Restriction map for
pFN21AE0067
(Circular) (Six-base) MAPSORT of: pFN21AE0067.seq Check: 7173 from: 1 to: 9046
pFN21AE0067 9046 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3367 3367
Size: 9046
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4631 6856 278
Size: 53 83 186 936 3095 2225 2468
Fragments arranged by size:
3095 2468 2225 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 4136 6389 1937
Size: 990 1209 2253 4594
Fragments arranged by size:
4594 2253 1209 990
Acc65I G'GTAC_C
Cuts at: 2032 4044 6367 2032
Size: 2012 2323 4711
Fragments arranged by size:
4711 2323 2012
AclI AA'CG_TT
Cuts at: 1563 7173 7546 1563
Size: 5610 373 3063
Fragments arranged by size:
5610 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3443 3500 5104 5364 6234 7109 8493 8761
Size: 1811 57 1604 260 870 875 1384 268
Cuts at: 8761 1632
Size: 1917
Fragments arranged by size:
1917 1811 1604 1384 875 870 268 260
57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8849
AflIII A'CryG_T
Cuts at: 1203 7945 8922 1203
Size: 6742 977 1327
Fragments arranged by size:
6742 1327 977
AgeI A'CCGG_T
Cuts at: 2093 7877 2093
Size: 5784 3262
AhdI GACnn_n'nnGTC
Cuts at: 7775 7775
Size: 9046
AleI CACnn'nnGTG
Cuts at: 1166 3704 1166
Size: 2538 6508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6339 1184
Size: 5155 3891
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4404 4436 6069 6101 4404
Size: 32 1633 32 7349
Fragments arranged by size:
7349 1633 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2447 3068 6212 6789 8361 2447
Size: 621 3144 577 1572 3132
Fragments arranged by size:
3144 3132 1572 621 577
ApaI G_GGCC'C
Cuts at: 6120 6120
Size: 9046
ApaLI G'TGCA_C
Cuts at: 3240 7103 8259 3240
Size: 3863 1156 4027
Fragments arranged by size:
4027 3863 1156
ApoI r'AATT_y
Cuts at: 1493 2628 2691 3773 3981 4754 5067 5080 6355
Size: 1135 63 1082 208 773 313 13 1275
Cuts at: 6355 6581 6733 8842 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1275 1135 1082 773 313 226
208 152 63 13
AseI AT'TA_AT
Cuts at: 160 1032 7600 160
Size: 872 6568 1606
Fragments arranged by size:
6568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9046
AvaI C'yCGr_G
Cuts at: 1870 1943 2565 5694 6371 1870
Size: 73 622 3129 677 4545
Fragments arranged by size:
4545 3129 677 622 73
BamHI G'GATC_C
Cuts at: 5466 6376 6743 5466
Size: 910 367 7769
Fragments arranged by size:
7769 910 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 3351 4044 5761 5980
Size: 325 217 627 245 1319 693 1717 219
Cuts at: 5980 6367 7822 618
Size: 387 1455 1842
Fragments arranged by size:
1842 1717 1455 1319 693 627 387 325
245 219 217
BanII G_rGCy'C
Cuts at: 1391 2505 2979 4304 5651 5692 6120 6365 1391
Size: 1114 474 1325 1347 41 428 245 4072
Fragments arranged by size:
4072 1347 1325 1114 474 428 245 41
BbeI G_GCGC'C
Cuts at: 3355 3355
Size: 9046
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3782 4704 928
Size: 971 1883 922 5270
Fragments arranged by size:
5270 1883 971 922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7237 7271 1903
Size: 34 5300 34 3678
Fragments arranged by size:
5300 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3156 6938 8148 3156
Size: 3782 1210 4054
Fragments arranged by size:
4054 3782 1210
BclI T'GATC_A
Cuts at: 1548 2217 4183 5304 1548
Size: 669 1966 1121 5290
Fragments arranged by size:
5290 1966 1121 669
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5696 7657 136
Size: 107 122 71 631 605 4024 1961 1525
Fragments arranged by size:
4024 1961 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4519 9041 4519
Size: 4522 4524
BlpI GC'TnA_GC
Cuts at: 2641 6337 6463 2641
Size: 3696 126 5224
Fragments arranged by size:
5224 3696 126
Bme1580I G_kGCm'C
Cuts at: 1790 3084 3244 3877 5782 6120 7107 8263 1790
Size: 1294 160 633 1905 338 987 1156 2573
Fragments arranged by size:
2573 1905 1294 1156 987 633 338 160
BmgBI CAC'GTC
Cuts at: 1318 3865 1318
Size: 2547 6499
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4466 5606 5719
Size: 416 53 83 399 24 3049 1140 113
Cuts at: 5719 5776 6777 7735 442
Size: 57 1001 958 1753
Fragments arranged by size:
3049 1753 1140 1001 958 416 399 113
83 57 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9046
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4257 4257
Size: 9046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2607 3262 3388 4046 4082 4134 5684 7706
Size: 1368 655 126 658 36 52 1550 2022
Cuts at: 7706 1239
Size: 2579
Fragments arranged by size:
2579 2022 1550 1368 658 655 126 52
36
Bpu10I CC'TnA_GC
Cuts at: 3258 5958 3258
Size: 2700 6346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2647 4158 7171 8036 8334 2465
Size: 182 1511 3013 865 298 3177
Fragments arranged by size:
3177 3013 1511 865 298 182
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2295 7709 882
Size: 617 796 5414 2219
Fragments arranged by size:
5414 2219 796 617
BsaAI yAC'GTr
Cuts at: 493 1204 4821 6837 493
Size: 711 3617 2016 2702
Fragments arranged by size:
3617 2702 2016 711
BsaBI GATnn'nnATC
Cuts at: 4482 9040 4482
Size: 4558 4488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3352 3825 4628 6853
Size: 53 83 186 936 1819 473 803 2225
Cuts at: 6853 7235 7870 275
Size: 382 635 1451
Fragments arranged by size:
2225 1819 1451 936 803 635 473 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2093 4653 7479 7877 8151 8298 8709 2093
Size: 2560 2826 398 274 147 411 2430
Fragments arranged by size:
2826 2560 2430 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2157 2187 2199 2229 2457
Size: 30 165 30 754 30 12 30 228
Cuts at: 2457 2487 3099 3129 4404 4434 4828 4858 1178
Size: 30 612 30 1275 30 394 30 5366
Fragments arranged by size:
5366 1275 754 612 394 228 165 30
30 30 30 30 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2220 3930 4233 5268 5990 6239 1186
Size: 982 52 1710 303 1035 722 249 3993
Fragments arranged by size:
3993 1710 1035 982 722 303 249 52
BseYI C'CCAG_C
Cuts at: 4593 5385 8249 8745 4593
Size: 792 2864 496 4894
Fragments arranged by size:
4894 2864 792 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3682 4791 5664 1163
Size: 573 1946 1109 873 4545
Fragments arranged by size:
4545 1946 1109 873 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2203 6496 7257 8285 1540
Size: 275 186 202 4293 761 1028 2301
Fragments arranged by size:
4293 2301 1028 761 275 202 186
BsiHKAI G_wGCw'C
Cuts at: 2979 3244 5651 7107 7192 8263 2979
Size: 265 2407 1456 85 1071 3762
Fragments arranged by size:
3762 2407 1456 1071 265 85
BsmI GAATG_Cn'
Cuts at: 2238 5962 6557 6650 2238
Size: 3724 595 93 4634
Fragments arranged by size:
4634 3724 595 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3347 3832 3895 5808 3347
Size: 485 63 1913 6585
Fragments arranged by size:
6585 1913 485 63
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2505 2979 3084 3244 3877 4304 5651
Size: 399 715 474 105 160 633 427 1347
Cuts at: 5651 5692 5782 6120 6365 7107 7192 8263 1391
Size: 41 90 338 245 742 85 1071 2174
Fragments arranged by size:
2174 1347 1071 742 715 633 474 427
399 338 245 160 105 90 85 41
BspEI T'CCGG_A
Cuts at: 8709 8709
Size: 9046
BspHI T'CATG_A
Cuts at: 4230 5473 5706 6309 6935 8665 4230
Size: 1243 233 603 626 1730 4611
Fragments arranged by size:
4611 1730 1243 626 603 233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3367 3612 5304 6401 844
Size: 2523 245 1692 1097 3489
Fragments arranged by size:
3489 2523 1692 1097 245
BsrBI CCG'CTC
Cuts at: 1394 1823 6933 1394
Size: 429 5110 3507
Fragments arranged by size:
5110 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2591 7541 7715 65
Size: 1183 1343 4950 174 1396
Fragments arranged by size:
4950 1396 1343 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 3971 7690 7877 1700
Size: 110 283 1878 3719 187 2869
Fragments arranged by size:
3719 2869 1878 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 3059 3495 4552 96
Size: 2963 436 1057 4590
Fragments arranged by size:
4590 2963 1057 436
BssHII G'CGCG_C
Cuts at: 1925 2076 1925
Size: 151 8895
BssSI C'ACGA_G
Cuts at: 4884 7106 8118 4884
Size: 2222 1012 5812
Fragments arranged by size:
5812 2222 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2405 794
Size: 1611 7435
BstBI TT'CG_AA
Cuts at: 5065 7850 5065
Size: 2785 6261
BstEII G'GTnAC_C
Cuts at: 1184 5314 1184
Size: 4130 4916
BstXI CCAn_nnnn'nTGG
Cuts at: 3307 3559 5417 3307
Size: 252 1858 6936
Fragments arranged by size:
6936 1858 252
BstYI r'GATC_y
Cuts at: 1972 2701 4519 5035 5466 6176 6376 6743 7128
Size: 729 1818 516 431 710 200 367 385
Cuts at: 7128 7145 8597 8683 8695 9041 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1818 1452 729 710 516 431 385
367 346 200 86 17 12
BstZ17I GTA'TAC
Cuts at: 2928 4137 2928
Size: 1209 7837
BtgI C'CryG_G
Cuts at: 513 1065 2004 2190 2996 5715 513
Size: 552 939 186 806 2719 3844
Fragments arranged by size:
3844 2719 939 806 552 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8307
Fragments arranged by size:
8307 683 56
BtsI GCAGTG_nn'
Cuts at: 2120 5275 6562 7356 7376 2120
Size: 3155 1287 794 20 3790
Fragments arranged by size:
3790 3155 1287 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4245 4280 5073 5108 529
Size: 35 3681 35 793 35 4467
Fragments arranged by size:
4467 3681 793 35 35 35
DraI TTT'AAA
Cuts at: 3006 6350 3006
Size: 3344 5702
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4607 4859 8053 809
Size: 507 3291 252 3194 1802
Fragments arranged by size:
3291 3194 1802 507 252
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3561 5192 6493
Size: 54 1425 50 275 18 1731 1631 1301
Cuts at: 6493 7382 9011 8
Size: 889 1629 43
Fragments arranged by size:
1731 1631 1629 1425 1301 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6493 1537
Size: 275 4681 4090
Fragments arranged by size:
4681 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 4203 5123 5383 6129 6976 1353
Size: 707 2143 920 260 746 847 3423
Fragments arranged by size:
3423 2143 920 847 746 707 260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2982 7633 8007 8153 387
Size: 1064 138 1393 4651 374 146 1280
Fragments arranged by size:
4651 1393 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2977 5649 2977
Size: 2672 6374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2607 3262 3388 3443 3500 4046 4082
Size: 393 975 655 126 55 57 546 36
Cuts at: 4082 4134 5104 5364 5684 6234 7109 7706 8493
Size: 52 970 260 320 550 875 597 787
Cuts at: 8493 8761 1239
Size: 268 1524
Fragments arranged by size:
1524 975 970 875 787 655 597 550
546 393 320 268 260 126 57 55
52 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3945 3945
Size: 9046
EcoO109I rG'GnC_Cy
Cuts at: 3453 4209 5443 6116 3453
Size: 756 1234 673 6383
Fragments arranged by size:
6383 1234 756 673
EcoRI G'AATT_C
Cuts at: 4754 5067 6355 6733 4754
Size: 313 1288 378 7067
Fragments arranged by size:
7067 1288 378 313
EcoRV GAT'ATC
Cuts at: 5819 5819
Size: 9046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6198 6198
Size: 9046
FspI TGC'GCA
Cuts at: 7552 7552
Size: 9046
HaeII r_GCGC'y
Cuts at: 1678 3355 5754 8193 1678
Size: 1677 2399 2439 2531
Fragments arranged by size:
2531 2439 2399 1677
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2157 2189 2363 2395 2559
Size: 32 135 32 585 32 174 32 164
Cuts at: 2559 2591 3099 3131 3294 3326 3731 3763 4691
Size: 32 508 32 163 32 405 32 928
Cuts at: 4691 4723 5711 5743 7687 7719 7761 7793 1373
Size: 32 988 32 1944 32 42 32 2626
Fragments arranged by size:
2626 1944 988 928 585 508 405 174
164 163 135 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6390 6636 669
Size: 988 281 4452 246 3079
Fragments arranged by size:
4452 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2109 4395 748
Size: 1361 2286 5399
Fragments arranged by size:
5399 2286 1361
HpaI GTT'AAC
Cuts at: 6636 6636
Size: 9046
KasI G'GCGC_C
Cuts at: 3351 3351
Size: 9046
KpnI G_GTAC'C
Cuts at: 2036 4048 6371 2036
Size: 2012 2323 4711
Fragments arranged by size:
4711 2323 2012
MfeI C'AATT_G
Cuts at: 4977 5241 6645 4977
Size: 264 1404 7378
Fragments arranged by size:
7378 1404 264
MluI A'CGCG_T
Cuts at: 8922 8922
Size: 9046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 2372 2635 2869 3042 4152 4217 5473
Size: 918 149 263 234 173 1110 65 1256
Cuts at: 5473 5754 8160 8344 1305
Size: 281 2406 184 2007
Fragments arranged by size:
2406 2007 1256 1110 918 281 263 234
184 173 149 65
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3563 5194 10
Size: 54 1425 343 1731 1631 3862
Fragments arranged by size:
3862 1731 1631 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3557 3704 4229 4265 4532 5178 5268
Size: 648 2391 147 525 36 267 646 90
Cuts at: 5268 5333 5759 7004 7363 7522 518
Size: 65 426 1245 359 159 2042
Fragments arranged by size:
2391 2042 1245 648 646 525 426 359
267 159 147 90 65 36
MspA1I CmG'CkG
Cuts at: 1606 2192 2722 3348 4593 6462 7139 8287 8532
Size: 586 530 626 1245 1869 677 1148 245
Cuts at: 8532 1606
Size: 2120
Fragments arranged by size:
2120 1869 1245 1148 677 626 586 530
245
NarI GG'CG_CC
Cuts at: 3352 3352
Size: 9046
NcoI C'CATG_G
Cuts at: 513 1065 2004 2996 5715 513
Size: 552 939 992 2719 3844
Fragments arranged by size:
3844 2719 992 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9046
NotI GC'GGCC_GC
Cuts at: 6493 6493
Size: 9046
NspI r_CATG'y
Cuts at: 1132 1229 4814 6404 7949 1132
Size: 97 3585 1590 1545 2229
Fragments arranged by size:
3585 2229 1590 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9046
PciI A'CATG_T
Cuts at: 7945 7945
Size: 9046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4794 1108
Size: 3686 5360
PfoI T'CCnGG_A
Cuts at: 2704 2778 2704
Size: 74 8972
PmeI GTTT'AAAC
Cuts at: 6350 6350
Size: 9046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4404 4436 7115 7147 8628 8660 4404
Size: 32 2679 32 1481 32 4790
Fragments arranged by size:
4790 2679 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3453 5443 3453
Size: 1990 7056
PshAI GACnn'nnGTC
Cuts at: 1613 5166 1613
Size: 3553 5493
PsiI TTA'TAA
Cuts at: 3753 3807 6616 3753
Size: 54 2809 6183
Fragments arranged by size:
6183 2809 54
PspOMI G'GGCC_C
Cuts at: 6116 6116
Size: 9046
PstI C_TGCA'G
Cuts at: 830 2974 3364 4144 5527 6398 830
Size: 2144 390 780 1383 871 3478
Fragments arranged by size:
3478 2144 1383 871 780 390
