Restriction map for
pFN21AE0081
(Circular) (Six-base) MAPSORT of: pFN21AE0081.seq Check: 1639 from: 1 to: 8851
pFN21AE0081 8851 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2864 2864
Size: 8851
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6661 278
Size: 53 83 186 936 5125 2468
Fragments arranged by size:
5125 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4380 6194 1937
Size: 2443 1814 4594
Fragments arranged by size:
4594 2443 1814
Acc65I G'GTAC_C
Cuts at: 6172 6172
Size: 8851
AclI AA'CG_TT
Cuts at: 1563 6978 7351 1563
Size: 5415 373 3063
Fragments arranged by size:
5415 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2436 3241 4453 5079 6914 8298 8566 1632
Size: 804 805 1212 626 1835 1384 268 1917
Fragments arranged by size:
1917 1835 1384 1212 805 804 626 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8654
AflIII A'CryG_T
Cuts at: 1203 4224 7750 8727 1203
Size: 3021 3526 977 1327
Fragments arranged by size:
3526 3021 1327 977
AgeI A'CCGG_T
Cuts at: 2798 7682 2798
Size: 4884 3967
AhdI GACnn_n'nnGTC
Cuts at: 3985 5647 7580 3985
Size: 1662 1933 5256
Fragments arranged by size:
5256 1933 1662
AleI CACnn'nnGTG
Cuts at: 1166 6017 1166
Size: 4851 4000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8851
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2203 2235 2203
Size: 32 8819
AlwNI CAG_nnn'CTG
Cuts at: 2260 3157 4279 4476 4828 6594 8166 2260
Size: 897 1122 197 352 1766 1572 2945
Fragments arranged by size:
2945 1766 1572 1122 897 352 197
ApaI G_GGCC'C
Cuts at: 3578 3578
Size: 8851
ApaLI G'TGCA_C
Cuts at: 6908 8064 6908
Size: 1156 7695
ApoI r'AATT_y
Cuts at: 1493 3331 4094 6068 6160 6386 6538 8647 1493
Size: 1838 763 1974 92 226 152 2109 1697
Fragments arranged by size:
2109 1974 1838 1697 763 226 152 92
AseI AT'TA_AT
Cuts at: 160 1032 7405 160
Size: 872 6373 1606
Fragments arranged by size:
6373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8851
AvaI C'yCGr_G
Cuts at: 1870 1943 2540 3467 3545 3695 4507 6176 1870
Size: 73 597 927 78 150 812 1669 4545
Fragments arranged by size:
4545 1669 927 812 597 150 78 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5327 5360 5327
Size: 33 8818
BamHI G'GATC_C
Cuts at: 6181 6548 6181
Size: 367 8484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 6172 7627 618
Size: 325 217 627 242 4143 1455 1842
Fragments arranged by size:
4143 1842 1455 627 325 242 217
BanII G_rGCy'C
Cuts at: 1391 3383 3578 3704 4508 4608 5036 5540 5586
Size: 1992 195 126 804 100 428 504 46
Cuts at: 5586 6170 1391
Size: 584 4072
Fragments arranged by size:
4072 1992 804 584 504 428 195 126
100 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3108 4046 4061 4447 5067 928
Size: 971 1209 938 15 386 620 4712
Fragments arranged by size:
4712 1209 971 938 620 386 15
BbvCI CC'TCA_GC
Cuts at: 2471 2471
Size: 8851
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5974 6008 7042 7076 1903
Size: 34 4037 34 1034 34 3678
Fragments arranged by size:
4037 3678 1034 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2897 5328 6743 7953 2897
Size: 2431 1415 1210 3795
Fragments arranged by size:
3795 2431 1415 1210
BclI T'GATC_A
Cuts at: 1548 2850 1548
Size: 1302 7549
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6125 7462 136
Size: 107 122 71 631 605 4453 1337 1525
Fragments arranged by size:
4453 1525 1337 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3963 5742 8846 3963
Size: 1779 3104 3968
Fragments arranged by size:
3968 3104 1779
BlpI GC'TnA_GC
Cuts at: 2985 4484 6268 2985
Size: 1499 1784 5568
Fragments arranged by size:
5568 1784 1499
Bme1580I G_kGCm'C
Cuts at: 1790 2034 3487 3578 6912 8068 1790
Size: 244 1453 91 3334 1156 2573
Fragments arranged by size:
3334 2573 1453 1156 244 91
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2035 6582 7540
Size: 416 53 83 399 24 618 4547 958
Cuts at: 7540 442
Size: 1753
Fragments arranged by size:
4547 1753 958 618 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8851
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2633 3560 4522 2633
Size: 927 962 6962
Fragments arranged by size:
6962 962 927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2145 2152 2653 3356 3509 3515 3698 3800
Size: 906 7 501 703 153 6 183 102
Cuts at: 3800 4425 4994 5174 5657 7511 1239
Size: 625 569 180 483 1854 2579
Fragments arranged by size:
2579 1854 906 703 625 569 501 483
183 180 153 102 7 6
Bpu10I CC'TnA_GC
Cuts at: 2471 5030 2471
Size: 2559 6292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2227 2413 4387 5655 6976 7841 8139 2227
Size: 186 1974 1268 1321 865 298 2939
Fragments arranged by size:
2939 1974 1321 1268 865 298 186
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3329 4423 4995 7514 882
Size: 617 1830 1094 572 2519 2219
Fragments arranged by size:
2519 2219 1830 1094 617 572
BsaAI yAC'GTr
Cuts at: 493 1204 4006 6642 493
Size: 711 2802 2636 2702
Fragments arranged by size:
2802 2702 2636 711
BsaBI GATnn'nnATC
Cuts at: 8845 8845
Size: 8851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3999 5267 6658 7040
Size: 53 83 186 936 2466 1268 1391 382
Cuts at: 7040 7675 275
Size: 635 1451
Fragments arranged by size:
2466 1451 1391 1268 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2798 7284 7682 7956 8103 8514 2798
Size: 4486 398 274 147 411 3135
Fragments arranged by size:
4486 3135 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2341 2371 3818 3848 1178
Size: 30 165 30 938 30 1447 30 6181
Fragments arranged by size:
6181 1447 938 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2656 3449 3695 4040 4358 4361 4610 4633
Size: 1470 793 246 345 318 3 249 23
Cuts at: 4633 1186
Size: 5404
Fragments arranged by size:
5404 1470 793 345 318 249 246 23
3
BseYI C'CCAG_C
Cuts at: 2253 3413 3738 8054 8550 2253
Size: 1160 325 4316 496 2554
Fragments arranged by size:
4316 2554 1160 496 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3734 4673 5968 1163
Size: 573 1998 939 1295 4046
Fragments arranged by size:
4046 1998 1295 939 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3958 4062 6080 6301 7062 8090
Size: 275 186 1957 104 2018 221 761 1028
Cuts at: 8090 1540
Size: 2301
Fragments arranged by size:
2301 2018 1957 1028 761 275 221 186
104
BsiHKAI G_wGCw'C
Cuts at: 3825 5036 5994 6912 6997 8068 3825
Size: 1211 958 918 85 1071 4608
Fragments arranged by size:
4608 1211 1071 958 918 85
BsmI GAATG_Cn'
Cuts at: 6362 6455 6362
Size: 93 8758
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 3383 3487 3578 3704 3825 4508
Size: 399 244 1349 104 91 126 121 683
Cuts at: 4508 4608 5036 5540 5586 5994 6170 6912 6997
Size: 100 428 504 46 408 176 742 85
Cuts at: 6997 8068 1391
Size: 1071 2174
Fragments arranged by size:
2174 1349 1071 742 683 504 428 408
399 244 176 126 121 104 100 91
85 46
BspEI T'CCGG_A
Cuts at: 8514 8514
Size: 8851
BspHI T'CATG_A
Cuts at: 2313 2961 5160 6740 8470 2313
Size: 648 2199 1580 1730 2694
Fragments arranged by size:
2694 2199 1730 1580 648
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2864 3978 5118 6206 844
Size: 2020 1114 1140 1088 3489
Fragments arranged by size:
3489 2020 1140 1114 1088
BsrBI CCG'CTC
Cuts at: 1394 1823 2311 3892 6738 1394
Size: 429 488 1581 2846 3507
Fragments arranged by size:
3507 2846 1581 488 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2526 4540 4560 5865 5997 6058 7346
Size: 1183 1278 2014 20 1305 132 61 1288
Cuts at: 7346 7520 65
Size: 174 1396
Fragments arranged by size:
2014 1396 1305 1288 1278 1183 174 132
61 20
BsrFI r'CCGG_y
Cuts at: 1700 1810 2798 4755 4986 7495 7682 1700
Size: 110 988 1957 231 2509 187 2869
Fragments arranged by size:
2869 2509 1957 988 231 187 110
BsrGI T'GTAC_A
Cuts at: 96 3066 96
Size: 2970 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8851
BssSI C'ACGA_G
Cuts at: 2051 4666 6911 7923 2051
Size: 2615 2245 1012 2979
Fragments arranged by size:
2979 2615 2245 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2918 794
Size: 2124 6727
BstBI TT'CG_AA
Cuts at: 7655 7655
Size: 8851
BstEII G'GTnAC_C
Cuts at: 1184 4069 1184
Size: 2885 5966
BstXI CCAn_nnnn'nTGG
Cuts at: 4291 4403 4912 5048 5932 4291
Size: 112 509 136 884 7210
Fragments arranged by size:
7210 884 509 136 112
BstYI r'GATC_y
Cuts at: 1972 2565 3963 5674 5713 5742 6181 6548 6933
Size: 593 1398 1711 39 29 439 367 385
Cuts at: 6933 6950 8402 8488 8500 8846 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1711 1452 1398 593 439 385 367
346 86 39 29 17 12
Bsu36I CC'TnA_GG
Cuts at: 2154 2773 4645 2154
Size: 619 1872 6360
Fragments arranged by size:
6360 1872 619
BtgI C'CryG_G
Cuts at: 513 1065 2183 2605 4542 4680 5500 5586 513
Size: 552 1118 422 1937 138 820 86 3778
Fragments arranged by size:
3778 1937 1118 820 552 422 138 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2091 2847 487
Size: 683 56 865 756 6491
Fragments arranged by size:
6491 865 756 683 56
BtsI GCAGTG_nn'
Cuts at: 2907 3351 3826 5283 6367 7161 7181 2907
Size: 444 475 1457 1084 794 20 4577
Fragments arranged by size:
4577 1457 1084 794 475 444 20
ClaI AT'CG_AT
Cuts at: 1554 5907 1554
Size: 4353 4498
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8816
DraI TTT'AAA
Cuts at: 5233 6155 5233
Size: 922 7929
DraIII CAC_nnn'GTG
Cuts at: 5512 5512
Size: 8851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2390 7858 809
Size: 507 1074 5468 1802
Fragments arranged by size:
5468 1802 1074 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3192 4102 4281
Size: 54 1425 50 275 18 1362 910 179
Cuts at: 4281 5408 5503 6077 6126 6298 7187 8816 8
Size: 1127 95 574 49 172 889 1629 43
Fragments arranged by size:
1629 1425 1362 1127 910 889 574 275
179 172 95 54 50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6077 6298 1537
Size: 275 4265 221 4090
Fragments arranged by size:
4265 4090 275 221
EarI CTCTTCn'nnn_
Cuts at: 1353 2009 2044 2577 4290 4706 5573 6781 1353
Size: 656 35 533 1713 416 867 1208 3423
Fragments arranged by size:
3423 1713 1208 867 656 533 416 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5561 7438 7812 7958 387
Size: 1064 138 3972 1877 374 146 1280
Fragments arranged by size:
3972 1877 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5034 5034
Size: 8851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2145 2152 2436 2653 3241 3356 3509
Size: 393 513 7 284 217 588 115 153
Cuts at: 3509 3515 3698 3800 4425 4453 4994 5079 5174
Size: 6 183 102 625 28 541 85 95
Cuts at: 5174 5657 6914 7511 8298 8566 1239
Size: 483 1257 597 787 268 1524
Fragments arranged by size:
1524 1257 787 625 597 588 541 513
483 393 284 268 217 183 153 115
102 95 85 28 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 5758 5774 5758
Size: 16 8835
EcoO109I rG'GnC_Cy
Cuts at: 3847 3895 3847
Size: 48 8803
EcoRI G'AATT_C
Cuts at: 3331 6160 6538 3331
Size: 2829 378 5644
Fragments arranged by size:
5644 2829 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5569 5569
Size: 8851
FspI TGC'GCA
Cuts at: 2860 5097 7357 2860
Size: 2237 2260 4354
Fragments arranged by size:
4354 2260 2237
HaeII r_GCGC'y
Cuts at: 1678 7998 1678
Size: 6320 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2769 2801 3971 4003 4893
Size: 32 135 32 1197 32 1170 32 890
Cuts at: 4893 4925 5633 5665 7492 7524 7566 7598 1373
Size: 32 708 32 1827 32 42 32 2626
Fragments arranged by size:
2626 1827 1197 1170 890 708 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5248 6195 6441 669
Size: 988 281 3310 947 246 3079
Fragments arranged by size:
3310 3079 988 947 281 246
HindIII A'AGCT_T
Cuts at: 748 2323 2448 748
Size: 1575 125 7151
Fragments arranged by size:
7151 1575 125
HpaI GTT'AAC
Cuts at: 6441 6441
Size: 8851
KpnI G_GTAC'C
Cuts at: 6176 6176
Size: 8851
MfeI C'AATT_G
Cuts at: 6450 6450
Size: 8851
MluI A'CGCG_T
Cuts at: 8727 8727
Size: 8851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2654 3978 4098 4352 4476 7965 8149 1305
Size: 1349 1324 120 254 124 3489 184 2007
Fragments arranged by size:
3489 2007 1349 1324 254 184 124 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3194 4104 4283 5410 6128
Size: 54 1425 343 1362 910 179 1127 718
Cuts at: 6128 10
Size: 2733
Fragments arranged by size:
2733 1425 1362 1127 910 718 343 179
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3023 3782 4628 4910 4958 5046 5351
Size: 648 1857 759 846 282 48 88 305
Cuts at: 5351 5811 5930 6017 6809 7168 7327 518
Size: 460 119 87 792 359 159 2042
Fragments arranged by size:
2042 1857 846 792 759 648 460 359
305 282 159 119 88 87 48
MspA1I CmG'CkG
Cuts at: 1606 2174 2714 3200 3759 4081 4403 4594 4719
Size: 568 540 486 559 322 322 191 125
Cuts at: 4719 4792 5263 5502 5857 6267 6944 8092 8337
Size: 73 471 239 355 410 677 1148 245
Cuts at: 8337 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 568 559 540 486 471
410 355 322 322 245 239 191 125
73
NaeI GCC'GGC
Cuts at: 4757 4757
Size: 8851
NcoI C'CATG_G
Cuts at: 513 1065 2605 4542 5586 513
Size: 552 1540 1937 1044 3778
Fragments arranged by size:
3778 1937 1540 1044 552
NdeI CA'TA_TG
Cuts at: 387 3796 387
Size: 3409 5442
NgoMIV G'CCGG_C
Cuts at: 4755 4755
Size: 8851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8851
NotI GC'GGCC_GC
Cuts at: 6298 6298
Size: 8851
NspI r_CATG'y
Cuts at: 1132 1229 3265 3512 4228 6209 7754 1132
Size: 97 2036 247 716 1981 1545 2229
Fragments arranged by size:
2229 2036 1981 1545 716 247 97
PasI CC'CwG_GG
Cuts at: 1348 5401 1348
Size: 4053 4798
PciI A'CATG_T
Cuts at: 4224 7750 4224
Size: 3526 5325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2260 2611 5136 5417 5512 6040 1108
Size: 1152 351 2525 281 95 528 3919
Fragments arranged by size:
3919 2525 1152 528 351 281 95
PfoI T'CCnGG_A
Cuts at: 2568 3372 3675 2568
Size: 804 303 7744
Fragments arranged by size:
7744 804 303
PmeI GTTT'AAAC
Cuts at: 6155 6155
Size: 8851
PmlI CAC'GTG
Cuts at: 4006 4006
Size: 8851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5300 5332 6920 6952 8433 8465 5300
Size: 32 1588 32 1481 32 5686
Fragments arranged by size:
5686 1588 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3847 3847
Size: 8851
PshAI GACnn'nnGTC
Cuts at: 1613 4061 1613
Size: 2448 6403
PsiI TTA'TAA
Cuts at: 2103 6421 2103
Size: 4318 4533
PspOMI G'GGCC_C
Cuts at: 3574 3574
Size: 8851
PspXI vC'TCGA_Gb
Cuts at: 3695 3695
Size: 8851
PstI C_TGCA'G
Cuts at: 830 2879 3346 4086 5262 5343 6203 830
Size: 2049 467 740 1176 81 860 3478
Fragments arranged by size:
3478 2049 1176 860 740 467 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8851
PvuII CAG'CTG
Cuts at: 2174 2714 4081 4403 4594 4792 5263 5857 2174
Size: 540 1367 322 191 198 471 594 5168
Fragments arranged by size:
5168 1367 594 540 471 322 198 191
SacI G_AGCT'C
Cuts at: 5036 5036
Size: 8851
SacII CC_GC'GG
Cuts at: 5503 5503
Size: 8851
SalI G'TCGA_C
Cuts at: 1936 6193 1936
Size: 4257 4594
SapI GCTCTTCn'nnn_
Cuts at: 2577 4706 2577
Size: 2129 6722
SbfI CC_TGCA'GG
Cuts at: 6203 6203
Size: 8851
ScaI AGT'ACT
Cuts at: 2847 4088 7099 8811 2847
Size: 1241 3011 1712 2887
Fragments arranged by size:
3011 2887 1712 1241
SexAI A'CCwGG_T
Cuts at: 3597 3597
Size: 8851
SfcI C'TryA_G
Cuts at: 826 1045 2875 3342 4082 4981 5258 5325 5339
Size: 219 1830 467 740 899 277 67 14
Cuts at: 5339 6199 7334 8015 8206 826
Size: 860 1135 681 191 1471
Fragments arranged by size:
1830 1471 1135 899 860 740 681 467
277 219 191 67 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8851
SgrAI Cr'CCGG_yG
Cuts at: 1700 2798 1700
Size: 1098 7753
SmaI CCC'GGG
Cuts at: 1872 3469 6178 1872
Size: 1597 2709 4545
Fragments arranged by size:
4545 2709 1597
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2206 2428 3695 4366 5670 6955
Size: 197 926 263 222 1267 671 1304 1285
Cuts at: 6955 7856 8118 820
Size: 901 262 1553
Fragments arranged by size:
1553 1304 1285 1267 926 901 671 263
262 222 197
SnaBI TAC'GTA
Cuts at: 493 6642 493
Size: 6149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8851
SphI G_CATG'C
Cuts at: 1132 3265 3512 6209 1132
Size: 2133 247 2697 3774
Fragments arranged by size:
3774 2697 2133 247
SspI AAT'ATT
Cuts at: 5 52 2550 2576 6775 5
Size: 47 2498 26 4199 2081
Fragments arranged by size:
4199 2498 2081 47 26
StuI AGG'CCT
Cuts at: 1517 3532 3746 5028 1517
Size: 2015 214 1282 5340
Fragments arranged by size:
5340 2015 1282 214
StyI C'CwwG_G
Cuts at: 513 1065 2605 3609 4542 4659 5178 5553 5586
Size: 552 1540 1004 933 117 519 375 33
Cuts at: 5586 5754 5775 6290 513
Size: 168 21 515 3074
Fragments arranged by size:
3074 1540 1004 933 552 519 515 375
168 117 33 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2017 5482 6878 6895 7048 7233 7678
Size: 1255 437 3465 1396 17 153 185 445
Cuts at: 7678 325
Size: 1498
Fragments arranged by size:
3465 1498 1396 1255 445 437 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2536 2845 3066
Size: 274 80 33 51 1443 559 309 221
Cuts at: 3066 4086 5343 7097 8809 96
Size: 1020 1257 1754 1712 138
Fragments arranged by size:
1754 1712 1443 1257 1020 559 309 274
221 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2341 2352 2373 2384 3818 3850 4120 4152 4302
Size: 11 21 11 1434 32 270 32 150
Cuts at: 4302 4334 5380 5412 8811 8843 2341
Size: 32 1046 32 3399 32 2349
Fragments arranged by size:
3399 2349 1434 1046 270 150 32 32
32 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8851
XbaI T'CTAG_A
Cuts at: 6187 6187
Size: 8851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3421 4549 5137 3421
Size: 1128 588 7135
Fragments arranged by size:
7135 1128 588
XhoI C'TCGA_G
Cuts at: 1943 3695 1943
Size: 1752 7099
XmaI C'CCGG_G
Cuts at: 1870 3467 6176 1870
Size: 1597 2709 4545
Fragments arranged by size:
4545 2709 1597
XmnI GAAnn'nnTTC
Cuts at: 3365 4769 6980 3365
Size: 1404 2211 5236
Fragments arranged by size:
5236 2211 1404
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6659 276
Size: 53 83 186 936 5125 2468
Fragments arranged by size:
5125 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PsrI RsrII SanDI SfiI SfoI SrfI SwaI