Restriction map for
pFN21AE0052
(Circular) (Six-base) MAPSORT of: pFN21AE0052.seq Check: 505 from: 1 to: 7966
pFN21AE0052 7966 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5776 278
Size: 53 83 186 936 4240 2468
Fragments arranged by size:
4240 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4315 4740 5309 1937
Size: 2378 425 569 4594
Fragments arranged by size:
4594 2378 569 425
Acc65I G'GTAC_C
Cuts at: 5287 5287
Size: 7966
AclI AA'CG_TT
Cuts at: 1563 4491 6093 6466 1563
Size: 2928 1602 373 3063
Fragments arranged by size:
3063 2928 1602 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2021 2927 2982 3029 3528 5183 6029 7413
Size: 389 906 55 47 499 1655 846 1384
Cuts at: 7413 7681 1632
Size: 268 1917
Fragments arranged by size:
1917 1655 1384 906 846 499 389 268
55 47
AflII C'TTAA_G
Cuts at: 820 1017 3926 820
Size: 197 2909 4860
Fragments arranged by size:
4860 2909 197
AflIII A'CryG_T
Cuts at: 1203 2222 6865 7842 1203
Size: 1019 4643 977 1327
Fragments arranged by size:
4643 1327 1019 977
AgeI A'CCGG_T
Cuts at: 6797 6797
Size: 7966
AhdI GACnn_n'nnGTC
Cuts at: 6695 6695
Size: 7966
AleI CACnn'nnGTG
Cuts at: 1166 2758 4748 1166
Size: 1592 1990 4384
Fragments arranged by size:
4384 1990 1592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7966
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 7934
AlwNI CAG_nnn'CTG
Cuts at: 5709 7281 5709
Size: 1572 6394
ApaLI G'TGCA_C
Cuts at: 6023 7179 6023
Size: 1156 6810
ApoI r'AATT_y
Cuts at: 1493 2432 2660 3190 3743 3817 4028 4341 5157
Size: 939 228 530 553 74 211 313 816
Cuts at: 5157 5203 5275 5501 5653 7762 1493
Size: 46 72 226 152 2109 1697
Fragments arranged by size:
2109 1697 939 816 553 530 313 228
226 211 152 74 72 46
AseI AT'TA_AT
Cuts at: 160 1032 2888 3312 4260 4575 6520 160
Size: 872 1856 424 948 315 1945 1606
Fragments arranged by size:
1945 1856 1606 948 872 424 315
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7966
AvaI C'yCGr_G
Cuts at: 1870 1943 2177 5291 1870
Size: 73 234 3114 4545
Fragments arranged by size:
4545 3114 234 73
BamHI G'GATC_C
Cuts at: 5296 5663 5296
Size: 367 7599
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2181 5287 6742 618
Size: 325 217 627 394 3106 1455 1842
Fragments arranged by size:
3106 1842 1455 627 394 325 217
BanII G_rGCy'C
Cuts at: 1391 2145 4919 5285 1391
Size: 754 2774 366 4072
Fragments arranged by size:
4072 2774 754 366
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2585 3049 3452 3653 4440 5181 928
Size: 971 686 464 403 201 787 741 3713
Fragments arranged by size:
3713 971 787 741 686 464 403 201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6157 6191 1903
Size: 34 4220 34 3678
Fragments arranged by size:
4220 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5858 7068 5858
Size: 1210 6756
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7966
BfrBI ATG'CAT
Cuts at: 2885 3206 4361 4646 2885
Size: 321 1155 285 6205
Fragments arranged by size:
6205 1155 321 285
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6577 136
Size: 107 122 71 631 605 4905 1525
Fragments arranged by size:
4905 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3910 4562 7961 3910
Size: 652 3399 3915
Fragments arranged by size:
3915 3399 652
BlpI GC'TnA_GC
Cuts at: 5383 5383
Size: 7966
Bme1580I G_kGCm'C
Cuts at: 1790 2934 6027 7183 1790
Size: 1144 3093 1156 2573
Fragments arranged by size:
3093 2573 1156 1144
BmgBI CAC'GTC
Cuts at: 1318 2101 1318
Size: 783 7183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3876 5697 6655
Size: 416 53 83 399 24 2459 1821 958
Cuts at: 6655 442
Size: 1753
Fragments arranged by size:
2459 1821 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7966
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2613 2613
Size: 7966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2398 2840 4872 4941 5023 6626 1239
Size: 1159 442 2032 69 82 1603 2579
Fragments arranged by size:
2579 2032 1603 1159 442 82 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3994 6091 6956 7254 3994
Size: 2097 865 298 4706
Fragments arranged by size:
4706 2097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2416 6629 882
Size: 617 917 4213 2219
Fragments arranged by size:
4213 2219 917 617
BsaAI yAC'GTr
Cuts at: 493 1204 5757 493
Size: 711 4553 2702
Fragments arranged by size:
4553 2702 711
BsaBI GATnn'nnATC
Cuts at: 3345 3372 7960 3345
Size: 27 4588 3351
Fragments arranged by size:
4588 3351 27
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 4015 5773 6155
Size: 53 83 186 936 600 1882 1758 382
Cuts at: 6155 6790 275
Size: 635 1451
Fragments arranged by size:
1882 1758 1451 936 635 600 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4800 6399 6797 7071 7218 7629 4800
Size: 1599 398 274 147 411 5137
Fragments arranged by size:
5137 1599 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2034 2064 1178
Size: 30 165 30 631 30 7080
Fragments arranged by size:
7080 631 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2120 2153 4697 4802 4861 1186
Size: 901 33 33 2544 105 59 4291
Fragments arranged by size:
4291 2544 901 105 59 33 33
BseYI C'CCAG_C
Cuts at: 4937 7169 7665 4937
Size: 2232 496 5238
Fragments arranged by size:
5238 2232 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7393
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5416 6177 7205 1540
Size: 275 186 3415 761 1028 2301
Fragments arranged by size:
3415 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2389 3834 4919 6027 6112 7183 2145
Size: 244 1445 1085 1108 85 1071 2928
Fragments arranged by size:
2928 1445 1108 1085 1071 244 85
BsmI GAATG_Cn'
Cuts at: 2887 3544 4363 4810 5477 5570 2887
Size: 657 819 447 667 93 5283
Fragments arranged by size:
5283 819 667 657 447 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2389 2934 3834 4919 5285 6027
Size: 399 355 244 545 900 1085 366 742
Cuts at: 6027 6112 7183 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1085 1071 900 742 545 399 366
355 244 85
BspEI T'CCGG_A
Cuts at: 4800 7629 4800
Size: 2829 5137
BspHI T'CATG_A
Cuts at: 5855 7585 5855
Size: 1730 6236
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2034 5321 844
Size: 1190 3287 3489
Fragments arranged by size:
3489 3287 1190
BsrBI CCG'CTC
Cuts at: 1394 1823 5853 1394
Size: 429 4030 3507
Fragments arranged by size:
4030 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4510 6461 6635 65
Size: 1183 3262 1951 174 1396
Fragments arranged by size:
3262 1951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6610 6797 1700
Size: 110 4800 187 2869
Fragments arranged by size:
4800 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7966
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7966
BssSI C'ACGA_G
Cuts at: 6026 7038 6026
Size: 1012 6954
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7966
BstBI TT'CG_AA
Cuts at: 6770 6770
Size: 7966
BstEII G'GTnAC_C
Cuts at: 1184 3278 1184
Size: 2094 5872
BstXI CCAn_nnnn'nTGG
Cuts at: 2065 2065
Size: 7966
BstYI r'GATC_y
Cuts at: 1972 2062 3910 4562 5296 5663 6048 6065 7517
Size: 90 1848 652 734 367 385 17 1452
Cuts at: 7517 7603 7615 7961 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1848 1452 734 652 385 367 346
90 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2759 3737 4907 513
Size: 552 939 755 978 1170 3572
Fragments arranged by size:
3572 1170 978 939 755 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2824 3076 3213 487
Size: 683 56 1598 252 137 5240
Fragments arranged by size:
5240 1598 683 252 137 56
BtsI GCAGTG_nn'
Cuts at: 2155 5482 6276 6296 2155
Size: 3327 794 20 3825
Fragments arranged by size:
3825 3327 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7966
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7931
DraI TTT'AAA
Cuts at: 2692 5270 2692
Size: 2578 5388
DraIII CAC_nnn'GTG
Cuts at: 3678 3678
Size: 7966
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3928 6973 809
Size: 507 2612 3045 1802
Fragments arranged by size:
3045 2612 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 5413 6302
Size: 54 1425 50 275 18 177 3406 889
Cuts at: 6302 7931 8
Size: 1629 43
Fragments arranged by size:
3406 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5413 1537
Size: 275 3601 4090
Fragments arranged by size:
4090 3601 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2559 2733 2856 4110 4907 5020 5896
Size: 756 450 174 123 1254 797 113 876
Cuts at: 5896 1353
Size: 3423
Fragments arranged by size:
3423 1254 876 797 756 450 174 123
113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3602 6553 6927 7073 387
Size: 1064 138 2013 2951 374 146 1280
Fragments arranged by size:
2951 2013 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2143 4917 2143
Size: 2774 5192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2021 2398 2840 2927 2982 3029 3528
Size: 393 389 377 442 87 55 47 499
Cuts at: 3528 4872 4941 5023 5183 6029 6626 7413 7681
Size: 1344 69 82 160 846 597 787 268
Cuts at: 7681 1239
Size: 1524
Fragments arranged by size:
1524 1344 846 787 597 499 442 393
389 377 268 160 87 82 69 55
47
EcoO109I rG'GnC_Cy
Cuts at: 2042 4934 2042
Size: 2892 5074
EcoRI G'AATT_C
Cuts at: 2660 3190 5275 5653 2660
Size: 530 2085 378 4973
Fragments arranged by size:
4973 2085 530 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4495 4495
Size: 7966
FspI TGC'GCA
Cuts at: 6472 6472
Size: 7966
HaeII r_GCGC'y
Cuts at: 1678 7113 1678
Size: 5435 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 2102 2134 4886
Size: 32 135 32 462 32 36 32 2752
Cuts at: 4886 4918 6607 6639 6681 6713 1373
Size: 32 1689 32 42 32 2626
Fragments arranged by size:
2752 2626 1689 462 135 42 36 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3578 5310 5556 669
Size: 988 281 1640 1732 246 3079
Fragments arranged by size:
3079 1732 1640 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3848 748
Size: 3100 4866
HpaI GTT'AAC
Cuts at: 5556 5556
Size: 7966
KpnI G_GTAC'C
Cuts at: 5291 5291
Size: 7966
MfeI C'AATT_G
Cuts at: 5565 5565
Size: 7966
MluI A'CGCG_T
Cuts at: 7842 7842
Size: 7966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2804 3468 4085 7080 7264 1305
Size: 1499 664 617 2995 184 2007
Fragments arranged by size:
2995 2007 1499 664 617 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 5967
Fragments arranged by size:
5967 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2382 2758 2937 3138 4748 5924 6283
Size: 648 1216 376 179 201 1610 1176 359
Cuts at: 6283 6442 518
Size: 159 2042
Fragments arranged by size:
2042 1610 1216 1176 648 376 359 201
179 159
MspA1I CmG'CkG
Cuts at: 1606 2860 3337 3635 4764 5382 6059 7207 7452
Size: 1254 477 298 1129 618 677 1148 245
Cuts at: 7452 1606
Size: 2120
Fragments arranged by size:
2120 1254 1148 1129 677 618 477 298
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3737 513
Size: 552 939 1733 4742
Fragments arranged by size:
4742 1733 939 552
NdeI CA'TA_TG
Cuts at: 387 2938 2979 3040 387
Size: 2551 41 61 5313
Fragments arranged by size:
5313 2551 61 41
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7966
NotI GC'GGCC_GC
Cuts at: 5413 5413
Size: 7966
NruI TCG'CGA
Cuts at: 5107 5107
Size: 7966
NsiI A_TGCA'T
Cuts at: 2887 3208 4363 4648 2887
Size: 321 1155 285 6205
Fragments arranged by size:
6205 1155 321 285
NspI r_CATG'y
Cuts at: 1132 1229 2226 4646 4739 5324 6869 1132
Size: 97 997 2420 93 585 1545 2229
Fragments arranged by size:
2420 2229 1545 997 585 97 93
PasI CC'CwG_GG
Cuts at: 1348 2058 1348
Size: 710 7256
PciI A'CATG_T
Cuts at: 2222 6865 2222
Size: 4643 3323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7966
PmeI GTTT'AAAC
Cuts at: 5270 5270
Size: 7966
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2140 6035 6067 7548 7580 2108
Size: 32 3895 32 1481 32 2494
Fragments arranged by size:
3895 2494 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2042 4934 2042
Size: 2892 5074
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7966
PsiI TTA'TAA
Cuts at: 5536 5536
Size: 7966
PstI C_TGCA'G
Cuts at: 830 2150 4174 5318 830
Size: 1320 2024 1144 3478
Fragments arranged by size:
3478 2024 1320 1144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7966
PvuII CAG'CTG
Cuts at: 2860 3337 3635 2860
Size: 477 298 7191
Fragments arranged by size:
7191 477 298
SacI G_AGCT'C
Cuts at: 2145 4919 2145
Size: 2774 5192
SalI G'TCGA_C
Cuts at: 1936 5308 1936
Size: 3372 4594
SapI GCTCTTCn'nnn_
Cuts at: 4907 4907
Size: 7966
SbfI CC_TGCA'GG
Cuts at: 5318 5318
Size: 7966
ScaI AGT'ACT
Cuts at: 6214 7926 6214
Size: 1712 6254
SexAI A'CCwGG_T
Cuts at: 3892 3892
Size: 7966
SfcI C'TryA_G
Cuts at: 826 1045 2146 2788 3597 3621 3792 4170 4965
Size: 219 1101 642 809 24 171 378 795
Cuts at: 4965 5314 6449 7130 7321 826
Size: 349 1135 681 191 1471
Fragments arranged by size:
1471 1135 1101 809 795 681 642 378
349 219 191 171 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7966
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7966
SmaI CCC'GGG
Cuts at: 1872 2179 5293 1872
Size: 307 3114 4545
Fragments arranged by size:
4545 3114 307
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3926 4009 6070 6971 7233 820
Size: 197 926 1983 83 2061 901 262 1553
Fragments arranged by size:
2061 1983 1553 926 901 262 197 83
SnaBI TAC'GTA
Cuts at: 493 5757 493
Size: 5264 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7966
SphI G_CATG'C
Cuts at: 1132 5324 1132
Size: 4192 3774
SspI AAT'ATT
Cuts at: 5 52 4628 5890 5
Size: 47 4576 1262 2081
Fragments arranged by size:
4576 2081 1262 47
StuI AGG'CCT
Cuts at: 1517 5012 1517
Size: 3495 4471
StyI C'CwwG_G
Cuts at: 513 1065 2004 3128 3737 3859 5405 513
Size: 552 939 1124 609 122 1546 3074
Fragments arranged by size:
3074 1546 1124 939 609 552 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2071 3799 5993 6010 6163 6348 6793
Size: 1255 491 1728 2194 17 153 185 445
Cuts at: 6793 325
Size: 1498
Fragments arranged by size:
2194 1728 1498 1255 491 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6212 7924 96
Size: 274 80 33 51 1443 4235 1712 138
Fragments arranged by size:
4235 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3283 3315 7926 7958 3283
Size: 32 4611 32 3291
Fragments arranged by size:
4611 3291 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2968 1316
Size: 1652 6314
XbaI T'CTAG_A
Cuts at: 4417 5302 4417
Size: 885 7081
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 2519 4579 2065
Size: 454 2060 5452
Fragments arranged by size:
5452 2060 454
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7966
XmaI C'CCGG_G
Cuts at: 1870 2177 5291 1870
Size: 307 3114 4545
Fragments arranged by size:
4545 3114 307
XmnI GAAnn'nnTTC
Cuts at: 2664 4850 6095 2664
Size: 2186 1245 4535
Fragments arranged by size:
4535 2186 1245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5774 276
Size: 53 83 186 936 4240 2468
Fragments arranged by size:
4240 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI