Restriction map for
pFN21AE0050
(Circular) (Six-base) MAPSORT of: pFN21AE0050.seq Check: 5849 from: 1 to: 6112
pFN21AE0050 6112 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2497 3378 3455 1937
Size: 560 881 77 4594
Fragments arranged by size:
4594 881 560 77
Acc65I G'GTAC_C
Cuts at: 3433 3433
Size: 6112
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2334 3340 4175 5559 5827 1632
Size: 702 1006 835 1384 268 1917
Fragments arranged by size:
1917 1384 1006 835 702 268
AfeI AGC'GCT
Cuts at: 2718 2718
Size: 6112
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 2912 5011 5988 1203
Size: 1709 2099 977 1327
Fragments arranged by size:
2099 1709 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 2685 3273 3357 4841 2685
Size: 588 84 1484 3956
Fragments arranged by size:
3956 1484 588 84
AleI CACnn'nnGTG
Cuts at: 1166 2643 1166
Size: 1477 4635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AlwNI CAG_nnn'CTG
Cuts at: 3855 5427 3855
Size: 1572 4540
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 2096 2382 3421 3647 3799 5908 1493
Size: 603 286 1039 226 152 2109 1697
Fragments arranged by size:
2109 1697 1039 603 286 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 3437 1870
Size: 73 1494 4545
Fragments arranged by size:
4545 1494 73
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3433 4888 618
Size: 325 217 627 1646 1455 1842
Fragments arranged by size:
1842 1646 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3431 1391
Size: 2040 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2018 928
Size: 971 119 5022
Fragments arranged by size:
5022 971 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3253 3287 4303 4337 1903
Size: 34 1316 34 1016 34 3678
Fragments arranged by size:
3678 1316 1016 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 2224 1548
Size: 676 5436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4723 136
Size: 107 122 71 631 605 3051 1525
Fragments arranged by size:
3051 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 4173 5329 1790
Size: 2383 1156 2573
Fragments arranged by size:
2573 2383 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3843 4801 442
Size: 416 53 83 399 24 2426 958 1753
Fragments arranged by size:
2426 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2748 2832 2916 3000 3168 3252 3336 4772
Size: 1509 84 84 84 168 84 84 1436
Cuts at: 4772 1239
Size: 2579
Fragments arranged by size:
2579 1509 1436 168 84 84 84 84
84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4237 5102 5400 4237
Size: 865 298 4949
Fragments arranged by size:
4949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4775 882
Size: 617 3276 2219
Fragments arranged by size:
3276 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3919 4301 4936 275
Size: 53 83 186 936 2386 382 635 1451
Fragments arranged by size:
2386 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4545 4943 5217 5364 5775 4545
Size: 398 274 147 411 4882
Fragments arranged by size:
4882 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5887
Fragments arranged by size:
5887 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6112
BseYI C'CCAG_C
Cuts at: 5315 5811 5315
Size: 496 5616
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5539
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4173 4258 5329 4173
Size: 85 1071 4956
Fragments arranged by size:
4956 1071 85
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3431 4173 4258 5329 1391
Size: 399 1641 742 85 1071 2174
Fragments arranged by size:
2174 1641 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 4001 5731 4001
Size: 1730 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3467 844
Size: 2623 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2063 4607 4781 65
Size: 1183 815 2544 174 1396
Fragments arranged by size:
2544 1396 1183 815 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4756 4943 1700
Size: 110 2946 187 2869
Fragments arranged by size:
2946 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 4172 5184 4172
Size: 1012 5100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6112
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107
Size: 1470 367 385 17 1452 86 12 346
Cuts at: 6107 1972
Size: 1977
Fragments arranged by size:
1977 1470 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2498 3379 2498
Size: 881 5231
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3270 487
Size: 683 56 2044 3329
Fragments arranged by size:
3329 2044 683 56
BtsI GCAGTG_nn'
Cuts at: 3628 4422 4442 3628
Size: 794 20 5298
Fragments arranged by size:
5298 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2020 2079 5119 809
Size: 507 704 59 3040 1802
Fragments arranged by size:
3040 1802 704 507 59
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2167 3559 4448
Size: 54 1425 50 275 18 337 1392 889
Cuts at: 4448 6077 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1392 889 337 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2556 2708 2997 4042 1353
Size: 1203 152 289 1045 3423
Fragments arranged by size:
3423 1203 1045 289 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 2748 2832 2916 3000 3168 3252
Size: 393 702 414 84 84 84 168 84
Cuts at: 3252 3336 3340 4175 4772 5559 5827 1239
Size: 84 4 835 597 787 268 1524
Fragments arranged by size:
1524 835 787 702 597 414 393 268
168 84 84 84 84 84 4
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2279 2279
Size: 6112
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 2720 5259 1678
Size: 1042 2539 2531
Fragments arranged by size:
2539 2531 1042
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2671 2703 3259 3291 3343
Size: 32 135 32 1099 32 556 32 52
Cuts at: 3343 3375 4753 4785 4827 4859 1373
Size: 32 1378 32 42 32 2626
Fragments arranged by size:
2626 1378 1099 556 135 52 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2025 3456 3702 669
Size: 988 281 87 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246 87
HindIII A'AGCT_T
Cuts at: 748 2272 748
Size: 1524 4588
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KpnI G_GTAC'C
Cuts at: 3437 3437
Size: 6112
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5226 5410 1305
Size: 3921 184 2007
Fragments arranged by size:
3921 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2169 10
Size: 54 1425 343 337 3953
Fragments arranged by size:
3953 1425 343 337 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2458 2643 2827 3163 4070 4429 4588
Size: 648 1292 185 184 336 907 359 159
Cuts at: 4588 518
Size: 2042
Fragments arranged by size:
2042 1292 907 648 359 336 185 184
159
MspA1I CmG'CkG
Cuts at: 1606 3528 4205 5353 5598 1606
Size: 1922 677 1148 245 2120
Fragments arranged by size:
2120 1922 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5560
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2237 2916 3470 5015 1132
Size: 97 1008 679 554 1545 2229
Fragments arranged by size:
2229 1545 1008 679 554 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 2912 5011 2912
Size: 2099 4013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6112
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3293 3682 3293
Size: 389 5723
PstI C_TGCA'G
Cuts at: 830 3464 830
Size: 2634 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 3460 4595 5276 5467 826
Size: 219 2415 1135 681 191 1471
Fragments arranged by size:
2415 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4216 5117 5379 820
Size: 197 926 2273 901 262 1553
Fragments arranged by size:
2273 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 2237 3470 1132
Size: 1105 1233 3774
Fragments arranged by size:
3774 1233 1105
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 3551 513
Size: 552 2486 3074
Fragments arranged by size:
3074 2486 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2194 3263 4139 4156 4309 4494 4939
Size: 1255 614 1069 876 17 153 185 445
Cuts at: 4939 325
Size: 1498
Fragments arranged by size:
1498 1255 1069 876 614 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2210 2467 4358
Size: 274 80 33 51 1443 233 257 1891
Cuts at: 4358 6070 96
Size: 1712 138
Fragments arranged by size:
1891 1712 1443 274 257 233 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3046 3078 6072 6104 3046
Size: 32 2994 32 3054
Fragments arranged by size:
3054 2994 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2017 1316
Size: 701 5411
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 2428 4241 2428
Size: 1813 4299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI