Restriction map for
pFN21AE0058
(Circular) (Six-base) MAPSORT of: pFN21AE0058.seq Check: 844 from: 1 to: 6982
pFN21AE0058 6982 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2623 2623
Size: 6982
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4792 278
Size: 53 83 186 936 3256 2468
Fragments arranged by size:
3256 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4325 1937
Size: 2388 4594
Acc65I G'GTAC_C
Cuts at: 4303 4303
Size: 6982
AclI AA'CG_TT
Cuts at: 1563 5109 5482 1563
Size: 3546 373 3063
Fragments arranged by size:
3546 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2802 2817 3407 5045 6429 6697 1632
Size: 1170 15 590 1638 1384 268 1917
Fragments arranged by size:
1917 1638 1384 1170 590 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6785
AflIII A'CryG_T
Cuts at: 1203 3534 5881 6858 1203
Size: 2331 2347 977 1327
Fragments arranged by size:
2347 2331 1327 977
AgeI A'CCGG_T
Cuts at: 5813 5813
Size: 6982
AhdI GACnn_n'nnGTC
Cuts at: 5711 5711
Size: 6982
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6982
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6982
AlwNI CAG_nnn'CTG
Cuts at: 2354 2615 4725 6297 2354
Size: 261 2110 1572 3039
Fragments arranged by size:
3039 2110 1572 261
ApaI G_GGCC'C
Cuts at: 4115 4115
Size: 6982
ApaLI G'TGCA_C
Cuts at: 5039 6195 5039
Size: 1156 5826
ApoI r'AATT_y
Cuts at: 1493 2344 2838 3173 3229 3827 4291 4517 4669
Size: 851 494 335 56 598 464 226 152
Cuts at: 4669 6778 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 851 598 494 464 335 226
152 56
AseI AT'TA_AT
Cuts at: 160 1032 5536 160
Size: 872 4504 1606
Fragments arranged by size:
4504 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6982
AvaI C'yCGr_G
Cuts at: 1870 1943 3585 4307 1870
Size: 73 1642 722 4545
Fragments arranged by size:
4545 1642 722 73
BamHI G'GATC_C
Cuts at: 4312 4679 4312
Size: 367 6615
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2803 3978 4303 5758 618
Size: 325 217 627 1016 1175 325 1455 1842
Fragments arranged by size:
1842 1455 1175 1016 627 325 325 217
BanII G_rGCy'C
Cuts at: 1391 2544 3199 4115 4301 1391
Size: 1153 655 916 186 4072
Fragments arranged by size:
4072 1153 916 655 186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2522 928
Size: 971 623 5388
Fragments arranged by size:
5388 971 623
BbvCI CC'TCA_GC
Cuts at: 3864 3864
Size: 6982
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3601 3635 5173 5207 1903
Size: 34 1664 34 1538 34 3678
Fragments arranged by size:
3678 1664 1538 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2561 4874 6084 2561
Size: 2313 1210 3459
Fragments arranged by size:
3459 2313 1210
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 6523
BfrBI ATG'CAT
Cuts at: 3618 3618
Size: 6982
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5593 136
Size: 107 122 71 631 605 3921 1525
Fragments arranged by size:
3921 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3029 6977 3029
Size: 3948 3034
BlpI GC'TnA_GC
Cuts at: 4399 4399
Size: 6982
Bme1580I G_kGCm'C
Cuts at: 1790 2806 4115 5043 6199 1790
Size: 1016 1309 928 1156 2573
Fragments arranged by size:
2573 1309 1156 1016 928
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6982
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 2520 4713
Size: 416 53 83 399 24 639 464 2193
Cuts at: 4713 5671 442
Size: 958 1753
Fragments arranged by size:
2193 1753 958 639 464 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2757 1056
Size: 1701 5281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2409 2424 2716 2980 3432 5642 1239
Size: 1170 15 292 264 452 2210 2579
Fragments arranged by size:
2579 2210 1170 452 292 264 15
Bpu10I CC'TnA_GC
Cuts at: 3124 3864 3124
Size: 740 6242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3768 5107 5972 6270 3768
Size: 1339 865 298 4480
Fragments arranged by size:
4480 1339 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2109 3479 5645 882
Size: 617 610 1370 2166 2219
Fragments arranged by size:
2219 2166 1370 617 610
BsaAI yAC'GTr
Cuts at: 493 1204 4773 493
Size: 711 3569 2702
Fragments arranged by size:
3569 2702 711
BsaBI GATnn'nnATC
Cuts at: 3244 6976 3244
Size: 3732 3250
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 4789 5171 5806
Size: 53 83 186 936 515 2741 382 635
Cuts at: 5806 275
Size: 1451
Fragments arranged by size:
2741 1451 936 635 515 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2377 5415 5813 6087 6234 6645 2377
Size: 3038 398 274 147 411 2714
Fragments arranged by size:
3038 2714 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2089 2119 1178
Size: 30 165 30 686 30 6041
Fragments arranged by size:
6041 686 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2673 2754 3305 3518 3769 1186
Size: 1487 81 551 213 251 4399
Fragments arranged by size:
4399 1487 551 251 213 81
BseYI C'CCAG_C
Cuts at: 3631 4157 6185 6681 3631
Size: 526 2028 496 3932
Fragments arranged by size:
3932 2028 526 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2601 3369 1163
Size: 573 865 768 4776
Fragments arranged by size:
4776 865 768 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2308 4432 5193 6221 1540
Size: 275 186 307 2124 761 1028 2301
Fragments arranged by size:
2301 2124 1028 761 307 275 186
BsiHKAI G_wGCw'C
Cuts at: 3306 5043 5128 6199 3306
Size: 1737 85 1071 4089
Fragments arranged by size:
4089 1737 1071 85
BsmI GAATG_Cn'
Cuts at: 2587 3060 4493 4586 2587
Size: 473 1433 93 4983
Fragments arranged by size:
4983 1433 473 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2643 2643
Size: 6982
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2544 2806 3199 3306 4115 4301 5043
Size: 399 754 262 393 107 809 186 742
Cuts at: 5043 5128 6199 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 809 754 742 399 393 262
186 107 85
BspEI T'CCGG_A
Cuts at: 2377 6645 2377
Size: 4268 2714
BspHI T'CATG_A
Cuts at: 3936 4871 6601 3936
Size: 935 1730 4317
Fragments arranged by size:
4317 1730 935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2623 2835 4337 844
Size: 1779 212 1502 3489
Fragments arranged by size:
3489 1779 1502 212
BsrBI CCG'CTC
Cuts at: 1394 1823 4869 1394
Size: 429 3046 3507
Fragments arranged by size:
3507 3046 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3465 5477 5651 65
Size: 1183 2217 2012 174 1396
Fragments arranged by size:
2217 2012 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3656 5626 5813 1700
Size: 110 1846 1970 187 2869
Fragments arranged by size:
2869 1970 1846 187 110
BsrGI T'GTAC_A
Cuts at: 96 3048 96
Size: 2952 4030
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6982
BssSI C'ACGA_G
Cuts at: 5042 6054 5042
Size: 1012 5970
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6982
BstBI TT'CG_AA
Cuts at: 5786 5786
Size: 6982
BstEII G'GTnAC_C
Cuts at: 1184 3418 1184
Size: 2234 4748
BstXI CCAn_nnnn'nTGG
Cuts at: 2897 3456 2897
Size: 559 6423
BstYI r'GATC_y
Cuts at: 1972 3029 3245 4312 4679 5064 5081 6533 6619
Size: 1057 216 1067 367 385 17 1452 86
Cuts at: 6619 6631 6977 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1067 1057 385 367 346 216
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2896 513
Size: 552 1831 4599
Fragments arranged by size:
4599 1831 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3258 3604 487
Size: 683 56 2032 346 3865
Fragments arranged by size:
3865 2032 683 346 56
BtsI GCAGTG_nn'
Cuts at: 2048 2388 4040 4110 4498 5292 5312 2048
Size: 340 1652 70 388 794 20 3718
Fragments arranged by size:
3718 1652 794 388 340 70 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6982
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2679 2714 529
Size: 35 2115 35 4797
Fragments arranged by size:
4797 2115 35 35
DraI TTT'AAA
Cuts at: 4286 4286
Size: 6982
DraIII CAC_nnn'GTG
Cuts at: 3373 3373
Size: 6982
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5989 809
Size: 507 4673 1802
Fragments arranged by size:
4673 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2066 3019 3515
Size: 54 1425 50 275 18 236 953 496
Cuts at: 3515 3723 4429 5318 6947 8
Size: 208 706 889 1629 43
Fragments arranged by size:
1629 1425 953 889 706 496 275 236
208 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4429 1537
Size: 275 2617 4090
Fragments arranged by size:
4090 2617 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4912 1353
Size: 3559 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2676 2802 5569 5943 6089 387
Size: 1064 138 1087 126 2767 374 146 1280
Fragments arranged by size:
2767 1280 1087 1064 374 146 138 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2409 2424 2716 2802 2817 2980 3407
Size: 393 777 15 292 86 15 163 427
Cuts at: 3407 3432 5045 5642 6429 6697 1239
Size: 25 1613 597 787 268 1524
Fragments arranged by size:
1613 1524 787 777 597 427 393 292
268 163 86 25 15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3485 3485
Size: 6982
EcoO109I rG'GnC_Cy
Cuts at: 2246 3335 3443 4112 2246
Size: 1089 108 669 5116
Fragments arranged by size:
5116 1089 669 108
EcoRI G'AATT_C
Cuts at: 2344 2838 4291 4669 2344
Size: 494 1453 378 4657
Fragments arranged by size:
4657 1453 494 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2979 2979
Size: 6982
FspI TGC'GCA
Cuts at: 5488 5488
Size: 6982
HaeII r_GCGC'y
Cuts at: 1678 6129 1678
Size: 4451 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 2165 2197 2250
Size: 32 135 32 503 32 58 32 53
Cuts at: 2250 2273 2282 2305 2978 3010 3936 3968 5623
Size: 23 9 23 673 32 926 32 1655
Cuts at: 5623 5655 5697 5729 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1655 926 673 503 135 58 53
42 32 32 32 32 32 32 32
32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 4326 4572 669
Size: 988 281 2388 246 3079
Fragments arranged by size:
3079 2388 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2961 748
Size: 2213 4769
HpaI GTT'AAC
Cuts at: 4572 4572
Size: 6982
KpnI G_GTAC'C
Cuts at: 4307 4307
Size: 6982
MfeI C'AATT_G
Cuts at: 4581 4581
Size: 6982
MluI A'CGCG_T
Cuts at: 3534 6858 3534
Size: 3324 3658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 2288 3387 3794 6096 6280 1305
Size: 918 65 1099 407 2302 184 2007
Fragments arranged by size:
2302 2007 1099 918 407 184 65
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2068 3021 3517 3725 10
Size: 54 1425 343 236 953 496 208 3267
Fragments arranged by size:
3267 1425 953 496 343 236 208 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3767 4940 5299 5458 518
Size: 648 2601 1173 359 159 2042
Fragments arranged by size:
2601 2042 1173 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3631 4398 5075 6223 6468 1606
Size: 2025 767 677 1148 245 2120
Fragments arranged by size:
2120 2025 1148 767 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2896 513
Size: 552 1831 4599
Fragments arranged by size:
4599 1831 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6982
NheI G'CTAG_C
Cuts at: 1052 2753 1052
Size: 1701 5281
NotI GC'GGCC_GC
Cuts at: 4429 4429
Size: 6982
NsiI A_TGCA'T
Cuts at: 3620 3620
Size: 6982
NspI r_CATG'y
Cuts at: 1132 1229 2060 3772 4340 5885 1132
Size: 97 831 1712 568 1545 2229
Fragments arranged by size:
2229 1712 1545 831 568 97
PasI CC'CwG_GG
Cuts at: 1348 2795 1348
Size: 1447 5535
PciI A'CATG_T
Cuts at: 5881 5881
Size: 6982
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3373 1108
Size: 2265 4717
PmeI GTTT'AAAC
Cuts at: 4286 4286
Size: 6982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5051 5083 6564 6596 5051
Size: 32 1481 32 5437
Fragments arranged by size:
5437 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2246 3443 2246
Size: 1197 5785
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6982
PsiI TTA'TAA
Cuts at: 4552 4552
Size: 6982
PspOMI G'GGCC_C
Cuts at: 4111 4111
Size: 6982
PstI C_TGCA'G
Cuts at: 830 2145 4334 830
Size: 1315 2189 3478
Fragments arranged by size:
3478 2189 1315
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6982
PvuII CAG'CTG
Cuts at: 3631 3631
Size: 6982
SalI G'TCGA_C
Cuts at: 1936 4324 1936
Size: 2388 4594
SbfI CC_TGCA'GG
Cuts at: 4334 4334
Size: 6982
ScaI AGT'ACT
Cuts at: 3220 5230 6942 3220
Size: 2010 1712 3260
Fragments arranged by size:
3260 2010 1712
SexAI A'CCwGG_T
Cuts at: 3414 3480 3414
Size: 66 6916
SfcI C'TryA_G
Cuts at: 826 1045 2128 2141 2663 3214 4330 5465 6146
Size: 219 1083 13 522 551 1116 1135 681
Cuts at: 6146 6337 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1116 1083 681 551 522 219
191 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6982
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6982
SmaI CCC'GGG
Cuts at: 1872 4309 1872
Size: 2437 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3783 5086 5987 6249 820
Size: 197 926 1840 1303 901 262 1553
Fragments arranged by size:
1840 1553 1303 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4773 493
Size: 4280 2702
SpeI A'CTAG_T
Cuts at: 152 2296 3667 152
Size: 2144 1371 3467
Fragments arranged by size:
3467 2144 1371
SphI G_CATG'C
Cuts at: 1132 2060 4340 1132
Size: 928 2280 3774
Fragments arranged by size:
3774 2280 928
SspI AAT'ATT
Cuts at: 5 52 4906 5
Size: 47 4854 2081
Fragments arranged by size:
4854 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6982
StyI C'CwwG_G
Cuts at: 513 1065 2697 2896 3439 4421 513
Size: 552 1632 199 543 982 3074
Fragments arranged by size:
3074 1632 982 552 543 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2091 2271 3339 3452 5009 5026 5179
Size: 1255 511 180 1068 113 1557 17 153
Cuts at: 5179 5364 5809 325
Size: 185 445 1498
Fragments arranged by size:
1557 1498 1255 1068 511 445 185 180
153 113 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3048 3218 3267
Size: 274 80 33 51 1443 1071 170 49
Cuts at: 3267 3850 3991 5228 6940 96
Size: 583 141 1237 1712 138
Fragments arranged by size:
1712 1443 1237 1071 583 274 170 141
138 80 51 49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2089 2121 2857 2889 3435 3467 4032 4064 4065
Size: 32 736 32 546 32 565 32 1
Cuts at: 4065 4097 6942 6974 2089
Size: 32 2845 32 2097
Fragments arranged by size:
2845 2097 736 565 546 32 32 32
32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6982
XbaI T'CTAG_A
Cuts at: 4318 4318
Size: 6982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3370 3370
Size: 6982
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6982
XmaI C'CCGG_G
Cuts at: 1870 4307 1870
Size: 2437 4545
XmnI GAAnn'nnTTC
Cuts at: 2963 3921 5111 2963
Size: 958 1190 4834
Fragments arranged by size:
4834 1190 958
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4790 276
Size: 53 83 186 936 3256 2468
Fragments arranged by size:
3256 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI