Restriction map for
pFN21AE0039
(Circular) (Six-base) MAPSORT of: pFN21AE0039.seq Check: 8686 from: 1 to: 6244
pFN21AE0039 6244 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4054 278
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3587 1937
Size: 1650 4594
Acc65I G'GTAC_C
Cuts at: 3565 3565
Size: 6244
AclI AA'CG_TT
Cuts at: 1563 4371 4744 1563
Size: 2808 373 3063
Fragments arranged by size:
3063 2808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4307 5691 5959 1632
Size: 2675 1384 268 1917
Fragments arranged by size:
2675 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6047
AflIII A'CryG_T
Cuts at: 1203 3118 5143 6120 1203
Size: 1915 2025 977 1327
Fragments arranged by size:
2025 1915 1327 977
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 4973 4973
Size: 6244
AleI CACnn'nnGTG
Cuts at: 1166 2272 1166
Size: 1106 5138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 6212
AlwNI CAG_nnn'CTG
Cuts at: 2228 3987 5559 2228
Size: 1759 1572 2913
Fragments arranged by size:
2913 1759 1572
ApaLI G'TGCA_C
Cuts at: 4301 5457 4301
Size: 1156 5088
ApoI r'AATT_y
Cuts at: 1493 2190 2438 2654 3553 3779 3931 6040 1493
Size: 697 248 216 899 226 152 2109 1697
Fragments arranged by size:
2109 1697 899 697 248 226 216 152
AseI AT'TA_AT
Cuts at: 160 1032 2954 4798 160
Size: 872 1922 1844 1606
Fragments arranged by size:
1922 1844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 2490 3569 1870
Size: 73 547 1079 4545
Fragments arranged by size:
4545 1079 547 73
BamHI G'GATC_C
Cuts at: 2728 3574 3941 2728
Size: 846 367 5031
Fragments arranged by size:
5031 846 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2281 2907 3565 5020 618
Size: 325 217 627 494 626 658 1455 1842
Fragments arranged by size:
1842 1455 658 627 626 494 325 217
BanII G_rGCy'C
Cuts at: 1391 2676 3563 1391
Size: 1285 887 4072
Fragments arranged by size:
4072 1285 887
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2270 928
Size: 971 371 4902
Fragments arranged by size:
4902 971 371
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4435 4469 1903
Size: 34 2498 34 3678
Fragments arranged by size:
3678 2498 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3281 4136 5346 3281
Size: 855 1210 4179
Fragments arranged by size:
4179 1210 855
BclI T'GATC_A
Cuts at: 1548 2689 3054 1548
Size: 1141 365 4738
Fragments arranged by size:
4738 1141 365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2301 4855 136
Size: 107 122 71 631 605 629 2554 1525
Fragments arranged by size:
2554 1525 631 629 605 122 107 71
BglII A'GATC_T
Cuts at: 6239 6239
Size: 6244
BlpI GC'TnA_GC
Cuts at: 2808 3661 2808
Size: 853 5391
Bme1580I G_kGCm'C
Cuts at: 1790 4305 5461 1790
Size: 2515 1156 2573
Fragments arranged by size:
2573 2515 1156
BmgBI CAC'GTC
Cuts at: 1318 3119 1318
Size: 1801 4443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2678 3975 4933
Size: 416 53 83 399 24 1261 1297 958
Cuts at: 4933 442
Size: 1753
Fragments arranged by size:
1753 1297 1261 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3214 4904 1239
Size: 1975 1690 2579
Fragments arranged by size:
2579 1975 1690
Bpu10I CC'TnA_GC
Cuts at: 2103 2103
Size: 6244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 4369 5234 5532 2273
Size: 2096 865 298 2985
Fragments arranged by size:
2985 2096 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4907 882
Size: 617 3408 2219
Fragments arranged by size:
3408 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4035 493
Size: 711 2831 2702
Fragments arranged by size:
2831 2702 711
BsaBI GATnn'nnATC
Cuts at: 6238 6238
Size: 6244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4051 4433 5068 275
Size: 53 83 186 936 2518 382 635 1451
Fragments arranged by size:
2518 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2067 2362 4677 5075 5349 5496 5907 2067
Size: 295 2315 398 274 147 411 2404
Fragments arranged by size:
2404 2315 411 398 295 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6019
Fragments arranged by size:
6019 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3406 1186
Size: 2220 4024
BseYI C'CCAG_C
Cuts at: 5447 5943 5447
Size: 496 5748
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5671
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3694 4455 5483 1540
Size: 275 186 1693 761 1028 2301
Fragments arranged by size:
2301 1693 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4305 4390 5461 4305
Size: 85 1071 5088
Fragments arranged by size:
5088 1071 85
BsmI GAATG_Cn'
Cuts at: 3755 3848 3755
Size: 93 6151
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2676 3563 4305 4390 5461 1391
Size: 399 886 887 742 85 1071 2174
Fragments arranged by size:
2174 1071 887 886 742 399 85
BspEI T'CCGG_A
Cuts at: 2067 2362 5907 2067
Size: 295 3545 2404
Fragments arranged by size:
3545 2404 295
BspHI T'CATG_A
Cuts at: 2259 2472 2650 3372 4133 5863 2259
Size: 213 178 722 761 1730 2640
Fragments arranged by size:
2640 1730 761 722 213 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2812 3599 844
Size: 1968 787 3489
Fragments arranged by size:
3489 1968 787
BsrBI CCG'CTC
Cuts at: 1394 1823 4131 1394
Size: 429 2308 3507
Fragments arranged by size:
3507 2308 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4739 4913 65
Size: 1183 3491 174 1396
Fragments arranged by size:
3491 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4888 5075 1700
Size: 110 3078 187 2869
Fragments arranged by size:
3078 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6244
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6244
BssSI C'ACGA_G
Cuts at: 2373 4304 5316 2373
Size: 1931 1012 3301
Fragments arranged by size:
3301 1931 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2378 3189 794
Size: 1584 811 3849
Fragments arranged by size:
3849 1584 811
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6244
BstYI r'GATC_y
Cuts at: 1972 2728 2919 3574 3941 4326 4343 5795 5881
Size: 756 191 655 367 385 17 1452 86
Cuts at: 5881 5893 6239 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 756 655 385 367 346 191
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2796 2796
Size: 6244
BtgI C'CryG_G
Cuts at: 513 1065 2497 3219 3319 513
Size: 552 1432 722 100 3438
Fragments arranged by size:
3438 1432 722 552 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5505
Fragments arranged by size:
5505 683 56
BtsI GCAGTG_nn'
Cuts at: 3760 4554 4574 3760
Size: 794 20 5430
Fragments arranged by size:
5430 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 2899 3034 3548 2899
Size: 135 514 5595
Fragments arranged by size:
5595 514 135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5251 809
Size: 507 3935 1802
Fragments arranged by size:
3935 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2542 2969 3691
Size: 54 1425 50 275 18 712 427 722
Cuts at: 3691 4580 6209 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 722 712 427 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3691 1537
Size: 275 1879 4090
Fragments arranged by size:
4090 1879 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4174 1353
Size: 2821 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4831 5205 5351 387
Size: 1064 138 3242 374 146 1280
Fragments arranged by size:
3242 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3214 4307 4904 5691 5959 1239
Size: 393 1582 1093 597 787 268 1524
Fragments arranged by size:
1582 1524 1093 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3553 3931 3553
Size: 378 5866
EcoRV GAT'ATC
Cuts at: 2761 2932 3433 2761
Size: 171 501 5572
Fragments arranged by size:
5572 501 171
FspI TGC'GCA
Cuts at: 4750 4750
Size: 6244
HaeII r_GCGC'y
Cuts at: 1678 2014 5391 1678
Size: 336 3377 2531
Fragments arranged by size:
3377 2531 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3054 3086 3460 3492 4885
Size: 32 135 32 1482 32 374 32 1393
Cuts at: 4885 4917 4959 4991 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1482 1393 374 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3588 3834 669
