Restriction map for
pFN21AE0051
(Circular) (Six-base) MAPSORT of: pFN21AE0051.seq Check: 1466 from: 1 to: 7708
pFN21AE0051 7708 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2963 2963
Size: 7708
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5518 278
Size: 53 83 186 936 3982 2468
Fragments arranged by size:
3982 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2391 5051 1937
Size: 454 2660 4594
Fragments arranged by size:
4594 2660 454
Acc65I G'GTAC_C
Cuts at: 2969 5029 2969
Size: 2060 5648
AclI AA'CG_TT
Cuts at: 1563 5835 6208 1563
Size: 4272 373 3063
Fragments arranged by size:
4272 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 4157 5771 7155 7423 1632
Size: 749 1776 1614 1384 268 1917
Fragments arranged by size:
1917 1776 1614 1384 749 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7511
AflIII A'CryG_T
Cuts at: 1203 6607 7584 1203
Size: 5404 977 1327
Fragments arranged by size:
5404 1327 977
AgeI A'CCGG_T
Cuts at: 2681 6539 2681
Size: 3858 3850
AhdI GACnn_n'nnGTC
Cuts at: 2407 3478 6437 2407
Size: 1071 2959 3678
Fragments arranged by size:
3678 2959 1071
AleI CACnn'nnGTG
Cuts at: 1166 2684 3347 3377 3605 4178 1166
Size: 1518 663 30 228 573 4696
Fragments arranged by size:
4696 1518 663 573 228 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2429 1184
Size: 1245 6463
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4736 4768 4736
Size: 32 7676
AlwNI CAG_nnn'CTG
Cuts at: 2761 4497 4768 5451 7023 2761
Size: 1736 271 683 1572 3446
Fragments arranged by size:
3446 1736 1572 683 271
ApaI G_GGCC'C
Cuts at: 4888 4888
Size: 7708
ApaLI G'TGCA_C
Cuts at: 4630 5765 6921 4630
Size: 1135 1156 5417
Fragments arranged by size:
5417 1156 1135
ApoI r'AATT_y
Cuts at: 1493 3449 5017 5243 5395 7504 1493
Size: 1956 1568 226 152 2109 1697
Fragments arranged by size:
2109 1956 1697 1568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6262 160
Size: 872 5230 1606
Fragments arranged by size:
5230 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7708
AvaI C'yCGr_G
Cuts at: 1870 1943 2430 2595 2632 3468 3516 3603 3702
Size: 73 487 165 37 836 48 87 99
Cuts at: 3702 4685 5033 1870
Size: 983 348 4545
Fragments arranged by size:
4545 983 836 487 348 165 99 87
73 48 37
BamHI G'GATC_C
Cuts at: 3858 4984 5038 5405 3858
Size: 1126 54 367 6161
Fragments arranged by size:
6161 1126 367 54
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2636 2690 2969 3065 3551
Size: 325 217 627 849 54 279 96 486
Cuts at: 3551 3596 3607 5029 6484 618
Size: 45 11 1422 1455 1842
Fragments arranged by size:
1842 1455 1422 849 627 486 325 279
217 96 54 45 11
BanII G_rGCy'C
Cuts at: 1391 2127 2583 2864 3833 4047 4410 4475 4485
Size: 736 456 281 969 214 363 65 10
Cuts at: 4485 4692 4811 4888 4944 5027 1391
Size: 207 119 77 56 83 4072
Fragments arranged by size:
4072 969 736 456 363 281 214 207
119 83 77 65 56 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 2184 2927 4666 928
Size: 971 251 34 743 1739 3970
Fragments arranged by size:
3970 1739 971 743 251 34
BbvCI CC'TCA_GC
Cuts at: 2133 4581 2133
Size: 2448 5260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2203 2237 4005 4039 5899 5933 1903
Size: 34 266 34 1768 34 1860 34 3678
Fragments arranged by size:
3678 1860 1768 266 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5600 6810 5600
Size: 1210 6498
BclI T'GATC_A
Cuts at: 1548 3648 3867 3873 1548
Size: 2100 219 6 5383
Fragments arranged by size:
5383 2100 219 6
BfrBI ATG'CAT
Cuts at: 2214 2214
Size: 7708
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2354 2657
Size: 107 122 71 631 605 337 345 303
Cuts at: 2657 3211 6319 136
Size: 554 3108 1525
Fragments arranged by size:
3108 1525 631 605 554 345 337 303
122 107 71
BglII A'GATC_T
Cuts at: 4920 7703 4920
Size: 2783 4925
BlpI GC'TnA_GC
Cuts at: 5125 5125
Size: 7708
Bme1580I G_kGCm'C
Cuts at: 1790 2639 2693 3259 3556 3665 3896 4634 4888
Size: 849 54 566 297 109 231 738 254
Cuts at: 4888 5769 6925 1790
Size: 881 1156 2573
Fragments arranged by size:
2573 1156 881 849 738 566 297 254
231 109 54
BmgBI CAC'GTC
Cuts at: 1318 4019 1318
Size: 2701 5007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3888 4894 5439
Size: 416 53 83 399 24 2471 1006 545
Cuts at: 5439 6397 442
Size: 958 1753
Fragments arranged by size:
2471 1753 1006 958 545 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7708
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 7708
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 3159 3479 3779 6368 1239
Size: 884 1036 320 300 2589 2579
Fragments arranged by size:
2589 2579 1036 884 320 300
Bpu10I CC'TnA_GC
Cuts at: 2133 4581 2133
Size: 2448 5260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4901 4940 5833 6698 6996 4901
Size: 39 893 865 298 5613
Fragments arranged by size:
5613 893 865 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2057 2246 2706 3017 6371 882
Size: 617 558 189 460 311 3354 2219
Fragments arranged by size:
3354 2219 617 558 460 311 189
BsaAI yAC'GTr
Cuts at: 493 1204 5499 493
Size: 711 4295 2702
Fragments arranged by size:
4295 2702 711
BsaBI GATnn'nnATC
Cuts at: 3104 3872 7702 3104
Size: 768 3830 3110
Fragments arranged by size:
3830 3110 768
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3564 3852 4116 4125
Size: 53 83 186 936 2031 288 264 9
Cuts at: 4125 5515 5897 6532 275
Size: 1390 382 635 1451
Fragments arranged by size:
2031 1451 1390 936 635 382 288 264
186 83 53 9
BsaWI w'CCGG_w
Cuts at: 2681 6141 6539 6813 6960 7371 2681
Size: 3460 398 274 147 411 3018
Fragments arranged by size:
3460 3018 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3157 3187 4208 4238 1178
Size: 30 165 30 1754 30 1021 30 4648
Fragments arranged by size:
4648 1754 1021 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 3262 3956 4262 1186
Size: 1003 1073 694 306 4632
Fragments arranged by size:
4632 1073 1003 694 306
BseYI C'CCAG_C
Cuts at: 3502 4411 4804 6911 7407 3502
Size: 909 393 2107 496 3803
Fragments arranged by size:
3803 2107 909 496 393
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3019 4394 4560 4817 1163
Size: 573 1283 1375 166 257 4054
Fragments arranged by size:
4054 1375 1283 573 257 166
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3574 3955 5158 5919 6947 1540
Size: 275 186 1573 381 1203 761 1028 2301
Fragments arranged by size:
2301 1573 1203 1028 761 381 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 4634 5769 5854 6925 2127
Size: 2507 1135 85 1071 2910
Fragments arranged by size:
2910 2507 1135 1071 85
BsmI GAATG_Cn'
Cuts at: 2998 5219 5312 2998
Size: 2221 93 5394
Fragments arranged by size:
5394 2221 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3761 3761
Size: 7708
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2583 2639 2693 2864 3259 3556
Size: 399 337 456 56 54 171 395 297
Cuts at: 3556 3665 3833 3896 4047 4410 4475 4485 4634
Size: 109 168 63 151 363 65 10 149
Cuts at: 4634 4692 4811 4888 4944 5027 5769 5854 6925
Size: 58 119 77 56 83 742 85 1071
Cuts at: 6925 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 456 399 395 363 337
297 171 168 151 149 119 109 85
83 77 65 63 58 56 56 54
10
BspEI T'CCGG_A
Cuts at: 7371 7371
Size: 7708
BspHI T'CATG_A
Cuts at: 3870 5597 7327 3870
Size: 1727 1730 4251
Fragments arranged by size:
4251 1730 1727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2963 3993 4083 5063 844
Size: 2119 1030 90 980 3489
Fragments arranged by size:
3489 2119 1030 980 90
BsrBI CCG'CTC
Cuts at: 1394 1823 2015 2332 5595 1394
Size: 429 192 317 3263 3507
Fragments arranged by size:
3507 3263 429 317 192
BsrDI GCAATG_nn'
Cuts at: 65 1248 2298 2643 3271 3900 4155 4741 6203
Size: 1183 1050 345 628 629 255 586 1462
Cuts at: 6203 6377 65
Size: 174 1396
Fragments arranged by size:
1462 1396 1183 1050 629 628 586 345
255 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2651 2681 3158 3622 3912 6352 6539
Size: 110 841 30 477 464 290 2440 187
Cuts at: 6539 1700
Size: 2869
Fragments arranged by size:
2869 2440 841 477 464 290 187 110
30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7708
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7708
BssSI C'ACGA_G
Cuts at: 5768 6780 5768
Size: 1012 6696
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7708
BstBI TT'CG_AA
Cuts at: 6512 6512
Size: 7708
BstEII G'GTnAC_C
Cuts at: 1184 4396 4717 1184
Size: 3212 321 4175
Fragments arranged by size:
4175 3212 321
BstXI CCAn_nnnn'nTGG
Cuts at: 2686 2959 3349 3757 4066 4423 4699 2686
Size: 273 390 408 309 357 276 5695
Fragments arranged by size:
5695 408 390 357 309 276 273
BstYI r'GATC_y
Cuts at: 1972 2706 3858 4166 4920 4984 5038 5405 5790
Size: 734 1152 308 754 64 54 367 385
Cuts at: 5790 5807 7259 7345 7357 7703 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1152 754 734 385 367 346
308 86 64 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 3558 4356 3558
Size: 798 6910
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2067 2854 2872 2901 4161
Size: 552 939 24 39 787 18 29 1260
Cuts at: 4161 4179 4548 4698 513
Size: 18 369 150 3523
Fragments arranged by size:
3523 1260 939 787 552 369 150 39
29 24 18 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6969
Fragments arranged by size:
6969 683 56
BtsI GCAGTG_nn'
Cuts at: 2259 4173 4634 4925 5224 6018 6038 2259
Size: 1914 461 291 299 794 20 3929
Fragments arranged by size:
3929 1914 794 461 299 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7673
DraI TTT'AAA
Cuts at: 5012 5012
Size: 7708
DraIII CAC_nnn'GTG
Cuts at: 4902 4902
Size: 7708
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2551 4670 6715 809
Size: 507 1235 2119 2045 1802
Fragments arranged by size:
2119 2045 1802 1235 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2673 2833
Size: 54 1425 50 275 18 375 468 160
Cuts at: 2833 3212 3243 3667 3696 3948 4182 5155 6044
Size: 379 31 424 29 252 234 973 889
Cuts at: 6044 7673 8
Size: 1629 43
Fragments arranged by size:
1629 1425 973 889 468 424 379 375
275 252 234 160 54 50 43 31
29 18
EagI C'GGCC_G
Cuts at: 1537 1812 5155 1537
Size: 275 3343 4090
Fragments arranged by size:
4090 3343 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2244 4508 5638 1353
Size: 891 2264 1130 3423
Fragments arranged by size:
3423 2264 1130 891
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3419 6295 6669 6815 387
Size: 1064 138 1830 2876 374 146 1280
Fragments arranged by size:
2876 1830 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2125 2125
