Restriction map for
pFN21AE0059
(Circular) (Six-base) MAPSORT of: pFN21AE0059.seq Check: 9025 from: 1 to: 6130
pFN21AE0059 6130 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3473 1937
Size: 1536 4594
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2692 4193 5577 5845 1632
Size: 1060 1501 1384 268 1917
Fragments arranged by size:
1917 1501 1384 1060 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 2638 5029 6006 1203
Size: 1435 2391 977 1327
Fragments arranged by size:
2391 1435 1327 977
AgeI A'CCGG_T
Cuts at: 2750 4961 2750
Size: 2211 3919
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 2835 3087 3171 3339 1166
Size: 1669 252 84 168 3957
Fragments arranged by size:
3957 1669 252 168 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AlwNI CAG_nnn'CTG
Cuts at: 3873 5445 3873
Size: 1572 4558
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 2574 2618 2658 2826 2910 2994 3078 3162
Size: 1081 44 40 168 84 84 84 84
Cuts at: 3162 3246 3330 3414 3439 3665 3817 5926 1493
Size: 84 84 84 25 226 152 2109 1697
Fragments arranged by size:
2109 1697 1081 226 168 152 84 84
84 84 84 84 84 44 40 25
AseI AT'TA_AT
Cuts at: 160 1032 2493 4684 160
Size: 872 1461 2191 1606
Fragments arranged by size:
2191 1606 1461 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 3455 1870
Size: 73 1512 4545
Fragments arranged by size:
4545 1512 73
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3451 4906 618
Size: 325 217 627 1664 1455 1842
Fragments arranged by size:
1842 1664 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2196 2888 2972 3140 3449 1391
Size: 805 692 84 168 309 4072
Fragments arranged by size:
4072 805 692 309 168 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2508 928
Size: 971 609 4550
Fragments arranged by size:
4550 971 609
BbvCI CC'TCA_GC
Cuts at: 2010 2010
Size: 6130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2236 6125 2236
Size: 3889 2241
BlpI GC'TnA_GC
Cuts at: 3055 3547 3055
Size: 492 5638
Bme1580I G_kGCm'C
Cuts at: 1790 4191 5347 1790
Size: 2401 1156 2573
Fragments arranged by size:
2573 2401 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2405 3861 4819
Size: 416 53 83 399 24 988 1456 958
Cuts at: 4819 442
Size: 1753
Fragments arranged by size:
1753 1456 988 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2332 4790 1239
Size: 1093 2458 2579
Fragments arranged by size:
2579 2458 1093
Bpu10I CC'TnA_GC
Cuts at: 2010 2154 2010
Size: 144 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2145 2360 4255 5120 5418 2145
Size: 215 1895 865 298 2857
Fragments arranged by size:
2857 1895 865 298 215
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4319 4954 275
Size: 53 83 186 936 2404 382 635 1451
Fragments arranged by size:
2404 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2750 4563 4961 5235 5382 5793 2750
Size: 1813 398 274 147 411 3087
Fragments arranged by size:
3087 1813 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5905
Fragments arranged by size:
5905 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 1186
Size: 886 5244
BseYI C'CCAG_C
Cuts at: 5333 5829 5333
Size: 496 5634
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5557
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2888 2972 3140 4191 4276 5347 2888
Size: 84 168 1051 85 1071 3671
Fragments arranged by size:
3671 1071 1051 168 85 84
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2888 2972 3140 3449 4191 4276
Size: 399 406 692 84 168 309 742 85
Cuts at: 4276 5347 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 692 406 399 309 168
85 84
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2750 4774 4961 1700
Size: 110 940 2024 187 2869
Fragments arranged by size:
2869 2024 940 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 2236 3394 3460 3827 4212 4229 5681 5767
Size: 264 1158 66 367 385 17 1452 86
Cuts at: 5767 5779 6125 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1158 385 367 346 264 86
66 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 3646 4440 4460 3646
Size: 794 20 5316
Fragments arranged by size:
5316 794 20
ClaI AT'CG_AT
Cuts at: 1554 2246 1554
Size: 692 5438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3013 3048 529
Size: 35 2449 35 3611
Fragments arranged by size:
3611 2449 35 35
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095
Size: 54 1425 50 275 18 1747 889 1629
Cuts at: 6095 8
Size: 43
Fragments arranged by size:
1747 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4060 1353
Size: 2707 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2886 2970 3138 2886
Size: 84 168 5878
Fragments arranged by size:
5878 168 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2332 2692 4193 4790 5577 5845 1239
Size: 393 700 360 1501 597 787 268 1524
Fragments arranged by size:
1524 1501 787 700 597 393 360 268
EcoRI G'AATT_C
Cuts at: 2574 2826 2910 2994 3078 3162 3246 3330 3414
Size: 252 84 84 84 84 84 84 84
Cuts at: 3414 3439 3817 2574
Size: 25 378 4887
Fragments arranged by size:
4887 378 252 84 84 84 84 84
84 84 25
EcoRV GAT'ATC
Cuts at: 2402 2402
Size: 6130
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 5277 1678
Size: 3599 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 4771 4803 4845
Size: 32 135 32 656 32 2511 32 42
Cuts at: 4845 4877 1373
Size: 32 2626
Fragments arranged by size:
2626 2511 656 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3152 3474 3720 669
Size: 988 281 1214 322 246 3079
Fragments arranged by size:
3079 1214 988 322 281 246
HindIII A'AGCT_T
Cuts at: 748 2975 748
Size: 2227 3903
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 5244 5428 1305
Size: 786 3153 184 2007
Fragments arranged by size:
3153 2007 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4308
Fragments arranged by size:
4308 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2583 2835 2919 3003 3087 3171 3255
Size: 648 1417 252 84 84 84 84 84
Cuts at: 3255 3271 3339 4088 4447 4606 518
Size: 16 68 749 359 159 2042
Fragments arranged by size:
2042 1417 749 648 359 252 159 84
84 84 84 84 68 16
MspA1I CmG'CkG
Cuts at: 1606 3546 4223 5371 5616 1606
Size: 1940 677 1148 245 2120
Fragments arranged by size:
2120 1940 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
NdeI CA'TA_TG
Cuts at: 387 3268 387
Size: 2881 3249
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2449 2642 2906 3488 5033 1132
Size: 97 1220 193 264 582 1545 2229
Fragments arranged by size:
2229 1545 1220 582 264 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 2638 5029 2638
Size: 2391 3739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6130
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 3482 830
Size: 2652 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
SacI G_AGCT'C
Cuts at: 2888 2972 3140 2888
Size: 84 168 5878
Fragments arranged by size:
5878 168 84
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 2345 4378 6090 2345
Size: 2033 1712 2385
Fragments arranged by size:
2385 2033 1712
SfcI C'TryA_G
Cuts at: 826 1045 3478 4613 5294 5485 826
Size: 219 2433 1135 681 191 1471
Fragments arranged by size:
2433 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2160 2339 4234 5135 5397 820
Size: 197 926 217 179 1895 901 262 1553
Fragments arranged by size:
1895 1553 926 901 262 217 197 179
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 2906 3488 1132
Size: 1774 582 3774
Fragments arranged by size:
3774 1774 582
SspI AAT'ATT
Cuts at: 5 52 2430 4054 5
Size: 47 2378 1624 2081
Fragments arranged by size:
2378 2081 1624 47
StuI AGG'CCT
Cuts at: 1517 2226 2537 2705 2789 3209 3293 1517
Size: 709 311 168 84 420 84 4354
Fragments arranged by size:
4354 709 420 311 168 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 3569 513
Size: 552 939 1565 3074
Fragments arranged by size:
3074 1565 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4157 4174 4327 4512 4957 325
Size: 1255 2577 17 153 185 445 1498
Fragments arranged by size:
2577 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 4376 6088
Size: 274 80 33 51 1443 366 2033 1712
Cuts at: 6088 96
Size: 138
Fragments arranged by size:
2033 1712 1443 366 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2234 2266 3216 3248 6090 6122 2234
Size: 32 950 32 2842 32 2242
Fragments arranged by size:
2842 2242 950 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI