsaAI yAC'GTr
Cuts at: 493 1204 4482 493
Size: 711 3278 2702
Fragments arranged by size:
3278 2702 711
BsaBI GATnn'nnATC
Cuts at: 2484 3549 6685 2484
Size: 1065 3136 2490
Fragments arranged by size:
3136 2490 1065
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4498 4880 5515 275
Size: 53 83 186 936 2965 382 635 1451
Fragments arranged by size:
2965 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5124 5522 5796 5943 6354 5124
Size: 398 274 147 411 5461
Fragments arranged by size:
5461 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6466
Fragments arranged by size:
6466 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2186 1186
Size: 892 108 5691
Fragments arranged by size:
5691 892 108
BseYI C'CCAG_C
Cuts at: 2253 5894 6390 2253
Size: 3641 496 2554
Fragments arranged by size:
3641 2554 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 2333 2563 2683 2851 3100 3859
Size: 573 348 249 230 120 168 249 759
Cuts at: 3859 3943 1163
Size: 84 3911
Fragments arranged by size:
3911 759 573 348 249 249 230 168
120 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4141 4902 5930 1540
Size: 275 186 2140 761 1028 2301
Fragments arranged by size:
2301 2140 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2628 3674 4752 4837 5908 2628
Size: 1046 1078 85 1071 3411
Fragments arranged by size:
3411 1078 1071 1046 85
BsmI GAATG_Cn'
Cuts at: 3170 3338 3422 3506 4202 4295 3170
Size: 168 84 84 696 93 5566
Fragments arranged by size:
5566 696 168 93 84 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2628 3674 4010 4752 4837 5908 1391
Size: 399 838 1046 336 742 85 1071 2174
Fragments arranged by size:
2174 1071 1046 838 742 399 336 85
BspEI T'CCGG_A
Cuts at: 6354 6354
Size: 6691
BspHI T'CATG_A
Cuts at: 3802 4580 6310 3802
Size: 778 1730 4183
Fragments arranged by size:
4183 1730 778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2106 2355 4046 844
Size: 1262 249 1691 3489
Fragments arranged by size:
3489 1691 1262 249
BsrBI CCG'CTC
Cuts at: 1394 1823 4578 1394
Size: 429 2755 3507
Fragments arranged by size:
3507 2755 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2195 3007 5186 5360 65
Size: 1183 947 812 2179 174 1396
Fragments arranged by size:
2179 1396 1183 947 812 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5335 5522 1700
Size: 110 3525 187 2869
Fragments arranged by size:
3525 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3024 3452 96
Size: 2928 428 3335
Fragments arranged by size:
3335 2928 428
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6691
BssSI C'ACGA_G
Cuts at: 4751 5763 4751
Size: 1012 5679
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6691
BstBI TT'CG_AA
Cuts at: 5495 5495
Size: 6691
BstEII G'GTnAC_C
Cuts at: 1184 2960 1184
Size: 1776 4915
BstYI r'GATC_y
Cuts at: 1972 2485 4021 4388 4773 4790 6242 6328 6340
Size: 513 1536 367 385 17 1452 86 12
Cuts at: 6340 6686 1972
Size: 346 1977
Fragments arranged by size:
1977 1536 1452 513 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2121 487
Size: 683 56 895 5057
Fragments arranged by size:
5057 895 683 56
BtsI GCAGTG_nn'
Cuts at: 2671 2839 3427 3847 3931 4207 5001 5021 2671
Size: 168 588 420 84 276 794 20 4341
Fragments arranged by size:
4341 794 588 420 276 168 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2989 3024 3157 3192 3325 3360 529
Size: 35 2425 35 133 35 133 35 3860
Fragments arranged by size:
3860 2425 133 133 35 35 35 35
DraI TTT'AAA
Cuts at: 2226 3995 2226
Size: 1769 4922
DraIII CAC_nnn'GTG
Cuts at: 3678 3678
Size: 6691
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5698 809
Size: 507 4382 1802
Fragments arranged by size:
4382 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 4138 5027
Size: 54 1425 50 275 18 213 2095 889
Cuts at: 5027 6656 8
Size: 1629 43
Fragments arranged by size:
2095 1629 1425 889 275 213 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4138 1537
Size: 275 2326 4090
Fragments arranged by size:
4090 2326 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 3455 3931 4621 1353
Size: 1155 947 476 690 3423
Fragments arranged by size:
3423 1155 947 690 476
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5278 5652 5798 387
Size: 1064 138 3689 374 146 1280
Fragments arranged by size:
3689 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2303 2748 2752 2832 2858 3000
Size: 393 506 165 445 4 80 26 142
Cuts at: 3000 3084 3168 3252 3336 3420 3479 3504 3840
Size: 84 84 84 84 84 59 25 336
Cuts at: 3840 3856 3924 4754 5351 6138 6406 1239
Size: 16 68 830 597 787 268 1524
Fragments arranged by size:
1524 830 787 597 506 445 393 336
268 165 142 84 84 84 84 84
80 68 59 26 25 16 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2942 2015
Size: 927 5764
EcoRI G'AATT_C
Cuts at: 2073 3558 3894 4000 4378 2073
Size: 1485 336 106 378 4386
Fragments arranged by size:
4386 1485 378 336 106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2870 2870
Size: 6691
FspI TGC'GCA
Cuts at: 5197 5197
Size: 6691
HaeII r_GCGC'y
Cuts at: 1678 5838 1678
Size: 4160 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3901 3933 5332 5364 5406
Size: 32 135 32 2329 32 1399 32 42
Cuts at: 5406 5438 1373
Size: 32 2626
Fragments arranged by size:
2626 2329 1399 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4035 4281 669
Size: 988 281 2097 246 3079
Fragments arranged by size:
3079 2097 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2339 2847 748
Size: 1591 508 4592
Fragments arranged by size:
4592 1591 508
HpaI GTT'AAC
Cuts at: 4281 4281
Size: 6691
KpnI G_GTAC'C
Cuts at: 4016 4016
Size: 6691
MfeI C'AATT_G
Cuts at: 4290 4290
Size: 6691
MluI A'CGCG_T
Cuts at: 6567 6567
Size: 6691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2721 5805 5989 1305
Size: 1416 3084 184 2007
Fragments arranged by size:
3084 2007 1416 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 10
Size: 54 1425 343 213 4656
Fragments arranged by size:
4656 1425 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2106 2450 3567 4649 5008 5167 518
Size: 648 940 344 1117 1082 359 159 2042
Fragments arranged by size:
2042 1117 1082 940 648 359 344 159
MspA1I CmG'CkG
Cuts at: 1606 2577 3114 3450 4107 4784 5932 6177 1606
Size: 971 537 336 657 677 1148 245 2120
Fragments arranged by size:
2120 1148 971 677 657 537 336 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6139
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6691
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6691
NotI GC'GGCC_GC
Cuts at: 4138 4138
Size: 6691
NsiI A_TGCA'T
Cuts at: 2453 2453
Size: 6691
NspI r_CATG'y
Cuts at: 1132 1229 2271 2451 2455 4049 5594 1132
Size: 97 1042 180 4 1594 1545 2229
Fragments arranged by size:
2229 1594 1545 1042 180 97 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6691
PciI A'CATG_T
Cuts at: 2267 5590 2267
Size: 3323 3368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6691
PmeI GTTT'AAAC
Cuts at: 3995 3995
Size: 6691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4760 4792 6273 6305 4760
Size: 32 1481 32 5146
Fragments arranged by size:
5146 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6691
PsiI TTA'TAA
Cuts at: 2742 4261 2742
Size: 1519 5172
PstI C_TGCA'G
Cuts at: 830 4043 830
Size: 3213 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6691
PvuII CAG'CTG
Cuts at: 2577 3114 3450 2577
Size: 537 336 5818
Fragments arranged by size:
5818 537 336
SalI G'TCGA_C
Cuts at: 1936 4033 1936
Size: 2097 4594
SapI GCTCTTCn'nnn_
Cuts at: 2508 2508
Size: 6691
SbfI CC_TGCA'GG
Cuts at: 4043 4043
Size: 6691
ScaI AGT'ACT
Cuts at: 4939 6651 4939
Size: 1712 4979
SfcI C'TryA_G
Cuts at: 826 1045 2199 4039 5174 5855 6046 826
Size: 219 1154 1840 1135 681 191 1471
Fragments arranged by size:
1840 1471 1154 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6691
SmaI CCC'GGG
Cuts at: 1872 4018 1872
Size: 2146 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4795 5696 5958 820
Size: 197 926 2852 901 262 1553
Fragments arranged by size:
2852 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4482 493
Size: 3989 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6691
SphI G_CATG'C
Cuts at: 1132 2455 4049 1132
Size: 1323 1594 3774
Fragments arranged by size:
3774 1594 1323
SspI AAT'ATT
Cuts at: 5 52 4615 5
Size: 47 4563 2081
Fragments arranged by size:
4563 2081 47
StuI AGG'CCT
Cuts at: 1517 2401 2549 2653 2737 2821 2905 2933 3073
Size: 884 148 104 84 84 84 28 140
Cuts at: 3073 3409 3577 1517
Size: 336 168 4631
Fragments arranged by size:
4631 884 336 168 148 140 104 84
84 84 28
StyI C'CwwG_G
Cuts at: 513 1065 2163 2900 4130 513
Size: 552 1098 737 1230 3074
Fragments arranged by size:
3074 1230 1098 737 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 2502 2597 4718 4735 4888 5073
Size: 1255 678 244 95 2121 17 153 185
Cuts at: 5073 5518 325
Size: 445 1498
Fragments arranged by size:
2121 1498 1255 678 445 244 185 153
95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3024 3452 4937
Size: 274 80 33 51 1443 1047 428 1485
Cuts at: 4937 6649 96
Size: 1712 138
Fragments arranged by size:
1712 1485 1443 1047 428 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6651 6683 6651
Size: 32 6659
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6691
XbaI T'CTAG_A
Cuts at: 4027 4027
Size: 6691
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6691
XmaI C'CCGG_G
Cuts at: 1870 4016 1870
Size: 2146 4545
XmnI GAAnn'nnTTC
Cuts at: 2933 4820 2933
Size: 1887 4804
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4499 276
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI