Restriction map for
pFN21AE0073
(Circular) (Six-base) MAPSORT of: pFN21AE0073.seq Check: 1043 from: 1 to: 8665
pFN21AE0073 8665 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6475 278
Size: 53 83 186 936 4939 2468
Fragments arranged by size:
4939 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2688 6008 1937
Size: 751 3320 4594
Fragments arranged by size:
4594 3320 751
Acc65I G'GTAC_C
Cuts at: 5986 5986
Size: 8665
AclI AA'CG_TT
Cuts at: 1563 6792 7165 1563
Size: 5229 373 3063
Fragments arranged by size:
5229 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5456 5555 6728 8112 8380 1632
Size: 3824 99 1173 1384 268 1917
Fragments arranged by size:
3824 1917 1384 1173 268 99
AfeI AGC'GCT
Cuts at: 4174 5677 4174
Size: 1503 7162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8468
AflIII A'CryG_T
Cuts at: 1203 2739 7564 8541 1203
Size: 1536 4825 977 1327
Fragments arranged by size:
4825 1536 1327 977
AgeI A'CCGG_T
Cuts at: 7496 7496
Size: 8665
AhdI GACnn_n'nnGTC
Cuts at: 3562 5179 7394 3562
Size: 1617 2215 4833
Fragments arranged by size:
4833 2215 1617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5694 5852 1184
Size: 4510 158 3997
Fragments arranged by size:
4510 3997 158
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4445 4477 4445
Size: 32 8633
AlwNI CAG_nnn'CTG
Cuts at: 3328 3568 3712 5527 5610 5845 6408 7980 3328
Size: 240 144 1815 83 235 563 1572 4013
Fragments arranged by size:
4013 1815 1572 563 240 235 144 83
ApaI G_GGCC'C
Cuts at: 2136 4040 4528 4666 5375 2136
Size: 1904 488 138 709 5426
Fragments arranged by size:
5426 1904 709 488 138
ApaLI G'TGCA_C
Cuts at: 2267 6722 7878 2267
Size: 4455 1156 3054
Fragments arranged by size:
4455 3054 1156
ApoI r'AATT_y
Cuts at: 1493 3118 5974 6200 6352 8461 1493
Size: 1625 2856 226 152 2109 1697
Fragments arranged by size:
2856 2109 1697 1625 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7219 160
Size: 872 6187 1606
Fragments arranged by size:
6187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8665
AvaI C'yCGr_G
Cuts at: 1870 1943 2308 2372 2710 2725 2815 3098 3746
Size: 73 365 64 338 15 90 283 648
Cuts at: 3746 3952 4018 4148 4223 4520 4624 4714 5374
Size: 206 66 130 75 297 104 90 660
Cuts at: 5374 5886 5990 1870
Size: 512 104 4545
Fragments arranged by size:
4545 660 648 512 365 338 297 283
206 130 104 104 90 90 75 73
66 64 15
BamHI G'GATC_C
Cuts at: 2149 5995 6362 2149
Size: 3846 367 4452
Fragments arranged by size:
4452 3846 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 2719 2819 3006 3313
Size: 325 217 627 756 176 100 187 307
Cuts at: 3313 3836 3851 3868 3994 4352 4748 4871 4993
Size: 523 15 17 126 358 396 123 122
Cuts at: 4993 5273 5296 5432 5740 5986 7441 618
Size: 280 23 136 308 246 1455 1842
Fragments arranged by size:
1842 1455 756 627 523 396 358 325
308 307 280 246 217 187 176 136
126 123 122 100 23 17 15
BanII G_rGCy'C
Cuts at: 1391 2031 2136 2205 2594 2991 3002 4040 4167
Size: 640 105 69 389 397 11 1038 127
Cuts at: 4167 4430 4528 4631 4666 4868 4931 5375 5984
Size: 263 98 103 35 202 63 444 609
Cuts at: 5984 1391
Size: 4072
Fragments arranged by size:
4072 1038 640 609 444 397 389 263
202 127 105 103 98 69 63 35
11
BbeI G_GCGC'C
Cuts at: 2723 2823 3317 3840 3872 3998 5277 5300 5436
Size: 100 494 523 32 126 1279 23 136
Cuts at: 5436 5744 2723
Size: 308 5644
Fragments arranged by size:
5644 1279 523 494 308 136 126 100
32 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7694
BbvCI CC'TCA_GC
Cuts at: 3048 5812 3048
Size: 2764 5901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2378 2412 4042 4076 5780 5814 6856
Size: 34 441 34 1630 34 1704 34 1042
Cuts at: 6856 6890 1903
Size: 34 3678
Fragments arranged by size:
3678 1704 1630 1042 441 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3240 6557 7767 3240
Size: 3317 1210 4138
Fragments arranged by size:
4138 3317 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2406 2564 3100
Size: 107 122 71 631 605 734 158 536
Cuts at: 3100 3489 3765 3789 4236 5385 5530 5567 5576
Size: 389 276 24 447 1149 145 37 9
Cuts at: 5576 5905 7276 136
Size: 329 1371 1525
Fragments arranged by size:
1525 1371 1149 734 631 605 536 447
389 329 276 158 145 122 107 71
37 24 9
BglII A'GATC_T
Cuts at: 8660 8660
Size: 8665
BlpI GC'TnA_GC
Cuts at: 3732 6082 3732
Size: 2350 6315
Bme1580I G_kGCm'C
Cuts at: 1790 2136 2271 3128 3167 3513 3854 4040 4083
Size: 346 135 857 39 346 341 186 43
Cuts at: 4083 4528 4666 4996 5375 5390 5901 6726 7882
Size: 445 138 330 379 15 511 825 1156
Cuts at: 7882 1790
Size: 2573
Fragments arranged by size:
2573 1156 857 825 511 445 379 346
346 341 330 186 138 135 43 39
15
BmgBI CAC'GTC
Cuts at: 1318 3754 4785 5022 5869 1318
Size: 2436 1031 237 847 4114
Fragments arranged by size:
4114 2436 1031 847 237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2136 6396 7354
Size: 416 53 83 399 24 719 4260 958
Cuts at: 7354 442
Size: 1753
Fragments arranged by size:
4260 1753 958 719 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8665
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 5155 2740
Size: 2415 6250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2533 2614 2675 2986 3005 3266 4716 5420
Size: 1294 81 61 311 19 261 1450 704
Cuts at: 5420 5483 7325 1239
Size: 63 1842 2579
Fragments arranged by size:
2579 1842 1450 1294 704 311 261 81
63 61 19
Bpu10I CC'TnA_GC
Cuts at: 3048 5812 3048
Size: 2764 5901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6790 7655 7953 6790
Size: 865 298 7502
Fragments arranged by size:
7502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2072 3918 4589 4813 7328 882
Size: 617 573 1846 671 224 2515 2219
Fragments arranged by size:
2515 2219 1846 671 617 573 224
BsaAI yAC'GTr
Cuts at: 493 1204 4078 4861 6456 493
Size: 711 2874 783 1595 2702
Fragments arranged by size:
2874 2702 1595 783 711
BsaBI GATnn'nnATC
Cuts at: 8659 8659
Size: 8665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2720 2820 3314 3689
Size: 53 83 186 936 1187 100 494 375
Cuts at: 3689 3837 3844 3869 3995 5000 5274 5297 5433
Size: 148 7 25 126 1005 274 23 136
Cuts at: 5433 5741 5877 6472 6854 7489 275
Size: 308 136 595 382 635 1451
Fragments arranged by size:
1451 1187 1005 936 635 595 494 382
375 308 274 186 148 136 136 126
100 83 53 25 23 7
BsaWI w'CCGG_w
Cuts at: 2081 3252 4900 7098 7496 7770 7917 8328 2081
Size: 1171 1648 2198 398 274 147 411 2418
Fragments arranged by size:
2418 2198 1648 1171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8440
Fragments arranged by size:
8440 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3291 3993 1186
Size: 2105 702 5858
Fragments arranged by size:
5858 2105 702
BseYI C'CCAG_C
Cuts at: 2167 2874 2895 2913 3027 3147 3170 3206 3801
Size: 707 21 18 114 120 23 36 595
Cuts at: 3801 3986 4169 4304 4382 4423 4442 5599 5820
Size: 185 183 135 78 41 19 1157 221
Cuts at: 5820 7868 8364 2167
Size: 2048 496 2468
Fragments arranged by size:
2468 2048 1157 707 595 496 221 185
183 135 120 114 78 41 36 23
21 19 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 4272 5842 1163
Size: 573 636 1900 1570 3986
Fragments arranged by size:
3986 1900 1570 636 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2513 4261 4825 6115 6876 7904
Size: 275 186 512 1748 564 1290 761 1028
Cuts at: 7904 1540
Size: 2301
Fragments arranged by size:
2301 1748 1290 1028 761 564 512 275
186
BsiHKAI G_wGCw'C
Cuts at: 2271 2469 5156 5642 5673 6726 6811 7882 2271
Size: 198 2687 486 31 1053 85 1071 3054
Fragments arranged by size:
3054 2687 1071 1053 486 198 85 31
BsmI GAATG_Cn'
Cuts at: 5917 6176 6269 5917
Size: 259 93 8313
Fragments arranged by size:
8313 259 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4976 4976
Size: 8665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2136 2205 2271 2469 2594 2991
Size: 399 241 105 69 66 198 125 397
Cuts at: 2991 3002 3128 3167 3513 3854 4040 4083 4167
Size: 11 126 39 346 341 186 43 84
Cuts at: 4167 4430 4528 4631 4666 4868 4931 4996 5156
Size: 263 98 103 35 202 63 65 160
Cuts at: 5156 5375 5390 5642 5673 5901 5984 6726 6811
Size: 219 15 252 31 228 83 742 85
Cuts at: 6811 7882 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 397 346 341 263
252 241 228 219 202 198 186 160
126 125 105 103 98 85 84 83
69 66 65 63 43 39 35 31
15 11
BspEI T'CCGG_A
Cuts at: 4900 8328 4900
Size: 3428 5237
BspHI T'CATG_A
Cuts at: 3672 6554 8284 3672
Size: 2882 1730 4053
Fragments arranged by size:
4053 2882 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3936 5395 6020 844
Size: 3092 1459 625 3489
Fragments arranged by size:
3489 3092 1459 625
BsrBI CCG'CTC
Cuts at: 1394 1823 6552 1394
Size: 429 4729 3507
Fragments arranged by size:
4729 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7160 7334 65
Size: 1183 5912 174 1396
Fragments arranged by size:
5912 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2563 3240 4188 4256 4260 4616 4804
Size: 110 753 677 948 68 4 356 188
Cuts at: 4804 5240 7309 7496 1700
Size: 436 2069 187 2869
Fragments arranged by size:
2869 2069 948 753 677 436 356 188
187 110 68 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8665
BssHII G'CGCG_C
Cuts at: 1925 2163 2219 4413 1925
Size: 238 56 2194 6177
Fragments arranged by size:
6177 2194 238 56
BssSI C'ACGA_G
Cuts at: 2380 5155 6725 7737 2380
Size: 2775 1570 1012 3308
Fragments arranged by size:
3308 2775 1570 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 3712 5610 794
Size: 1472 1446 1898 3849
Fragments arranged by size:
3849 1898 1472 1446
BstBI TT'CG_AA
Cuts at: 7469 7469
Size: 8665
BstEII G'GTnAC_C
Cuts at: 1184 5236 1184
Size: 4052 4613
BstXI CCAn_nnnn'nTGG
Cuts at: 2801 4408 5650 2801
Size: 1607 1242 5816
Fragments arranged by size:
5816 1607 1242
BstYI r'GATC_y
Cuts at: 1972 2149 5451 5466 5481 5995 6362 6747 6764
Size: 177 3302 15 15 514 367 385 17
Cuts at: 6764 8216 8302 8314 8660 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3302 1977 1452 514 385 367 346 177
86 17 15 15 12
Bsu36I CC'TnA_GG
Cuts at: 5572 5572
Size: 8665
BtgI C'CryG_G
Cuts at: 513 1065 2299 2614 2660 5263 513
Size: 552 1234 315 46 2603 3915
Fragments arranged by size:
3915 2603 1234 552 315 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7926
Fragments arranged by size:
7926 683 56
BtsI GCAGTG_nn'
Cuts at: 4617 5846 5854 6181 6975 6995 4617
Size: 1229 8 327 794 20 6287
Fragments arranged by size:
6287 1229 794 327 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2341 2376 529
Size: 35 1777 35 6818
Fragments arranged by size:
6818 1777 35 35
DraI TTT'AAA
Cuts at: 5969 5969
Size: 8665
DraIII CAC_nnn'GTG
Cuts at: 2560 2560
Size: 8665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7672 809
Size: 507 6356 1802
Fragments arranged by size:
6356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2224 2284
Size: 54 1425 50 275 18 223 171 60
Cuts at: 2284 2302 2826 3363 4258 4398 4465 4614 4822
Size: 18 524 537 895 140 67 149 208
Cuts at: 4822 6112 7001 8630 8
Size: 1290 889 1629 43
Fragments arranged by size:
1629 1425 1290 895 889 537 524 275
223 208 171 149 140 67 60 54
50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4258 4822 6112 1537
Size: 275 2446 564 1290 4090
Fragments arranged by size:
4090 2446 1290 564 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6595 1353
Size: 5242 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2489 3460 3903 3996 7252 7626
Size: 1064 138 900 971 443 93 3256 374
Cuts at: 7626 7772 387
Size: 146 1280
Fragments arranged by size:
3256 1280 1064 971 900 443 374 146
138 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2533 2614 2675 2986 3005 3266 4716
Size: 393 901 81 61 311 19 261 1450
Cuts at: 4716 5420 5456 5483 5555 6728 7325 8112 8380
Size: 704 36 27 72 1173 597 787 268
Cuts at: 8380 1239
Size: 1524
Fragments arranged by size:
1524 1450 1173 901 787 704 597 393
311 268 261 81 72 61 36 27
19
EcoNI CCTnn'n_nnAGG
Cuts at: 5402 5402
Size: 8665
EcoO109I rG'GnC_Cy
Cuts at: 2110 2132 2133 2376 2486 3034 3194 4036 4037
Size: 22 1 243 110 548 160 842 1
Cuts at: 4037 4524 4525 4662 4663 4670 5082 5371 5568
Size: 487 1 137 1 7 412 289 197
Cuts at: 5568 5704 2110
Size: 136 5071
Fragments arranged by size:
5071 842 548 487 412 289 243 197
160 137 136 110 22 7 1 1
1 1
EcoRI G'AATT_C
Cuts at: 5974 6352 5974
Size: 378 8287
FspI TGC'GCA
Cuts at: 7171 7171
Size: 8665
HaeII r_GCGC'y
Cuts at: 1678 2723 2811 2823 3317 3447 3658 3840 3872
Size: 1045 88 12 494 130 211 182 32
Cuts at: 3872 3998 4176 4189 5018 5277 5300 5436 5634
Size: 126 178 13 829 259 23 136 198
Cuts at: 5634 5679 5744 7812 1678
Size: 45 65 2068 2531
Fragments arranged by size:
2531 2068 1045 829 494 259 211 198
182 178 136 130 126 88 65 45
32 23 13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3548 3580 4874 4906 5165
Size: 32 135 32 1976 32 1294 32 259
Cuts at: 5165 5168 5197 5200 5212 5244 5639 5671 7306
Size: 3 29 3 12 32 395 32 1635
Cuts at: 7306 7338 7380 7412 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1976 1635 1294 395 259 135 42
32 32 32 32 32 32 32 32
29 12 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2038 2689 6009 6255 669
Size: 988 281 100 651 3320 246 3079
Fragments arranged by size:
3320 3079 988 651 281 246 100
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8665
HpaI GTT'AAC
Cuts at: 6255 6255
Size: 8665
KasI G'GCGC_C
Cuts at: 2719 2819 3313 3836 3868 3994 5273 5296 5432
Size: 100 494 523 32 126 1279 23 136
Cuts at: 5432 5740 2719
Size: 308 5644
Fragments arranged by size:
5644 1279 523 494 308 136 126 100
32 23
KpnI G_GTAC'C
Cuts at: 5990 5990
Size: 8665
MfeI C'AATT_G
Cuts at: 6264 6264
Size: 8665
MluI A'CGCG_T
Cuts at: 8541 8541
Size: 8665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3136 5085 5955 7779 7963 1305
Size: 1831 1949 870 1824 184 2007
Fragments arranged by size:
2007 1949 1831 1824 870 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2055 3365 10
Size: 54 1425 343 223 1310 5310
Fragments arranged by size:
5310 1425 1310 343 223 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 3359 6623 6982 7141 518
Size: 648 1029 1164 3264 359 159 2042
Fragments arranged by size:
3264 2042 1164 1029 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2581 2634 2770 2786 3174 3325 3411 3709
Size: 975 53 136 16 388 151 86 298
Cuts at: 3709 3965 4138 4281 4701 4908 5430 5607 5696
Size: 256 173 143 420 207 522 177 89
Cuts at: 5696 6081 6758 7906 8151 1606
Size: 385 677 1148 245 2120
Fragments arranged by size:
2120 1148 975 677 522 420 388 385
298 256 245 207 177 173 151 143
136 89 86 53 16
NaeI GCC'GGC
Cuts at: 2565 4190 4258 4262 4618 2565
Size: 1625 68 4 356 6612
Fragments arranged by size:
6612 1625 356 68 4
NarI GG'CG_CC
Cuts at: 2720 2820 3314 3837 3869 3995 5274 5297 5433
Size: 100 494 523 32 126 1279 23 136
Cuts at: 5433 5741 2720
Size: 308 5644
Fragments arranged by size:
5644 1279 523 494 308 136 126 100
32 23
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8113
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8665
NgoMIV G'CCGG_C
Cuts at: 2563 4188 4256 4260 4616 2563
Size: 1625 68 4 356 6612
Fragments arranged by size:
6612 1625 356 68 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8665
NotI GC'GGCC_GC
Cuts at: 6112 6112
Size: 8665
NspI r_CATG'y
Cuts at: 1132 1229 2743 3262 3292 3436 5692 6023 7568
Size: 97 1514 519 30 144 2256 331 1545
Cuts at: 7568 1132
Size: 2229
Fragments arranged by size:
2256 2229 1545 1514 519 331 144 97
30
PasI CC'CwG_GG
Cuts at: 1348 4738 4931 5745 1348
Size: 3390 193 814 4268
Fragments arranged by size:
4268 3390 814 193
PciI A'CATG_T
Cuts at: 2739 7564 2739
Size: 4825 3840
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2653 3244 5461 5527 1108
Size: 1545 591 2217 66 4246
Fragments arranged by size:
4246 2217 1545 591 66
PfoI T'CCnGG_A
Cuts at: 2523 4685 2523
Size: 2162 6503
PmeI GTTT'AAAC
Cuts at: 5969 5969
Size: 8665
PmlI CAC'GTG
Cuts at: 4078 4861 4078
Size: 783 7882
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6734 6766 8247 8279 6734
Size: 32 1481 32 7120
Fragments arranged by size:
7120 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4670 5082 4670
Size: 412 8253
PshAI GACnn'nnGTC
Cuts at: 1613 4854 1613
Size: 3241 5424
PsiI TTA'TAA
Cuts at: 6235 6235
Size: 8665
PspOMI G'GGCC_C
Cuts at: 2132 4036 4524 4662 5371 2132
Size: 1904 488 138 709 5426
Fragments arranged by size:
5426 1904 709 488 138
PspXI vC'TCGA_Gb
Cuts at: 5886 5886
Size: 8665
PstI C_TGCA'G
Cuts at: 830 3186 3333 3543 4047 4107 4143 6017 830
Size: 2356 147 210 504 60 36 1874 3478
Fragments arranged by size:
3478 2356 1874 504 210 147 60 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8665
PvuII CAG'CTG
Cuts at: 2770 4138 5607 2770
Size: 1368 1469 5828
Fragments arranged by size:
5828 1469 1368
RsrII CG'GwC_CG
Cuts at: 5288 5288
Size: 8665
SalI G'TCGA_C
Cuts at: 1936 2687 6007 1936
Size: 751 3320 4594
Fragments arranged by size:
4594 3320 751
SanDI GG'GwC_CC
Cuts at: 5082 5082
Size: 8665
SbfI CC_TGCA'GG
Cuts at: 3186 6017 3186
Size: 2831 5834
ScaI AGT'ACT
Cuts at: 4889 6913 8625 4889
Size: 2024 1712 4929
Fragments arranged by size:
4929 2024 1712
SfcI C'TryA_G
Cuts at: 826 1045 3182 3329 3539 4043 4103 4139 5489
Size: 219 2137 147 210 504 60 36 1350
Cuts at: 5489 6013 7148 7829 8020 826
Size: 524 1135 681 191 1471
Fragments arranged by size:
2137 1471 1350 1135 681 524 504 219
210 191 147 60 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 3765 3765
Size: 8665
SfoI GGC'GCC
Cuts at: 2721 2821 3315 3838 3870 3996 5275 5298 5434
Size: 100 494 523 32 126 1279 23 136
Cuts at: 5434 5742 2721
Size: 308 5644
Fragments arranged by size:
5644 1279 523 494 308 136 126 100
32 23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8665
SmaI CCC'GGG
Cuts at: 1872 2310 2374 2712 2817 3100 3748 4150 4626
Size: 438 64 338 105 283 648 402 476
Cuts at: 4626 5376 5992 1872
Size: 750 616 4545
Fragments arranged by size:
4545 750 648 616 476 438 402 338
283 105 64
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5886 6769 7670 7932 820
Size: 197 926 3943 883 901 262 1553
Fragments arranged by size:
3943 1553 926 901 883 262 197
SnaBI TAC'GTA
Cuts at: 493 6456 493
Size: 5963 2702
SpeI A'CTAG_T
Cuts at: 152 5209 152
Size: 5057 3608
SphI G_CATG'C
Cuts at: 1132 3262 3292 3436 6023 1132
Size: 2130 30 144 2587 3774
Fragments arranged by size:
3774 2587 2130 144 30
SrfI GCCC'GGGC
Cuts at: 5376 5376
Size: 8665
SspI AAT'ATT
Cuts at: 5 52 6589 5
Size: 47 6537 2081
Fragments arranged by size:
6537 2081 47
StuI AGG'CCT
Cuts at: 1517 2430 3483 4128 5810 1517
Size: 913 1053 645 1682 4372
Fragments arranged by size:
4372 1682 1053 913 645
StyI C'CwwG_G
Cuts at: 513 1065 2677 4449 5880 6104 513
Size: 552 1612 1772 1431 224 3074
Fragments arranged by size:
3074 1772 1612 1431 552 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 4613 5117 5305 5861 6692 6709
Size: 1255 534 2499 504 188 556 831 17
Cuts at: 6709 6862 7047 7492 325
Size: 153 185 445 1498
Fragments arranged by size:
2499 1498 1255 831 556 534 504 445
188 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4887 6911 8623
Size: 274 80 33 51 1443 2910 2024 1712
Cuts at: 8623 96
Size: 138
Fragments arranged by size:
2910 2024 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 8625 8657 3254
Size: 32 5339 32 3262
Fragments arranged by size:
5339 3262 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4833 1316
Size: 3517 5148
XbaI T'CTAG_A
Cuts at: 6001 6001
Size: 8665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3241 4771 2011
Size: 1230 1530 5905
Fragments arranged by size:
5905 1530 1230
XhoI C'TCGA_G
Cuts at: 1943 5886 1943
Size: 3943 4722
XmaI C'CCGG_G
Cuts at: 1870 2308 2372 2710 2815 3098 3746 4148 4624
Size: 438 64 338 105 283 648 402 476
Cuts at: 4624 5374 5990 1870
Size: 750 616 4545
Fragments arranged by size:
4545 750 648 616 476 438 402 338
283 105 64
XmnI GAAnn'nnTTC
Cuts at: 6794 6794
Size: 8665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6473 276
Size: 53 83 186 936 4939 2468
Fragments arranged by size:
4939 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PsrI
SacI SacII SapI SexAI SwaI