Restriction map for
pFN21AE0066
(Circular) (Six-base) MAPSORT of: pFN21AE0066.seq Check: 3406 from: 1 to: 7384
pFN21AE0066 7384 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5194 278
Size: 53 83 186 936 3658 2468
Fragments arranged by size:
3658 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2640 2682 2697 4727 1937
Size: 703 42 15 2030 4594
Fragments arranged by size:
4594 2030 703 42 15
Acc65I G'GTAC_C
Cuts at: 2813 4705 2813
Size: 1892 5492
AclI AA'CG_TT
Cuts at: 1563 5511 5884 1563
Size: 3948 373 3063
Fragments arranged by size:
3948 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2311 2708 3095 3788 4392 4632 5447 6831
Size: 679 397 387 693 604 240 815 1384
Cuts at: 6831 7099 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 815 693 679 604 397 387
268 240
AfeI AGC'GCT
Cuts at: 3821 3821
Size: 7384
AflII C'TTAA_G
Cuts at: 820 1017 3131 820
Size: 197 2114 5073
Fragments arranged by size:
5073 2114 197
AflIII A'CryG_T
Cuts at: 1203 2532 4444 4446 6283 7260 1203
Size: 1329 1912 2 1837 977 1327
Fragments arranged by size:
1912 1837 1329 1327 977 2
AgeI A'CCGG_T
Cuts at: 6215 6215
Size: 7384
AhdI GACnn_n'nnGTC
Cuts at: 6113 6113
Size: 7384
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7384
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7384
AlwNI CAG_nnn'CTG
Cuts at: 2495 4007 4108 4225 5127 6699 2495
Size: 1512 101 117 902 1572 3180
Fragments arranged by size:
3180 1572 1512 902 117 101
ApaI G_GGCC'C
Cuts at: 2174 2174
Size: 7384
ApaLI G'TGCA_C
Cuts at: 5441 6597 5441
Size: 1156 6228
ApoI r'AATT_y
Cuts at: 1493 4693 4919 5071 7180 1493
Size: 3200 226 152 2109 1697
Fragments arranged by size:
3200 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5938 160
Size: 872 4906 1606
Fragments arranged by size:
4906 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7384
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2087 2160 2222 2249 3838 4709
Size: 73 76 68 73 62 27 1589 871
Cuts at: 4709 1870
Size: 4545
Fragments arranged by size:
4545 1589 871 76 73 73 68 62
27
BamHI G'GATC_C
Cuts at: 4714 5081 4714
Size: 367 7017
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2183 2209 2813 4705
Size: 325 217 627 369 27 26 604 1892
Cuts at: 4705 6160 618
Size: 1455 1842
Fragments arranged by size:
1892 1842 1455 627 604 369 325 217
27 26
BanII G_rGCy'C
Cuts at: 1391 2174 2322 3294 4581 4703 1391
Size: 783 148 972 1287 122 4072
Fragments arranged by size:
4072 1287 972 783 148 122
BbeI G_GCGC'C
Cuts at: 2160 2187 2213 2160
Size: 27 26 7331
Fragments arranged by size:
7331 27 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2430 3309 4380 928
Size: 971 531 879 1071 3932
Fragments arranged by size:
3932 1071 971 879 531
BbvCI CC'TCA_GC
Cuts at: 3779 4238 3779
Size: 459 6925
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5575 5609 1903
Size: 34 3638 34 3678
Fragments arranged by size:
3678 3638 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2810 3078 5276 6486 2810
Size: 268 2198 1210 3708
Fragments arranged by size:
3708 2198 1210 268
BclI T'GATC_A
Cuts at: 1548 4477 1548
Size: 2929 4455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2126 2192 4011
Size: 107 122 71 631 605 454 66 1819
Cuts at: 4011 5995 136
Size: 1984 1525
Fragments arranged by size:
1984 1819 1525 631 605 454 122 107
71 66
BglII A'GATC_T
Cuts at: 7379 7379
Size: 7384
BlpI GC'TnA_GC
Cuts at: 2079 2234 2321 4801 2079
Size: 155 87 2480 4662
Fragments arranged by size:
4662 2480 155 87
Bme1580I G_kGCm'C
Cuts at: 1790 2174 3624 5445 6601 1790
Size: 384 1450 1821 1156 2573
Fragments arranged by size:
2573 1821 1450 1156 384
BmgBI CAC'GTC
Cuts at: 1318 2644 1318
Size: 1326 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4289 4630 5115
Size: 416 53 83 399 24 2872 341 485
Cuts at: 5115 6073 442
Size: 958 1753
Fragments arranged by size:
2872 1753 958 485 416 399 341 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3449 3894 3984 4117 6044 1239
Size: 2210 445 90 133 1927 2579
Fragments arranged by size:
2579 2210 1927 445 133 90
Bpu10I CC'TnA_GC
Cuts at: 2615 3102 3779 4238 2615
Size: 487 677 459 5761
Fragments arranged by size:
5761 677 487 459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 3442 3915 5509 6374 6672 2369
Size: 1073 473 1594 865 298 3081
Fragments arranged by size:
3081 1594 1073 865 473 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4002 6047 882
Size: 617 2503 2045 2219
Fragments arranged by size:
2503 2219 2045 617
BsaAI yAC'GTr
Cuts at: 493 1204 4447 5175 493
Size: 711 3243 728 2702
Fragments arranged by size:
3243 2702 728 711
BsaBI GATnn'nnATC
Cuts at: 7378 7378
Size: 7384
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2157 2184 2210
Size: 53 83 186 936 476 148 27 26
Cuts at: 2210 5191 5573 6208 275
Size: 2981 382 635 1451
Fragments arranged by size:
2981 1451 936 635 476 382 186 148
83 53 27 26
BsaWI w'CCGG_w
Cuts at: 5817 6215 6489 6636 7047 5817
Size: 398 274 147 411 6154
Fragments arranged by size:
6154 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7159
Fragments arranged by size:
7159 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 2444 4120 4534 1186
Size: 1144 114 1676 414 4036
Fragments arranged by size:
4036 1676 1144 414 114
BseYI C'CCAG_C
Cuts at: 2106 2195 2305 2331 4080 4181 6587 7083 2106
Size: 89 110 26 1749 101 2406 496 2407
Fragments arranged by size:
2407 2406 1749 496 110 101 89 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4094 1163
Size: 573 2358 4453
Fragments arranged by size:
4453 2358 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 4834 5595 6623 1540
Size: 275 186 120 2713 761 1028 2301
Fragments arranged by size:
2713 2301 1028 761 275 186 120
BsiHKAI G_wGCw'C
Cuts at: 2105 3294 3885 5445 5530 6601 2105
Size: 1189 591 1560 85 1071 2888
Fragments arranged by size:
2888 1560 1189 1071 591 85
BsmI GAATG_Cn'
Cuts at: 4895 4988 4895
Size: 93 7291
BsmBI CGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 7384
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2174 2322 3294 3624 3885 4581
Size: 399 315 69 148 972 330 261 696
Cuts at: 4581 4703 5445 5530 6601 1391
Size: 122 742 85 1071 2174
Fragments arranged by size:
2174 1071 972 742 696 399 330 315
261 148 122 85 69
BspEI T'CCGG_A
Cuts at: 7047 7047
Size: 7384
BspHI T'CATG_A
Cuts at: 5273 7003 5273
Size: 1730 5654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 4739 844
Size: 1631 2264 3489
Fragments arranged by size:
3489 2264 1631
BsrBI CCG'CTC
Cuts at: 1394 1823 2154 5271 1394
Size: 429 331 3117 3507
Fragments arranged by size:
3507 3117 429 331
BsrDI GCAATG_nn'
Cuts at: 65 1248 3527 4359 5879 6053 65
Size: 1183 2279 832 1520 174 1396
Fragments arranged by size:
2279 1520 1396 1183 832 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2186 4094 6028 6215 1700
Size: 110 376 1908 1934 187 2869
Fragments arranged by size:
2869 1934 1908 376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7384
BssHII G'CGCG_C
Cuts at: 1925 2406 2455 1925
Size: 481 49 6854
Fragments arranged by size:
6854 481 49
BssSI C'ACGA_G
Cuts at: 5444 6456 5444
Size: 1012 6372
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7384
BstBI TT'CG_AA
Cuts at: 6188 6188
Size: 7384
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7384
BstXI CCAn_nnnn'nTGG
Cuts at: 2810 4141 2810
Size: 1331 6053
BstYI r'GATC_y
Cuts at: 1972 3106 4287 4714 5081 5466 5483 6935 7021
Size: 1134 1181 427 367 385 17 1452 86
Cuts at: 7021 7033 7379 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1181 1134 427 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2683 2683
Size: 7384
Bsu36I CC'TnA_GG
Cuts at: 2660 2885 4089 4101 4671 2660
Size: 225 1204 12 570 5373
Fragments arranged by size:
5373 1204 570 225 12
BtgI C'CryG_G
Cuts at: 513 1065 2377 513
Size: 552 1312 5520
Fragments arranged by size:
5520 1312 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2162 4171 4195 4691 487
Size: 683 56 936 2009 24 496 3180
Fragments arranged by size:
3180 2009 936 683 496 56 24
BtsI GCAGTG_nn'
Cuts at: 2137 4057 4900 5694 5714 2137
Size: 1920 843 794 20 3807
Fragments arranged by size:
3807 1920 843 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7384
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7349
DraI TTT'AAA
Cuts at: 4688 4688
Size: 7384
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2014 4391 6391 809
Size: 507 698 2377 2000 1802
Fragments arranged by size:
2377 2000 1802 698 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 4831 5720
Size: 54 1425 50 275 18 288 2713 889
Cuts at: 5720 7349 8
Size: 1629 43
Fragments arranged by size:
2713 1629 1425 889 288 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2118 4831 1537
Size: 275 306 2713 4090
Fragments arranged by size:
4090 2713 306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5314 1353
Size: 3961 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 2056 5971 6345 6491 387
Size: 1064 138 450 17 3915 374 146 1280
Fragments arranged by size:
3915 1280 1064 450 374 146 138 17
EcoICRI GAG'CTC
Cuts at: 3292 3292
Size: 7384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2311 2708 3095 3449 3788 3894 3984
Size: 393 679 397 387 354 339 106 90
Cuts at: 3984 4117 4392 4632 5447 6044 6831 7099 1239
Size: 133 275 240 815 597 787 268 1524
Fragments arranged by size:
1524 815 787 679 597 397 393 387
354 339 275 268 240 133 106 90
EcoNI CCTnn'n_nnAGG
Cuts at: 3467 3467
Size: 7384
EcoO109I rG'GnC_Cy
Cuts at: 2170 2389 3715 3772 4004 2170
Size: 219 1326 57 232 5550
Fragments arranged by size:
5550 1326 232 219 57
EcoRI G'AATT_C
Cuts at: 4693 5071 4693
Size: 378 7006
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3337 3436 4598 3337
Size: 99 1162 6123
Fragments arranged by size:
6123 1162 99
FseI GG_CCGG'CC
Cuts at: 4098 4098
Size: 7384
FspI TGC'GCA
Cuts at: 5890 5890
Size: 7384
HaeII r_GCGC'y
Cuts at: 1678 2068 2160 2187 2213 2417 3370 3712 3823
Size: 390 92 27 26 204 953 342 111
Cuts at: 3823 6531 1678
Size: 2708 2531
Fragments arranged by size:
2708 2531 953 390 342 204 111 92
27 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2969 3001 4654 4686 6025
Size: 32 135 32 1397 32 1653 32 1339
Cuts at: 6025 6057 6099 6131 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1653 1397 1339 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2641 4728 4974 669
Size: 988 281 703 2087 246 3079
Fragments arranged by size:
3079 2087 988 703 281 246
HindIII A'AGCT_T
Cuts at: 748 4325 4483 748
Size: 3577 158 3649
Fragments arranged by size:
3649 3577 158
HpaI GTT'AAC
Cuts at: 4974 4974
Size: 7384
KasI G'GCGC_C
Cuts at: 2156 2183 2209 2156
Size: 27 26 7331
Fragments arranged by size:
7331 27 26
KpnI G_GTAC'C
Cuts at: 2817 4709 2817
Size: 1892 5492
MfeI C'AATT_G
Cuts at: 4983 4983
Size: 7384
MluI A'CGCG_T
Cuts at: 7260 7260
Size: 7384
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2842 6498 6682 1305
Size: 1537 3656 184 2007
Fragments arranged by size:
3656 2007 1537 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5562
Fragments arranged by size:
5562 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2822 3443 3809 3860 5342 5701 5860
Size: 648 1656 621 366 51 1482 359 159
Cuts at: 5860 518
Size: 2042
Fragments arranged by size:
2042 1656 1482 648 621 366 359 159
51
MspA1I CmG'CkG
Cuts at: 1606 2266 2331 2379 3787 4246 4294 4800 5477
Size: 660 65 48 1408 459 48 506 677
Cuts at: 5477 6625 6870 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1408 1148 677 660 506 459 245
65 48 48
NaeI GCC'GGC
Cuts at: 2188 4096 2188
Size: 1908 5476
NarI GG'CG_CC
Cuts at: 2157 2184 2210 2157
Size: 27 26 7331
Fragments arranged by size:
7331 27 26
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6832
NdeI CA'TA_TG
Cuts at: 387 3987 387
Size: 3600 3784
NgoMIV G'CCGG_C
Cuts at: 2186 4094 2186
Size: 1908 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7384
NotI GC'GGCC_GC
Cuts at: 4831 4831
Size: 7384
NspI r_CATG'y
Cuts at: 1132 1229 2536 4346 4402 4742 6287 1132
Size: 97 1307 1810 56 340 1545 2229
Fragments arranged by size:
2229 1810 1545 1307 340 97 56
