Restriction map for
pFN21AE0062
(Circular) (Six-base) MAPSORT of: pFN21AE0062.seq Check: 1272 from: 1 to: 6607
pFN21AE0062 6607 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4417 278
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2634 3002 3950 1937
Size: 697 368 948 4594
Fragments arranged by size:
4594 948 697 368
Acc65I G'GTAC_C
Cuts at: 3928 3928
Size: 6607
AclI AA'CG_TT
Cuts at: 1563 4734 5107 1563
Size: 3171 373 3063
Fragments arranged by size:
3171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 3759 4670 6054 6322 1632
Size: 1056 1071 911 1384 268 1917
Fragments arranged by size:
1917 1384 1071 1056 911 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6410
AflIII A'CryG_T
Cuts at: 1203 2259 2302 2472 3175 5506 6483 1203
Size: 1056 43 170 703 2331 977 1327
Fragments arranged by size:
2331 1327 1056 977 703 170 43
AgeI A'CCGG_T
Cuts at: 5438 5438
Size: 6607
AhdI GACnn_n'nnGTC
Cuts at: 5336 5336
Size: 6607
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6607
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6607
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3795 3827 3795
Size: 32 6575
AlwNI CAG_nnn'CTG
Cuts at: 2742 2860 4350 5922 2742
Size: 118 1490 1572 3427
Fragments arranged by size:
3427 1572 1490 118
ApaLI G'TGCA_C
Cuts at: 4664 5820 4664
Size: 1156 5451
ApoI r'AATT_y
Cuts at: 1493 3230 3853 3916 4142 4294 6403 1493
Size: 1737 623 63 226 152 2109 1697
Fragments arranged by size:
2109 1737 1697 623 226 152 63
AseI AT'TA_AT
Cuts at: 160 1032 5161 160
Size: 872 4129 1606
Fragments arranged by size:
4129 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2615 3932 1870
Size: 73 153 519 1317 4545
Fragments arranged by size:
4545 1317 519 153 73
BamHI G'GATC_C
Cuts at: 2042 3208 3937 4304 2042
Size: 1166 729 367 4345
Fragments arranged by size:
4345 1166 729 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2214 3928 5383 618
Size: 325 217 627 427 1714 1455 1842
Fragments arranged by size:
1842 1714 1455 627 427 325 217
BanII G_rGCy'C
Cuts at: 1391 2628 3542 3926 1391
Size: 1237 914 384 4072
Fragments arranged by size:
4072 1237 914 384
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2365 928
Size: 971 466 5170
Fragments arranged by size:
5170 971 466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3093 3127 4798 4832 1903
Size: 34 1156 34 1671 34 3678
Fragments arranged by size:
3678 1671 1156 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2487 4499 5709 2487
Size: 2012 1210 3385
Fragments arranged by size:
3385 2012 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6607
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5218 136
Size: 107 122 71 631 605 3546 1525
Fragments arranged by size:
3546 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6602 6602
Size: 6607
BlpI GC'TnA_GC
Cuts at: 4024 4024
Size: 6607
Bme1580I G_kGCm'C
Cuts at: 1790 4668 5824 1790
Size: 2878 1156 2573
Fragments arranged by size:
2878 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6607
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2631 4338 5296
Size: 416 53 83 399 24 1214 1707 958
Cuts at: 5296 442
Size: 1753
Fragments arranged by size:
1753 1707 1214 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2205 5267 1239
Size: 966 3062 2579
Fragments arranged by size:
3062 2579 966
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4732 5597 5895 4732
Size: 865 298 5444
Fragments arranged by size:
5444 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5270 882
Size: 617 3771 2219
Fragments arranged by size:
3771 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4398 493
Size: 711 3194 2702
Fragments arranged by size:
3194 2702 711
BsaBI GATnn'nnATC
Cuts at: 2433 6601 2433
Size: 4168 2439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2821 4414 4796 5431
Size: 53 83 186 936 1288 1593 382 635
Cuts at: 5431 275
Size: 1451
Fragments arranged by size:
1593 1451 1288 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2205 5040 5438 5712 5859 6270 2205
Size: 2835 398 274 147 411 2542
Fragments arranged by size:
2835 2542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3795 3825 1178
Size: 30 165 30 2392 30 3960
Fragments arranged by size:
3960 2392 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2151 2202 1186
