Restriction map for
pFN21AE0060
(Circular) (Six-base) MAPSORT of: pFN21AE0060.seq Check: 8390 from: 1 to: 6430
pFN21AE0060 6430 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3673 3673
Size: 6430
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 1937
Size: 1836 4594
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3222 3579 4493 5877 6145 1632
Size: 1590 357 914 1384 268 1917
Fragments arranged by size:
1917 1590 1384 914 357 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 2671 2694 5329 6306 1203
Size: 1468 23 2635 977 1327
Fragments arranged by size:
2635 1468 1327 977 23
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6430
AlwNI CAG_nnn'CTG
Cuts at: 2088 3052 4173 5745 2088
Size: 964 1121 1572 2773
Fragments arranged by size:
2773 1572 1121 964
ApaI G_GGCC'C
Cuts at: 2158 2158
Size: 6430
ApaLI G'TGCA_C
Cuts at: 4487 5643 4487
Size: 1156 5274
ApoI r'AATT_y
Cuts at: 1493 2013 2567 3739 3965 4117 6226 1493
Size: 520 554 1172 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 554 520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 2782 3755 1870
Size: 73 208 631 973 4545
Fragments arranged by size:
4545 973 631 208 73
AvrII C'CTAG_G
Cuts at: 3068 3068
Size: 6430
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3751 5206 618
Size: 325 217 627 1964 1455 1842
Fragments arranged by size:
1964 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2158 2992 3749 1391
Size: 767 834 757 4072
Fragments arranged by size:
4072 834 767 757
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2315 2743 3552 928
Size: 971 416 428 809 3806
Fragments arranged by size:
3806 971 809 428 416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4621 4655 1903
Size: 34 2684 34 3678
Fragments arranged by size:
3678 2684 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4322 5532 3377
Size: 945 1210 4275
Fragments arranged by size:
4275 1210 945
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5041 136
Size: 107 122 71 631 605 3369 1525
Fragments arranged by size:
3369 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3127 6425 3127
Size: 3298 3132
BlpI GC'TnA_GC
Cuts at: 3847 3847
Size: 6430
Bme1580I G_kGCm'C
Cuts at: 1790 2158 4491 5647 1790
Size: 368 2333 1156 2573
Fragments arranged by size:
2573 2333 1156 368
BmgBI CAC'GTC
Cuts at: 1318 2674 1318
Size: 1356 5074
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2534 4161 5119
Size: 416 53 83 399 24 1117 1627 958
Cuts at: 5119 442
Size: 1753
Fragments arranged by size:
1753 1627 1117 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3099 3412 3658 5090 1239
Size: 1860 313 246 1432 2579
Fragments arranged by size:
2579 1860 1432 313 246
Bpu10I CC'TnA_GC
Cuts at: 3099 3099
Size: 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2206 2561 4555 5420 5718 2206
Size: 355 1994 865 298 2918
Fragments arranged by size:
2918 1994 865 355 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2852 5093 882
Size: 617 1353 2241 2219
Fragments arranged by size:
2241 2219 1353 617
BsaAI yAC'GTr
Cuts at: 493 1204 4221 493
Size: 711 3017 2702
Fragments arranged by size:
3017 2702 711
BsaBI GATnn'nnATC
Cuts at: 3281 6424 3281
Size: 3143 3287
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4237 4619 5254 275
Size: 53 83 186 936 2704 382 635 1451
Fragments arranged by size:
2704 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2094 2443 3707 4863 5261 5535 5682 6093 2094
Size: 349 1264 1156 398 274 147 411 2431
Fragments arranged by size:
2431 1264 1156 411 398 349 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2799 2829 3095 3125 3410
Size: 30 165 30 1396 30 266 30 285
Cuts at: 3410 3440 3505 3535 1178
Size: 30 65 30 4073
Fragments arranged by size:
4073 1396 285 266 165 65 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 2820 2910 1186
Size: 1147 487 90 4706
Fragments arranged by size:
4706 1147 487 90
BseYI C'CCAG_C
Cuts at: 2059 3230 3259 5633 6129 2059
Size: 1171 29 2374 496 2360
Fragments arranged by size:
2374 2360 1171 496 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5857
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3880 4641 5669 1540
Size: 275 186 1879 761 1028 2301
Fragments arranged by size:
2301 1879 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4491 4576 5647 4491
Size: 85 1071 5274
Fragments arranged by size:
5274 1071 85
BsmI GAATG_Cn'
Cuts at: 3941 4034 3941
Size: 93 6337
BsmBI CGTCTCn'nnnn_
Cuts at: 2620 2620
Size: 6430
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2992 3749 4491 4576 5647 1391
Size: 399 368 834 757 742 85 1071 2174
Fragments arranged by size:
2174 1071 834 757 742 399 368 85
BspEI T'CCGG_A
Cuts at: 2094 2443 6093 2094
Size: 349 3650 2431
Fragments arranged by size:
3650 2431 349
BspHI T'CATG_A
Cuts at: 4319 6049 4319
Size: 1730 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3673 3785 844
Size: 2829 112 3489
Fragments arranged by size:
3489 2829 112
BsrBI CCG'CTC
Cuts at: 1394 1823 2136 2257 4317 1394
Size: 429 313 121 2060 3507
Fragments arranged by size:
3507 2060 429 313 121
BsrDI GCAATG_nn'
Cuts at: 65 1248 3195 3460 3688 4925 5099 65
Size: 1183 1947 265 228 1237 174 1396
Fragments arranged by size:
1947 1396 1237 1183 265 228 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2242 3515 5074 5261 1700
Size: 110 432 1273 1559 187 2869
Fragments arranged by size:
2869 1559 1273 432 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 4490 5502 4490
Size: 1012 5418
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3197 794
Size: 2403 4027
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 2401 1184
Size: 1217 5213
BstYI r'GATC_y
Cuts at: 1972 3082 3127 3760 4127 4512 4529 5981 6067
Size: 1110 45 633 367 385 17 1452 86
Cuts at: 6067 6079 6425 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1110 633 385 367 346 86
45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2811 3377 3462 513
Size: 552 1746 566 85 3481
Fragments arranged by size:
3481 1746 566 552 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2401 487
Size: 683 56 1175 4516
Fragments arranged by size:
4516 1175 683 56
BtsI GCAGTG_nn'
Cuts at: 3946 4740 4760 3946
Size: 794 20 5616
Fragments arranged by size:
5616 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 2565 3734 2565
Size: 1169 5261
DraIII CAC_nnn'GTG
Cuts at: 2476 2476
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2120 2381 5437 809
Size: 507 804 261 3056 1802
Fragments arranged by size:
3056 1802 804 507 261
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3465 3877 4766
Size: 54 1425 50 275 18 1635 412 889
Cuts at: 4766 6395 8
Size: 1629 43
Fragments arranged by size:
1635 1629 1425 889 412 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3877 1537
Size: 275 2065 4090
Fragments arranged by size:
4090 2065 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2026 3394 3598 3614 4360 1353
Size: 673 1368 204 16 746 3423
Fragments arranged by size:
3423 1368 746 673 204 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5017 5391 5537 387
Size: 1064 138 3428 374 146 1280
Fragments arranged by size:
3428 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3099 3222 3412 3579 3658 4493 5090
Size: 393 1467 123 190 167 79 835 597
Cuts at: 5090 5877 6145 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1467 835 787 597 393 268 190
167 123 79
EcoO109I rG'GnC_Cy
Cuts at: 2085 2374 3645 2085
Size: 289 1271 4870
Fragments arranged by size:
4870 1271 289
EcoRI G'AATT_C
Cuts at: 2013 3739 4117 2013
Size: 1726 378 4326
Fragments arranged by size:
4326 1726 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3643 3643
Size: 6430
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 2222 5577 1678
Size: 544 3355 2531
Fragments arranged by size:
3355 2531 544
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2470 2502 2516 2548 2799
Size: 32 135 32 898 32 14 32 251
Cuts at: 2799 2831 5071 5103 5145 5177 1373
Size: 32 2240 32 42 32 2626
Fragments arranged by size:
2626 2240 898 251 135 42 32 32
32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2649 3327 3774 4020 669
Size: 988 281 711 678 447 246 3079
Fragments arranged by size:
3079 988 711 678 447 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2083 2205 2220 5544 5728 1305
Size: 778 122 15 3324 184 2007
Fragments arranged by size:
3324 2007 778 184 122 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3467 10
Size: 54 1425 343 1635 2973
Fragments arranged by size:
2973 1635 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4388 4747 4906 518
Size: 648 3222 359 159 2042
Fragments arranged by size:
3222 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3379 3678 3846 4523 5671 5916 1606
Size: 1773 299 168 677 1148 245 2120
Fragments arranged by size:
2120 1773 1148 677 299 245 168
NcoI C'CATG_G
Cuts at: 513 1065 2811 3462 513
Size: 552 1746 651 3481
Fragments arranged by size:
3481 1746 651 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 2698 3788 5333 1132
Size: 97 1469 1090 1545 2229
Fragments arranged by size:
2229 1545 1469 1090 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6430
PciI A'CATG_T
Cuts at: 2694 5329 2694
Size: 2635 3795
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 1108
Size: 1137 5293
PfoI T'CCnGG_A
Cuts at: 3076 3076
Size: 6430
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2361 2393 4499 4531 6012 6044 2361
Size: 32 2106 32 1481 32 2747
Fragments arranged by size:
2747 2106 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2085 2374 2085
Size: 289 6141
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 2964 4000 2964
Size: 1036 5394
PspOMI G'GGCC_C
Cuts at: 2154 2154
Size: 6430
PstI C_TGCA'G
Cuts at: 830 3057 3127 3782 830
Size: 2227 70 655 3478
Fragments arranged by size:
3478 2227 655 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
SacII CC_GC'GG
Cuts at: 3380 3380
Size: 6430
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SfcI C'TryA_G
Cuts at: 826 1045 2347 3053 3123 3211 3448 3778 4913
Size: 219 1302 706 70 88 237 330 1135
Cuts at: 4913 5594 5785 826
Size: 681 191 1471
Fragments arranged by size:
1471 1302 1135 706 681 330 237 219
191 88 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6430
SmaI CCC'GGG
Cuts at: 1872 2153 3757 1872
Size: 281 1604 4545
Fragments arranged by size:
4545 1604 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2185 2576 4534 5435 5697 820
Size: 197 926 242 391 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 391 262 242 197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6430
StyI C'CwwG_G
Cuts at: 513 1065 2615 2811 3068 3462 3649 3869 513
Size: 552 1550 196 257 394 187 220 3074
Fragments arranged by size:
3074 1550 552 394 257 220 196 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2068 2389 3464 3526 4457 4474 4627
Size: 1255 488 321 1075 62 931 17 153
Cuts at: 4627 4812 5257 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1075 931 488 445 321 185
153 62 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2911 4676 6388
Size: 274 80 33 51 1443 934 1765 1712
Cuts at: 6388 96
Size: 138
Fragments arranged by size:
1765 1712 1443 934 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3505 3537 6390 6422 3505
Size: 32 2853 32 3513
Fragments arranged by size:
3513 2853 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2399 2540 1316
Size: 1083 141 5206
Fragments arranged by size:
5206 1083 141
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2858 3469 2858
Size: 611 5819
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6430
XmaI C'CCGG_G
Cuts at: 1870 2151 3755 1870
Size: 281 1604 4545
Fragments arranged by size:
4545 1604 281
XmnI GAAnn'nnTTC
Cuts at: 4559 4559
Size: 6430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI