Restriction map for
pFN21AE0075
(Circular) (Six-base) MAPSORT of: pFN21AE0075.seq Check: 7576 from: 1 to: 9046
pFN21AE0075 9046 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3315 6856 278
Size: 53 83 186 936 1779 3541 2468
Fragments arranged by size:
3541 2468 1779 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2574 6389 1937
Size: 637 3815 4594
Fragments arranged by size:
4594 3815 637
Acc65I G'GTAC_C
Cuts at: 6367 6367
Size: 9046
AclI AA'CG_TT
Cuts at: 1563 6345 7173 7546 1563
Size: 4782 828 373 3063
Fragments arranged by size:
4782 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3553 3647 4531 4544 4553 6006 7109 8493
Size: 1921 94 884 13 9 1453 1103 1384
Cuts at: 8493 8761 1632
Size: 268 1917
Fragments arranged by size:
1921 1917 1453 1384 1103 884 268 94
13 9
AfeI AGC'GCT
Cuts at: 2526 4756 2526
Size: 2230 6816
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8849
AflIII A'CryG_T
Cuts at: 1203 2577 4023 4410 5367 7945 8922 1203
Size: 1374 1446 387 957 2578 977 1327
Fragments arranged by size:
2578 1446 1374 1327 977 957 387
AgeI A'CCGG_T
Cuts at: 7877 7877
Size: 9046
AhdI GACnn_n'nnGTC
Cuts at: 5875 7775 5875
Size: 1900 7146
AleI CACnn'nnGTG
Cuts at: 1166 4022 4313 6113 1166
Size: 2856 291 1800 4099
Fragments arranged by size:
4099 2856 1800 291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5366 5388 6101 1184
Size: 4182 22 713 4129
Fragments arranged by size:
4182 4129 713 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6047 6079 6047
Size: 32 9014
AlwNI CAG_nnn'CTG
Cuts at: 2061 2169 3449 4554 4590 4723 6789 8361 2061
Size: 108 1280 1105 36 133 2066 1572 2746
Fragments arranged by size:
2746 2066 1572 1280 1105 133 108 36
ApaI G_GGCC'C
Cuts at: 6016 6026 6037 6016
Size: 10 11 9025
Fragments arranged by size:
9025 11 10
ApaLI G'TGCA_C
Cuts at: 6146 7103 8259 6146
Size: 957 1156 6933
Fragments arranged by size:
6933 1156 957
ApoI r'AATT_y
Cuts at: 1493 2792 2938 3480 5976 6355 6581 6733 8842
Size: 1299 146 542 2496 379 226 152 2109
Cuts at: 8842 1493
Size: 1697
Fragments arranged by size:
2496 2109 1697 1299 542 379 226 152
146
AseI AT'TA_AT
Cuts at: 160 1032 4746 7600 160
Size: 872 3714 2854 1606
Fragments arranged by size:
3714 2854 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9046
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2366 4172 4311 4350 5848 6183
Size: 73 131 292 1806 139 39 1498 335
Cuts at: 6183 6327 6371 1870
Size: 144 44 4545
Fragments arranged by size:
4545 1806 1498 335 292 144 139 131
73 44 39
AvrII C'CTAG_G
Cuts at: 2749 2749
Size: 9046
BamHI G'GATC_C
Cuts at: 6376 6743 6376
Size: 367 8679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 3404 4304 5390 6367
Size: 325 217 627 282 1335 900 1086 977
Cuts at: 6367 7822 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1335 1086 977 900 627 325
282 217
BanII G_rGCy'C
Cuts at: 1391 2034 4109 4179 4908 6016 6026 6037 6365
Size: 643 2075 70 729 1108 10 11 328
Cuts at: 6365 1391
Size: 4072
Fragments arranged by size:
4072 2075 1108 729 643 328 70 11
10
BbeI G_GCGC'C
Cuts at: 5394 5394
Size: 9046
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5131 5785 928
Size: 971 3232 654 4189
Fragments arranged by size:
4189 3232 971 654
BbvCI CC'TCA_GC
Cuts at: 2859 4094 5705 2859
Size: 1235 1611 6200
Fragments arranged by size:
6200 1611 1235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5107 5141 7237 7271 1903
Size: 34 3170 34 2096 34 3678
Fragments arranged by size:
3678 3170 2096 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6938 8148 6938
Size: 1210 7836
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9046
BfrBI ATG'CAT
Cuts at: 4577 4577
Size: 9046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2236 3647 4302
Size: 107 122 71 631 605 564 1411 655
Cuts at: 4302 4817 7657 136
Size: 515 2840 1525
Fragments arranged by size:
2840 1525 1411 655 631 605 564 515
122 107 71
BglII A'GATC_T
Cuts at: 4056 9041 4056
Size: 4985 4061
BlpI GC'TnA_GC
Cuts at: 4041 6463 4041
Size: 2422 6624
Bme1580I G_kGCm'C
Cuts at: 1790 2074 5250 6016 6026 6037 6090 6150 7107
Size: 284 3176 766 10 11 53 60 957
Cuts at: 7107 8263 1790
Size: 1156 2573
Fragments arranged by size:
3176 2573 1156 957 766 284 60 53
11 10
BmgBI CAC'GTC
Cuts at: 1318 3657 6256 6307 1318
Size: 2339 2599 51 4057
Fragments arranged by size:
4057 2599 2339 51
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2282 3467 4138
Size: 416 53 83 399 24 865 1185 671
Cuts at: 4138 4600 4915 6777 7735 442
Size: 462 315 1862 958 1753
Fragments arranged by size:
1862 1753 1185 958 865 671 462 416
399 315 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9046
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3166 4066 6209 3166
Size: 900 2143 6003
Fragments arranged by size:
6003 2143 900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 2210 2537 3212 3713 4141 5381 5544
Size: 870 101 327 675 501 428 1240 163
Cuts at: 5544 7706 1239
Size: 2162 2579
Fragments arranged by size:
2579 2162 1240 870 675 501 428 327
163 101
Bpu10I CC'TnA_GC
Cuts at: 2859 4094 5598 5705 2859
Size: 1235 1504 107 6200
Fragments arranged by size:
6200 1504 1235 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7171 8036 8334 7171
Size: 865 298 7883
Fragments arranged by size:
7883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2363 7709 882
Size: 617 864 5346 2219
Fragments arranged by size:
5346 2219 864 617
BsaAI yAC'GTr
Cuts at: 493 1204 4024 6244 6837 493
Size: 711 2820 2220 593 2702
Fragments arranged by size:
2820 2702 2220 711 593
BsaBI GATnn'nnATC
Cuts at: 3804 9040 3804
Size: 5236 3810
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 3312 3423 5391
Size: 53 83 186 936 642 1137 111 1968
Cuts at: 5391 6853 7235 7870 275
Size: 1462 382 635 1451
Fragments arranged by size:
1968 1462 1451 1137 936 642 635 382
186 111 83 53
BsaWI w'CCGG_w
Cuts at: 6258 7479 7877 8151 8298 8709 6258
Size: 1221 398 274 147 411 6595
Fragments arranged by size:
6595 1221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2372 2394 2402 2424 3630
Size: 30 165 30 969 22 8 22 1206
Cuts at: 3630 3660 4124 4154 4468 4498 5354 5384 1178
Size: 30 464 30 314 30 856 30 4840
Fragments arranged by size:
4840 1206 969 856 464 314 165 30
30 30 30 30 30 22 22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 2396 3035 5726 6283 6332 1186
Size: 1126 84 639 2691 557 49 3900
Fragments arranged by size:
3900 2691 1126 639 557 84 49
BseYI C'CCAG_C
Cuts at: 2162 2835 3381 4285 4751 5407 5609 5733 6018
Size: 673 546 904 466 656 202 124 285
Cuts at: 6018 6058 8249 8745 2162
Size: 40 2191 496 2463
Fragments arranged by size:
2463 2191 904 673 656 546 496 466
285 202 124 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 4085 4365 4523 5101 1163
Size: 573 402 1947 280 158 578 5108
Fragments arranged by size:
5108 1947 578 573 402 280 158
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2084 2904 5163 5199 6496 7257
Size: 275 186 83 820 2259 36 1297 761
Cuts at: 7257 8285 1540
Size: 1028 2301
Fragments arranged by size:
2301 2259 1297 1028 820 761 275 186
83 36
BsiHKAI G_wGCw'C
Cuts at: 3129 3470 4118 5367 6150 7107 7192 8263 3129
Size: 341 648 1249 783 957 85 1071 3912
Fragments arranged by size:
3912 1249 1071 957 783 648 341 85
BsmI GAATG_Cn'
Cuts at: 5992 6317 6557 6650 5992
Size: 325 240 93 8388
Fragments arranged by size:
8388 325 240 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4314 5958 4314
Size: 1644 7402
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2074 3129 3470 4109 4118 4179
Size: 399 244 40 1055 341 639 9 61
Cuts at: 4179 4908 5250 5367 6016 6026 6037 6090 6150
Size: 729 342 117 649 10 11 53 60
Cuts at: 6150 6365 7107 7192 8263 1391
Size: 215 742 85 1071 2174
Fragments arranged by size:
2174 1071 1055 742 729 649 639 399
342 341 244 215 117 85 61 60
53 40 11 10 9
BspEI T'CCGG_A
Cuts at: 8709 8709
Size: 9046
BspHI T'CATG_A
Cuts at: 2475 6935 8665 2475
Size: 4460 1730 2856
Fragments arranged by size:
4460 2856 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4143 6401 844
Size: 3299 2258 3489
Fragments arranged by size:
3489 3299 2258
BsrBI CCG'CTC
Cuts at: 1394 1823 3710 6933 1394
Size: 429 1887 3223 3507
Fragments arranged by size:
3507 3223 1887 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3673 7541 7715 65
Size: 1183 2425 3868 174 1396
Fragments arranged by size:
3868 2425 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2083 2157 4673 7690 7877 1700
Size: 110 273 74 2516 3017 187 2869
Fragments arranged by size:
3017 2869 2516 273 187 110 74
BsrGI T'GTAC_A
Cuts at: 96 2580 96
Size: 2484 6562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9046
BssSI C'ACGA_G
Cuts at: 5126 7106 8118 5126
Size: 1980 1012 6054
Fragments arranged by size:
6054 1980 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9046
BstBI TT'CG_AA
Cuts at: 6278 7850 6278
Size: 1572 7474
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9046
BstXI CCAn_nnnn'nTGG
Cuts at: 2444 3283 4258 5407 6073 2444
Size: 839 975 1149 666 5417
Fragments arranged by size:
5417 1149 975 839 666
BstYI r'GATC_y
Cuts at: 1972 4056 5429 6376 6743 7128 7145 8597 8683
Size: 2084 1373 947 367 385 17 1452 86
Cuts at: 8683 8695 9041 1972
Size: 12 346 1977
Fragments arranged by size:
2084 1977 1452 1373 947 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2575 2575
Size: 9046
Bsu36I CC'TnA_GG
Cuts at: 3885 5550 3885
Size: 1665 7381
BtgI C'CryG_G
Cuts at: 513 1065 2004 2041 3408 3762 5250 6072 6220
Size: 552 939 37 1367 354 1488 822 148
Cuts at: 6220 513
Size: 3339
Fragments arranged by size:
3339 1488 1367 939 822 552 354 148
37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8307
Fragments arranged by size:
8307 683 56
BtsI GCAGTG_nn'
Cuts at: 2597 4165 6066 6562 7356 7376 2597
Size: 1568 1901 496 794 20 4267
Fragments arranged by size:
4267 1901 1568 794 496 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9011
DraI TTT'AAA
Cuts at: 2269 6350 2269
Size: 4081 4965
DraIII CAC_nnn'GTG
Cuts at: 5764 5764
Size: 9046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2412 3317 4643 5447 5695 8053 809
Size: 507 1096 905 1326 804 248 2358 1802
Fragments arranged by size:
2358 1802 1326 1096 905 804 507 248
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2081 2218 2901
Size: 54 1425 50 275 18 251 137 683
Cuts at: 2901 3330 3687 4829 5160 5322 5638 5939 6493
Size: 429 357 1142 331 162 316 301 554
Cuts at: 6493 7382 9011 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1142 889 683 554 429 357
331 316 301 275 251 162 137 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2081 2901 5160 6493 1537
Size: 275 269 820 2259 1333 4090
Fragments arranged by size:
4090 2259 1333 820 275 269
EarI CTCTTCn'nnn_
Cuts at: 1353 2445 2796 5057 6976 1353
Size: 1092 351 2261 1919 3423
Fragments arranged by size:
3423 2261 1919 1092 351
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5066 6190 7633 8007 8153 387
Size: 1064 138 3477 1124 1443 374 146 1280
Fragments arranged by size:
3477 1443 1280 1124 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2210 2537 3212 3553 3647 3713
Size: 393 477 101 327 675 341 94 66
Cuts at: 3713 4141 4531 4544 4553 5381 5544 6006 7109
Size: 428 390 13 9 828 163 462 1103
Cuts at: 7109 7706 8493 8761 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1103 828 787 675 597 477 462
428 393 390 341 327 268 163 101
94 66 13 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3202 5708 6230 3202
Size: 2506 522 6018
Fragments arranged by size:
6018 2506 522
EcoO109I rG'GnC_Cy
Cuts at: 2334 4132 5750 5877 5917 6012 6022 6023 6033
Size: 1798 1618 127 40 95 10 1 10
Cuts at: 6033 6235 6324 2334
Size: 202 89 5056
Fragments arranged by size:
5056 1798 1618 202 127 95 89 40
10 10 1
EcoRI G'AATT_C
Cuts at: 2792 6355 6733 2792
Size: 3563 378 5105
Fragments arranged by size:
5105 3563 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3615 3615
Size: 9046
FspI TGC'GCA
Cuts at: 7552 7552
Size: 9046
HaeII r_GCGC'y
Cuts at: 1678 2231 2528 3075 4758 5189 5394 8193 1678
Size: 553 297 547 1683 431 205 2799 2531
Fragments arranged by size:
2799 2531 1683 553 547 431 297 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2616 2648 4124 4156 5861
Size: 32 135 32 1044 32 1476 32 1705
Cuts at: 5861 5893 7687 7719 7761 7793 1373
Size: 32 1794 32 42 32 2626
Fragments arranged by size:
2626 1794 1705 1476 1044 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4710 6390 6636 669
Size: 988 281 2772 1680 246 3079
Fragments arranged by size:
3079 2772 1680 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9046
HpaI GTT'AAC
Cuts at: 6636 6636
Size: 9046
KasI G'GCGC_C
Cuts at: 5390 5390
Size: 9046
KpnI G_GTAC'C
Cuts at: 6371 6371
Size: 9046
MfeI C'AATT_G
Cuts at: 4974 6645 4974
Size: 1671 7375
MluI A'CGCG_T
Cuts at: 8922 8922
Size: 9046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2380 4176 4485 5691 6366 8160 8344 1305
Size: 1075 1796 309 1206 675 1794 184 2007
Fragments arranged by size:
2007 1796 1794 1206 1075 675 309 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3332 5941 10
Size: 54 1425 343 1500 2609 3115
Fragments arranged by size:
3115 2609 1500 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3231 3326 4022 4313 5102 5174 5282
Size: 648 2065 95 696 291 789 72 108
Cuts at: 5282 6071 6113 7004 7363 7522 518
Size: 789 42 891 359 159 2042
Fragments arranged by size:
2065 2042 891 789 789 696 648 359
291 159 108 95 72 42
MspA1I CmG'CkG
Cuts at: 1606 2043 2058 2233 2835 3336 3737 4040 4098
Size: 437 15 175 602 501 401 303 58
Cuts at: 4098 5652 6018 6462 7139 8287 8532 1606
Size: 1554 366 444 677 1148 245 2120
Fragments arranged by size:
2120 1554 1148 677 602 501 444 437
401 366 303 245 175 58 15
NaeI GCC'GGC
Cuts at: 2085 2085
Size: 9046
NarI GG'CG_CC
Cuts at: 5391 5391
Size: 9046
NcoI C'CATG_G
Cuts at: 513 1065 2004 3762 6072 513
Size: 552 939 1758 2310 3487
Fragments arranged by size:
3487 2310 1758 939 552
NdeI CA'TA_TG
Cuts at: 387 3211 387
Size: 2824 6222
NgoMIV G'CCGG_C
Cuts at: 2083 2083
Size: 9046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9046
NotI GC'GGCC_GC
Cuts at: 6493 6493
Size: 9046
NsiI A_TGCA'T
Cuts at: 4579 4579
Size: 9046
NspI r_CATG'y
Cuts at: 1132 1229 2581 4414 4873 5371 6404 7949 1132
Size: 97 1352 1833 459 498 1033 1545 2229
Fragments arranged by size:
2229 1833 1545 1352 1033 498 459 97
PasI CC'CwG_GG
Cuts at: 1348 3400 6164 1348
Size: 2052 2764 4230
Fragments arranged by size:
4230 2764 2052
PciI A'CATG_T
Cuts at: 2577 4410 5367 7945 2577
Size: 1833 957 2578 3678
Fragments arranged by size:
3678 2578 1833 957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4533 1108
Size: 3425 5621
PfoI T'CCnGG_A
Cuts at: 2796 3189 5382 5887 2796
Size: 393 2193 505 5955
Fragments arranged by size:
5955 2193 505 393
PmeI GTTT'AAAC
Cuts at: 6350 6350
Size: 9046
PmlI CAC'GTG
Cuts at: 4024 6244 4024
Size: 2220 6826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7115 7147 8628 8660 7115
Size: 32 1481 32 7501
Fragments arranged by size:
7501 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4132 5877 5917 6235 4132
Size: 1745 40 318 6943
Fragments arranged by size:
6943 1745 318 40
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9046
PsiI TTA'TAA
Cuts at: 6616 6616
Size: 9046
PspOMI G'GGCC_C
Cuts at: 6012 6022 6033 6012
Size: 10 11 9025
Fragments arranged by size:
9025 11 10
PspXI vC'TCGA_Gb
Cuts at: 4172 4172
Size: 9046
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4907 4939 4907
Size: 32 9014
PstI C_TGCA'G
Cuts at: 830 3087 3454 5000 6303 6398 830
Size: 2257 367 1546 1303 95 3478
Fragments arranged by size:
3478 2257 1546 1303 367 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9046
PvuII CAG'CTG
Cuts at: 2835 4098 2835
Size: 1263 7783
RsrII CG'GwC_CG
Cuts at: 2140 2140
Size: 9046
SacII CC_GC'GG
Cuts at: 2044 2044
Size: 9046
SalI G'TCGA_C
Cuts at: 1936 6388 1936
Size: 4452 4594
SanDI GG'GwC_CC
Cuts at: 5877 5917 5877
Size: 40 9006
SbfI CC_TGCA'GG
Cuts at: 6398 6398
Size: 9046
ScaI AGT'ACT
Cuts at: 7294 9006 7294
Size: 1712 7334
SfcI C'TryA_G
Cuts at: 826 1045 3083 3450 3919 3937 4996 5950 6299
Size: 219 2038 367 469 18 1059 954 349
Cuts at: 6299 6394 7529 8210 8401 826
Size: 95 1135 681 191 1471
Fragments arranged by size:
2038 1471 1135 1059 954 681 469 367
349 219 191 95 18
SfoI GGC'GCC
Cuts at: 5392 5392
Size: 9046
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9046
SmaI CCC'GGG
Cuts at: 1872 2076 6329 6373 1872
Size: 204 4253 44 4545
Fragments arranged by size:
4545 4253 204 44
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 4172 7150 8051 8313 820
Size: 197 926 423 1806 2978 901 262 1553
Fragments arranged by size:
2978 1806 1553 926 901 423 262 197
SnaBI TAC'GTA
Cuts at: 493 6837 493
Size: 6344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9046
SphI G_CATG'C
Cuts at: 1132 6404 1132
Size: 5272 3774
SspI AAT'ATT
Cuts at: 5 52 6970 5
Size: 47 6918 2081
Fragments arranged by size:
6918 2081 47
StuI AGG'CCT
Cuts at: 1517 3197 3890 5703 1517
Size: 1680 693 1813 4860
Fragments arranged by size:
4860 1813 1680 693
StyI C'CwwG_G
Cuts at: 513 1065 2004 2202 2749 3762 4251 6072 6485
Size: 552 939 198 547 1013 489 1821 413
Cuts at: 6485 513
Size: 3074
Fragments arranged by size:
3074 1821 1013 939 552 547 489 413
198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2380 3023 4439 5850 7073 7090 7243
Size: 1255 800 643 1416 1411 1223 17 153
Cuts at: 7243 7428 7873 325
Size: 185 445 1498
Fragments arranged by size:
1498 1416 1411 1255 1223 800 643 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 4026 5064
Size: 274 80 33 51 1443 603 1446 1038
Cuts at: 5064 7292 9004 96
Size: 2228 1712 138
Fragments arranged by size:
2228 1712 1446 1443 1038 603 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 2594 2626 3630 3662 9006 9038 2372
Size: 32 190 32 1004 32 5344 32 2380
Fragments arranged by size:
5344 2380 1004 190 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4796 5695 1316
Size: 3480 899 4667
Fragments arranged by size:
4667 3480 899
XbaI T'CTAG_A
Cuts at: 6382 6382
Size: 9046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3280 3280
Size: 9046
XhoI C'TCGA_G
Cuts at: 1943 2366 4172 1943
Size: 423 1806 6817
Fragments arranged by size:
6817 1806 423
XmaI C'CCGG_G
Cuts at: 1870 2074 6327 6371 1870
Size: 204 4253 44 4545
Fragments arranged by size:
4545 4253 204 44
XmnI GAAnn'nnTTC
Cuts at: 3302 7175 3302
Size: 3873 5173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3313 6854 276
Size: 53 83 186 936 1779 3541 2468
Fragments arranged by size:
3541 2468 1779 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BsiWI EcoICRI EcoRV FseI FspAI
NruI PacI SacI SapI SexAI SfiI SrfI SwaI