Size: 988 281 1650 246 3079
Fragments arranged by size:
3079 1650 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3490 748
Size: 2742 3502
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KpnI G_GTAC'C
Cuts at: 3569 3569
Size: 6244
MfeI C'AATT_G
Cuts at: 3843 3843
Size: 6244
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5358 5542 1305
Size: 4053 184 2007
Fragments arranged by size:
4053 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2544 2971 10
Size: 54 1425 343 712 427 3283
Fragments arranged by size:
3283 1425 712 427 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2272 2589 2685 2838 4202 4561 4720
Size: 648 1106 317 96 153 1364 359 159
Cuts at: 4720 518
Size: 2042
Fragments arranged by size:
2042 1364 1106 648 359 317 159 153
96
MspA1I CmG'CkG
Cuts at: 1606 2984 3660 4337 5485 5730 1606
Size: 1378 676 677 1148 245 2120
Fragments arranged by size:
2120 1378 1148 677 676 245
NcoI C'CATG_G
Cuts at: 513 1065 2497 3219 513
Size: 552 1432 722 3538
Fragments arranged by size:
3538 1432 722 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 3691 3691
Size: 6244
NspI r_CATG'y
Cuts at: 1132 1229 3602 5147 1132
Size: 97 2373 1545 2229
Fragments arranged by size:
2373 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2315 1348
Size: 967 5277
PciI A'CATG_T
Cuts at: 5143 5143
Size: 6244
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3220 1108
Size: 2112 4132
PfoI T'CCnGG_A
Cuts at: 3112 3259 3112
Size: 147 6097
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5826 5858 4313
Size: 32 1481 32 4699
Fragments arranged by size:
4699 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2492 1613
Size: 879 5365
PsiI TTA'TAA
Cuts at: 2039 3814 2039
Size: 1775 4469
PspXI vC'TCGA_Gb
Cuts at: 2490 2490
Size: 6244
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2811 2843 2811
Size: 32 6212
PstI C_TGCA'G
Cuts at: 830 3596 830
Size: 2766 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
PvuII CAG'CTG
Cuts at: 2984 2984
Size: 6244
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SbfI CC_TGCA'GG
Cuts at: 3596 3596
Size: 6244
ScaI AGT'ACT
Cuts at: 4492 6204 4492
Size: 1712 4532
SfcI C'TryA_G
Cuts at: 826 1045 2341 3592 4727 5408 5599 826
Size: 219 1296 1251 1135 681 191 1471
Fragments arranged by size:
1471 1296 1251 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 3571 1872
Size: 1699 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 2490 4348 5249 5511 820
Size: 197 926 309 238 1858 901 262 1553
Fragments arranged by size:
1858 1553 926 901 309 262 238 197
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 3361 4168 5
Size: 47 3309 807 2081
Fragments arranged by size:
3309 2081 807 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6244
StyI C'CwwG_G
Cuts at: 513 1065 2497 2529 2610 3219 3683 513
Size: 552 1432 32 81 609 464 3074
Fragments arranged by size:
3074 1432 609 552 464 81 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2284 2307 2561 4271 4288 4441 4626
Size: 1255 704 23 254 1710 17 153 185
Cuts at: 4626 5071 325
Size: 445 1498
Fragments arranged by size:
1710 1498 1255 704 445 254 185 153
23 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3294 4490 6202
Size: 274 80 33 51 1443 1317 1196 1712
Cuts at: 6202 96
Size: 138
Fragments arranged by size:
1712 1443 1317 1196 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2208 2240 6204 6236 2208
Size: 32 3964 32 2216
Fragments arranged by size:
3964 2216 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6244
XbaI T'CTAG_A
Cuts at: 3580 3580
Size: 6244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2780 2780
Size: 6244
XhoI C'TCGA_G
Cuts at: 1943 2490 1943
Size: 547 5697
XmaI C'CCGG_G
Cuts at: 1870 3569 1870
Size: 1699 4545
XmnI GAAnn'nnTTC
Cuts at: 4373 4373
Size: 6244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4052 276
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI