Restriction map for
pFN21AE0048
(Circular) (Six-base) MAPSORT of: pFN21AE0048.seq Check: 3020 from: 1 to: 6271
pFN21AE0048 6271 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4081 278
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 3614 1937
Size: 1149 528 4594
Fragments arranged by size:
4594 1149 528
Acc65I G'GTAC_C
Cuts at: 2239 3592 2239
Size: 1353 4918
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2337 2668 2843 3195 3204 4334 5718 5986
Size: 705 331 175 352 9 1130 1384 268
Cuts at: 5986 1632
Size: 1917
Fragments arranged by size:
1917 1384 1130 705 352 331 268 175
9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 3414 5170 6147 1203
Size: 2211 1756 977 1327
Fragments arranged by size:
2211 1756 1327 977
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6271
AlwNI CAG_nnn'CTG
Cuts at: 2996 3176 4014 5586 2996
Size: 180 838 1572 3681
Fragments arranged by size:
3681 1572 838 180
ApaLI G'TGCA_C
Cuts at: 4328 5484 4328
Size: 1156 5115
ApoI r'AATT_y
Cuts at: 1493 2695 3004 3580 3806 3958 6067 1493
Size: 1202 309 576 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 576 309 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4825 160
Size: 872 3793 1606
Fragments arranged by size:
3793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 3420 3596 1870
Size: 73 1477 176 4545
Fragments arranged by size:
4545 1477 176 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2601 2609 2634 2642 2601
Size: 8 25 8 6230
Fragments arranged by size:
6230 25 8 8
BamHI G'GATC_C
Cuts at: 2288 3601 3968 2288
Size: 1313 367 4591
Fragments arranged by size:
4591 1313 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2239 3407 3592 5047 618
Size: 325 217 627 452 1168 185 1455 1842
Fragments arranged by size:
1842 1455 1168 627 452 325 217 185
BanII G_rGCy'C
Cuts at: 1391 3035 3590 1391
Size: 1644 555 4072
Fragments arranged by size:
4072 1644 555
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5300
BbvCI CC'TCA_GC
Cuts at: 2060 2060
Size: 6271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4462 4496 1903
Size: 34 2525 34 3678
Fragments arranged by size:
3678 2525 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2637 4163 5373 2637
Size: 1526 1210 3535
Fragments arranged by size:
3535 1526 1210
BclI T'GATC_A
Cuts at: 1548 2392 2554 1548
Size: 844 162 5265
Fragments arranged by size:
5265 844 162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4882 136
Size: 107 122 71 631 605 3210 1525
Fragments arranged by size:
3210 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6266 6266
Size: 6271
BlpI GC'TnA_GC
Cuts at: 3688 3688
Size: 6271
Bme1580I G_kGCm'C
Cuts at: 1790 4332 5488 1790
Size: 2542 1156 2573
Fragments arranged by size:
2573 2542 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6271
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3146 4002 4960
Size: 416 53 83 399 24 1729 856 958
Cuts at: 4960 442
Size: 1753
Fragments arranged by size:
1753 1729 958 856 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3411 4931 1239
Size: 2172 1520 2579
Fragments arranged by size:
2579 2172 1520
Bpu10I CC'TnA_GC
Cuts at: 2060 2060
Size: 6271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2287 4396 5261 5559 2287
Size: 2109 865 298 2999
Fragments arranged by size:
2999 2109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4934 882
Size: 617 3435 2219
Fragments arranged by size:
3435 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4062 493
Size: 711 2858 2702
Fragments arranged by size:
2858 2702 711
BsaBI GATnn'nnATC
Cuts at: 6265 6265
Size: 6271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4078 4460 5095 275
Size: 53 83 186 936 2545 382 635 1451
Fragments arranged by size:
2545 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4704 5102 5376 5523 5934 4704
Size: 398 274 147 411 5041
Fragments arranged by size:
5041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6046
Fragments arranged by size:
6046 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2895 3408 1186
Size: 1709 513 4049
Fragments arranged by size:
4049 1709 513
BseYI C'CCAG_C
Cuts at: 5474 5970 5474
Size: 496 5775
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5698
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3721 4482 5510 1540
Size: 275 186 1720 761 1028 2301
Fragments arranged by size:
2301 1720 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4332 4417 5488 4332
Size: 85 1071 5115
Fragments arranged by size:
5115 1071 85
BsmI GAATG_Cn'
Cuts at: 3782 3875 3782
Size: 93 6178
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3035 3590 4332 4417 5488 1391
Size: 399 1245 555 742 85 1071 2174
Fragments arranged by size:
2174 1245 1071 742 555 399 85
BspEI T'CCGG_A
Cuts at: 5934 5934
Size: 6271
BspHI T'CATG_A
Cuts at: 4160 5890 4160
Size: 1730 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3626 844
Size: 2782 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4158 1394
Size: 429 2335 3507
Fragments arranged by size:
3507 2335 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2642 3075 4766 4940 65
Size: 1183 1394 433 1691 174 1396
Fragments arranged by size:
1691 1396 1394 1183 433 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4915 5102 1700
Size: 110 3105 187 2869
Fragments arranged by size:
3105 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2617 96
Size: 2521 3750
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 4331 5343 4331
Size: 1012 5259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6271
BstBI TT'CG_AA
Cuts at: 5075 5075
Size: 6271
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6271
BstXI CCAn_nnnn'nTGG
Cuts at: 2886 2886
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 2288 3484 3601 3968 4353 4370 5822 5908
Size: 316 1196 117 367 385 17 1452 86
Cuts at: 5908 5920 6266 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1196 385 367 346 316 117
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4780
Fragments arranged by size:
4780 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 2920 3787 4581 4601 2920
Size: 867 794 20 4590
Fragments arranged by size:
4590 867 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6236
DraI TTT'AAA
Cuts at: 3575 3575
Size: 6271
DraIII CAC_nnn'GTG
Cuts at: 2027 2915 2027
Size: 888 5383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5278 809
Size: 507 3962 1802
Fragments arranged by size:
3962 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2888 3541
Size: 54 1425 50 275 18 185 873 653
Cuts at: 3541 3718 4607 6236 8
Size: 177 889 1629 43
Fragments arranged by size:
1629 1425 889 873 653 275 185 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3718 1537
Size: 275 1906 4090
Fragments arranged by size:
4090 1906 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 2455 2473 3249 4201 1353
Size: 744 358 18 776 952 3423
Fragments arranged by size:
3423 952 776 744 358 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4858 5232 5378 387
Size: 1064 138 3269 374 146 1280
Fragments arranged by size:
3269 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2337 2668 2843 3195 3204 3411 4334
Size: 393 705 331 175 352 9 207 923
Cuts at: 4334 4931 5718 5986 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 923 787 705 597 393 352 331
268 207 175 9
EcoO109I rG'GnC_Cy
Cuts at: 2900 2900
Size: 6271
EcoRI G'AATT_C
Cuts at: 3580 3958 3580
Size: 378 5893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2663 3251 2663
Size: 588 5683
FspI TGC'GCA
Cuts at: 4777 4777
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 5418 1678
Size: 3740 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2935 2967 3092
Size: 32 135 32 611 32 720 32 125
Cuts at: 3092 3124 3343 3375 4912 4944 4986 5018 1373
Size: 32 219 32 1537 32 42 32 2626
Fragments arranged by size:
2626 1537 720 611 219 135 125 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2312 3615 3861 669
Size: 988 281 374 1303 246 3079
Fragments arranged by size:
3079 1303 988 374 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6271
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KpnI G_GTAC'C
Cuts at: 2243 3596 2243
Size: 1353 4918
MfeI C'AATT_G
Cuts at: 3870 3870
Size: 6271
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2345 5385 5569 1305
Size: 1040 3040 184 2007
Fragments arranged by size:
3040 2007 1040 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2017 2890 3543 10
Size: 54 1425 343 185 873 653 2738
Fragments arranged by size:
2738 1425 873 653 343 185 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 3413 4229 4588 4747 518
Size: 648 1087 1160 816 359 159 2042
Fragments arranged by size:
2042 1160 1087 816 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3687 4364 5512 5757 1606
Size: 2081 677 1148 245 2120
Fragments arranged by size:
2120 2081 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4780
Fragments arranged by size:
4780 939 552
NdeI CA'TA_TG
Cuts at: 387 2524 387
Size: 2137 4134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6271
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 3418 3629 5174 1132
Size: 97 2189 211 1545 2229
Fragments arranged by size:
2229 2189 1545 211 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 3414 5170 3414
Size: 1756 4515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3017 1108
Size: 1909 4362
PfoI T'CCnGG_A
Cuts at: 2291 3388 2291
Size: 1097 5174
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4340 4372 5853 5885 4340
Size: 32 1481 32 4726
Fragments arranged by size:
4726 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 3841 3841
Size: 6271
PstI C_TGCA'G
Cuts at: 830 3623 830
Size: 2793 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SbfI CC_TGCA'GG
Cuts at: 3623 3623
Size: 6271
ScaI AGT'ACT
Cuts at: 4519 6231 4519
Size: 1712 4559
SfcI C'TryA_G
Cuts at: 826 1045 3619 4754 5435 5626 826
Size: 219 2574 1135 681 191 1471
Fragments arranged by size:
2574 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2302 4375 5276 5538 820
Size: 197 926 359 2073 901 262 1553
Fragments arranged by size:
2073 1553 926 901 359 262 197
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6271
SphI G_CATG'C
Cuts at: 1132 3629 1132
Size: 2497 3774
SspI AAT'ATT
Cuts at: 5 52 2678 4195 5
Size: 47 2626 1517 2081
Fragments arranged by size:
2626 2081 1517 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6271
StyI C'CwwG_G
Cuts at: 513 1065 2004 2065 3116 3710 513
Size: 552 939 61 1051 594 3074
Fragments arranged by size:
3074 1051 939 594 552 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3425 4298 4315 4468 4653 5098 325
Size: 1255 1845 873 17 153 185 445 1498
Fragments arranged by size:
1845 1498 1255 873 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2617 4517 6229
Size: 274 80 33 51 1443 640 1900 1712
Cuts at: 6229 96
Size: 138
Fragments arranged by size:
1900 1712 1443 640 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6231 6263 6231
Size: 32 6239
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6271
XbaI T'CTAG_A
Cuts at: 3074 3607 3074
Size: 533 5738
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6271
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 4400 4400
Size: 6271
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4079 276
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI