Restriction map for
pFN21AE0041
(Circular) (Six-base) MAPSORT of: pFN21AE0041.seq Check: 9262 from: 1 to: 6265
pFN21AE0041 6265 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 3002 4328 5712 5980 1632
Size: 602 768 1326 1384 268 1917
Fragments arranged by size:
1917 1384 1326 768 602 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 3189 3218 5164 6141 1203
Size: 1986 29 1946 977 1327
Fragments arranged by size:
1986 1946 1327 977 29
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 3299 3377 1166
Size: 2133 78 4054
Fragments arranged by size:
4054 2133 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2052 2084 2052
Size: 32 6233
AlwNI CAG_nnn'CTG
Cuts at: 2140 4008 5580 2140
Size: 1868 1572 2825
Fragments arranged by size:
2825 1868 1572
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 2053 2403 2747 3038 3341 3574 3800 3952
Size: 560 350 344 291 303 233 226 152
Cuts at: 3952 6061 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 560 350 344 303 291 233
226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2268 3590 1870
Size: 73 325 1322 4545
Fragments arranged by size:
4545 1322 325 73
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2157 3586 5041 618
Size: 325 217 627 370 1429 1455 1842
Fragments arranged by size:
1842 1455 1429 627 370 325 217
BanII G_rGCy'C
Cuts at: 1391 2677 3584 1391
Size: 1286 907 4072
Fragments arranged by size:
4072 1286 907
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2710 3192 3457 928
Size: 971 811 482 265 3736
Fragments arranged by size:
3736 971 811 482 265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BfrBI ATG'CAT
Cuts at: 2478 2478
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4876 136
Size: 107 122 71 631 605 3204 1525
Fragments arranged by size:
3204 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2501 3020 4326 5482 1790
Size: 370 341 519 1306 1156 2573
Fragments arranged by size:
2573 1306 1156 519 370 341
BmgBI CAC'GTC
Cuts at: 1318 2602 1318
Size: 1284 4981
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3996 4954 442
Size: 416 53 83 399 24 2579 958 1753
Fragments arranged by size:
2579 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2278 2522 2929 4925 1239
Size: 1039 244 407 1996 2579
Fragments arranged by size:
2579 1996 1039 407 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2190 4390 5255 5553 2190
Size: 2200 865 298 2902
Fragments arranged by size:
2902 2200 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2890 4056 493
Size: 711 1686 1166 2702
Fragments arranged by size:
2702 1686 1166 711
BsaBI GATnn'nnATC
Cuts at: 2522 6259 2522
Size: 3737 2528
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2052 2082 1178
Size: 30 165 30 649 30 5361
Fragments arranged by size:
5361 649 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3218 1186
Size: 2032 4233
BseYI C'CCAG_C
Cuts at: 2579 2586 3254 5468 5964 2579
Size: 7 668 2214 496 2880
Fragments arranged by size:
2880 2214 668 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5692
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4326 4411 5482 4326
Size: 85 1071 5109
Fragments arranged by size:
5109 1071 85
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2501 2677 3020 3584 4326 4411
Size: 399 370 341 176 343 564 742 85
Cuts at: 4411 5482 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 564 399 370 343 341
176 85
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 3270 4154 5884 3270
Size: 884 1730 3651
Fragments arranged by size:
3651 1730 884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2017 3620 844
Size: 1173 1603 3489
Fragments arranged by size:
3489 1603 1173
BsrBI CCG'CTC
Cuts at: 1394 1823 2105 4152 1394
Size: 429 282 2047 3507
Fragments arranged by size:
3507 2047 429 282
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4909 5096 1700
Size: 110 3099 187 2869
Fragments arranged by size:
3099 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6265
BstBI TT'CG_AA
Cuts at: 2701 5069 2701
Size: 2368 3897
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 3595 3962 4347 4364 5816 5902 5914 6260
Size: 1623 367 385 17 1452 86 12 346
Cuts at: 6260 1972
Size: 1977
Fragments arranged by size:
1977 1623 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4774
Fragments arranged by size:
4774 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 3781 4575 4595 3781
Size: 794 20 5451
Fragments arranged by size:
5451 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 2692 3569 2692
Size: 877 5388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3712 4601 6230
Size: 54 1425 50 275 18 1882 889 1629
Cuts at: 6230 8
Size: 43
Fragments arranged by size:
1882 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2280 2755 4195 1353
Size: 759 168 475 1440 3423
Fragments arranged by size:
3423 1440 759 475 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4852 5226 5372 387
Size: 1064 138 3263 374 146 1280
Fragments arranged by size:
3263 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2278 2522 2929 3002 4328 4925
Size: 393 602 44 244 407 73 1326 597
Cuts at: 4925 5712 5980 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1326 787 602 597 407 393 268
244 73 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2618 2639 2618
Size: 21 6244
EcoRI G'AATT_C
Cuts at: 3038 3574 3952 3038
Size: 536 378 5351
Fragments arranged by size:
5351 536 378
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 5412 1678
Size: 3734 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 2542 2574 3427
Size: 32 135 32 701 32 237 32 853
Cuts at: 3427 3459 4906 4938 4980 5012 1373
Size: 32 1447 32 42 32 2626
Fragments arranged by size:
2626 1447 853 701 237 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3067 3285 5379 5563 1305
Size: 1762 218 2094 184 2007
Fragments arranged by size:
2094 2007 1762 218 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4443
Fragments arranged by size:
4443 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2428 3206 3299 3377 4223 4582 4741
Size: 648 1262 778 93 78 846 359 159
Cuts at: 4741 518
Size: 2042
Fragments arranged by size:
2042 1262 846 778 648 359 159 93
78
MspA1I CmG'CkG
Cuts at: 1606 2339 3681 4358 5506 5751 1606
Size: 733 1342 677 1148 245 2120
Fragments arranged by size:
2120 1342 1148 733 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4774
Fragments arranged by size:
4774 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NsiI A_TGCA'T
Cuts at: 2480 2480
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2482 3193 3222 3623 5168 1132
Size: 97 1253 711 29 401 1545 2229
Fragments arranged by size:
2229 1545 1253 711 401 97 29
PacI TTA_AT'TAA
Cuts at: 2184 2184
Size: 6265
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 3189 3218 5164 3189
Size: 29 1946 4290
Fragments arranged by size:
4290 1946 29
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2941 1108
Size: 1833 4432
PfoI T'CCnGG_A
Cuts at: 2634 2634
Size: 6265
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2052 2084 4334 4366 5847 5879 2052
Size: 32 2250 32 1481 32 2438
Fragments arranged by size:
2438 2250 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2215 1613
Size: 602 5663
PsiI TTA'TAA
Cuts at: 3069 3835 3069
Size: 766 5499
PstI C_TGCA'G
Cuts at: 830 2036 2622 3617 830
Size: 1206 586 995 3478
Fragments arranged by size:
3478 1206 995 586
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2339 2339
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SapI GCTCTTCn'nnn_
Cuts at: 2112 2112
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SexAI A'CCwGG_T
Cuts at: 2012 2012
Size: 6265
SfcI C'TryA_G
Cuts at: 826 1045 2032 2618 3613 4748 5429 5620 826
Size: 219 987 586 995 1135 681 191 1471
Fragments arranged by size:
1471 1135 995 987 681 586 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2205 2268 4369 5270 5532 820
Size: 197 926 262 63 2101 901 262 1553
Fragments arranged by size:
2101 1553 926 901 262 262 197 63
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 3148 4189 5
Size: 47 3096 1041 2081
Fragments arranged by size:
3096 2081 1041 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6265
StyI C'CwwG_G
Cuts at: 513 1065 2004 3399 3704 513
Size: 552 939 1395 305 3074
Fragments arranged by size:
3074 1395 939 552 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 3570 4292 4309 4462 4647 5092
Size: 1255 1020 970 722 17 153 185 445
Cuts at: 5092 325
Size: 1498
Fragments arranged by size:
1498 1255 1020 970 722 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2592 4511 6223
Size: 274 80 33 51 1443 615 1919 1712
Cuts at: 6223 96
Size: 138
Fragments arranged by size:
1919 1712 1443 615 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6225 6257 6225
Size: 32 6233
Tth111I GACn'n_nGTC
Cuts at: 1316 2437 1316
Size: 1121 5144
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3301 3301
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 2268 1943
Size: 325 5940
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI