Restriction map for
pFN21AE0038
(Circular) (Six-base) MAPSORT of: pFN21AE0038.seq Check: 2305 from: 1 to: 6811
pFN21AE0038 6811 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4621 278
Size: 53 83 186 936 3085 2468
Fragments arranged by size:
3085 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3876 4154 1937
Size: 1939 278 4594
Fragments arranged by size:
4594 1939 278
Acc65I G'GTAC_C
Cuts at: 3659 4132 3659
Size: 473 6338
AclI AA'CG_TT
Cuts at: 1563 4110 4938 5311 1563
Size: 2547 828 373 3063
Fragments arranged by size:
3063 2547 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 4874 6258 6526 1632
Size: 402 2840 1384 268 1917
Fragments arranged by size:
2840 1917 1384 402 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6614
AflIII A'CryG_T
Cuts at: 1203 2511 3797 4073 5710 6687 1203
Size: 1308 1286 276 1637 977 1327
Fragments arranged by size:
1637 1327 1308 1286 977 276
AgeI A'CCGG_T
Cuts at: 5642 5642
Size: 6811
AhdI GACnn_n'nnGTC
Cuts at: 5540 5540
Size: 6811
AleI CACnn'nnGTG
Cuts at: 1166 2512 3567 1166
Size: 1346 1055 4410
Fragments arranged by size:
4410 1346 1055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4113 1184
Size: 2929 3882
AlwNI CAG_nnn'CTG
Cuts at: 4554 6126 4554
Size: 1572 5239
ApaLI G'TGCA_C
Cuts at: 4868 6024 4868
Size: 1156 5655
ApoI r'AATT_y
Cuts at: 1493 2151 2233 2404 2877 3200 3230 3602 3756
Size: 658 82 171 473 323 30 372 154
Cuts at: 3756 3953 4019 4120 4346 4498 6607 1493
Size: 197 66 101 226 152 2109 1697
Fragments arranged by size:
2109 1697 658 473 372 323 226 197
171 154 152 101 82 66 30
AseI AT'TA_AT
Cuts at: 160 1032 2391 2436 5365 160
Size: 872 1359 45 2929 1606
Fragments arranged by size:
2929 1606 1359 872 45
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
AvaI C'yCGr_G
Cuts at: 1870 1943 4136 1870
Size: 73 2193 4545
Fragments arranged by size:
4545 2193 73
BamHI G'GATC_C
Cuts at: 4141 4508 4141
Size: 367 6444
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3659 4132 5587 618
Size: 325 217 627 1872 473 1455 1842
Fragments arranged by size:
1872 1842 1455 627 473 325 217
BanII G_rGCy'C
Cuts at: 1391 3784 4130 1391
Size: 2393 346 4072
Fragments arranged by size:
4072 2393 346
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2518 928
Size: 971 619 5221
Fragments arranged by size:
5221 971 619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5002 5036 1903
Size: 34 3065 34 3678
Fragments arranged by size:
3678 3065 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4703 5913 4703
Size: 1210 5601
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6811
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5422 136
Size: 107 122 71 631 605 3750 1525
Fragments arranged by size:
3750 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2725 6806 2725
Size: 4081 2730
BlpI GC'TnA_GC
Cuts at: 4228 4228
Size: 6811
Bme1580I G_kGCm'C
Cuts at: 1790 4872 6028 1790
Size: 3082 1156 2573
Fragments arranged by size:
3082 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6811
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2690 4542 5500
Size: 416 53 83 399 24 1273 1852 958
Cuts at: 5500 442
Size: 1753
Fragments arranged by size:
1852 1753 1273 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6811
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3524 5471 1239
Size: 2285 1947 2579
Fragments arranged by size:
2579 2285 1947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4936 5801 6099 3463
Size: 1473 865 298 4175
Fragments arranged by size:
4175 1473 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3542 5474 882
Size: 617 2043 1932 2219
Fragments arranged by size:
2219 2043 1932 617
BsaAI yAC'GTr
Cuts at: 493 1204 2707 4602 493
Size: 711 1503 1895 2702
Fragments arranged by size:
2702 1895 1503 711
BsaBI GATnn'nnATC
Cuts at: 2919 3472 6805 2919
Size: 553 3333 2925
Fragments arranged by size:
3333 2925 553
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4618 5000 5635 275
Size: 53 83 186 936 3085 382 635 1451
Fragments arranged by size:
3085 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5244 5642 5916 6063 6474 5244
Size: 398 274 147 411 5581
Fragments arranged by size:
5581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3086 3116 1178
Size: 30 165 30 1683 30 4873
Fragments arranged by size:
4873 1683 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6811
BseYI C'CCAG_C
Cuts at: 3907 6014 6510 3907
Size: 2107 496 4208
Fragments arranged by size:
4208 2107 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6238
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4261 5022 6050 1540
Size: 275 186 2260 761 1028 2301
Fragments arranged by size:
2301 2260 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2735 3832 4872 4957 6028 2735
Size: 1097 1040 85 1071 3518
Fragments arranged by size:
3518 1097 1071 1040 85
BsmI GAATG_Cn'
Cuts at: 2647 3578 4322 4415 2647
Size: 931 744 93 5043
Fragments arranged by size:
5043 931 744 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2735 3784 3832 4130 4872 4957 6028
Size: 399 945 1049 48 298 742 85 1071
Cuts at: 6028 1391
Size: 2174
Fragments arranged by size:
2174 1071 1049 945 742 399 298 85
48
BspEI T'CCGG_A
Cuts at: 6474 6474
Size: 6811
BspHI T'CATG_A
Cuts at: 4700 6430 4700
Size: 1730 5081
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4166 844
Size: 3322 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4698 1394
Size: 429 2875 3507
Fragments arranged by size:
3507 2875 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2083 2888 3723 5306 5480 65
Size: 1183 835 805 835 1583 174 1396
Fragments arranged by size:
1583 1396 1183 835 835 805 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3311 5455 5642 1700
Size: 110 1501 2144 187 2869
Fragments arranged by size:
2869 2144 1501 187 110
BsrGI T'GTAC_A
Cuts at: 96 3593 96
Size: 3497 3314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6811
BssSI C'ACGA_G
Cuts at: 4871 5883 4871
Size: 1012 5799
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3122 794
Size: 2328 4483
BstBI TT'CG_AA
Cuts at: 5615 5615
Size: 6811
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6811
BstYI r'GATC_y
Cuts at: 1972 2267 2517 2684 2725 4141 4508 4893 4910
Size: 295 250 167 41 1416 367 385 17
Cuts at: 4910 6362 6448 6460 6806 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1416 385 367 346 295 250
167 86 41 17 12
Bsu36I CC'TnA_GG
Cuts at: 2751 2751
Size: 6811
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5320
Fragments arranged by size:
5320 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6072
Fragments arranged by size:
6072 683 56
BtsI GCAGTG_nn'
Cuts at: 4327 5121 5141 4327
Size: 794 20 5997
Fragments arranged by size:
5997 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6811
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3805 3840 529
Size: 35 3241 35 3500
Fragments arranged by size:
3500 3241 35 35
DraI TTT'AAA
Cuts at: 3085 4115 3085
Size: 1030 5781
DraIII CAC_nnn'GTG
Cuts at: 2135 2135
Size: 6811
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5818 809
Size: 507 4502 1802
Fragments arranged by size:
4502 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4258 5147 6776
Size: 54 1425 50 275 18 2428 889 1629
Cuts at: 6776 8
Size: 43
Fragments arranged by size:
2428 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4258 1537
Size: 275 2446 4090
Fragments arranged by size:
4090 2446 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2197 4741 1353
Size: 844 2544 3423
Fragments arranged by size:
3423 2544 844
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5398 5772 5918 387
Size: 1064 138 3809 374 146 1280
Fragments arranged by size:
3809 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 3524 4874 5471 6258 6526 1239
Size: 393 402 1490 1350 597 787 268 1524
Fragments arranged by size:
1524 1490 1350 787 597 402 393 268
EcoRI G'AATT_C
Cuts at: 3756 4019 4120 4498 3756
Size: 263 101 378 6069
Fragments arranged by size:
6069 378 263 101
EcoRV GAT'ATC
Cuts at: 3255 3255
Size: 6811
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 6811
FspI TGC'GCA
Cuts at: 5317 5317
Size: 6811
HaeII r_GCGC'y
Cuts at: 1678 5958 1678
Size: 4280 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2174 2206 3174
Size: 32 135 32 458 32 112 32 968
Cuts at: 3174 3206 5452 5484 5526 5558 1373
Size: 32 2246 32 42 32 2626
Fragments arranged by size:
2626 2246 968 458 135 112 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3678 4155 4401 669
Size: 988 281 1740 477 246 3079
Fragments arranged by size:
3079 1740 988 477 281 246
HindIII A'AGCT_T
Cuts at: 748 2218 748
Size: 1470 5341
HpaI GTT'AAC
Cuts at: 4401 4401
Size: 6811
KpnI G_GTAC'C
Cuts at: 3663 4136 3663
Size: 473 6338
MfeI C'AATT_G
Cuts at: 3394 4410 3394
Size: 1016 5795
MluI A'CGCG_T
Cuts at: 6687 6687
Size: 6811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2749 3199 5925 6109 1305
Size: 1444 450 2726 184 2007
Fragments arranged by size:
2726 2007 1444 450 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4989
Fragments arranged by size:
4989 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2512 3360 3567 4769 5128 5287 518
Size: 648 1346 848 207 1202 359 159 2042
Fragments arranged by size:
2042 1346 1202 848 648 359 207 159
MspA1I CmG'CkG
Cuts at: 1606 3822 4227 4904 6052 6297 1606
Size: 2216 405 677 1148 245 2120
Fragments arranged by size:
2216 2120 1148 677 405 245
NaeI GCC'GGC
Cuts at: 3313 3313
Size: 6811
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5320
Fragments arranged by size:
5320 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6811
NgoMIV G'CCGG_C
Cuts at: 3311 3311
Size: 6811
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6811
NotI GC'GGCC_GC
Cuts at: 4258 4258
Size: 6811
NspI r_CATG'y
Cuts at: 1132 1229 2515 2897 3801 4077 4090 4169 5714
Size: 97 1286 382 904 276 13 79 1545
Cuts at: 5714 1132
Size: 2229
Fragments arranged by size:
2229 1545 1286 904 382 276 97 79
13
PacI TTA_AT'TAA
Cuts at: 2436 2436
Size: 6811
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6811
PciI A'CATG_T
Cuts at: 2511 3797 4073 5710 2511
Size: 1286 276 1637 3612
Fragments arranged by size:
3612 1637 1286 276
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2669 3425 3733 1108
Size: 1561 756 308 4186
Fragments arranged by size:
4186 1561 756 308
PmeI GTTT'AAAC
Cuts at: 4115 4115
Size: 6811
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4880 4912 6393 6425 4880
Size: 32 1481 32 5266
Fragments arranged by size:
5266 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6811
PsiI TTA'TAA
Cuts at: 3510 3740 4381 3510
Size: 230 641 5940
Fragments arranged by size:
5940 641 230
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3536 3568 4029 4061 3536
Size: 32 461 32 6286
Fragments arranged by size:
6286 461 32 32
PstI C_TGCA'G
Cuts at: 830 4163 830
Size: 3333 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6811
PvuII CAG'CTG
Cuts at: 3822 3822
Size: 6811
SalI G'TCGA_C
Cuts at: 1936 4153 1936
Size: 2217 4594
SbfI CC_TGCA'GG
Cuts at: 4163 4163
Size: 6811
ScaI AGT'ACT
Cuts at: 5059 6771 5059
Size: 1712 5099
SfcI C'TryA_G
Cuts at: 826 1045 3514 3891 4159 5294 5975 6166 826
Size: 219 2469 377 268 1135 681 191 1471
Fragments arranged by size:
2469 1471 1135 681 377 268 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6811
SmaI CCC'GGG
Cuts at: 1872 4138 1872
Size: 2266 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3478 4915 5816 6078 820
Size: 197 926 1535 1437 901 262 1553
Fragments arranged by size:
1553 1535 1437 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4602 493
Size: 4109 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6811
SphI G_CATG'C
Cuts at: 1132 4169 1132
Size: 3037 3774
SspI AAT'ATT
Cuts at: 5 52 4735 5
Size: 47 4683 2081
Fragments arranged by size:
4683 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6811
StyI C'CwwG_G
Cuts at: 513 1065 2004 2944 3972 4250 513
Size: 552 939 940 1028 278 3074
Fragments arranged by size:
3074 1028 940 939 552 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2299 3557 4838 4855 5008 5193 5638
Size: 1255 719 1258 1281 17 153 185 445
Cuts at: 5638 325
Size: 1498
Fragments arranged by size:
1498 1281 1258 1255 719 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3593 5057 6769
Size: 274 80 33 51 1443 1616 1464 1712
Cuts at: 6769 96
Size: 138
Fragments arranged by size:
1712 1616 1464 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6771 6803 6771
Size: 32 6779
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6811
XbaI T'CTAG_A
Cuts at: 4147 4147
Size: 6811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2572 2572
Size: 6811
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6811
XmaI C'CCGG_G
Cuts at: 1870 4136 1870
Size: 2266 4545
XmnI GAAnn'nnTTC
Cuts at: 2878 3340 3605 4940 2878
Size: 462 265 1335 4749
Fragments arranged by size:
4749 1335 462 265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4619 276
Size: 53 83 186 936 3085 2468
Fragments arranged by size:
3085 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PacI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI NruI
NsiI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI