Restriction map for
pFN21AE0079
(Circular) (Six-base) MAPSORT of: pFN21AE0079.seq Check: 3658 from: 1 to: 6553
pFN21AE0079 6553 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2068 3515 3607 2068
Size: 1447 92 5014
Fragments arranged by size:
5014 1447 92
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4363 278
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3651 3896 1937
Size: 1714 245 4594
Fragments arranged by size:
4594 1714 245
Acc65I G'GTAC_C
Cuts at: 2355 3874 2355
Size: 1519 5034
AclI AA'CG_TT
Cuts at: 1563 4680 5053 1563
Size: 3117 373 3063
Fragments arranged by size:
3117 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3007 3465 4616 6000 6268 1632
Size: 1375 458 1151 1384 268 1917
Fragments arranged by size:
1917 1384 1375 1151 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6356
AflIII A'CryG_T
Cuts at: 1203 2169 2936 5452 6429 1203
Size: 966 767 2516 977 1327
Fragments arranged by size:
2516 1327 977 966 767
AgeI A'CCGG_T
Cuts at: 5384 5384
Size: 6553
AhdI GACnn_n'nnGTC
Cuts at: 5282 5282
Size: 6553
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3355 1184
Size: 2171 4382
AlwNI CAG_nnn'CTG
Cuts at: 2517 3085 4296 5868 2517
Size: 568 1211 1572 3202
Fragments arranged by size:
3202 1572 1211 568
ApaLI G'TGCA_C
Cuts at: 2344 4610 5766 2344
Size: 2266 1156 3131
Fragments arranged by size:
3131 2266 1156
ApoI r'AATT_y
Cuts at: 1493 3862 4088 4240 6349 1493
Size: 2369 226 152 2109 1697
Fragments arranged by size:
2369 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3731 3731
Size: 6553
AseI AT'TA_AT
Cuts at: 160 1032 5107 160
Size: 872 4075 1606
Fragments arranged by size:
4075 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
AvaI C'yCGr_G
Cuts at: 1870 1943 3482 3788 3817 3878 1870
Size: 73 1539 306 29 61 4545
Fragments arranged by size:
4545 1539 306 73 61 29
BamHI G'GATC_C
Cuts at: 3883 4250 3883
Size: 367 6186
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 2355 2417 3527 3874
Size: 325 217 627 238 330 62 1110 347
Cuts at: 3874 5329 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1110 627 347 330 325 238
217 62
BanII G_rGCy'C
Cuts at: 1391 3225 3699 3872 1391
Size: 1834 474 173 4072
Fragments arranged by size:
4072 1834 474 173
BbeI G_GCGC'C
Cuts at: 2029 2421 2029
Size: 392 6161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2166 2888 3021 928
Size: 971 267 722 133 4460
Fragments arranged by size:
4460 971 722 267 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 4744 4778 1903
Size: 34 1871 34 902 34 3678
Fragments arranged by size:
3678 1871 902 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4445 5655 4445
Size: 1210 5343
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6553
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2300 2472 2681
Size: 107 122 71 631 605 628 172 209
Cuts at: 2681 5164 136
Size: 2483 1525
Fragments arranged by size:
2483 1525 631 628 605 209 172 122
107 71
BglII A'GATC_T
Cuts at: 6548 6548
Size: 6553
BlpI GC'TnA_GC
Cuts at: 3514 3970 3514
Size: 456 6097
Bme1580I G_kGCm'C
Cuts at: 1790 2348 2936 4614 5770 1790
Size: 558 588 1678 1156 2573
Fragments arranged by size:
2573 1678 1156 588 558
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6553
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3313 3337 3409
Size: 416 53 83 399 24 1896 24 72
Cuts at: 3409 4284 5242 442
Size: 875 958 1753
Fragments arranged by size:
1896 1753 958 875 416 399 83 72
53 24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6553
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3832 3832
Size: 6553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2383 2894 3158 3592 5213 1239
Size: 1144 511 264 434 1621 2579
Fragments arranged by size:
2579 1621 1144 511 434 264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4678 5543 5841 4678
Size: 865 298 5390
Fragments arranged by size:
5390 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3468 3716 5216 882
Size: 617 1969 248 1500 2219
Fragments arranged by size:
2219 1969 1500 617 248
BsaAI yAC'GTr
Cuts at: 493 1204 4344 493
Size: 711 3140 2702
Fragments arranged by size:
3140 2702 711
BsaBI GATnn'nnATC
Cuts at: 6547 6547
Size: 6553
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2026 2418 4360 4742
Size: 53 83 186 936 493 392 1942 382
Cuts at: 4742 5377 275
Size: 635 1451
Fragments arranged by size:
1942 1451 936 635 493 392 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4986 5384 5658 5805 6216 4986
Size: 398 274 147 411 5323
Fragments arranged by size:
5323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3047 3077 3578 3608 1178
Size: 30 165 30 1644 30 501 30 4123
Fragments arranged by size:
4123 1644 501 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2088 2891 3734 1186
Size: 902 803 843 4005
Fragments arranged by size:
4005 902 843 803
BseYI C'CCAG_C
Cuts at: 2351 2421 3066 3317 5756 6252 2351
Size: 70 645 251 2439 496 2652
Fragments arranged by size:
2652 2439 645 496 251 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3199 3250 3325 3734 3848 1163
Size: 573 1463 51 75 409 114 3868
Fragments arranged by size:
3868 1463 573 409 114 75 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4003 4764 5792 1540
Size: 275 186 2002 761 1028 2301
Fragments arranged by size:
2301 2002 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2348 3191 4614 4699 5770 2348
Size: 843 1423 85 1071 3131
Fragments arranged by size:
3131 1423 1071 843 85
BsmI GAATG_Cn'
Cuts at: 4064 4157 4064
Size: 93 6460
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2348 2936 3191 3225 3699 3872 4614
Size: 399 558 588 255 34 474 173 742
Cuts at: 4614 4699 5770 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 588 558 474 399 255
173 85 34
BspEI T'CCGG_A
Cuts at: 6216 6216
Size: 6553
BspHI T'CATG_A
Cuts at: 4442 6172 4442
Size: 1730 4823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2068 3515 3607 3908 844
Size: 1224 1447 92 301 3489
Fragments arranged by size:
3489 1447 1224 301 92
BsrBI CCG'CTC
Cuts at: 1394 1823 4440 1394
Size: 429 2617 3507
Fragments arranged by size:
3507 2617 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3292 5048 5222 65
Size: 1183 2044 1756 174 1396
Fragments arranged by size:
2044 1756 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2312 3823 5197 5384 1700
Size: 110 502 1511 1374 187 2869
Fragments arranged by size:
2869 1511 1374 502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6553
BssHII G'CGCG_C
Cuts at: 1925 2014 2058 2403 3731 1925
Size: 89 44 345 1328 4747
Fragments arranged by size:
4747 1328 345 89 44
BssSI C'ACGA_G
Cuts at: 3383 4613 5625 3383
Size: 1230 1012 4311
Fragments arranged by size:
4311 1230 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2431 2463 3348 794
Size: 1637 32 885 3999
Fragments arranged by size:
3999 1637 885 32
BstBI TT'CG_AA
Cuts at: 5357 5357
Size: 6553
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6553
BstYI r'GATC_y
Cuts at: 1972 3883 4250 4635 4652 6104 6190 6202 6548
Size: 1911 367 385 17 1452 86 12 346
Cuts at: 6548 1972
Size: 1977
Fragments arranged by size:
1977 1911 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2794 2794
Size: 6553
BtgI C'CryG_G
Cuts at: 513 1065 2004 2304 2499 3699 513
Size: 552 939 300 195 1200 3367
Fragments arranged by size:
3367 1200 939 552 300 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5814
Fragments arranged by size:
5814 683 56
BtsI GCAGTG_nn'
Cuts at: 4069 4863 4883 4069
Size: 794 20 5739
Fragments arranged by size:
5739 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6553
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6518
DraI TTT'AAA
Cuts at: 3857 3857
Size: 6553
DraIII CAC_nnn'GTG
Cuts at: 2400 2454 2400
Size: 54 6499
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2807 3250 5560 809
Size: 507 1491 443 2310 1802
Fragments arranged by size:
2310 1802 1491 507 443
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2767 3289
Size: 54 1425 50 275 18 471 466 522
Cuts at: 3289 3553 4000 4889 6518 8
Size: 264 447 889 1629 43
Fragments arranged by size:
1629 1425 889 522 471 466 447 275
264 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4000 1537
Size: 275 2188 4090
Fragments arranged by size:
4090 2188 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 4483 1353
Size: 1313 1817 3423
Fragments arranged by size:
3423 1817 1313
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5140 5514 5660 387
Size: 1064 138 3551 374 146 1280
Fragments arranged by size:
3551 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2383 2894 3007 3158 3465 3592 4616
Size: 393 751 511 113 151 307 127 1024
Cuts at: 4616 5213 6000 6268 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1024 787 751 597 511 393 307
268 151 127 113
EcoO109I rG'GnC_Cy
Cuts at: 2293 3060 2293
Size: 767 5786
EcoRI G'AATT_C
Cuts at: 3862 4240 3862
Size: 378 6175
FspI TGC'GCA
Cuts at: 5059 5059
Size: 6553
HaeII r_GCGC'y
Cuts at: 1678 2029 2338 2421 5700 1678
Size: 351 309 83 3279 2531
Fragments arranged by size:
3279 2531 351 309 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2083 2115 2774 2806 3047
Size: 32 135 32 511 32 659 32 241
Cuts at: 3047 3079 5194 5226 5268 5300 1373
Size: 32 2115 32 42 32 2626
Fragments arranged by size:
2626 2115 659 511 241 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3897 4143 669
Size: 988 281 1959 246 3079
Fragments arranged by size:
3079 1959 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6553
HpaI GTT'AAC
Cuts at: 4143 4143
Size: 6553
KasI G'GCGC_C
Cuts at: 2025 2417 2025
Size: 392 6161
KpnI G_GTAC'C
Cuts at: 2359 3878 2359
Size: 1519 5034
MfeI C'AATT_G
Cuts at: 4152 4152
Size: 6553
MluI A'CGCG_T
Cuts at: 6429 6429
Size: 6553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5667 5851 1305
Size: 4362 184 2007
Fragments arranged by size:
4362 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3291 3555 10
Size: 54 1425 343 1459 264 3008
Fragments arranged by size:
3008 1459 1425 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3644 4511 4870 5029 518
Size: 648 2478 867 359 159 2042
Fragments arranged by size:
2478 2042 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 3612 3969 4646 5794 6039 1606
Size: 429 1577 357 677 1148 245 2120
Fragments arranged by size:
2120 1577 1148 677 429 357 245
NaeI GCC'GGC
Cuts at: 2314 2314
Size: 6553
NarI GG'CG_CC
Cuts at: 2026 2418 2026
Size: 392 6161
NcoI C'CATG_G
Cuts at: 513 1065 2004 3699 513
Size: 552 939 1695 3367
Fragments arranged by size:
3367 1695 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6553
NgoMIV G'CCGG_C
Cuts at: 2312 2312
Size: 6553
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6553
NotI GC'GGCC_GC
Cuts at: 4000 4000
Size: 6553
NspI r_CATG'y
Cuts at: 1132 1229 2173 2850 2940 3911 5456 1132
Size: 97 944 677 90 971 1545 2229
Fragments arranged by size:
2229 1545 971 944 677 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6553
PciI A'CATG_T
Cuts at: 2169 2936 5452 2169
Size: 767 2516 3270
Fragments arranged by size:
3270 2516 767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3401 1108
Size: 2293 4260
PfoI T'CCnGG_A
Cuts at: 3768 3768
Size: 6553
PmeI GTTT'AAAC
Cuts at: 3857 3857
Size: 6553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4622 4654 6135 6167 4622
Size: 32 1481 32 5008
Fragments arranged by size:
5008 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6553
PsiI TTA'TAA
Cuts at: 4123 4123
Size: 6553
PspXI vC'TCGA_Gb
Cuts at: 3817 3817
Size: 6553
PstI C_TGCA'G
Cuts at: 830 2079 3905 830
Size: 1249 1826 3478
Fragments arranged by size:
3478 1826 1249
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6553
SalI G'TCGA_C
Cuts at: 1936 3895 1936
Size: 1959 4594
SbfI CC_TGCA'GG
Cuts at: 3905 3905
Size: 6553
ScaI AGT'ACT
Cuts at: 4801 6513 4801
Size: 1712 4841
SfcI C'TryA_G
Cuts at: 826 1045 2075 2707 3901 5036 5717 5908 826
Size: 219 1030 632 1194 1135 681 191 1471
Fragments arranged by size:
1471 1194 1135 1030 681 632 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2300 2300
Size: 6553
SfoI GGC'GCC
Cuts at: 2027 2419 2027
Size: 392 6161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6553
SmaI CCC'GGG
Cuts at: 1872 3880 1872
Size: 2008 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3817 4657 5558 5820 820
Size: 197 926 1874 840 901 262 1553
Fragments arranged by size:
1874 1553 926 901 840 262 197
SnaBI TAC'GTA
Cuts at: 493 4344 493
Size: 3851 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6553
SphI G_CATG'C
Cuts at: 1132 3911 1132
Size: 2779 3774
SspI AAT'ATT
Cuts at: 5 52 4477 5
Size: 47 4425 2081
Fragments arranged by size:
4425 2081 47
StuI AGG'CCT
Cuts at: 1517 3815 1517
Size: 2298 4255
StyI C'CwwG_G
Cuts at: 513 1065 2004 2536 3699 3846 3992 513
Size: 552 939 532 1163 147 146 3074
Fragments arranged by size:
3074 1163 939 552 532 147 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3017 4580 4597 4750 4935 5380 325
Size: 1255 1437 1563 17 153 185 445 1498
Fragments arranged by size:
1563 1498 1437 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2813 4799
Size: 274 80 33 51 1443 189 647 1986
Cuts at: 4799 6511 96
Size: 1712 138
Fragments arranged by size:
1986 1712 1443 647 274 189 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2363 2395 2438 2470 6513 6545 2363
Size: 32 43 32 4043 32 2371
Fragments arranged by size:
4043 2371 43 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6553
XbaI T'CTAG_A
Cuts at: 3889 3889
Size: 6553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3422 3485 3422
Size: 63 6490
XhoI C'TCGA_G
Cuts at: 1943 3817 1943
Size: 1874 4679
XmaI C'CCGG_G
Cuts at: 1870 3878 1870
Size: 2008 4545
XmnI GAAnn'nnTTC
Cuts at: 4682 4682
Size: 6553
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4361 276
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SrfI SwaI