Restriction map for
pFN21AE0068
(Circular) (Six-base) MAPSORT of: pFN21AE0068.seq Check: 3169 from: 1 to: 6241
pFN21AE0068 6241 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 1937
Size: 1647 4594
Acc65I G'GTAC_C
Cuts at: 3562 3562
Size: 6241
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2545 4304 5688 5956 1632
Size: 913 1759 1384 268 1917
Fragments arranged by size:
1917 1759 1384 913 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 5140 6117 1203
Size: 3937 977 1327
Fragments arranged by size:
3937 1327 977
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 4970 4970
Size: 6241
AleI CACnn'nnGTG
Cuts at: 1166 2621 2826 1166
Size: 1455 205 4581
Fragments arranged by size:
4581 1455 205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2844 1184
Size: 1660 4581
AlwNI CAG_nnn'CTG
Cuts at: 2644 2890 3984 5556 2644
Size: 246 1094 1572 3329
Fragments arranged by size:
3329 1572 1094 246
ApaI G_GGCC'C
Cuts at: 3195 3195
Size: 6241
ApaLI G'TGCA_C
Cuts at: 3275 4298 5454 3275
Size: 1023 1156 4062
Fragments arranged by size:
4062 1156 1023
ApoI r'AATT_y
Cuts at: 1493 3550 3776 3928 6037 1493
Size: 2057 226 152 2109 1697
Fragments arranged by size:
2109 2057 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 2415 2784 3131 3566 1870
Size: 73 472 369 347 435 4545
Fragments arranged by size:
4545 472 435 369 347 73
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2623 3048 3335 3562 5017
Size: 325 217 627 836 425 287 227 1455
Cuts at: 5017 618
Size: 1842
Fragments arranged by size:
1842 1455 836 627 425 325 287 227
217
BanII G_rGCy'C
Cuts at: 1391 2984 3195 3560 1391
Size: 1593 211 365 4072
Fragments arranged by size:
4072 1593 365 211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2149 2536 928
Size: 971 250 387 4633
Fragments arranged by size:
4633 971 387 250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2056 2090 4432 4466 1903
Size: 34 119 34 2342 34 3678
Fragments arranged by size:
3678 2342 119 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4133 5343 4133
Size: 1210 5031
BclI T'GATC_A
Cuts at: 1548 2331 1548
Size: 783 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4852 136
Size: 107 122 71 631 605 3180 1525
Fragments arranged by size:
3180 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2556 3225 6236 2556
Size: 669 3011 2561
Fragments arranged by size:
3011 2561 669
BlpI GC'TnA_GC
Cuts at: 2104 3658 2104
Size: 1554 4687
Bme1580I G_kGCm'C
Cuts at: 1790 2628 2833 3195 3279 4302 5458 1790
Size: 838 205 362 84 1023 1156 2573
Fragments arranged by size:
2573 1156 1023 838 362 205 84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3189 3972 4930
Size: 416 53 83 399 24 1772 783 958
Cuts at: 4930 442
Size: 1753
Fragments arranged by size:
1772 1753 958 783 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2552 2783 4901 1239
Size: 806 507 231 2118 2579
Fragments arranged by size:
2579 2118 806 507 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4366 5231 5529 4366
Size: 865 298 5078
Fragments arranged by size:
5078 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3401 4904 882
Size: 617 1902 1503 2219
Fragments arranged by size:
2219 1902 1503 617
BsaAI yAC'GTr
Cuts at: 493 1204 3394 4032 493
Size: 711 2190 638 2702
Fragments arranged by size:
2702 2190 711 638
BsaBI GATnn'nnATC
Cuts at: 3008 3386 6235 3008
Size: 378 2849 3014
Fragments arranged by size:
3014 2849 378
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2988 4048 4430 5065
Size: 53 83 186 936 1455 1060 382 635
Cuts at: 5065 275
Size: 1451
Fragments arranged by size:
1455 1451 1060 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3403 4674 5072 5346 5493 5904 3403
Size: 1271 398 274 147 411 3740
Fragments arranged by size:
3740 1271 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3502 3532 1178
Size: 30 165 30 2099 30 3887
Fragments arranged by size:
3887 2099 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2740 2846 3362 3491 1186
Size: 1554 106 516 129 3936
Fragments arranged by size:
3936 1554 516 129 106
BseYI C'CCAG_C
Cuts at: 2425 3037 3500 5444 5940 2425
Size: 612 463 1944 496 2726
Fragments arranged by size:
2726 1944 612 496 463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2457 2804 1163
Size: 573 721 347 4600
Fragments arranged by size:
4600 721 573 347
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3691 4452 5480 1540
Size: 275 186 1690 761 1028 2301
Fragments arranged by size:
2301 1690 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2874 3111 3279 4302 4387 5458 2874
Size: 237 168 1023 85 1071 3657
Fragments arranged by size:
3657 1071 1023 237 168 85
BsmI GAATG_Cn'
Cuts at: 3752 3845 3752
Size: 93 6148
BsmBI CGTCTCn'nnnn_
Cuts at: 2251 2251
Size: 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2628 2833 2874 2984 3111 3195 3279
Size: 399 838 205 41 110 127 84 84
Cuts at: 3279 3560 4302 4387 5458 1391
Size: 281 742 85 1071 2174
Fragments arranged by size:
2174 1071 838 742 399 281 205 127
110 85 84 84 41
BspEI T'CCGG_A
Cuts at: 3403 5904 3403
Size: 2501 3740
BspHI T'CATG_A
Cuts at: 4130 5860 4130
Size: 1730 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3596 844
Size: 2752 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2957 3034 4128 1394
Size: 429 1134 77 1094 3507
Fragments arranged by size:
3507 1134 1094 429 77
BsrDI GCAATG_nn'
Cuts at: 65 1248 2279 4736 4910 65
Size: 1183 1031 2457 174 1396
Fragments arranged by size:
2457 1396 1183 1031 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2599 2879 3062 4885 5072 1700
Size: 110 789 280 183 1823 187 2869
Fragments arranged by size:
2869 1823 789 280 187 183 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6241
BssHII G'CGCG_C
Cuts at: 1925 2794 1925
Size: 869 5372
BssSI C'ACGA_G
Cuts at: 2285 4301 5313 2285
Size: 2016 1012 3213
Fragments arranged by size:
3213 2016 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3055 794
Size: 2261 3980
BstBI TT'CG_AA
Cuts at: 5045 5045
Size: 6241
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6241
BstXI CCAn_nnnn'nTGG
Cuts at: 2368 2470 2368
Size: 102 6139
BstYI r'GATC_y
Cuts at: 1972 2556 3225 3571 3938 4323 4340 5792 5878
Size: 584 669 346 367 385 17 1452 86
Cuts at: 5878 5890 6236 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 669 584 385 367 346 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4750
Fragments arranged by size:
4750 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3157 487
Size: 683 56 1931 3571
Fragments arranged by size:
3571 1931 683 56
BtsI GCAGTG_nn'
Cuts at: 3279 3375 3757 4551 4571 3279
Size: 96 382 794 20 4949
Fragments arranged by size:
4949 794 382 96 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6206
DraI TTT'AAA
Cuts at: 3545 3545
Size: 6241
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5248 809
Size: 507 3932 1802
Fragments arranged by size:
3932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 2597 2877
Size: 54 1425 50 275 18 555 212 280
Cuts at: 2877 2881 3060 3688 4577 6206 8
Size: 4 179 628 889 1629 43
Fragments arranged by size:
1629 1425 889 628 555 280 275 212
179 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4171 1353
Size: 2818 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4828 5202 5348 387
Size: 1064 138 3239 374 146 1280
Fragments arranged by size:
3239 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2545 2552 2783 4304 4901 5688
Size: 393 413 500 7 231 1521 597 787
Cuts at: 5688 5956 1239
Size: 268 1524
Fragments arranged by size:
1524 1521 787 597 500 413 393 268
231 7
EcoO109I rG'GnC_Cy
Cuts at: 2322 3127 2322
Size: 805 5436
EcoRI G'AATT_C
Cuts at: 3550 3928 3550
Size: 378 5863
FseI GG_CCGG'CC
Cuts at: 2883 2883
Size: 6241
FspI TGC'GCA
Cuts at: 4747 4747
Size: 6241
HaeII r_GCGC'y
Cuts at: 1678 2457 2615 5388 1678
Size: 779 158 2773 2531
Fragments arranged by size:
2773 2531 779 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4882 4914 4956 4988 1373
Size: 32 135 32 3310 32 42 32 2626
Fragments arranged by size:
3310 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2140 2995 3585 3831 669
Size: 988 281 202 855 590 246 3079
Fragments arranged by size:
3079 988 855 590 281 246 202
HindIII A'AGCT_T
Cuts at: 748 3467 748
Size: 2719 3522
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KpnI G_GTAC'C
Cuts at: 3566 3566
Size: 6241
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2518 5355 5539 1305
Size: 1213 2837 184 2007
Fragments arranged by size:
2837 2007 1213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 10
Size: 54 1425 343 555 3864
Fragments arranged by size:
3864 1425 555 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2366 2621 2747 2826 2867 3002 3392
Size: 648 1200 255 126 79 41 135 390
Cuts at: 3392 4199 4558 4717 518
Size: 807 359 159 2042
Fragments arranged by size:
2042 1200 807 648 390 359 255 159
135 126 79 41
MspA1I CmG'CkG
Cuts at: 1606 2641 2890 3657 4334 5482 5727 1606
Size: 1035 249 767 677 1148 245 2120
Fragments arranged by size:
2120 1148 1035 767 677 249 245
NaeI GCC'GGC
Cuts at: 2881 3064 2881
Size: 183 6058
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4750
Fragments arranged by size:
4750 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6241
NgoMIV G'CCGG_C
Cuts at: 2879 3062 2879
Size: 183 6058
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 2773 3599 5144 1132
Size: 97 1544 826 1545 2229
Fragments arranged by size:
2229 1545 1544 826 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6241
PciI A'CATG_T
Cuts at: 5140 5140
Size: 6241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6241
PfoI T'CCnGG_A
Cuts at: 3321 3321
Size: 6241
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 5823 5855 4310
Size: 32 1481 32 4696
Fragments arranged by size:
4696 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2322 3127 2322
Size: 805 5436
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6241
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PspOMI G'GGCC_C
Cuts at: 3191 3191
Size: 6241
PstI C_TGCA'G
Cuts at: 830 2640 3237 3593 830
Size: 1810 597 356 3478
Fragments arranged by size:
3478 1810 597 356
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
PvuII CAG'CTG
Cuts at: 2641 2890 2641
Size: 249 5992
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SanDI GG'GwC_CC
Cuts at: 2322 3127 2322
Size: 805 5436
SbfI CC_TGCA'GG
Cuts at: 3593 3593
Size: 6241
ScaI AGT'ACT
Cuts at: 4489 6201 4489
Size: 1712 4529
SfcI C'TryA_G
Cuts at: 826 1045 2449 2636 3233 3340 3589 4724 5405
Size: 219 1404 187 597 107 249 1135 681
Cuts at: 5405 5596 826
Size: 191 1471
Fragments arranged by size:
1471 1404 1135 681 597 249 219 191
187 107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 2786 3568 1872
Size: 914 782 4545
Fragments arranged by size:
4545 914 782
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4345 5246 5508 820
Size: 197 926 2402 901 262 1553
Fragments arranged by size:
2402 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 3599 1132
Size: 2467 3774
SrfI GCCC'GGGC
Cuts at: 2786 2786
Size: 6241
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6241
StyI C'CwwG_G
Cuts at: 513 1065 2004 2388 3411 3525 3680 513
Size: 552 939 384 1023 114 155 3074
Fragments arranged by size:
3074 1023 939 552 384 155 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2050 2534 3236 4268 4285 4438 4623
Size: 1255 470 484 702 1032 17 153 185
Cuts at: 4623 5068 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1032 702 484 470 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4487 6199 96
Size: 274 80 33 51 1443 2510 1712 138
Fragments arranged by size:
2510 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3502 3534 6201 6233 3502
Size: 32 2667 32 3510
Fragments arranged by size:
3510 2667 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6241
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2257
Size: 6241
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6241
XmaI C'CCGG_G
Cuts at: 1870 2784 3566 1870
Size: 914 782 4545
Fragments arranged by size:
4545 914 782
XmnI GAAnn'nnTTC
Cuts at: 4370 4370
Size: 6241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SwaI