PasI CC'CwG_GG
Cuts at: 1348 3757 4264 1348
Size: 2409 507 4468
Fragments arranged by size:
4468 2409 507
PciI A'CATG_T
Cuts at: 2532 6283 2532
Size: 3751 3633
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4007 4043 4629 1108
Size: 2899 36 586 3863
Fragments arranged by size:
3863 2899 586 36
PfoI T'CCnGG_A
Cuts at: 2829 3268 2829
Size: 439 6945
PmeI GTTT'AAAC
Cuts at: 4688 4688
Size: 7384
PmlI CAC'GTG
Cuts at: 4447 4447
Size: 7384
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5453 5485 6966 6998 5453
Size: 32 1481 32 5839
Fragments arranged by size:
5839 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3715 3772 3715
Size: 57 7327
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7384
PsiI TTA'TAA
Cuts at: 4954 4954
Size: 7384
PspOMI G'GGCC_C
Cuts at: 2170 2170
Size: 7384
PstI C_TGCA'G
Cuts at: 830 4352 4736 830
Size: 3522 384 3478
Fragments arranged by size:
3522 3478 384
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7384
PvuII CAG'CTG
Cuts at: 2266 2331 3787 4246 4294 2266
Size: 65 1456 459 48 5356
Fragments arranged by size:
5356 1456 459 65 48
SacI G_AGCT'C
Cuts at: 3294 3294
Size: 7384
SacII CC_GC'GG
Cuts at: 2380 2380
Size: 7384
SalI G'TCGA_C
Cuts at: 1936 2639 4726 1936
Size: 703 2087 4594
Fragments arranged by size:
4594 2087 703
SbfI CC_TGCA'GG
Cuts at: 4736 4736
Size: 7384
ScaI AGT'ACT
Cuts at: 5632 7344 5632
Size: 1712 5672
SfcI C'TryA_G
Cuts at: 826 1045 2580 4348 4732 5867 6548 6739 826
Size: 219 1535 1768 384 1135 681 191 1471
Fragments arranged by size:
1768 1535 1471 1135 681 384 219 191
SfoI GGC'GCC
Cuts at: 2158 2185 2211 2158
Size: 27 26 7331
Fragments arranged by size:
7331 27 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7384
SgrAI Cr'CCGG_yG
Cuts at: 1700 2186 1700
Size: 486 6898
SmaI CCC'GGG
Cuts at: 1872 2021 2089 2162 2224 2251 4711 1872
Size: 149 68 73 62 27 2460 4545
Fragments arranged by size:
4545 2460 149 73 68 62 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 3131 3421 3930 5488 6389
Size: 197 926 441 747 290 509 1558 901
Cuts at: 6389 6651 820
Size: 262 1553
Fragments arranged by size:
1558 1553 926 901 747 509 441 290
262 197
SnaBI TAC'GTA
Cuts at: 493 5175 493
Size: 4682 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7384
SphI G_CATG'C
Cuts at: 1132 4346 4742 1132
Size: 3214 396 3774
Fragments arranged by size:
3774 3214 396
SrfI GCCC'GGGC
Cuts at: 2251 2251
Size: 7384
SspI AAT'ATT
Cuts at: 5 52 5308 5
Size: 47 5256 2081
Fragments arranged by size:
5256 2081 47
StuI AGG'CCT
Cuts at: 1517 3907 4669 1517
Size: 2390 762 4232
Fragments arranged by size:
4232 2390 762
StyI C'CwwG_G
Cuts at: 513 1065 3405 4582 4823 513
Size: 552 2340 1177 241 3074
Fragments arranged by size:
3074 2340 1177 552 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2120 2624 4232 5411 5428 5581 5766
Size: 1255 540 504 1608 1179 17 153 185
Cuts at: 5766 6211 325
Size: 445 1498
Fragments arranged by size:
1608 1498 1255 1179 540 504 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3540 5630 7342
Size: 274 80 33 51 1443 1563 2090 1712
Cuts at: 7342 96
Size: 138
Fragments arranged by size:
2090 1712 1563 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2656 2688 3737 3769 7344 7376 2656
Size: 32 1049 32 3575 32 2664
Fragments arranged by size:
3575 2664 1049 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7384
XbaI T'CTAG_A
Cuts at: 4720 4720
Size: 7384
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3509 3614 3509
Size: 105 7279
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7384
XmaI C'CCGG_G
Cuts at: 1870 2019 2087 2160 2222 2249 4709 1870
Size: 149 68 73 62 27 2460 4545
Fragments arranged by size:
4545 2460 149 73 68 62 27
XmnI GAAnn'nnTTC
Cuts at: 5513 5513
Size: 7384
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5192 276
Size: 53 83 186 936 3658 2468
Fragments arranged by size:
3658 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
DraIII EcoRV FspAI NruI NsiI PacI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SwaI