Size: 965 51 5591
Fragments arranged by size:
5591 965 51
BseYI C'CCAG_C
Cuts at: 3892 5810 6306 3892
Size: 1918 496 4193
Fragments arranged by size:
4193 1918 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6034
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4057 4818 5846 1540
Size: 275 186 2056 761 1028 2301
Fragments arranged by size:
2301 2056 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2811 3259 3542 4668 4753 5824 2811
Size: 448 283 1126 85 1071 3594
Fragments arranged by size:
3594 1126 1071 448 283 85
BsmI GAATG_Cn'
Cuts at: 4118 4211 4118
Size: 93 6514
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2628 2811 3259 3542 3926 4668 4753
Size: 399 838 183 448 283 384 742 85
Cuts at: 4753 5824 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 838 742 448 399 384 283
183 85
BspEI T'CCGG_A
Cuts at: 6270 6270
Size: 6607
BspHI T'CATG_A
Cuts at: 2524 3217 4496 6226 2524
Size: 693 1279 1730 2905
Fragments arranged by size:
2905 1730 1279 693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3962 844
Size: 3118 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4494 1394
Size: 429 2671 3507
Fragments arranged by size:
3507 2671 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5102 5276 65
Size: 1183 3854 174 1396
Fragments arranged by size:
3854 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5251 5438 1700
Size: 110 3441 187 2869
Fragments arranged by size:
3441 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6607
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6607
BssSI C'ACGA_G
Cuts at: 4667 5679 4667
Size: 1012 5595
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6607
BstBI TT'CG_AA
Cuts at: 3634 5411 3634
Size: 1777 4830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6607
BstYI r'GATC_y
Cuts at: 1972 2042 2456 3208 3937 4304 4689 4706 6158
Size: 70 414 752 729 367 385 17 1452
Cuts at: 6158 6244 6256 6602 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 752 729 414 385 367 346
86 70 17 12
BstZ17I GTA'TAC
Cuts at: 2635 2635
Size: 6607
BtgI C'CryG_G
Cuts at: 513 1065 3090 513
Size: 552 2025 4030
Fragments arranged by size:
4030 2025 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5868
Fragments arranged by size:
5868 683 56
BtsI GCAGTG_nn'
Cuts at: 2761 2809 4123 4917 4937 2761
Size: 48 1314 794 20 4431
Fragments arranged by size:
4431 1314 794 48 20
ClaI AT'CG_AT
Cuts at: 1554 3324 1554
Size: 1770 4837
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6572
DraI TTT'AAA
Cuts at: 3911 3911
Size: 6607
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5614 809
Size: 507 4298 1802
Fragments arranged by size:
4298 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4054 4943 6572
Size: 54 1425 50 275 18 2224 889 1629
Cuts at: 6572 8
Size: 43
Fragments arranged by size:
2224 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4054 1537
Size: 275 2242 4090
Fragments arranged by size:
4090 2242 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 3778 4537 1353
Size: 731 1694 759 3423
Fragments arranged by size:
3423 1694 759 731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5194 5568 5714 387
Size: 1064 138 3605 374 146 1280
Fragments arranged by size:
3605 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3540 3540
Size: 6607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2205 2688 3759 4670 5267 6054 6322
Size: 393 573 483 1071 911 597 787 268
Cuts at: 6322 1239
Size: 1524
Fragments arranged by size:
1524 1071 911 787 597 573 483 393
268
EcoRI G'AATT_C
Cuts at: 3916 4294 3916
Size: 378 6229
EcoRV GAT'ATC
Cuts at: 2920 3641 2920
Size: 721 5886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3326 3326
Size: 6607
FspI TGC'GCA
Cuts at: 5113 5113
Size: 6607
HaeII r_GCGC'y
Cuts at: 1678 5754 1678
Size: 4076 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3165 3197 5248 5280 5322
Size: 32 135 32 1593 32 2051 32 42
Cuts at: 5322 5354 1373
Size: 32 2626
Fragments arranged by size:
2626 2051 1593 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3951 4197 669
Size: 988 281 2013 246 3079
Fragments arranged by size:
3079 2013 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6607
HpaI GTT'AAC
Cuts at: 4197 4197
Size: 6607
KpnI G_GTAC'C
Cuts at: 3932 3932
Size: 6607
MfeI C'AATT_G
Cuts at: 4206 4206
Size: 6607
MluI A'CGCG_T
Cuts at: 6483 6483
Size: 6607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5721 5905 1305
Size: 4416 184 2007
Fragments arranged by size:
4416 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4785
Fragments arranged by size:
4785 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2523 3366 4565 4924 5083 518
Size: 648 1357 843 1199 359 159 2042
Fragments arranged by size:
2042 1357 1199 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2684 2860 3155 3241 4023 4700 5848 6093
Size: 1078 176 295 86 782 677 1148 245
Cuts at: 6093 1606
Size: 2120
Fragments arranged by size:
2120 1148 1078 782 677 295 245 176
86
NcoI C'CATG_G
Cuts at: 513 1065 3090 513
Size: 552 2025 4030
Fragments arranged by size:
4030 2025 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6607
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6607
NotI GC'GGCC_GC
Cuts at: 4054 4054
Size: 6607
NspI r_CATG'y
Cuts at: 1132 1229 2263 2306 2407 2446 2476 2984 3179
Size: 97 1034 43 101 39 30 508 195
Cuts at: 3179 3350 3965 5510 1132
Size: 171 615 1545 2229
Fragments arranged by size:
2229 1545 1034 615 508 195 171 101
97 43 39 30
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6607
PciI A'CATG_T
Cuts at: 2259 2302 2472 3175 5506 2259
Size: 43 170 703 2331 3360
Fragments arranged by size:
3360 2331 703 170 43
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2860 3749 1108
Size: 1752 889 3966
Fragments arranged by size:
3966 1752 889
PfoI T'CCnGG_A
Cuts at: 3204 3204
Size: 6607
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3795 3827 4676 4708 6189 6221 3795
Size: 32 849 32 1481 32 4181
Fragments arranged by size:
4181 1481 849 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6607
PsiI TTA'TAA
Cuts at: 4177 4177
Size: 6607
PspXI vC'TCGA_Gb
Cuts at: 2096 2096
Size: 6607
PstI C_TGCA'G
Cuts at: 830 2683 3959 830
Size: 1853 1276 3478
Fragments arranged by size:
3478 1853 1276
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
PvuII CAG'CTG
Cuts at: 2684 2860 3155 3241 2684
Size: 176 295 86 6050
Fragments arranged by size:
6050 295 176 86
SacI G_AGCT'C
Cuts at: 3542 3542
Size: 6607
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SbfI CC_TGCA'GG
Cuts at: 3959 3959
Size: 6607
ScaI AGT'ACT
Cuts at: 2574 4855 6567 2574
Size: 2281 1712 2614
Fragments arranged by size:
2614 2281 1712
SfcI C'TryA_G
Cuts at: 826 1045 2679 3955 5090 5771 5962 826
Size: 219 1634 1276 1135 681 191 1471
Fragments arranged by size:
1634 1471 1276 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6607
SmaI CCC'GGG
Cuts at: 1872 3934 1872
Size: 2062 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 2615 4711 5612 5874 820
Size: 197 926 153 519 2096 901 262 1553
Fragments arranged by size:
2096 1553 926 901 519 262 197 153
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 3725 152
Size: 3573 3034
SphI G_CATG'C
Cuts at: 1132 2446 3350 3965 1132
Size: 1314 904 615 3774
Fragments arranged by size:
3774 1314 904 615
SspI AAT'ATT
Cuts at: 5 52 2561 3193 4531 5
Size: 47 2509 632 1338 2081
Fragments arranged by size:
2509 2081 1338 632 47
StuI AGG'CCT
Cuts at: 1517 3578 1517
Size: 2061 4546
StyI C'CwwG_G
Cuts at: 513 1065 2871 3090 3573 3645 3903 4046 513
Size: 552 1806 219 483 72 258 143 3074
Fragments arranged by size:
3074 1806 552 483 258 219 143 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4634 4651 4804 4989 5434 325
Size: 1255 3054 17 153 185 445 1498
Fragments arranged by size:
3054 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2572 4853 6565
Size: 274 80 33 51 1443 595 2281 1712
Cuts at: 6565 96
Size: 138
Fragments arranged by size:
2281 1712 1443 595 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6567 6599 6567
Size: 32 6575
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6607
XbaI T'CTAG_A
Cuts at: 3943 3943
Size: 6607
XhoI C'TCGA_G
Cuts at: 1943 2096 2615 1943
Size: 153 519 5935
Fragments arranged by size:
5935 519 153
XmaI C'CCGG_G
Cuts at: 1870 3932 1870
Size: 2062 4545
XmnI GAAnn'nnTTC
Cuts at: 3273 4736 3273
Size: 1463 5144
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4415 276
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI