Restriction map for
pFN21AE0077
(Circular) (Six-base) MAPSORT of: pFN21AE0077.seq Check: 174 from: 1 to: 8314
pFN21AE0077 8314 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6124 278
Size: 53 83 186 936 4588 2468
Fragments arranged by size:
4588 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5657 1937
Size: 3720 4594
Acc65I G'GTAC_C
Cuts at: 5635 5635
Size: 8314
AclI AA'CG_TT
Cuts at: 1563 6441 6814 1563
Size: 4878 373 3063
Fragments arranged by size:
4878 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 3198 3895 4276 6377 7761 8029 1632
Size: 482 1084 697 381 2101 1384 268 1917
Fragments arranged by size:
2101 1917 1384 1084 697 482 381 268
AfeI AGC'GCT
Cuts at: 4289 4289
Size: 8314
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8117
AflIII A'CryG_T
Cuts at: 1203 7213 8190 1203
Size: 6010 977 1327
Fragments arranged by size:
6010 1327 977
AgeI A'CCGG_T
Cuts at: 7145 7145
Size: 8314
AhdI GACnn_n'nnGTC
Cuts at: 7043 7043
Size: 8314
AleI CACnn'nnGTG
Cuts at: 1166 4121 1166
Size: 2955 5359
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2981 3147 4001 4364 5196 1184
Size: 1797 166 854 363 832 4302
Fragments arranged by size:
4302 1797 854 832 363 166
AlwNI CAG_nnn'CTG
Cuts at: 2131 2293 2347 2668 3379 3717 3742 3754 4876
Size: 162 54 321 711 338 25 12 1122
Cuts at: 4876 5537 5609 6057 7629 2131
Size: 661 72 448 1572 2816
Fragments arranged by size:
2816 1572 1122 711 661 448 338 321
162 72 54 25 12
ApaI G_GGCC'C
Cuts at: 2209 4459 4795 5351 2209
Size: 2250 336 556 5172
Fragments arranged by size:
5172 2250 556 336
ApaLI G'TGCA_C
Cuts at: 4829 4924 6371 7527 4829
Size: 95 1447 1156 5616
Fragments arranged by size:
5616 1447 1156 95
ApoI r'AATT_y
Cuts at: 1493 2870 5623 5849 6001 8110 1493
Size: 1377 2753 226 152 2109 1697
Fragments arranged by size:
2753 2109 1697 1377 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6868 160
Size: 872 5836 1606
Fragments arranged by size:
5836 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8314
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 4158 4562 4794 5639 1870
Size: 73 95 2120 404 232 845 4545
Fragments arranged by size:
4545 2120 845 404 232 95 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5357 5390 5357
Size: 33 8281
BamHI G'GATC_C
Cuts at: 2154 2653 5644 6011 2154
Size: 499 2991 367 4457
Fragments arranged by size:
4457 2991 499 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3455 3500 3672 3874 4153
Size: 325 217 627 1668 45 172 202 279
Cuts at: 4153 4163 4333 5516 5635 7090 618
Size: 10 170 1183 119 1455 1842
Fragments arranged by size:
1842 1668 1455 1183 627 325 279 217
202 172 170 119 45 10
BanII G_rGCy'C
Cuts at: 1391 2209 2609 3333 3902 3909 4459 4569 4795
Size: 818 400 724 569 7 550 110 226
Cuts at: 4795 5351 5547 5633 1391
Size: 556 196 86 4072
Fragments arranged by size:
4072 818 724 569 556 550 400 226
196 110 86 7
BbeI G_GCGC'C
Cuts at: 3676 3676
Size: 8314
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3631 4766 928
Size: 971 1732 1135 4476
Fragments arranged by size:
4476 1732 1135 971
BbvCI CC'TCA_GC
Cuts at: 2936 3653 2936
Size: 717 7597
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3490 3524 4915 4949 6505 6539 1903
Size: 34 1553 34 1391 34 1556 34 3678
Fragments arranged by size:
3678 1556 1553 1391 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6206 7416 6206
Size: 1210 7104
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2204 3098 4331
Size: 107 122 71 631 605 532 894 1233
Cuts at: 4331 4628 4820 6925 136
Size: 297 192 2105 1525
Fragments arranged by size:
2105 1525 1233 894 631 605 532 297
192 122 107 71
BglII A'GATC_T
Cuts at: 8309 8309
Size: 8314
BlpI GC'TnA_GC
Cuts at: 4710 5731 4710
Size: 1021 7293
Bme1580I G_kGCm'C
Cuts at: 1790 2209 2797 3340 3503 3879 3997 4166 4459
Size: 419 588 543 163 376 118 169 293
Cuts at: 4459 4795 4833 4928 5351 6375 7531 1790
Size: 336 38 95 423 1024 1156 2573
Fragments arranged by size:
2573 1156 1024 588 543 423 419 376
336 293 169 163 118 95 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3256 3905 4411
Size: 416 53 83 399 24 1839 649 506
Cuts at: 4411 4723 5619 6045 7003 442
Size: 312 896 426 958 1753
Fragments arranged by size:
1839 1753 958 896 649 506 426 416
399 312 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8314
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2612 2612
Size: 8314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2396 2690 3052 3449 3694 3939 4031 4506
Size: 1157 294 362 397 245 245 92 475
Cuts at: 4506 4739 6974 1239
Size: 233 2235 2579
Fragments arranged by size:
2579 2235 1157 475 397 362 294 245
245 233 92
Bpu10I CC'TnA_GC
Cuts at: 2936 3653 3726 4059 4100 4184 4196 5426 2936
Size: 717 73 333 41 84 12 1230 5824
Fragments arranged by size:
5824 1230 717 333 84 73 41 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 4286 5189 6439 7304 7602 2717
Size: 1569 903 1250 865 298 3429
Fragments arranged by size:
3429 1569 1250 903 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2225 2910 6977 882
Size: 617 726 685 4067 2219
Fragments arranged by size:
4067 2219 726 685 617
BsaAI yAC'GTr
Cuts at: 493 1204 2746 5110 6105 493
Size: 711 1542 2364 995 2702
Fragments arranged by size:
2702 2364 1542 995 711
BsaBI GATnn'nnATC
Cuts at: 3737 8308 3737
Size: 4571 3743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4491 6121 6503
Size: 53 83 186 936 2140 818 1630 382
Cuts at: 6503 7138 275
Size: 635 1451
Fragments arranged by size:
2140 1630 1451 936 818 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6747 7145 7419 7566 7977 6747
Size: 398 274 147 411 7084
Fragments arranged by size:
7084 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2388 2418 4241 4271 1178
Size: 30 165 30 985 30 1823 30 5221
Fragments arranged by size:
5221 1823 985 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2687 3053 3130 3965 3968 4943 5294
Size: 1150 351 366 77 835 3 975 351
Cuts at: 5294 5434 1186
Size: 140 4066
Fragments arranged by size:
4066 1150 975 835 366 351 351 140
77 3
BseYI C'CCAG_C
Cuts at: 2053 2084 2314 2677 2899 3120 3643 3754 4191
Size: 31 230 363 222 221 523 111 437
Cuts at: 4191 4823 4841 5215 7517 8013 2053
Size: 632 18 374 2302 496 2354
Fragments arranged by size:
2354 2302 632 523 496 437 374 363
230 222 221 111 31 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2747 3104 3598 4265 4334 4646 1163
Size: 573 1011 357 494 667 69 312 4831
Fragments arranged by size:
4831 1011 667 573 494 357 312 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 2626 5764 6525 7553 1540
Size: 275 186 14 611 3138 761 1028 2301
Fragments arranged by size:
3138 2301 1028 761 611 275 186 14
BsiHKAI G_wGCw'C
Cuts at: 2181 2960 3699 3909 4080 4090 4569 4833 4928
Size: 779 739 210 171 10 479 264 95
Cuts at: 4928 4945 5175 5289 6375 6460 7531 2181
Size: 17 230 114 1086 85 1071 2964
Fragments arranged by size:
2964 1086 1071 779 739 479 264 230
210 171 114 95 85 17 10
BsmI GAATG_Cn'
Cuts at: 5825 5918 5825
Size: 93 8221
BsmBI CGTCTCn'nnnn_
Cuts at: 4482 4482
Size: 8314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2209 2609 2797 2960 3333 3340
Size: 399 391 28 400 188 163 373 7
Cuts at: 3340 3503 3699 3879 3902 3909 3997 4080 4090
Size: 163 196 180 23 7 88 83 10
Cuts at: 4090 4166 4459 4569 4795 4833 4928 4945 5175
Size: 76 293 110 226 38 95 17 230
Cuts at: 5175 5289 5351 5547 5633 6375 6460 7531 1391
Size: 114 62 196 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 391 373 293
230 226 196 196 188 180 163 163
114 110 95 88 86 85 83 76
62 38 28 23 17 10 7 7
BspEI T'CCGG_A
Cuts at: 7977 7977
Size: 8314
BspHI T'CATG_A
Cuts at: 6203 7933 6203
Size: 1730 6584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2625 5157 5669 844
Size: 1781 2532 512 3489
Fragments arranged by size:
3489 2532 1781 512
BsrBI CCG'CTC
Cuts at: 1394 1823 2527 6201 1394
Size: 429 704 3674 3507
Fragments arranged by size:
3674 3507 704 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3780 4444 5210 6809 6983 65
Size: 1183 2532 664 766 1599 174 1396
Fragments arranged by size:
2532 1599 1396 1183 766 664 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4814 6958 7145 1700
Size: 110 2511 493 2144 187 2869
Fragments arranged by size:
2869 2511 2144 493 187 110
BsrGI T'GTAC_A
Cuts at: 96 5373 96
Size: 5277 3037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8314
BssSI C'ACGA_G
Cuts at: 2834 5315 6374 7386 2834
Size: 2481 1059 1012 3762
Fragments arranged by size:
3762 2481 1059 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8314
BstBI TT'CG_AA
Cuts at: 7118 7118
Size: 8314
BstEII G'GTnAC_C
Cuts at: 1184 3844 4228 1184
Size: 2660 384 5270
Fragments arranged by size:
5270 2660 384
BstXI CCAn_nnnn'nTGG
Cuts at: 3151 4072 5059 5215 5488 3151
Size: 921 987 156 273 5977
Fragments arranged by size:
5977 987 921 273 156
BstYI r'GATC_y
Cuts at: 1972 2076 2154 2653 3126 3738 5391 5644 6011
Size: 104 78 499 473 612 1653 253 367
Cuts at: 6011 6396 6413 7865 7951 7963 8309 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1653 1452 612 499 473 385 367
346 253 104 86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 3319 3319
Size: 8314
BtgI C'CryG_G
Cuts at: 513 1065 2854 3276 4680 5058 5308 5487 513
Size: 552 1789 422 1404 378 250 179 3340
Fragments arranged by size:
3340 1789 1404 552 422 378 250 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5009 487
Size: 683 56 3783 3792
Fragments arranged by size:
3792 3783 683 56
BtsI GCAGTG_nn'
Cuts at: 2424 2779 3676 3792 4606 5830 6624 6644 2424
Size: 355 897 116 814 1224 794 20 4094
Fragments arranged by size:
4094 1224 897 814 794 355 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8279
DraI TTT'AAA
Cuts at: 5618 5618
Size: 8314
DraIII CAC_nnn'GTG
Cuts at: 2792 3874 2792
Size: 1082 7232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7321 809
Size: 507 6005 1802
Fragments arranged by size:
6005 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2623 2910
Size: 54 1425 50 275 18 399 394 287
Cuts at: 2910 3534 3597 4323 4816 4902 5490 5761 6650
Size: 624 63 726 493 86 588 271 889
Cuts at: 6650 8279 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 726 624 588 493 399
394 287 275 271 86 63 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2623 5761 1537
Size: 275 811 3138 4090
Fragments arranged by size:
4090 3138 811 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2141 3914 4095 4574 4865 6244 1353
Size: 788 1773 181 479 291 1379 3423
Fragments arranged by size:
3423 1773 1379 788 479 291 181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2599 6901 7275 7421 387
Size: 1064 138 1010 4302 374 146 1280
Fragments arranged by size:
4302 1280 1064 1010 374 146 138
EcoICRI GAG'CTC
Cuts at: 3907 4567 3907
Size: 660 7654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2396 2690 3052 3198 3449 3694
Size: 393 482 282 294 362 146 251 245
Cuts at: 3694 3895 3939 4031 4276 4506 4739 6377 6974
Size: 201 44 92 245 230 233 1638 597
Cuts at: 6974 7761 8029 1239
Size: 787 268 1524
Fragments arranged by size:
1638 1524 787 597 482 393 362 294
282 268 251 245 245 233 230 201
146 92 44
EcoNI CCTnn'n_nnAGG
Cuts at: 3746 4214 3746
Size: 468 7846
EcoO109I rG'GnC_Cy
Cuts at: 2128 2205 2206 2681 2947 3100 3934 4455 4456
Size: 77 1 475 266 153 834 521 1
Cuts at: 4456 4791 2128
Size: 335 5651
Fragments arranged by size:
5651 834 521 475 335 266 153 77
1 1
EcoRI G'AATT_C
Cuts at: 5623 6001 5623
Size: 378 7936
EcoRV GAT'ATC
Cuts at: 2635 2635
Size: 8314
FseI GG_CCGG'CC
Cuts at: 4818 4818
Size: 8314
FspI TGC'GCA
Cuts at: 4807 6820 4807
Size: 2013 6301
HaeII r_GCGC'y
Cuts at: 1678 3079 3676 3688 4191 4291 4891 5279 7461
Size: 1401 597 12 503 100 600 388 2182
Cuts at: 7461 1678
Size: 2531
Fragments arranged by size:
2531 2182 1401 600 597 503 388 100
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2139 2171 2364 2396 5383
Size: 32 135 32 567 32 193 32 2987
Cuts at: 5383 5415 6955 6987 7029 7061 1373
Size: 32 1540 32 42 32 2626
Fragments arranged by size:
2987 2626 1540 567 193 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5658 5904 669
Size: 988 281 3720 246 3079
Fragments arranged by size:
3720 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8314
HpaI GTT'AAC
Cuts at: 5904 5904
Size: 8314
KasI G'GCGC_C
Cuts at: 3672 3672
Size: 8314
KpnI G_GTAC'C
Cuts at: 5639 5639
Size: 8314
MfeI C'AATT_G
Cuts at: 5913 5913
Size: 8314
MluI A'CGCG_T
Cuts at: 8190 8190
Size: 8314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7428 7612 1305
Size: 6123 184 2007
Fragments arranged by size:
6123 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2231 2912 5492 10
Size: 54 1425 343 399 681 2580 2832
Fragments arranged by size:
2832 2580 1425 681 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2769 4121 5057 5486 6272 6631 6790
Size: 648 1603 1352 936 429 786 359 159
Cuts at: 6790 518
Size: 2042
Fragments arranged by size:
2042 1603 1352 936 786 648 429 359
159
MspA1I CmG'CkG
Cuts at: 1606 2665 2986 3073 3088 3999 4519 4845 5730
Size: 1059 321 87 15 911 520 326 885
Cuts at: 5730 6407 7555 7800 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1059 911 885 677 520 326
321 245 87 15
NaeI GCC'GGC
Cuts at: 4816 4816
Size: 8314
NarI GG'CG_CC
Cuts at: 3673 3673
Size: 8314
NcoI C'CATG_G
Cuts at: 513 1065 2854 5308 513
Size: 552 1789 2454 3519
Fragments arranged by size:
3519 2454 1789 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8314
NgoMIV G'CCGG_C
Cuts at: 4814 4814
Size: 8314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8314
NotI GC'GGCC_GC
Cuts at: 5761 5761
Size: 8314
NspI r_CATG'y
Cuts at: 1132 1229 3170 3434 3842 4058 4178 4806 5672
Size: 97 1941 264 408 216 120 628 866
Cuts at: 5672 7217 1132
Size: 1545 2229
Fragments arranged by size:
2229 1941 1545 866 628 408 264 216
120 97
PasI CC'CwG_GG
Cuts at: 1348 3268 4234 4609 5134 1348
Size: 1920 966 375 525 4528
Fragments arranged by size:
4528 1920 966 525 375
PciI A'CATG_T
Cuts at: 7213 7213
Size: 8314
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3754 3841 5609 1108
Size: 2646 87 1768 3813
Fragments arranged by size:
3813 2646 1768 87
PfoI T'CCnGG_A
Cuts at: 2706 3465 2706
Size: 759 7555
PmeI GTTT'AAAC
Cuts at: 5618 5618
Size: 8314
PmlI CAC'GTG
Cuts at: 5110 5110
Size: 8314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6383 6415 7896 7928 6383
Size: 32 1481 32 6769
Fragments arranged by size:
6769 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2681 3934 2681
Size: 1253 7061
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8314
PsiI TTA'TAA
Cuts at: 5884 5884
Size: 8314
PspOMI G'GGCC_C
Cuts at: 2205 4455 4791 5347 2205
Size: 2250 336 556 5172
Fragments arranged by size:
5172 2250 556 336
PstI C_TGCA'G
Cuts at: 830 2955 3492 4768 5666 830
Size: 2125 537 1276 898 3478
Fragments arranged by size:
3478 2125 1276 898 537
PvuI CG_AT'CG
Cuts at: 2001 2015 2001
Size: 14 8300
PvuII CAG'CTG
Cuts at: 2665 2986 3088 4519 2665
Size: 321 102 1431 6460
Fragments arranged by size:
6460 1431 321 102
SacI G_AGCT'C
Cuts at: 3909 4569 3909
Size: 660 7654
SalI G'TCGA_C
Cuts at: 1936 5656 1936
Size: 3720 4594
SanDI GG'GwC_CC
Cuts at: 2681 2681
Size: 8314
SapI GCTCTTCn'nnn_
Cuts at: 2141 3914 4095 4574 2141
Size: 1773 181 479 5881
Fragments arranged by size:
5881 1773 479 181
SbfI CC_TGCA'GG
Cuts at: 5666 5666
Size: 8314
ScaI AGT'ACT
Cuts at: 6562 8274 6562
Size: 1712 6602
SexAI A'CCwGG_T
Cuts at: 3870 5596 3870
Size: 1726 6588
SfcI C'TryA_G
Cuts at: 826 1045 2951 3488 3590 4370 4764 5079 5662
Size: 219 1906 537 102 780 394 315 583
Cuts at: 5662 6797 7478 7669 826
Size: 1135 681 191 1471
Fragments arranged by size:
1906 1471 1135 780 681 583 537 394
315 219 191 102
SfiI GGCCn_nnn'nGGCC
Cuts at: 2204 3098 2204
Size: 894 7420
SfoI GGC'GCC
Cuts at: 3674 3674
Size: 8314
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8314
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8314
SmaI CCC'GGG
Cuts at: 1872 4796 5641 1872
Size: 2924 845 4545
Fragments arranged by size:
4545 2924 845
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 4301 5168 6418 7319 7581
Size: 197 926 753 1605 867 1250 901 262
Cuts at: 7581 820
Size: 1553
Fragments arranged by size:
1605 1553 1250 926 901 867 753 262
197
SnaBI TAC'GTA
Cuts at: 493 6105 493
Size: 5612 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8314
SphI G_CATG'C
Cuts at: 1132 3170 3434 4178 4806 5672 1132
Size: 2038 264 744 628 866 3774
Fragments arranged by size:
3774 2038 866 744 628 264
SrfI GCCC'GGGC
Cuts at: 4796 4796
Size: 8314
SspI AAT'ATT
Cuts at: 5 52 6238 5
Size: 47 6186 2081
Fragments arranged by size:
6186 2081 47
StuI AGG'CCT
Cuts at: 1517 2741 3324 1517
Size: 1224 583 6507
Fragments arranged by size:
6507 1224 583
StyI C'CwwG_G
Cuts at: 513 1065 2304 2599 2854 2923 3325 4119 4167
Size: 552 1239 295 255 69 402 794 48
Cuts at: 4167 4716 5308 5549 5753 513
Size: 549 592 241 204 3074
Fragments arranged by size:
3074 1239 794 592 552 549 402 295
255 241 204 69 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4343 4582 5147 5207 5514 6341 6358
Size: 1255 2763 239 565 60 307 827 17
Cuts at: 6358 6511 6696 7141 325
Size: 153 185 445 1498
Fragments arranged by size:
2763 1498 1255 827 565 445 307 239
185 153 60 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5373 6560 8272
Size: 274 80 33 51 1443 3396 1187 1712
Cuts at: 8272 96
Size: 138
Fragments arranged by size:
3396 1712 1443 1187 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2061 2093 2388 2420 2624 2656 3390 3422 8274
Size: 32 295 32 204 32 734 32 4852
Cuts at: 8274 8306 2061
Size: 32 2069
Fragments arranged by size:
4852 2069 734 295 204 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5598 1316
Size: 4282 4032
XbaI T'CTAG_A
Cuts at: 5650 5650
Size: 8314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2081 2165 2311 3529 3655 4531 4653 2081
Size: 84 146 1218 126 876 122 5742
Fragments arranged by size:
5742 1218 876 146 126 122 84
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8314
XmaI C'CCGG_G
Cuts at: 1870 4794 5639 1870
Size: 2924 845 4545
Fragments arranged by size:
4545 2924 845
XmnI GAAnn'nnTTC
Cuts at: 6443 6443
Size: 8314
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6122 276
Size: 53 83 186 936 4588 2468
Fragments arranged by size:
4588 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BfrBI BsiWI BstZ17I FalI
FspAI NruI NsiI PacI PspXI PsrI RsrII SacII
SwaI