Size: 7708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2381 3159 3479 3779 4157 5771
Size: 393 491 258 778 320 300 378 1614
Cuts at: 5771 6368 7155 7423 1239
Size: 597 787 268 1524
Fragments arranged by size:
1614 1524 787 778 597 491 393 378
320 300 268 258
EcoNI CCTnn'n_nnAGG
Cuts at: 3392 4139 3392
Size: 747 6961
EcoO109I rG'GnC_Cy
Cuts at: 2356 4360 4681 4885 2356
Size: 2004 321 204 5179
Fragments arranged by size:
5179 2004 321 204
EcoRI G'AATT_C
Cuts at: 3449 5017 5395 3449
Size: 1568 378 5762
Fragments arranged by size:
5762 1568 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2764 2764
Size: 7708
FspI TGC'GCA
Cuts at: 6214 6214
Size: 7708
HaeII r_GCGC'y
Cuts at: 1678 6855 1678
Size: 5177 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2393 2413 2425 2445 2543
Size: 32 135 32 821 20 12 20 98
Cuts at: 2543 2575 3464 3496 4208 4240 4748 4780 6349
Size: 32 889 32 712 32 508 32 1569
Cuts at: 6349 6381 6423 6455 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1569 889 821 712 508 135 98
42 32 32 32 32 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2530 2605 4600 4855 5052 5298
Size: 988 281 592 75 1995 255 197 246
Cuts at: 5298 669
Size: 3079
Fragments arranged by size:
3079 1995 988 592 281 255 246 197
75
HindIII A'AGCT_T
Cuts at: 748 3007 748
Size: 2259 5449
HpaI GTT'AAC
Cuts at: 5298 5298
Size: 7708
KpnI G_GTAC'C
Cuts at: 2973 5033 2973
Size: 2060 5648
MfeI C'AATT_G
Cuts at: 5307 5307
Size: 7708
MluI A'CGCG_T
Cuts at: 7584 7584
Size: 7708
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2957 3078 4329 6822 7006 1305
Size: 1652 121 1251 2493 184 2007
Fragments arranged by size:
2493 2007 1652 1251 184 121
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 2835 3245 3698 4184
Size: 54 1425 343 843 160 410 453 486
Cuts at: 4184 10
Size: 3534
Fragments arranged by size:
3534 1425 843 486 453 410 343 160
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 2807 3347 3377 3605 3644 4100
Size: 648 1518 123 540 30 228 39 456
Cuts at: 4100 4160 4178 4697 4727 5666 6025 6184 518
Size: 60 18 519 30 939 359 159 2042
Fragments arranged by size:
2042 1518 939 648 540 519 456 359
228 159 123 60 39 30 30 18
MspA1I CmG'CkG
Cuts at: 1606 2874 3576 3841 4003 4340 4378 4559 4612
Size: 1268 702 265 162 337 38 181 53
Cuts at: 4612 5124 5801 6949 7194 1606
Size: 512 677 1148 245 2120
Fragments arranged by size:
2120 1268 1148 702 677 512 337 265
245 181 162 53 38
NaeI GCC'GGC
Cuts at: 2653 3914 2653
Size: 1261 6447
NcoI C'CATG_G
Cuts at: 513 1065 2004 2854 4161 4698 513
Size: 552 939 850 1307 537 3523
Fragments arranged by size:
3523 1307 939 850 552 537
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7708
NgoMIV G'CCGG_C
Cuts at: 2651 3912 2651
Size: 1261 6447
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7708
NotI GC'GGCC_GC
Cuts at: 5155 5155
Size: 7708
NsiI A_TGCA'T
Cuts at: 2216 2216
Size: 7708
NspI r_CATG'y
Cuts at: 1132 1229 2214 2626 3802 5066 6611 1132
Size: 97 985 412 1176 1264 1545 2229
Fragments arranged by size:
2229 1545 1264 1176 985 412 97
PasI CC'CwG_GG
Cuts at: 1348 2444 2965 3028 4345 4423 4785 1348
Size: 1096 521 63 1317 78 362 4271
Fragments arranged by size:
4271 1317 1096 521 362 78 63
PciI A'CATG_T
Cuts at: 6607 6607
Size: 7708
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4937 1108
Size: 3829 3879
PfoI T'CCnGG_A
Cuts at: 3735 3756 3735
Size: 21 7687
PmeI GTTT'AAAC
Cuts at: 5012 5012
Size: 7708
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5777 5809 7290 7322 5777
Size: 32 1481 32 6163
Fragments arranged by size:
6163 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7708
PsiI TTA'TAA
Cuts at: 5278 5278
Size: 7708
PspOMI G'GGCC_C
Cuts at: 4884 4884
Size: 7708
PstI C_TGCA'G
Cuts at: 830 2488 2944 4080 5060 830
Size: 1658 456 1136 980 3478
Fragments arranged by size:
3478 1658 1136 980 456
PvuI CG_AT'CG
Cuts at: 2001 3955 2001
Size: 1954 5754
PvuII CAG'CTG
Cuts at: 3841 4378 4559 4612 3841
Size: 537 181 53 6937
Fragments arranged by size:
6937 537 181 53
SacI G_AGCT'C
Cuts at: 2127 2127
Size: 7708
SacII CC_GC'GG
Cuts at: 2875 2875
Size: 7708
SalI G'TCGA_C
Cuts at: 1936 5050 1936
Size: 3114 4594
SapI GCTCTTCn'nnn_
Cuts at: 4508 4508
Size: 7708
SbfI CC_TGCA'GG
Cuts at: 5060 5060
Size: 7708
ScaI AGT'ACT
Cuts at: 5956 7668 5956
Size: 1712 5996
SfcI C'TryA_G
Cuts at: 826 1045 2484 2940 4076 4603 5056 6191 6872
Size: 219 1439 456 1136 527 453 1135 681
Cuts at: 6872 7063 826
Size: 191 1471
Fragments arranged by size:
1471 1439 1136 1135 681 527 456 453
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7708
SgrAI Cr'CCGG_yG
Cuts at: 1700 2681 1700
Size: 981 6727
SmaI CCC'GGG
Cuts at: 1872 3518 3605 3704 5035 1872
Size: 1646 87 99 1331 4545
Fragments arranged by size:
4545 1646 1331 99 87
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4916 4955 5812 6713 6975 820
Size: 197 926 2973 39 857 901 262 1553
Fragments arranged by size:
2973 1553 926 901 857 262 197 39
SnaBI TAC'GTA
Cuts at: 493 5499 493
Size: 5006 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7708
SphI G_CATG'C
Cuts at: 1132 2214 3802 5066 1132
Size: 1082 1588 1264 3774
Fragments arranged by size:
3774 1588 1264 1082
SspI AAT'ATT
Cuts at: 5 52 5632 5
Size: 47 5580 2081
Fragments arranged by size:
5580 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 1517
Size: 1143 6565
StyI C'CwwG_G
Cuts at: 513 1065 2004 2661 2787 2854 2907 3276 4161
Size: 552 939 657 126 67 53 369 885
Cuts at: 4161 4476 4698 5147 513
Size: 315 222 449 3074
Fragments arranged by size:
3074 939 885 657 552 449 369 315
222 126 67 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 4375 4415 4526 4661 4777 4913
Size: 1255 2196 599 40 111 135 116 136
Cuts at: 4913 5735 5752 5905 6090 6535 325
Size: 822 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 822 599 445 185 153
136 135 116 111 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5954 7666 96
Size: 274 80 33 51 1443 3977 1712 138
Fragments arranged by size:
3977 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3157 3189 7668 7700 3157
Size: 32 4479 32 3165
Fragments arranged by size:
4479 3165 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2438 2477 2849 1316
Size: 1122 39 372 6175
Fragments arranged by size:
6175 1122 372 39
XbaI T'CTAG_A
Cuts at: 5044 5044
Size: 7708
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2683 2311
Size: 372 7336
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7708
XmaI C'CCGG_G
Cuts at: 1870 3516 3603 3702 5033 1870
Size: 1646 87 99 1331 4545
Fragments arranged by size:
4545 1646 1331 99 87
XmnI GAAnn'nnTTC
Cuts at: 2934 3146 3365 3449 4280 5837 2934
Size: 212 219 84 831 1557 4805
Fragments arranged by size:
4805 1557 831 219 212 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5516 276
Size: 53 83 186 936 3982 2468
Fragments arranged by size:
3982 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV
FseI FspAI KasI NarI NruI PacI PmlI PpuMI
PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI
SwaI