Restriction map for
pFN21AE0071
(Circular) (Six-base) MAPSORT of: pFN21AE0071.seq Check: 449 from: 1 to: 6874
pFN21AE0071 6874 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2813 2921 2984 3383 3416 3444 3497 2813
Size: 108 63 399 33 28 53 6190
Fragments arranged by size:
6190 399 108 63 53 33 28
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4684 278
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2271 4217 1937
Size: 334 1946 4594
Fragments arranged by size:
4594 1946 334
Acc65I G'GTAC_C
Cuts at: 4195 4195
Size: 6874
AclI AA'CG_TT
Cuts at: 1563 5001 5374 1563
Size: 3438 373 3063
Fragments arranged by size:
3438 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2263 4937 6321 6589 1632
Size: 473 158 2674 1384 268 1917
Fragments arranged by size:
2674 1917 1384 473 268 158
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6677
AflIII A'CryG_T
Cuts at: 1203 5773 6750 1203
Size: 4570 977 1327
Fragments arranged by size:
4570 1327 977
AgeI A'CCGG_T
Cuts at: 5705 5705
Size: 6874
AhdI GACnn_n'nnGTC
Cuts at: 5603 5603
Size: 6874
AleI CACnn'nnGTG
Cuts at: 1166 2161 2258 1166
Size: 995 97 5782
Fragments arranged by size:
5782 995 97
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6874
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3038 3070 3383 3415 3038
Size: 32 313 32 6497
Fragments arranged by size:
6497 313 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2491 2869 3435 3559 4617 6189 2491
Size: 378 566 124 1058 1572 3176
Fragments arranged by size:
3176 1572 1058 566 378 124
ApaI G_GGCC'C
Cuts at: 2661 3584 2661
Size: 923 5951
ApaLI G'TGCA_C
Cuts at: 4931 6087 4931
Size: 1156 5718
ApoI r'AATT_y
Cuts at: 1493 4183 4409 4561 6670 1493
Size: 2690 226 152 2109 1697
Fragments arranged by size:
2690 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5428 160
Size: 872 4396 1606
Fragments arranged by size:
4396 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
AvaI C'yCGr_G
Cuts at: 1870 1943 2222 2586 3041 3310 3528 3566 3577
Size: 73 279 364 455 269 218 38 11
Cuts at: 3577 4084 4199 1870
Size: 507 115 4545
Fragments arranged by size:
4545 507 455 364 279 269 218 115
73 38 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2758 2791 2953 2986 3043 3076 2758
Size: 33 162 33 57 33 6556
Fragments arranged by size:
6556 162 57 33 33 33
BamHI G'GATC_C
Cuts at: 4204 4571 4204
Size: 367 6507
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2357 2457 2801 2969
Size: 325 217 627 513 57 100 344 168
Cuts at: 2969 3254 3371 4195 5650 618
Size: 285 117 824 1455 1842
Fragments arranged by size:
1842 1455 824 627 513 344 325 285
217 168 117 100 57
BanII G_rGCy'C
Cuts at: 1391 2069 2661 3584 4013 4193 1391
Size: 678 592 923 429 180 4072
Fragments arranged by size:
4072 923 678 592 429 180
BbeI G_GCGC'C
Cuts at: 2304 2304
Size: 6874
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5903
BbvCI CC'TCA_GC
Cuts at: 3716 3716
Size: 6874
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2665 2699 3022 3056 5065 5099 1903
Size: 34 728 34 323 34 2009 34 3678
Fragments arranged by size:
3678 2009 728 323 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2786 4766 5976 2786
Size: 1980 1210 3684
Fragments arranged by size:
3684 1980 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6874
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2145 2838 3072
Size: 107 122 71 631 605 473 693 234
Cuts at: 3072 3189 3303 3384 3417 5485 136
Size: 117 114 81 33 2068 1525
Fragments arranged by size:
2068 1525 693 631 605 473 234 122
117 114 107 81 71 33
BglII A'GATC_T
Cuts at: 6869 6869
Size: 6874
BlpI GC'TnA_GC
Cuts at: 4291 4291
Size: 6874
Bme1580I G_kGCm'C
Cuts at: 1790 2360 2661 3374 3445 3529 3584 4935 6091
Size: 570 301 713 71 84 55 1351 1156
Cuts at: 6091 1790
Size: 2573
Fragments arranged by size:
2573 1351 1156 713 570 301 84 71
55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6874
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4605 5563 442
Size: 416 53 83 399 24 3188 958 1753
Fragments arranged by size:
3188 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6874
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 6874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2083 5534 1239
Size: 844 3451 2579
Fragments arranged by size:
3451 2579 844
Bpu10I CC'TnA_GC
Cuts at: 3716 3716
Size: 6874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2501 3929 4999 5864 6162 2180
Size: 321 1428 1070 865 298 2892
Fragments arranged by size:
2892 1428 1070 865 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3761 5537 882
Size: 617 2262 1776 2219
Fragments arranged by size:
2262 2219 1776 617
BsaAI yAC'GTr
Cuts at: 493 1204 4665 493
Size: 711 3461 2702
Fragments arranged by size:
3461 2702 711
BsaBI GATnn'nnATC
Cuts at: 6868 6868
Size: 6874
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2268 2301 4681 5063
Size: 53 83 186 936 735 33 2380 382
Cuts at: 5063 5698 275
Size: 635 1451
Fragments arranged by size:
2380 1451 936 735 635 382 186 83
53 33
BsaWI w'CCGG_w
Cuts at: 5307 5705 5979 6126 6537 5307
Size: 398 274 147 411 5644
Fragments arranged by size:
5644 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2380 2410 2621 2651 3038
Size: 30 165 30 977 30 211 30 387
Cuts at: 3038 3068 3307 3332 3337 3362 3383 3413 1178
Size: 30 239 25 5 25 21 30 4639
Fragments arranged by size:
4639 977 387 239 211 165 30 30
30 30 30 30 25 25 21 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2489 3340 4003 4037 4154 1186
Size: 1303 851 663 34 117 3906
Fragments arranged by size:
3906 1303 851 663 117 34
BseYI C'CCAG_C
Cuts at: 2119 2263 2841 2983 3005 3583 3682 3739 3913
Size: 144 578 142 22 578 99 57 174
Cuts at: 3913 6077 6573 2119
Size: 2164 496 2420
Fragments arranged by size:
2420 2164 578 578 496 174 144 142
99 57 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 2477 2650 2758 2863 2899 3016
Size: 573 482 259 173 108 105 36 117
Cuts at: 3016 3388 3956 1163
Size: 372 568 4081
Fragments arranged by size:
4081 573 568 482 372 259 173 117
108 105 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2506 4324 5085 6113 1540
Size: 275 186 505 1818 761 1028 2301
Fragments arranged by size:
2301 1818 1028 761 505 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 2069 3149 3465 4935 5020 6091 2040
Size: 29 1080 316 1470 85 1071 2823
Fragments arranged by size:
2823 1470 1080 1071 316 85 29
BsmI GAATG_Cn'
Cuts at: 2735 4385 4478 2735
Size: 1650 93 5131
Fragments arranged by size:
5131 1650 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3755 3755
Size: 6874
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2069 2360 2661 3149 3374 3445
Size: 399 250 29 291 301 488 225 71
Cuts at: 3445 3465 3529 3584 4013 4193 4935 5020 6091
Size: 20 64 55 429 180 742 85 1071
Cuts at: 6091 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 488 429 399 301 291
250 225 180 85 71 64 55 29
20
BspEI T'CCGG_A
Cuts at: 6537 6537
Size: 6874
BspHI T'CATG_A
Cuts at: 4763 6493 4763
Size: 1730 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2499 2813 2921 2984 3071 3383 3416 3444
Size: 1655 314 108 63 87 312 33 28
Cuts at: 3444 3497 4229 844
Size: 53 732 3489
Fragments arranged by size:
3489 1655 732 314 312 108 87 63
53 33 28
BsrBI CCG'CTC
Cuts at: 1394 1823 4761 1394
Size: 429 2938 3507
Fragments arranged by size:
3507 2938 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5369 5543 65
Size: 1183 4121 174 1396
Fragments arranged by size:
4121 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2757 2952 3302 3732 5518 5705 1700
Size: 110 947 195 350 430 1786 187 2869
Fragments arranged by size:
2869 1786 947 430 350 195 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6874
BssHII G'CGCG_C
Cuts at: 1925 2019 1925
Size: 94 6780
BssSI C'ACGA_G
Cuts at: 3494 3932 4934 5946 3494
Size: 438 1002 1012 4422
Fragments arranged by size:
4422 1012 1002 438
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2016 2136 3435 794
Size: 1222 120 1299 4233
Fragments arranged by size:
4233 1299 1222 120
BstBI TT'CG_AA
Cuts at: 2079 5678 2079
Size: 3599 3275
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6874
BstXI CCAn_nnnn'nTGG
Cuts at: 3265 3636 3265
Size: 371 6503
BstYI r'GATC_y
Cuts at: 1972 3037 4204 4571 4956 4973 6425 6511 6523
Size: 1065 1167 367 385 17 1452 86 12
Cuts at: 6523 6869 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1167 1065 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4015 4015
Size: 6874
BtgI C'CryG_G
Cuts at: 513 1065 2004 2140 2797 3186 3868 513
Size: 552 939 136 657 389 682 3519
Fragments arranged by size:
3519 939 682 657 552 389 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6135
Fragments arranged by size:
6135 683 56
BtsI GCAGTG_nn'
Cuts at: 2828 3445 4390 5184 5204 2828
Size: 617 945 794 20 4498
Fragments arranged by size:
4498 945 794 617 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6874
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4152 4187 529
Size: 35 3588 35 3216
Fragments arranged by size:
3588 3216 35 35
DraI TTT'AAA
Cuts at: 4178 4178
Size: 6874
DraIII CAC_nnn'GTG
Cuts at: 3996 3996
Size: 6874
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3812 3962 5881 809
Size: 507 2496 150 1919 1802
Fragments arranged by size:
2496 1919 1802 507 150
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2351 2445 2503
Size: 54 1425 50 275 18 521 94 58
Cuts at: 2503 2602 3642 3783 4321 5210 6839 8
Size: 99 1040 141 538 889 1629 43
Fragments arranged by size:
1629 1425 1040 889 538 521 275 141
99 94 58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2503 4321 1537
Size: 275 691 1818 4090
Fragments arranged by size:
4090 1818 691 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3241 4804 1353
Size: 1888 1563 3423
Fragments arranged by size:
3423 1888 1563
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3875 5461 5835 5981 387
Size: 1064 138 2286 1586 374 146 1280
Fragments arranged by size:
2286 1586 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2067 2067
Size: 6874
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 2105 2263 4937 5534 6321 6589
Size: 393 451 22 158 2674 597 787 268
Cuts at: 6589 1239
Size: 1524
Fragments arranged by size:
2674 1524 787 597 451 393 268 158
22
EcoNI CCTnn'n_nnAGG
Cuts at: 3935 3935
Size: 6874
EcoO109I rG'GnC_Cy
Cuts at: 2488 2657 2658 2848 2866 3542 3940 4032 2488
Size: 169 1 190 18 676 398 92 5330
Fragments arranged by size:
5330 676 398 190 169 92 18 1
EcoRI G'AATT_C
Cuts at: 4183 4561 4183
Size: 378 6496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3880 3913 3880
Size: 33 6841
FspI TGC'GCA
Cuts at: 5380 5380
Size: 6874
HaeII r_GCGC'y
Cuts at: 1678 2250 2304 3885 6021 1678
Size: 572 54 1581 2136 2531
Fragments arranged by size:
2531 2136 1581 572 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2465 2497 3332 3364 5515
Size: 32 135 32 893 32 835 32 2151
Cuts at: 5515 5547 5589 5621 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2151 893 835 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2104 2272 4218 4464 669
Size: 988 281 166 168 1946 246 3079
Fragments arranged by size:
3079 1946 988 281 246 168 166
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6874
HpaI GTT'AAC
Cuts at: 4464 4464
Size: 6874
KasI G'GCGC_C
Cuts at: 2300 2300
Size: 6874
KpnI G_GTAC'C
Cuts at: 4199 4199
Size: 6874
MfeI C'AATT_G
Cuts at: 4473 4473
Size: 6874
MluI A'CGCG_T
Cuts at: 6750 6750
Size: 6874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5988 6172 1305
Size: 4683 184 2007
Fragments arranged by size:
4683 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2447 3785 10
Size: 54 1425 343 615 1338 3099
Fragments arranged by size:
3099 1425 1338 615 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2161 2258 3510 4832 5191 5350 518
Size: 648 995 97 1252 1322 359 159 2042
Fragments arranged by size:
2042 1322 1252 995 648 359 159 97
MspA1I CmG'CkG
Cuts at: 1606 2142 2502 3174 3180 3294 3587 3695 3917
Size: 536 360 672 6 114 293 108 222
Cuts at: 3917 4290 4967 6115 6360 1606
Size: 373 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 536 373 360 293
245 222 114 108 6
NaeI GCC'GGC
Cuts at: 2954 3304 2954
Size: 350 6524
NarI GG'CG_CC
Cuts at: 2301 2301
Size: 6874
NcoI C'CATG_G
Cuts at: 513 1065 2004 2797 513
Size: 552 939 793 4590
Fragments arranged by size:
4590 939 793 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6874
NgoMIV G'CCGG_C
Cuts at: 2952 3302 2952
Size: 350 6524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6874
NotI GC'GGCC_GC
Cuts at: 2503 4321 2503
Size: 1818 5056
NspI r_CATG'y
Cuts at: 1132 1229 4232 5777 1132
Size: 97 3003 1545 2229
Fragments arranged by size:
3003 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3636 4006 1348
Size: 2288 370 4216
Fragments arranged by size:
4216 2288 370
PciI A'CATG_T
Cuts at: 5773 5773
Size: 6874
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6874
PmeI GTTT'AAAC
Cuts at: 4178 4178
Size: 6874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3070 3383 3415 4943 4975 6456 6488 3038
Size: 32 313 32 1528 32 1481 32 3424
Fragments arranged by size:
3424 1528 1481 313 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2866 3542 3940 4032 2488
Size: 378 676 398 92 5330
Fragments arranged by size:
5330 676 398 378 92
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6874
PsiI TTA'TAA
Cuts at: 4444 4444
Size: 6874
PspOMI G'GGCC_C
Cuts at: 2657 3580 2657
Size: 923 5951
PstI C_TGCA'G
Cuts at: 830 2094 3233 3440 3726 4226 830
Size: 1264 1139 207 286 500 3478
Fragments arranged by size:
3478 1264 1139 500 286 207
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6874
PvuII CAG'CTG
Cuts at: 3174 3174
Size: 6874
SacI G_AGCT'C
Cuts at: 2069 2069
Size: 6874
SacII CC_GC'GG
Cuts at: 2143 2143
Size: 6874
SalI G'TCGA_C
Cuts at: 1936 2270 4216 1936
Size: 334 1946 4594
Fragments arranged by size:
4594 1946 334
SanDI GG'GwC_CC
Cuts at: 3542 4032 3542
Size: 490 6384
SapI GCTCTTCn'nnn_
Cuts at: 3241 3241
Size: 6874
SbfI CC_TGCA'GG
Cuts at: 4226 4226
Size: 6874
ScaI AGT'ACT
Cuts at: 2522 5122 6834 2522
Size: 2600 1712 2562
Fragments arranged by size:
2600 2562 1712
SfcI C'TryA_G
Cuts at: 826 1045 2090 3229 3436 3722 4222 5357 6038
Size: 219 1045 1139 207 286 500 1135 681
Cuts at: 6038 6229 826
Size: 191 1471
Fragments arranged by size:
1471 1139 1135 1045 681 500 286 219
207 191
SfoI GGC'GCC
Cuts at: 2302 2302
Size: 6874
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6874
SmaI CCC'GGG
Cuts at: 1872 2588 3579 4201 1872
Size: 716 991 622 4545
Fragments arranged by size:
4545 991 716 622
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2195 2516 3041 3944 4978 5879
Size: 197 926 252 321 525 903 1034 901
Cuts at: 5879 6141 820
Size: 262 1553
Fragments arranged by size:
1553 1034 926 903 901 525 321 262
252 197
SnaBI TAC'GTA
Cuts at: 493 4665 493
Size: 4172 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6874
SphI G_CATG'C
Cuts at: 1132 4232 1132
Size: 3100 3774
SspI AAT'ATT
Cuts at: 5 52 4798 5
Size: 47 4746 2081
Fragments arranged by size:
4746 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6874
StyI C'CwwG_G
Cuts at: 513 1065 2004 2797 4313 513
Size: 552 939 793 1516 3074
Fragments arranged by size:
3074 1516 939 793 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2465 3628 4901 4918 5071 5256 5701
Size: 1255 885 1163 1273 17 153 185 445
Cuts at: 5701 325
Size: 1498
Fragments arranged by size:
1498 1273 1255 1163 885 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 5120 6832
Size: 274 80 33 51 1443 543 2600 1712
Cuts at: 6832 96
Size: 138
Fragments arranged by size:
2600 1712 1443 543 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2380 2412 2621 2653 2720 2752 3307 3339 3424
Size: 32 209 32 67 32 555 32 85
Cuts at: 3424 3456 6834 6866 2380
Size: 32 3378 32 2388
Fragments arranged by size:
3378 2388 555 209 85 67 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6874
XbaI T'CTAG_A
Cuts at: 4210 4210
Size: 6874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2612 2980 3778 2612
Size: 368 798 5708
Fragments arranged by size:
5708 798 368
XhoI C'TCGA_G
Cuts at: 1943 3041 1943
Size: 1098 5776
XmaI C'CCGG_G
Cuts at: 1870 2586 3577 4199 1870
Size: 716 991 622 4545
Fragments arranged by size:
4545 991 716 622
XmnI GAAnn'nnTTC
Cuts at: 3395 3896 5003 3395
Size: 501 1107 5266
Fragments arranged by size:
5266 1107 501
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4682 276
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SexAI SfiI SrfI SwaI