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9046
PvuII CAG'CTG
Cuts at: 2722 3348 4593 2722
Size: 626 1245 7175
Fragments arranged by size:
7175 1245 626
SacI G_AGCT'C
Cuts at: 2979 5651 2979
Size: 2672 6374
SacII CC_GC'GG
Cuts at: 2193 2193
Size: 9046
SalI G'TCGA_C
Cuts at: 1936 6388 1936
Size: 4452 4594
SanDI GG'GwC_CC
Cuts at: 3453 3453
Size: 9046
SapI GCTCTTCn'nnn_
Cuts at: 5383 5383
Size: 9046
SbfI CC_TGCA'GG
Cuts at: 5527 6398 5527
Size: 871 8175
ScaI AGT'ACT
Cuts at: 4792 7294 9006 4792
Size: 2502 1712 4832
Fragments arranged by size:
4832 2502 1712
SexAI A'CCwGG_T
Cuts at: 2819 4445 2819
Size: 1626 7420
SfcI C'TryA_G
Cuts at: 826 1045 2970 3360 4140 5523 6394 7529 8210
Size: 219 1925 390 780 1383 871 1135 681
Cuts at: 8210 8401 826
Size: 191 1471
Fragments arranged by size:
1925 1471 1383 1135 871 780 681 390
219 191
SfoI GGC'GCC
Cuts at: 3353 3353
Size: 9046
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9046
SmaI CCC'GGG
Cuts at: 1872 5696 6373 1872
Size: 3824 677 4545
Fragments arranged by size:
4545 3824 677
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2480 2662 4173 7150 8051 8313
Size: 197 926 537 182 1511 2977 901 262
Cuts at: 8313 820
Size: 1553
Fragments arranged by size:
2977 1553 1511 926 901 537 262 197
182
SnaBI TAC'GTA
Cuts at: 493 6837 493
Size: 6344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9046
SphI G_CATG'C
Cuts at: 1132 6404 1132
Size: 5272 3774
SspI AAT'ATT
Cuts at: 5 52 2959 5497 6970 5
Size: 47 2907 2538 1473 2081
Fragments arranged by size:
2907 2538 2081 1473 47
StuI AGG'CCT
Cuts at: 1517 3765 4731 1517
Size: 2248 966 5832
Fragments arranged by size:
5832 2248 966
StyI C'CwwG_G
Cuts at: 513 1065 2004 2250 2996 3679 4848 4914 4959
Size: 552 939 246 746 683 1169 66 45
Cuts at: 4959 5164 5715 6154 6485 513
Size: 205 551 439 331 3074
Fragments arranged by size:
3074 1169 939 746 683 552 551 439
331 246 205 66 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2217 2864 4501 4750 5423 5889 6039
Size: 1255 637 647 1637 249 673 466 150
Cuts at: 6039 7073 7090 7243 7428 7873 325
Size: 1034 17 153 185 445 1498
Fragments arranged by size:
1637 1498 1255 1034 673 647 637 466
445 249 185 153 150 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2050 3059 3495
Size: 274 80 33 51 1443 73 1009 436
Cuts at: 3495 4077 4552 4790 7292 9004 96
Size: 582 475 238 2502 1712 138
Fragments arranged by size:
2502 1712 1443 1009 582 475 436 274
238 138 80 73 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2112 2144 2199 2231 2412 2444 2457 2489 3063
Size: 32 55 32 181 32 13 32 574
Cuts at: 3063 3095 4828 4860 4870 4902 9006 9038 2112
Size: 32 1733 32 10 32 4104 32 2120
Fragments arranged by size:
4104 2120 1733 574 181 55 32 32
32 32 32 32 32 32 13 10
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9046
XbaI T'CTAG_A
Cuts at: 4162 6382 4162
Size: 2220 6826
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2220 2517 2817 4038 4128 4983 5171 2220
Size: 297 300 1221 90 855 188 6095
Fragments arranged by size:
6095 1221 855 300 297 188 90
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9046
XmaI C'CCGG_G
Cuts at: 1870 5694 6371 1870
Size: 3824 677 4545
Fragments arranged by size:
4545 3824 677
XmnI GAAnn'nnTTC
Cuts at: 3773 4419 7175 3773
Size: 646 2756 5644
Fragments arranged by size:
5644 2756 646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4629 6854 276
Size: 53 83 186 936 3095 2225 2468
Fragments arranged by size:
3095 2468 2225 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI Bsu36I
DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SfiI SrfI SwaI